• Title/Summary/Keyword: 시험 평가

Search Result 11,017, Processing Time 0.038 seconds

Antimicrobial and Antioxidative Activities of the Extracts from Walnut (Juglans regia L.) Green Husk (호두과피 추출물의 항산화 및 항균활성)

  • Han, Kook-Il;Kim, Mi ran;Jo, Bu Kyung;Kim, Min Ji;Kang, Min Joo;Park, Ki-hyoun;Koo, Ye eun;Kim, Byeongseong;Jung, Eui-Gil;Han, Man-Deuk
    • Journal of Life Science
    • /
    • v.25 no.4
    • /
    • pp.433-440
    • /
    • 2015
  • Several studies suggest that regular consumption of walnuts may have beneficial effects against oxidative stress-mediated disease such as cancer. The present study reports the total phenolic and flavonoid contents, together with the antioxidant and antibacterial activities of several solvent extracts (methanol, n-hexane, ethyl acetate, n-butanol, and water) obtained from walnut (Juglans regia L.) green husk. MIC (minimal inhibitory concentration) values of the walnut extracts for 8 human pathogenic bacteria strain were determined using agar dilution method. Antioxidant activity of extracts were assessed using DPPH (1,1-diphenyl-2-picrylhydrazyl) and ABTS (2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulphonic acid)) assays, EC50 of DPPH and ABTS scavenging activities, and determination of total phenolic and flavonoid content and its correlation with DPPH and ABTS scavenging capacities. Among the six extracts, ethyl acetate extract (EtOAc Ex) showed the highest antimicrobial activity at 3.2 mg/ml of MICs against Staphylococcus aureus SG511. Total flavonoids and polyphenol contents of EtOAc Ex were 42.48 mg of quercetin equivalents (QE)/g and 223.25 mg of gallic acid equivalents (GAE)/g respectively. The highest antioxidative potential was shown by the sample extracted with EtOAc Ex (EC50=13.43 μg/ml for DPPH and EC50=41.83 μg/ml for ABTS radical scavenging activity assay). These results showed that J. regia green husk extracts can be used as an easily accessible source of natural antibacterial agents and natural antioxidants.

Seasonal and Inter-annual Variability of Water Use Efficiency of an Abies holophylla Plantation in Korea National Arboretum (국립수목원의 전나무(Abies holophylla) 조림지의 물 이용 효율의 계절 및 경년 변동)

  • Thakuri, Bindu Malla;Kang, Minseok;Zhang, Yonghui;Chun, Junghwa;Kim, Joon
    • Korean Journal of Agricultural and Forest Meteorology
    • /
    • v.18 no.4
    • /
    • pp.366-377
    • /
    • 2016
  • Water use efficiency (WUE) is considered as an important ecological indicator which may provide information on the process-structure relationships associated with energy-matter-information flows in ecosystem. The WUE at ecosystem-level can be defined as the ratio of gross primary productivity (GPP) to evapotranspiration (ET). In this study, KoFlux's long-term (2007-2015) eddy covariance measurements of $CO_2$ and water vapor fluxes were used to examine the WUE of needle fir plantation in Korea National Arboretum. Our objective is to ascertain the seasonality and inter-annual variability in WUE of this needle fir plantation so that the results may be assimilated into the development of a holistic ecological indicator for resilience assessment. Our results show that the WUE of needle fir plantation is characterized by a concave seasonal pattern with a minimum ($1.8-3.3g\;C{\cdot}(kg\;H_2O)^{-1}$) in August and a maximum ($5.1-11.4g\;C{\cdot}(kg\;H_2O)^{-1}$) in February. During the growing season (April to October), WUE was on average $3.5{\pm}0.3g\;C\;(kg\;H_2O)^{-1}$. During the dormant seasons (November to March), WUE showed more variations with a mean of $7.4{\pm}1.0g\;C{\cdot}(kg\;H_2O)^{-1}$. These values are in the upper ranges of WUE reported in the literature for coniferous forests in temperate zone. Although the growing season was defined as the period from April to October, the actual length of the growing season (GSL) varied each year and its variation explained 62% of the inter-annual variability of the growing season WUE. This is the first study to quantify long-term changes in ecosystem-level WUE in Korea and the results can be used to test models, remote-sensing algorithms and resilience of forest ecosystem.

