• Title/Summary/Keyword: 시험포

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Discrimination of Atractylodes Rhizome White Using Anatomical Characteristics and SCAR Markers (해부형태적 특징과 SCAR Marker를 이용한 백출의 기원식물 판별)

  • Bang, Kyong-Hwan;Sung, Jung-Sook;Park, Chung-Heon;Jin, Dong-Chun;Park, Chun-Geon;Yu, Hong-Seob;Park, Hee-Woon;Seong, Nak-Sul
    • Korean Journal of Medicinal Crop Science
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    • v.12 no.1
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    • pp.53-59
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    • 2004
  • Finding a means to discriminate the commercial herb medicines when they were dried and sliced is a very important and imminent project in Korea. To differentiate plant origins and the commercial herb medicines of Atractylodes japonica and A. macrocephala, two discriminative methods using anatomical characteristics and SCAR marker were applied. It was possible to discriminate plant origins and the commercial herb medicines between A. japonica and A. macrocephala by anatomical characteristics: development of periderm, layer of stone cell, distribution of laticiferous vessels, development of xylem fiber in xylem ray, contained quantity of clustered crystals and others. While, two SCAR markers were developed from RAPD clones: SAjR2 (600 bp) from AjR2 and SAmR1 (1,200 bp) from AmR1. These two markers were enough for discrimination plant origins and the commercial herb medicines between A. japonica and A. macrocephala. The result of application of anatomical characteristics and SCAR markers to investigate current status in domestic herb market, Daegu and Kumsan herb market, it was identified to be current herb medicines of A japonica.

Variation in Chromosome Number in Early Generation from Cross between Hexaploid Triticale(X Triticosecale Wittmack) and Wheat (Triticum aestivum L.) (6배체 트리티케일X밀 잡종 초기 세대의 염색체수 변이)

  • 황종진;이홍석
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.36 no.4
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    • pp.310-318
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    • 1991
  • This experiment was carried out to obtain the information on the variation of chromosome number in pollen mother cell (PMC) and somatic cell of the progeny from the cross between hexaploid triticale cv. Sinkihomil and five hexaploid wheat varieties. The results were summarized as follows: Number of uni-, bi- and tri-valent in PMC was 11.9, 14.4 and 0.44, respectively, in the F$_1$ between triticale and wheat. Significant positive correlation between the pollen fertility and seed set rate, pollen fertility and bivalent number of PMC, and seed set rate and bivalent number of PMC, and negative correlation between pollen fertility and uni-or tri-valent of PMC in the cross between triticale and wheat were detected. F$_1$ (crossed seed) had 42 chromosomes, F$_2$ and F$_1$/P$_1$ showed high frequency of hyperploid (42-49) and F$_1$/P$_2$ showed high frequency of hypoploid (36- 42), which suggest non-random segregation for somatic chromosome number. in the cross between the triticale and wheat.

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Polycyclic Aromatic Hydrocarbons in Agricultural Waterways in Gyeonggi and Gangwon Provinces, Korea (경기 및 강원지역 농업용수 중 PAHs의 모니터링 연구)

  • Kim, Leesun;Park, Byung-Jun;Lee, Sung-Eun
    • Korean Journal of Environmental Biology
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    • v.34 no.3
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    • pp.216-221
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    • 2016
  • Level and distribution of fourteen polycyclic aromatic hydrocarbons (PAHs) in the agricultural water samples collected in the waterways located in Gyeonggi and Gangwon, Korea were determined for monitoring and risk assessment. A simplified, fast but effective extraction and clean-up methods combined with gas chromatography tandem mass spectrometry (GC-MS/MS) was employed to measure the concentration of the target compounds. The extraction of the analytes of interest in water sample (10 mL) was performed with acetonitrile (10 mL) and the salt. To purify the target PAHs, the clean-up procedure was employed with 2 mL tubes of dispersive solid phase extraction. The optimized method was validated with recoveries, method detection limit (MDL), accuracy and precision. Good recoveries for each PAHs at 10 and $25{\mu}g\;L^{-1}$ were achieved (60 to 110%, with RSD <20%) with linearity (>0.99). MDL for all the analytes was achieved with $0.2{\mu}g\;L^{-1}$. GC-MS/MS results showed that concentration of phenanthrene in the water samples from Gyeonggi (20 sites) ranged from 0.82 to $2.56{\mu}g\;L^{-1}$ and from Gangwon (15 sites) ranged from 0.83 to $1.62{\mu}g\;L^{-1}$. Other PAHs were not found in the water samples but the continuous monitoring for these areas were required.

