• Title/Summary/Keyword: 시험조사선

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Genotoxicological Safety Evaluation of X-ray Irradiated Four Foods (X-선 조사식품 4종의 유전독성학적 안전성 평가)

  • Jung, Da-Woon;Huang, Yu-Hua;Song, Beom-Seok;Byun, Myung-Woo;Kang, Il-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.10
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    • pp.1588-1593
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    • 2014
  • This study evaluated the genotoxic effects of 30 kGy of X-ray irradiation to four foods (chicken, egg powder, dried green onion, and black pepper). In bacterial reversion assay with Salmonella Typhimurium TA98, TA100, TA1535, and TA1537, the X-ray irradiated foods did not show a significantly increased number of revertant colonies in the presence or absence of the S9 metabolic activation system. In chromosomal aberration tests with Chinese hamster ovary (CHO) cells, the X-ray irradiated foods showed no increase in the frequency of chromosomal aberrations. In in vivo mouse micronucleus assay, the X-ray irradiated foods did not show any increase in the frequency of polychromatic erythrocytes with micronuclei. These results indicate that 30 kGy of X-ray irradiation to four foods (chicken, egg powder, dried green onion, and black pepper) showed no genotoxic effects under these experimental conditions.

Safety Evaluation on Mutagenicity of White Layer Cake Containing Gamma-Irradiated Egg White (감마선 조사된 난백 함유 White Layer Cake의 돌연변이원성 평가)

  • 김미정;이주운;서지현;송현파;육홍선;최정미;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.32 no.7
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    • pp.1172-1175
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    • 2003
  • Mutagenicity of white layer cake including 20 kGy-gamma irradiated egg white manufactured as a research on the practical approaches of gamma irradiation for the reduction of egg allergy was evaluated by Salmonella typhimurium reversion assay (Ames test). The water-soluble and organic solvents mixture of methanol: chloroform (2 : 1)-soluble fractions of the white layer cake including 20 kGy-gamma irradiated egg white were examined in S. typhimurium TA98 and TA100. Both with and without metabolic activation, the number of revertant colonies were not increased in each extract compared with negative controls. No significant difference in the formation of the colonies was observed at the non-irradiated and 20 kGy-irradiated samples. The results indicate that there is no evidence of mutagenicity in white layer cake including 20 kGy-gamma irradiated egg white.

The Implementation of Integrated Information Network for JANG-MOK Oceanographic Research Ship (연안해역 해양특성 연구조사선 장목호의 구축현황)

  • Park Dong-Won;Park Jong-Won;Kim Dug-Jin;Baek Hyuk
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2006.05a
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    • pp.514-516
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    • 2006
  • KORDI(Korea Ocean Research & Development Institute) built a research vessel JANG-MOK with 40 G/T for a survey and observation of oceanographic environmental characteristics at coastal region in September 2005. This paper introduced specification, major loaded instruments, major survey and observation works of JANG-MOK research vessel.

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In vitro Genotoxicological Safety of Fresh Vegetable-Extract Juice by Gamma Irradiation (감마선 조사된 녹즙의 In vitro 유전독성학적 안전성 평가)

  • 이현자;강근옥;육홍선
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.30 no.6
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    • pp.1227-1236
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    • 2001
  • Genotoxicological safety on 10 kGy-gamma irradiated vegetable juices such as Oenanthstolonifera DC., Daucus carota L., Brassica oleracea var. acephala and Angelica keiskei was determined by the Salmonella typhmurium reversion assay, the SOS Chromotest using in Escherichia cloi PQ37 and chromosome aberration test in cultured Chinese hamster lung (CHL) fibroblast cells. Vegetable juices exposed to 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests.

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Genotoxicological Safety of Gamma-Irradiated Salted and Fermented Anchovy Sauce (감마선 조사된 멸치액젓의 유전독성학적 안전성 평가)

  • 육홍선;차보숙;김동호;이주운;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.7
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    • pp.1192-1200
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    • 2004
  • Gamma irradiations at 5 or 10 kGy were applied to salted and fermented anchovy sauce, for improving the hygiene Quality and evaluating the genotoxicological safety. In vitro genotoxicological safety of irradiated sauces was evaluated by Salmonella Typhimurium (TA98, TA100, TAI535 and TAI537) and E. coli WP2 uvrA, reversion assay, SOS chromotest (Escherichia coli PQ37), and chromosome aberration test (Chinese hamster lung fibroblast cells) in the absence or presence of an exogenous metabolizing system (S9 mix). The gamma-irradiated samples were not significantly different from nonirradiated-control for three in vitro tests (p<0.05). :In vivo micronucleus test using ICR mice (male) was not significantly different from the control at p<0.05. The salted and fermented anchovy sauce exposed to 5 or 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests and in vivo micronucleus test upto 50,000 $\mu$g/plate, respectively. The results indicated that 5 or 10 kGy gamma-irradiated salted and fermented anchovy sauces did not show any mutagenicity.