Effects of ionizing and ultraviolet radiation on microbial mutation and DNA damage (전리방사선 및 자외선의 미생물 돌연변이와 DNA 손상에 대한 영향)

  • Nam, Ji-Hyun;Shin, Ji-Hye;Lee, Jung-Yun;Lee, Dong-Hun
    • Korean Journal of Microbiology
    • /
    • v.53 no.1
    • /
    • pp.20-28
    • /
    • 2017
  • Physical sterilization methods using ultraviolet radiation and ionizing radiation such as gamma ray and electron beam are applied in various industry fields due to disinfection effects and economic efficiency but may also cause microbial mutation. In this research, Salmonella enterica and Escherichia coli strains were treated with ionizing and ultraviolet radiation and their survival rate, mutation rate, and DNA damage were studied to evaluate the genetic safety. The survival rate of the strains decreased drastically as the irradiation dose of ultraviolet ray, gamma ray, and electron beam increased, and over 90% of the strain was exterminated at a dosage of $0.40{\sim}25.06mJ/cm^3$, 0.11~0.22 kGy, 0.14~0.53 kGy respectively. In SOS / umu-test, genotoxicity causing DNA damage was identified in all samples. In Ames test, back-mutation rate increased to $3.82{\times}10^{-4}$ and $9.84{\times}10^{-6}$ respectively when exposed to ultraviolet ray and gamma ray. At exposure to ultraviolet ray, gamma ray, and electron beam with dosage of over 99.99% extinction rate of S. enterica TA100, back-mutation rate increased 347 times, 220 times, 0.6 times respectively to the spontaneous back-mutation rate. Rifampicin resistance mutation rate of E. coli CSH100 exposed to ultraviolet ray, gamma ray, and electron beam was $2.46{\times}10^{-6}$, $1.66{\times}10^{-6}$, $4.12{\times}10^{-7}$ respectively. Therefore, gamma radiation is effective in microorganism control from the perspective of disinfection and electron beam has the advantage of sterilizing with little DNA damage and bacterial mutation.

Effect of Roasted Ground Coffee Residue Extract on Shelf-life and Quality of Salted Mackerel (커피박 추출물이 간고등어의 저장성과 품질에 미치는 영향)

  • Song, Eu-Jin;Kim, Jin-Yul;Lee, So-Young;Kim, Koth-Bong-Woo-Ri;Kim, Seo-Jin;Yoon, So-Young;Lee, So-Jeong;Lee, Chung-Jo;Ahn, Dong-Hyun
    • Journal of the Korean Society of Food Science and Nutrition
    • /
    • v.38 no.6
    • /
    • pp.780-786
    • /
    • 2009
  • To develop a novel application for roasted ground coffee residue, hot water extract of roasted ground coffee residue was used in manufacturing salted mackerels. First, DPPH radical scavenging effect of roasted ground coffee residue extract was measured. As a result, roasted ground coffee residue extract showed high activity of about 92% in the concentration of 0.4 to 4 mg/mL. Thereafter, the salted mackerel was treated with 5, 10 and 15% of roasted ground coffee residue extract and its shelf-life and quality was investigated. The salted mackerels treated with 15% roasted ground coffee residue extract showed significantly low TBARS and VBN as compared to the control. In sensory evaluation, the salted mackerels treated with 10 and 15% roasted ground coffee residue extracts scored higher as compared to the control. In conclusion, roasted ground coffee residue extract increased the shelf-life of salted mackerel and improved the sensual quality by inhibiting lipid oxidation.