Anti-obesity Effects of Ethanolic Extract of Polygonatum sibiricum Rhizome in High-fat Diet-fed Mice (고지방식이로 비만이 유도된 마우스에서 황정 주정 추출물의 항비만 효과)

  • Ko, Jong-Hee;Jeon, Woo-Jin;Kwon, Hyuk-Sang;Yeon, Seung-Woo;Kang, Jae-Hoon
    • Korean Journal of Food Science and Technology
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    • v.47 no.4
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    • pp.499-503
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    • 2015
  • We investigated the anti-obesity effects of ethanolic extract (ID1216) of Polygonatum sibiricum rhizome and its potential underlying mechanism in an animal model. ID1216 treatment decreased body weight gain and white adipose tissue weight in the prevention study. The mRNA levels of sirtuin-1 (SIRT1), peroxisome proliferator-activated receptor ${\gamma}$ coactivator-$1{\alpha}$ ($PGC1{\alpha}$), and peroxisome proliferator-activated receptor ${\alpha}$ ($PPAR{\alpha}$) significantly increased in the epididymal white adipose tissue of ID1216-administered mice. The stimulation effects of ID1216 on these gene expressions were also observed in a cell-based assay using differentiated 3T3-L1 adipocytes. In addition, similar to orlistat, ID1216 treatment improved weight gain and reduced epididymal fat in the treatment model. These results suggest that ID1216 has potential as an anti-obesity agent by modulating the expression of genes related to thermogenesis, lipid metabolism and fatty acid oxidation.

Acid Rock Drainage Generation Capacity of Tertiary Mudstone in Pohang Basin (포항분지 제3기 이암의 산성배수 발생 능력)

  • Baek, In-Woo;Kim, Jae-Gon;Song, Young-Suk;Kim, Tae-Hyung
    • Journal of the Korean Geosynthetics Society
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    • v.19 no.2
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    • pp.23-33
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    • 2020
  • This study determines the basic properties and acid rock drainage generation capacity of Pohang tertiary mudstone through laboratory experiments. According to X-ray fluorescence (XRF) analysis results, the mudstone of this area mostly comprised of SiO2 with a proportion of approximately 60%, followed in order by Al2O3 and Fe2O3. As such, it is clear that there is an abundance of aluminosilicates with a high probability of generating acid rock drainage. The XRD analysis showed that the mudstone contains pyrite (FeS2), it is highly likely to generate acid rock drainage, and inductively coupled plasma atomic emission spectroscopy (ICP-AES) analysis results showed that the mudstone samples contained a high amount of Fe2+ ions. As a result of anion analysis measured by ion chromatography (IC), all mudstone samples were measured to have high SO2-4 concentrations. According to elemental analysis, the total sulfur (S) content was high, which in turn indicates a high risk of acid rock drainage generation reflected by a maximum potential acidity (MPA) higher than 1%. All in all, although there were slight deviations between the tertiary mudstone samples, overall, the samples exhibited high acid rock drainage generation capacities.

Properties of Wine from Domestic Grape, Vitis labrusca cultivar. Campbell's Early, Fermented by Carbonic Maceration Vinification Process (Carbonic maceration 발효 방법으로 제조된 국내산 포도, Vitis labrusca cultivar. Campbell's Early의 포도주 특성)

  • Park, Won-Mok;Park, Hyuk-Gu;Rhee, Sook-Jong;Kang, Kyung-Il;Lee, Cherl-Ho;Yoon, Kyung-Eun
    • Korean Journal of Food Science and Technology
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    • v.36 no.5
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    • pp.773-778
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    • 2004
  • Properties of wine produced from domestic grape, cultivar Campbell's Early, by carbonic maceration vinification process (CM) were investigated. Process required whole grape clusters without crushing or destemming and no yeast inoculation for fermentation. Fermentation heat was not generated. Yield of wine was 77%, close to that obtained by conventional process, 76%. Acidities of CM wines, free run and pressed wines, and conventional wine were pH 3.6 and 3.3, respectively. Tartaric acid contents of conventional, free run, and pressed wines were 1,813, 4,691, and 5,633 ppm, while those of malic acid were 3,446, 2,077, and 2,275 ppm, respectively. CM could reduce malic acid content by 2/3 that of conventional process. Both processes gave almost equal amounts of citric and acetic acids. CM wines had intense grape aroma and deep purple-blue, natural grape color.

Mechanical Properties of Recycled Powder mixing Concrete (재생미분말을 혼입한 콘크리트의 역학적 특성)

  • Lee, Seung-Hwan;Jung, Dae-Jin;Choi, Ik-Chang
    • Proceedings of the Korea Concrete Institute Conference
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    • 2008.04a
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    • pp.769-772
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    • 2008
  • The problem of disposing construction waste materials has become a national and social problem. Recycled powder generated in the process of making aggregate, and the recycled powder is land-filled in its entirety. Results of toxicity testing of recycled power show that it contains base-pair substituent mutagenicity. As recycled powder is disposed of as landfill, it can cause secondary contamination such as soil and underground water contamination. There has been very little research made on recycled powder. This study has examined the utilization of concrete mixture by using recycled powder in a mixture instead of cement and compared and analyzed the characteristics of dynamics and workability. This study has examined the application of recycled powder in concrete. Depending on the replacement rate and workability, test piece was manufactured using different mixing rate by CP, WCP, PCP. The CP was used to examine the physical property of concrete and characteristics its dynamics. The letters W of WCP and P of PCP are the initials of water and mixture. They were made using the standard mixing ratiosemphasizing the workability to determine the characteristic of dynamics of concrete based on the mixing ratio of recycled powder. With the increase in the replacement rate, CP had very little change in the strength. But with the decline of slump, the workability was not good. The result of manufacturing WCP and PCP using the standard mixing ratio showed that WCP had a drop in strength compared to the plain. PCP had almost the same value as the plain only when the replacement rate was 10%. When it was higher than that, a reduction in strength was observed.

