• Title/Summary/Keyword: 식물병원균

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Isolation and Structure Determination of Phytotoxins from a Phytopathogenic Fungus Bipolaris sorokiniana (식물병원균 Bipolaris sorokiniana로부터 제초활성 물질의 분리 및 구조결정)

  • Lim, Chi-Hwan
    • Korean Journal of Agricultural Science
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    • v.29 no.2
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    • pp.91-97
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    • 2002
  • Two phytotoxic compounds, 3-methoxybenzoic acid (MBA) and 3-hydroxy benzoic acid methyl ester (HBAME), were purified and structurally characterized by instrumental analyses from a culture of a phytopathogenic fungus, Bipolaris sorokiniana. During the isolation procedure, the toxic components were monitored by the assay using rice (Echinochloa crusgalli) and Italian ryegrass (Lolium multiflorum). These compounds had a molecular formula of $C_8H_8O_3$ and inhibited the root growth of the plant seedlings over 90% at a level of 1000 ppm. This is the first report of herbicidal activity of MBA and HBAME purified from B. sorokiniana.

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Plant Cell Wall as an Inducer of Pectate Lyase of Erwinia rhapontice (Erwinia rhapontici의 Pectate Lyase를 유도하는 식물 세포벽)

  • 최재을;한광섭
    • Korean Journal Plant Pathology
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    • v.12 no.1
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    • pp.129-131
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    • 1996
  • Pecatate lyase(Pel)는 펙틴과 펙틴산을 분해하며, 기주식물의 감염에 관여한다. Erwinia rhapontici에 있어서 기주와 병원균이 병원성과의 상호관계를 구명하기 위하여 pectate lyase(Pel) 활성에 미치는 식물체 추출물과 세포벽의 효과를 검토하였다. 본 균은 glycerol이 포함된 minimal salts(MSG) 배지와 식물체 추출물이 첨가된 MSP 배지에서는 Pel 활성이 검출되지 않았다. 그러나 배추, 상추 잎, 감자 괴경, 셀러리 잎자루, 양파 인경, 당근 뿌리의 세포벽이 첨가된 MSP 배지에서는 Pel의 활성이 검출되었다. Pel을 유도하는 식물 인자는 불용성이고, 열처리에 불안전하였다.

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Survey of Overwintering Inoculum Potential of Anthracnose of Sweet Persimmon Caused by Colletotrichum gloeosporioides (감나무 탄저병균(Collectotrichum gloeosporioides)의 월동 전염원 조사)

  • Kwon, Jin-Hyeuk;Jeong, Seon-Gi;Chung, Bu-Keun
    • Research in Plant Disease
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    • v.13 no.3
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    • pp.204-206
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    • 2007
  • In 2006 to 2007, the potential inoculum source of the anthracnose of sweet persimmon caused by Colletotrichum gloeosporioides was surveyed. The infected twigs, buds, dead twigs, petiole, leaves, dropped fruits were collected and tested for their possibility as overwintering inoculum. The detection rates of the pathogen from various parts of sweet persimmon tree were varied. When the collected samples were examined in April. Over than 93.3% of infected twig samples were harbored mycelia of C. gloeosporioides, and 46.7% of infected buds, 36.7% of dead twigs, 23.3% of petioles, and 16.7% of leaves were beared pathogenic fungus. No pathogenic fungus were detecded from healthy twigs and buds. Infected twigs and bud was important overwintering sites and formed conidia actively in next spring. The infected twigs, leaves, petioles, and fruits in growing season produced great number of conidia and caused active dissemination of the anthracnose disease in sweet persimmon. In growing season, all of the infected parts, such as twigs, leaves, petioles, and fruits produced pathogenic fungus.