Thinning Intensity Effects on Carbon Storage of Soil, Forest Floor and Coarse Woody Debris in Pinus densiflora Stands (간벌 강도가 소나무림의 토양, 낙엽층 및 고사목 탄소 저장량에 미치는 영향)

  • Ko, Suin;Yoon, Tae Kyung;Kim, Seongjun;Kim, Choonsig;Lee, Sang-Tae;Seo, Kyung Won;Son, Yowhan
    • Journal of Korean Society of Forest Science
    • /
    • v.103 no.1
    • /
    • pp.30-36
    • /
    • 2014
  • This study examined the change in carbon (C) storage of soil, forest floor and coarse woody debris (CWD) for different thinning intensities in Pinus densiflora stands 4 years after the treatment. Two study stands were located in Jeongseon (Stand 1) and Gwangneung Experiment Forest (Stand 2). Three plots for different thinning intensities based on stand density were established at each stand in 2008; control plot (0%), T20 plot (20%) and T30 plot (30%) in Stand 1 and control plot (0%), T39 plot (39%) and T74 plot (74%) in Stand 2, respectively. The C storage of soil (0-50 cm), forest floor and CWD was measured in 2012. Total C storage of T30 plot ($109.80t{\cdot}C{\cdot}ha^{-1}$) was significantly higher than that of control plot ($86.69t{\cdot}C{\cdot}ha^{-1}$) in Stand 1. In stand 2, total C storage of T74 plot ($97.02t{\cdot}C{\cdot}ha^{-1}$) was significantly higher than that of control plot ($72.04t{\cdot}C{\cdot}ha^{-1}$) and T39 plot ($63.25t{\cdot}C{\cdot}ha^{-1}$). Total C storage of the heaviest thinned plot was the highest in each study stand. Since this study examined initial effects of thinning on C storage of soil, forest floor and CWD, further studies would be necessary to evaluate the long-term effects.

Comparison of flexural strength according to thickness between CAD/CAM denture base resins and conventional denture base resins (CAD/CAM 의치상 레진과 열중합 의치상 레진의 두께에 따른 굴곡 강도 비교)

  • Lee, Dong-Hyung;Lee, Joon-Seok
    • Journal of Dental Rehabilitation and Applied Science
    • /
    • v.36 no.3
    • /
    • pp.183-195
    • /
    • 2020
  • Purpose: The purpose of this study is to compare the flexural strength of CAD/CAM denture base resins with conventional denture base resins based on their thicknesses. Materials and Methods: For the conventional denture base resins, Lucitone 199® (C-LC) was used. DIOnavi - Denture (P-DO) and DENTCA Denture Base II (P-DC) were taken for the 3D printing denture base resins. For the prepolymerized PMMA resins, Vipi Block Gum (M-VP) and M-IVoBase® CAD (M-IV) were used. The final dimensions of the specimens were 65.0 mm x 12.7 mm x 1.6 mm / 2.0 mm / 2.5 mm. The 3-point bend test was implemented to measure the flexural strength and flexural modulus. Microscopic evaluation of surface of fractured specimen was conducted by using a scanning electron microscope (SEM). After testing the normality of the data, one-way ANOVA was adopted to evaluate the differences among sample groups with a significance level of P = 0.05. The Tukey HSD test was performed for post hoc analysis. Results: Under the same thicknesses, there are significant differences in flexural strength between CAD/CAM denture base resins and conventional denture base resins except for P-DO and C-LC. M-VP showed higher flexural strength than conventional denture base resins, P-DC and M-IV displayed lower flexural strength than conventional denture base resins. Flexural modulus was highest in M-VP, followed by C-LC, P-DO, P-DC, M-IV, significant differences were found between all materials. In the comparison of flexural strength according to thickness, flexural strength of 2.5 mm was significantly higher than that of 1.6 mm in C-LC. Flexural strength of 2.5 mm and 2.0 mm was significantly higher than that of 1.6 mm in P-DC and M-VP. In M-IV, as the thickness increases, significant increase in flexural strength appeared. SEM analysis illustrates different fracture surfaces of the specimens. Conclusion: The flexural strength of different CAD/CAM denture base resins used in this study varied according to the composition and properties of each material. The flexural strength of CAD/CAM denture base resins was higher than the standard suggested by ISO 20795-1:2013 at a thickness of 1.6 mm or more though the thickness decreased. However, for clinical use of dentures with lower thickness, further researches should be done regarding other properties at lower thickness of denture base resins.

