• Microbiology and Biotechnology Letters
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• v.13 no.3
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• pp.179-184
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• 1985

Sexually compatible heterothallic haploids and diploids therefrom of Saccharomycopsis lipolytica were compared with respect to production. Diploids constructed through mating were confirmed by random spore analysis, whereas those constructed through protoplast fusion were confirmed by haploidization. ATCC 44601 and IFO 1209 produced larger amount of citrate and isocitrate than IFO 1550 and IFO 1551. A mode of citrate production by diploids was intermediate of the parental haploid strains. The specific activities of citrate synthase and isocitrate lyase in IFO 1550 and IFO 1551 were higher than those in ATCC 44601 and IFO 1209, indicating little correlation between citrate production and specific activities of these enzymes.

• Microbiology and Biotechnology Letters
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• v.14 no.4
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• pp.285-291
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• 1986

Immobilized Candida lipolytica cells were prepared by entrapping the whole cells in calcium alginate gel. To enhance citric acid productivity, immobilized cells were Incubated with activation medium in fluidized-bed reactors. When the activation was done in batch operation, maximum citric acid productivity appeared in a much shorter time than in continuous operation. Activated immobilized cells were enhanced about 10-fold in citric acid production relative to non-activated immobilized cells. The productivity of citric acid was also influenced by bead size. When Immobilized cells were reacted in a fluidized-bed reactor with the same quantity of cells, the citric acid productivity was increased as the bead size was decreased.

• Journal of the Korean Chemical Society
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• v.14 no.1
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• pp.91-96
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• 1970

Formation of the complexes of manganese, zinc and cobaltous ions with citrate ions in aqueous, ethanol-water (20% by volume) and acetone-water (20% by volume) solutions was studied at room temperature by the equilibrium exchange technique. The pH of the solutions was controlled to 7.0-7.4, and the ionic strength of the solutions was kept at approximately 0.10. The results of the present study indicated that both $Mn^{++}\;and\;Zn^{++}$ formed one to one citrate-compexes, [M Cit]$^-$, in all the solvent systems examined, and that $Co^{++}$ formed one to one citrate-complex in aqueous solution but formed both one to one citrate-complex, [Co Cit]$^-$, and one to two citrate-complex, $[Co\;Cit_2]^{4-}$, in the mixed solvent systems mentioned above. It was also observed that the citrate-complexes of the transition metals examined were more stable in the mixed solvent systems than in water.

• Applied Biological Chemistry
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• v.20 no.1
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• pp.117-122
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• 1977

Development of new burning additives against nitrate salts used in past tobacco industry examined to reduce harmful nitrogenous compounds in smoke. Among several additives treated, the best effect by means of elevation of combustibility and reduction of tar and nicotin contents in smoke was observed by adding sodium citrate from 0.6 to 1% in tabacco. The following results were obtained through investigating combustibility, chemical composition, and differential thermal analysis of the tabacco treated with the buring additives. 1) The close relation was observed between the levels of the smoke components and combustibility of the treated tabacco. 2) Differential thermal analysis (D.T.A.) of citrate-treated tobacco shows an increase in H. Value and a decrease in L. Value as compared with other tobacco sample. 3) The D.T.A. of sodium-nitrate and sodium-citrate shows that sodium-nitrate gives endothermal reaction at $270^{\circ}$ and $310^{\circ}$ and sodium citrate gives exothermal reaction at $290^{\circ}$ with endothermal reaction at $170^{\circ}\;and\;310^{\circ}$. 4) $Na_3-citrate-treated$ tobacco shows an increase in smoke generation between room temperature and $350^{\circ}$ (Zone A) and a decrease in smoke generation between $350^{\circ}$ and $900^{\circ}$ (Zone B) compared with untreated tobacco. The smoke from these tobacco also contains lower levels of Tar, Nicotine, Phenols, Nitrogen oxides and Benzpyrene, and elevation of static Burning rate.

• Korean Chemical Engineering Research
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• v.50 no.1
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• pp.173-176
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• 2012

Chlorine dioxide has an excellent ability to sterilize and deodorize and is harmless to humans. However, it is very unstable and explosive as it is highly concentrated, thus its use in the air clean filed has been limited. Therefore, there is a demand to develop the system to produce a low concentration of chlorine dioxide sustainedly. Here, for a first step in the development of the system on the sustained production of chlorine dioxide, the use of polymer hydrogels was investigated. P(MMA-co-HEMA) hydrogel particles were prepared via dispersion photopolymerization and sodium chlorite and citric acid were loaded respectively in the hydrogel particles. When sodium chlorite and citric acid were reacted with not loaded in the hydrogels, rapid production of chlorine dioxide occurred and the concentration of chlorine dioxide decreased over time. However, when sodium chlorite and citric acid were loaded respectively in the hydrogel particles and reacted, chlorine dioxide was produced slowly and sustainedly because the release of sodium chlorite and citric acid from the hydrogels delayed the reaction between them. The result shows that the use of P(MMA-co HEMA) hydrogels has the potential to develop the system on the sustained production of chlorine dioxide.