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Comparison of Labour and Growth Characters of Grape cv. 'Campbell Early' between Wakeman and Modified-T Trellis Training Systems (포도 '캠벨얼리'의 웨이크만과 개량일자형 수형에서의 노동력과 생장특성 비교)

  • Park, Seo-Jun;Cho, Eun-Kyung;Kim, Su-Jin;Hur, Youn-Young;Nam, Jong-Chul;Park, Jeong-Kwan;Hwang, Hae-Sung;Jung, Sung-Min
    • Journal of Bio-Environment Control
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    • v.25 no.3
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    • pp.206-211
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    • 2016
  • Modified T trellis (MT) and Wakeman trellis (WT) were widely used Korean vineyard because they had an advantage for spur pruning type cultivar such as Campbell Early. In this experiment, we compared labor time and intensity for bunch management between MT and WT trellis systems in 'Campbell Early' grapes. As a result, berry thinning was required 17.3 hours (10a) on the WT trellis but was required 12.3 hours (10a) on the MT trellis. In like manner, bagging was required 10.1 hours (10a) on the WT trellis but was required 8.2 hours (10a) on the MT trellis. On the other hand, labor intensity measured on berry thinning and bagging practices using REBA (Rapid Entire Body Assessment) index, then WT trellis was scored 13.0, but MT trellis was scored 8.6. Meanwhile, MT trellis reduced vigorous growing of internodes length and width on grapevine shoots. Consequently, MT trellis is more convenience trellis for working ergonomically in Korea vineyard.

Chemical compatibility of interim material and bonding agent on shear bond strength (임시수복 재료와 본딩제의 화학적 호환성이 전단결합강도에 미치는 영향)

  • Lee, Jonghyuk
    • Journal of Dental Rehabilitation and Applied Science
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    • v.32 no.4
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    • pp.293-300
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    • 2016
  • Purpose: The purpose of this study is finding proper bonding agents to be used when adding bis-acryl composite provisional materials. Materials and Methods: Three bonding agents with different chemical compositions were included in this study. Forty disk shaped specimens of bis-acryl composite provisional material were prepared and divided into 4 groups according to the bonding agents. Control group didn't have bonding agent. Through the Teflon mould with 4.0 mm diameter hole with 4.0 mm thickness the same bis-acryl composite provisional material was added on the disks after the surface of each specimen was treated with designated bonding agent according to the manufacturer's instructions. Shear bond test was performed and the fractured surfaces were inspected with a microscope. One-way analysis of variance was conducted and the result was further analysed with Turkey post hoc test at the significance level of 0.05. Results: The highest strength was acquired from the specimens bonded with chemical cure system and it was statistically significant (P < 0.05). This group showed 100% cohesive failures. The lowest bonding strength was recorded from the specimens used conventional light cure bonding agent, and this group's result was similar with the control group. The group used a light cure bonding agent claiming improved compatibility revealed significantly higher bond strength to the traditional light cure bonding agent group in a statistically significant way (P = 0.043). Conclusion: According to the bonding agent used the shear bond strength was significantly affected. Therefore the choice of proper bonding agent is important when hiring a bonding agent to add bis-acryl composite provisional materials.

Standardization of Hydration in the Stratum Corneum Using by Polyols (폴리올을 이용한 각질층 수분량 측정의 표준화 연구)

  • Nam, Gaewon
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.41 no.2
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    • pp.113-119
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    • 2015
  • The measurement of hydration level in the surface layer of the skin, stratum corneum (SC), gives important information on the biophysical properties and function of the skin barrier such as softness, flexibility, and healthiness of the skin. But it is difficult to measure a consistent hydration level from a sample to another sample due to individual variations and environmental changes. The aim of this study was to evaluate objective hydration after using various products in the SC. The SC Hydration was measured by capacitance (Corneometer$^{(R)}$, C+K, Germany) on the ventral site of forearm from 40 healthy volunteers. The skin surface was chronologically measured immediately after application of the test products and 3 and 6 hours later. We analyzed the averages of five measurements of each site and used the hydration increase rate for correction on untreated site variation. We found that most polyols including glycerol and butylenes glycol influenced directly the hydration increase rate in the SC previously. In this study, glycerol was used to prepare the standard products from 0 to 20 percents and applied to the same volunteers. The individual standard curve showed linear relation to glycerol concentrations. Based on the the standard curve, hydration of SC was converted into hydration increase rate to glycerol concentrations. The converted glycerol concentrations of products were repetitive and reproducible. In addition, the individual standard curve was used to relate the skin type of each individual. These results suggest that the hydration of the SC standardized regardless of external variation and individual skin condition can explain detailed skin state variation. Further studies will be conducted with other ingredients such as surfactants, lipids and aqueous materials, and with other methods for noninvasive measurement.