CLINICAL STUDY OF SHADE IMPROVEMENT AND SAFETY OF POLYMER-BASED PEN TYPE BlancTis Forte WHITENING AGENT CONTAINING 8.3% CARBAMIDE PEROXIDE (8.3% Carbamide Peroxide 함유 펜 형 자가미백제인 BlancTis Forte의 색조개선 및 안전성에 관한 임상연구)

  • Lee, Jin-Kyung;Min, Sun-Hong;Hong, Sung-Tae;Oh, So-Ram;Chung, Shin-Hye;Hwang, Young-Hye;You, Sung-Yeop;Bae, Kwang-Shik;Baek, Seung-Ho;Lee, Woo-Cheol;Son, Won-Jun;Kum, Kee-Yeon
    • Restorative Dentistry and Endodontics
    • /
    • v.34 no.2
    • /
    • pp.154-161
    • /
    • 2009
  • This clinical study evaluated the whitening effect and safety of polymer based-pen type BlancTis Forte (NIBEC) containing 8.3% carbamide peroxide. Twenty volunteers used the BlancTis Forte whitening agent for 2 hours twice a day for 4 weeks. As a control. Whitening Effect Pen (LG) containing 3% hydrogen peroxide was used by 20 volunteers using the same protocol. The change in shade (${\Delta}E^*$, color difference) was measured using $Shadepilot^{TM}$ (DeguDent) before, during, and after bleaching (2 weeks, 4 weeks, and post-bleaching 4 weeks). A clinical examination for any side effects (tooth hypersensitivity or soft tissue complications) was also performed at each check-up. The following results were obtained. 1. Both the experimental and control groups displayed a noticeable change in shade (${\Delta}E$) of over 2. No significant differences were found between the two groups (p > 0.05), implying that the two agents have a similar whitening effect.2. The whitening effect was mainly due to changes in a and b values rather than in L value (brightness). The experimental group showed a significantly higher change in b value, thus yellow shade, than the control (p < 0.05). 3. None of the participants complained of tooth hypersensitivity or soft tissue complications, confirming the safety of both whitening agents.

Detection of Phantom Transaction using Data Mining: The Case of Agricultural Product Wholesale Market (데이터마이닝을 이용한 허위거래 예측 모형: 농산물 도매시장 사례)

  • Lee, Seon Ah;Chang, Namsik
    • Journal of Intelligence and Information Systems
    • /
    • v.21 no.1
    • /
    • pp.161-177
    • /
    • 2015
  • With the rapid evolution of technology, the size, number, and the type of databases has increased concomitantly, so data mining approaches face many challenging applications from databases. One such application is discovery of fraud patterns from agricultural product wholesale transaction instances. The agricultural product wholesale market in Korea is huge, and vast numbers of transactions have been made every day. The demand for agricultural products continues to grow, and the use of electronic auction systems raises the efficiency of operations of wholesale market. Certainly, the number of unusual transactions is also assumed to be increased in proportion to the trading amount, where an unusual transaction is often the first sign of fraud. However, it is very difficult to identify and detect these transactions and the corresponding fraud occurred in agricultural product wholesale market because the types of fraud are more intelligent than ever before. The fraud can be detected by verifying the overall transaction records manually, but it requires significant amount of human resources, and ultimately is not a practical approach. Frauds also can be revealed by victim's report or complaint. But there are usually no victims in the agricultural product wholesale frauds because they are committed by collusion of an auction company and an intermediary wholesaler. Nevertheless, it is required to monitor transaction records continuously and to make an effort to prevent any fraud, because the fraud not only disturbs the fair trade order of the market but also reduces the credibility of the market rapidly. Applying data mining to such an environment is very useful since it can discover unknown fraud patterns or features from a large volume of transaction data properly. The objective of this research is to empirically investigate the factors necessary to detect fraud transactions in an agricultural product wholesale market by developing a data mining based fraud detection model. One of major frauds is the phantom transaction, which is a colluding transaction by the seller(auction company or forwarder) and buyer(intermediary wholesaler) to commit the fraud transaction. They pretend to fulfill the transaction by recording false data in the online transaction processing system without actually selling products, and the seller receives money from the buyer. This leads to the overstatement of sales performance and illegal money transfers, which reduces the credibility of market. This paper reviews the environment of wholesale market such as types of transactions, roles of participants of the market, and various types and characteristics of frauds, and introduces the whole process of developing the phantom transaction detection model. The process consists of the following 4 modules: (1) Data cleaning and standardization (2) Statistical data analysis such as distribution and correlation analysis, (3) Construction of classification model using decision-tree induction approach, (4) Verification of the model in terms of hit ratio. We collected real data from 6 associations of agricultural producers in metropolitan markets. Final model with a decision-tree induction approach revealed that monthly average trading price of item offered by forwarders is a key variable in detecting the phantom transaction. The verification procedure also confirmed the suitability of the results. However, even though the performance of the results of this research is satisfactory, sensitive issues are still remained for improving classification accuracy and conciseness of rules. One such issue is the robustness of data mining model. Data mining is very much data-oriented, so data mining models tend to be very sensitive to changes of data or situations. Thus, it is evident that this non-robustness of data mining model requires continuous remodeling as data or situation changes. We hope that this paper suggest valuable guideline to organizations and companies that consider introducing or constructing a fraud detection model in the future.