A Target Leaf Spot Disease Caused by Corynespora cassiicola on Cucumber Cultivated in Green House (시설하우스에서 Corynespora cassiicola에 의해 발생하는 오이 갈색무늬병)

  • Kwon, Mi-Kyung;Yang, Kwang-Yeol;Cho, Baik-Ho
    • Research in Plant Disease
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    • v.10 no.2
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    • pp.121-125
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    • 2004
  • An epidemic of target leaf spot of cucumber (Cucumis sativus) occurred in commercial greenhouses in Korea in 2000/2001. The early symptoms on the leaves were small brown spots with yellow halos. These lesions became irregular enlarging in diameter and eventually defoliation resulted. The causal agent was a fungus with morphological characteristics matching Corynespora cassiicola. The sequence of the ITS region of C. cassiicola CM2000-1 was identical to that of an authentic strain of Corynespora cassiicola. Optimal germination of spores and mycelial growth on plate was at 3$0^{\circ}C$. A long dew period on the leaf surface and high temperatures were the main contributing factors for disease development and the greenhouse epidemic. Artificial inoculation of the Korean isolate of C. cassiicola revealed resistance in some Korean cucumber cultivars.

One-step Multiplex RT-PCR Method for Simultaneous Detection of Seed Transmissible Bacterium and Virus Occurring on Brassicaceae Crop Seeds (십자화과 작물 종자에서 종자전염 세균 및 바이러스 동시 검출을 위한 One-step Multiplex RT-PCR 방법)

  • Jeong, Kyu-Sik;Soh, Eun-Hee
    • Research in Plant Disease
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    • v.17 no.1
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    • pp.52-58
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    • 2011
  • The aim of this research was to develop specific and sensitive PCR-based procedures for simultaneous detection of economically important plant pathogenic bacteria and seed borne virus in commercial Brassicaceae crop seeds, Xanthomonns campestris pv. campestris (Xcc) and Lettuce Mosaic Virus (LMV). Bacterial and virus diseases of Brassicaceae leaves are responsible for heavy losses. PCR with arbitral primers: selection of specific primers, performance of PCR with specific primers and determination of the threshold level for pathogens detection. To detect simultaneously the Xcc and LMV in commercial Brassicaceae crop seeds (lettuce, kohlrabi, radish, chinese cabbage and cabbage), two pairs of specific primer (LMV-F/R, Xcc-F/R) were synthesized by using primer-blast program (http://www.ncbi.nlm.nih.gov/tools/primer-blast/). The multiplex PCR for the two pathogens in Brassicaceae crop seeds could detect specifically without interference among primers and/or cDNA of other plant pathogens. The pathogen detection limit was determined at 1 ng of RNA extracted from pathogens. In the total PCR results for pathogen detection using commercial kohlrabi (10 varieties), lettuce (50 varieties), radish (20 varieties), chinese cabbage (20 varieties) and cabbage (20 varieties), LMV and Xcc were detected from 39 and 2 varieties, respectively. In the PCR result of lettuce, LMV and Xcc were simultaneously detected in 8 varieties.

Incidences and Causal Agents of Postharvest Fruit Rots in Kiwifruits in Korea (우리나라 참다래 저장병 발병율과 병원균)

  • Koh, Young-Jln;Lee, Jae-Goon;Hur, Jae-Seoun;Jung, Jae-Sung
    • Research in Plant Disease
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    • v.9 no.4
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    • pp.196-200
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    • 2003
  • Sixteen hundred fruits were randomly collected from 16 kiwifruit (Actinidia deliciosa) orchards in Jeonnam, Gyeongnam and Jejn provinces in Korea in 2000 and incidences of postharvest fruit rots were examined. The overall disease incidence was 32% and varied much with locations of orchards ranging from 5 to 68%. The percentages of kiwifruits showing internal, external, and both internal and external symptoms were 21.9%, 4.9%, and 5.2%, respectively. Several fungi were isolated from rotten fruits; Botryosphaeria dothidea, Diaporthe actinidiae and Botrytis cinerea were the major pathogens with the average isolation rates of 83.3%, 11.9% and 1.4%, respectively. Based on the symptoms on kiwifruits and the characteristics, the postharvest fruit rots caused by B. dothidea and D. actinidiae are suggested to be named as ripe rots and stem-end rots, respectively.