• Journal of the Korean Society of Propulsion Engineers
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• v.23 no.6
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• pp.21-27
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• 2019

Cellulose-based carbon fabrics are used in aerospace nozzles have low thermal conductivity and high ablation resistance. However, there is a disadvantage in that the weight is reduced by 70~90% in the pyrolysis process and graphitization process and the residual rate is low when the final carbon fabric is produced. In this study, phosphoric acid as a phosphorus flame retardant and Citric acid as a cross-linking agent were treated on the lyocell fabrics. After that the functional groups were identified and thermal properties were confirmed by FT-IR, XRD and TGA. The yields of the final carbon fabrics were also compared through the pyrolysis and graphitization process. The graphitized yield increased to 8.1% with increasing citric acid to 16 wt% added.

• Journal of Applied Biological Chemistry
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• v.64 no.2
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• pp.159-164
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• 2021

Heavy metals exist in soils in various chemical forms including free metal ions and organo-metal complexes. The ratio of free metal ions has been known to be highly associated with the plant absorption of heavy metals. This study aims to understand the effect of free ions and organo-metal complexes on the absorption of lead (Pb) and cadmium (Cd) by plants. For this, lettuce grown in a hydroponic system for 28 days was consequently grown another 48 hours using Pb and Cd solutions. The ratios of free ion to organo-metal complexes in the solutions were adjusted at 100:0, 90:10, 70:30, 60:40 by four different organic acids (citric, oxalic, acetic, and humic acid). After that, the concentration of Pb and Cd in lettuce were analyzed. The Pb and Cd absorption by lettuce was more relied on the types of organic acids treated and the type of metals rather than the ratio of free metal ions. For example, citric acid increased the Pb absorption while it decreased the Cd absorption by lettuce. There was no significant relationship between free metal ion ratios and both Pb and Cd uptake by lettuce. It could be explained that citric acid, a relatively higher molecular weight organic acid, has higher ion binding capacity, so it forms organo-Pb complex easily due to the higher affinity of Pb on the binding site in comparison with Cd. Consequently, this complexation would assist Pb uptake by lettuce.

• Journal of the Korean Chemical Society
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• v.14 no.1
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• pp.65-74
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• 1970

Formation of the complexes of alkaline earths with citrate ions in aqueous, acetone-water and ethanol-water solutions was studied at room temperature by the equilibrium exchange technique. This technique involved the measurements of distribution of radioactivity between cation exchange resin and solution phase after the radioactive metal ions were equilibriated with the cation exchange resin in the presence of citrate ions ($Cit^{3-}$) of varying concentrations. The pH of the solutions was controlled to 7.2-7.5, and the ionic strength of the solutions was kept at 0.10-0.12. The present study revealed that both barium and strontium ions formed the one to one citrate complexes, $[M Cit]^-$ in all solvent systems examined. It was also observed that calcium ions formed the one to one citrate complex in aqueous solution. In acetone-water and ethanol-water solutions, however, calcium ions were observed to form both one to one and one to two complexes, $[Ca Cit]^-$ and $[Ca\;Cit_2]^{4-}$. The results of the present study indicated also that relative stabilities of the complexes increased in the order; $Ba^{++}\;<\;Sr^{++}\;<\;Ca^{++}$, and in the order of increasing concentration of the organic components in the mixed solvent systems.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.2 no.2
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• pp.145-153
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• 2004

H$_2$SO$_4$ and citric acid were used as additives for the electrokinetic remediation experiment to increase removal efficiency of $^{137}$ Cs and $^{60}$ Co from the radioactive soil waste stored for more than 10 years. The average effluent velocity discharged from the elctrokinectic remediation experimental column was 2.0${\times}$10$^{-2}$ cm/min and the discharged soil wastewater volume for 10 days is 3.6 pore volume of the column. 97% of $^{60}$ Co in the column was decontaminated for 10 days of operation, while only 54% of $^{137}$ Cs was decontaminated. These results are considered that the absorption equilibrium coefficient of $^{137}$ Cs is higher than that of $^{60}$ Co. The predicted values of the residual concentration by the proposed mathematical model were well coincided with the experimental results within the experimental error range

• Korean Journal of Food Science and Technology
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• v.21 no.6
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• pp.863-868
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• 1989

In comparison of contents of the components related to the quality of kiwifruit depended on the cultivars, the obtained results were as follows; Abbott showed the highest values in soluble solid, crude fat, firmness and specific gravity. Potassium, phosphorus, calcium and magnesium were estimated as the major minerals in kiwifruit and Bruno contained the highest amounts of potassium and magnesium. Bruno also had high concentrations of ascorbic, maleic, citric and fumaric acid when compared with the other cultivars. The changes in chemical components of kiwifruit (Hayward) by ripening stage were as follows; During ripening, the glucose and fructose contents were increased with decrease of sucrose content. Ascorbic, maleic, fumaric and succinic acid contents were considerably increased during ripening From the investigation of changes in protein pattern by electrophoresis! the new bands with about 17,000-23,50kd were shown during ripening.