THE EFFECT OF CONCENTRATION AND APPLICATION TIME OF HYDROGEN PEROXIDE ON THE MICROTENSILE BOND STRENGTH OF RESIN RESTORATIONS TO THE DENTIN AT DIFFERENT DEPTHS (과산화수소의 농도와 적용시간이 상아질의 깊이에 따라 레진 수복물의 미세인장결합강도에 미치는 영향)

  • Son, Jeong-Lyong;Lee, Gye-Young;Kang, Yu-Mi;Oh, Young-Taek;Lee, Kwang-Won;Kim, Tae-Gun
    • Restorative Dentistry and Endodontics
    • /
    • v.34 no.5
    • /
    • pp.406-414
    • /
    • 2009
  • The purpose of this study was to examine the effect of hydrogen peroxide at different application time and concentrations on the microtensile bond strength of resin restorations to the deep and the pulp chamber dentin. A conventional endodontic access cavity was prepared in each tooth, and then the teeth were randomly divided into 1 control group and 4 experimental groups as follows: Group 1, non treated; Group 2, with 20% Hydrogen peroxide ($H_2O_2$); Group 3, with 10% $H_2O_2$; Group 4, with 5% $H_2O_2$; Group 5, with 2.5% $H_2O_2$; the teeth of all groups except group 1 were treated for 20, 10, and 5min. The treated teeth were filled using a Superbond C&B (Sun medical Co., Shiga, Japan). Thereafter, the specimens were stored in distilled water at $37^{\circ}C$ for 24-hours and then sectioned into the deep and the chamber dentin. The microtensile bond strength values of each group were analyzed by 3-way ANOVA and Tukey post hoc test(p < 0.05). In this study, the microtensile bond strength of the deep dentin (D1) was significantly greater than that of the pulp chamber dentin (D2) in the all groups tested. The average of microtensile bond strength was decreased as the concentration and the application time of $H_2O_2$ were increased. Analysis showed significant correlation effect not only between the depth of the dentin and the concentration of $H_2O_2$ but also between the concentration of H202 and the application time(p < 0.05), while no significant difference existed among these three variables(p > 0.05). The higher $H_2O_2$ concentration, the more opened dentinal tubules under a scanning electron microscope(SEM) examination.

프렌차이점에서 사용되는 튀김류의 산패도 및 트랜스지방의 함량 비교

  • Kim, Yeong-Seong
    • Proceedings of the Korean Sanitation Conference
    • /
    • 2005.12a
    • /
    • pp.76-97
    • /
    • 2005
  • As the recent change of multiformity and taste in clination in eating habit culture is yearly in creasing foods used oil and fats. Because the frying food is especially important snack , it's safty is very essential. In order to know the safty and harmfulness of frying oil and fats. The 20 kinds samples were purchased chicken fried food shops around the north of seoul and kyunggi. The acid value, iodine value, peroxide value, TBA value, fatty acid, carbonyl value, and smoke point of deep fat fried oils were analyzed. Results of analyzed, A company of deep fat frying oil showed stability state and C company and B company of deep fat frying oil is acidification to turned. But D company of deep fat frying oil showed quite a bit acidification progressived of used hydrogenated oil.

  • PDF