Study on the Estimation of Joint Length Distribution Using Window Sampling (조사창 조사를 이용한 절리 길이분포의 추정에 관한 연구)

  • 송재준;이정인
    • Journal of the Korean Geotechnical Society
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    • v.17 no.2
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    • pp.21-30
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    • 2001
  • 통계적 절리모델링에서 가장 불확실성이 큰 부분이 절리의 길이분포를 추정하는 것이다. 절리선 길이 분포의 추정에서 이제까지는 주로 조사선 조사(scanline survey)를 통한 절리선 반길이 분포를 이용하여 왔다. 이 연구에서는 포이송 디스크 절리모델에 대하여 보다 정밀도가 높은 절리선 길이 분포 추정방법을 찾기 위하여 조사창 조사를 이용하였다. 직사각형 및 원형 조사창에 대하여 양쪽 끝이 조사창 내부에 존재하는 절리선인 양끝내포선의 길이 분포와 한쪽 끝만이 존재하는 한끝내포선의 길이 분포를 이용, 절리선 분포를 추정하는 4개의 관계식을 각각 유도하고 컴퓨터 모의실험을 통하여 유도식의 타당성을 검증한 후 각 유도식을 이용한 절리선 분포의 추정오차를 비교하였다. 또한 절리선 분포로부터 절리직경분포를 계산하는 수치적 해를 유도하고 컴퓨터 모의시험을 통해 수치적 해에 대한 타당성을 검증하였다. 이 연구에서 제시한 조사창 조사를 이용한 절리 길이의 추정방법은 앞으로 절리모델링 분야에서 적용성이 높을 것으로 판단된다.

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Genotoxicological Safety of Gamma-Irradiated Kwamegi(semi-dried Colobabis seira) (감마선 조사된 과메기의 유전독성학적 안전성 평가)

  • 육홍선;정영진;송현파;이주운;변명우
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.33 no.1
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    • pp.182-192
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    • 2004
  • Gamma irradiation at 5 and 10 kGy was applied to Kwamegi (semi-dried Colobabis seira) for their possible hygiene quality and carried out genotoxicological safety. In vitro genotoxicological safety of each 5 and 10 kGy-irradiated Kwamegi was evaluated by Salmonella typhimurium (TA98, TA100, TA1535 and TA1537) and E. coli WP2 uvrA reversion assay, SOS chromotest (Escherichia coli PQ37) and chromosome aberration test (Chinese hamster lung fibroblast cells) in the absence and presence of an exogenous metabolizing system (S9 mix). Gamma-irradiated samples were not different from nonirradiated-control to respective in vitro tests. And in vivo micronucleus test using ICR mice (male) micronucleus was not observed. Kwamegi exposed to 10 kGy-gamma ray revealed negative results in these three in vitro mutagenetic tests and in vivo micronucleus test up to 10,000 $\mu\textrm{g}$/plate, respectively. The results indicated that 5 and 10 kGy gamma-irradiated Kwamegi (semi-dried Colobabis seira) did not have mutagenicity.

Genotoxicological Safety Evaluation of Imported Oranges Irradiated with Ionizing Energy (이온화 에너지를 조사한 수입 오렌지의 유전독성학적 안전성 평가)

  • Huang, Yu-Hua;Jung, Da-Woon;Kang, Il-Jun
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.43 no.6
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    • pp.909-915
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    • 2014
  • This study was carried out to evaluate the genotoxicity of imported oranges irradiated with ionizing energy (0.5 and 1 kGy). In bacterial reversion assays with Salmonella Typhimurium TA98, TA100, TA1535, and TA1537, imported oranges irradiated with ionizing energy (0.5 and 1 kGy) showed no significant increase in the number of revertant colonies in both the absence and presence of the S9 metabolic activation system. In chromosomal aberration tests with Chinese hamster ovary (CHO) cells, imported oranges irradiated with ionizing energy (0.5 and 1 kGy) showed no increase in the frequency of chromosomal aberrations. In in vivo mouse micronucleus assay, imported oranges irradiated with ionizing energy (0.5 and 1 kGy) showed no increase in the frequency of polychromatic erythrocytes with micronucleus. These results indicate that imported oranges irradiated with ionizing energy (0.5 and 1 kGy) showed no genotoxic effects under these experimental conditions.

Genotoxicological and Acute Toxicological Safeties of Gamma Irradiated Beef (감마선조사 쇠고기의 유전독성 및 급성독성학적 안전성평가)

  • Kang, Il-Jun;Kwak, Hee-Jin;Lee, Byung-Hoon;Kim, Kwang-Hoon;Byun, Myung-Woo;Yook, Hong-Sun
    • Korean Journal of Food Science and Technology
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    • v.30 no.4
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    • pp.775-780
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    • 1998
  • Gamma irradiation at 5 kGy was applied to beefs for evaluation of their possible genotoxicity and acute oral toxicity. The genotoxicity of 5 kGy irradiated beef was evaluated by Salmonella typhimurium reversion assay and in vivo micronucleus assay using mouse bone marrow cells. The results were negative in the bacterial reversion assay with S. typhimurium TA98, TA100, TA1535, TA1537. Clastogenic effects were not shown in vivo mouse micronucleus assay at 5 kGy dose tested. In an acute toxicity test, 5 kGy-irradiated beef was administrated orally at a dose level of 313 to 5,000 mg/kg, and then number of deaths, clinical signs, body weights, and pathological examinations were examined daily for 14 days post-administration. The results indicate that 5 kGy irradiated beef did not show any toxic effect on mice and oral $LD_{50}$ value was over 5,000 mg/kg on ICR mice.

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The Implementation of Integrated Information Network for JANG-MOK Oceanographic Research Ship (시험조사선 장목호의 종합정보통신망 구현)

  • Park Jong-Won;Kim Dug-Jin;Baek Hyuk;Park Dong-Won
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • 2006.05a
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    • pp.91-94
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    • 2006
  • KORDI(Korea Ocean Research & Development Institute) built a research vessel JANG-MOK with 40 G/T for a survey and observation of oceanographic environmental characteristics at coastal region in September 2005. This paper introduced the implementation for hardware and software of an integrated information network that loaded in JANG-MOK, and depicted the function of an integrated information network, such as an installation of ga-bit based network, RS232C serial & UDP network interface of instruments, a data logging software of measured data, Hawkeye II software for supporting the efficient survey works, and a real-time navigation viewer. In addition, we presents the another implementation method for an integrated information network of oceanographic research vessels.

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