• Microbiology and Biotechnology Letters
• /
• v.40 no.2
• /
• pp.163-167
• /
• 2012

Since D-xylose is not fermentable in Saccharomyces cerevisiae, its conversion to D-xylulose is required for its application in biotechnological industries using S. cerevisiae. In order to convert D-xylose to D-xylulose by way of an enzyme immobilized system, D-xylose isomerase (XI) of Escherichia coli was fused with 10-arginine tag (R10) at its C-terminus for the simple purification and immobilization process using a cation exchanger. The fusion protein XIR10 was overexpressed in recombinant E. coli and purified to a high purity by a single step of cation exchange chromatography. The purified XIR10 was immobilized to a cation exchanger via the electrostatic interaction with the C-terminal 10-arginine tag. Both the free and immobilized XIR10 exhibited similar XI activities at various pH values and temperatures, indicating that the immobilization to the cation exchanger has a small effect on the enzymatic function of XIR10. Under optimized conditions for the immobilized XIR10, D-xylose was isomerized to D-xylulose with a conversion yield of 25%. Therefore, the results of this study clearly demonstrate that the electrostatic immobilization of XIR10 via the interaction between the 10-arginine tag and a cation exchanger is an applicable form of the conversion of D-xylose to D-xylulose.

• The Journal of the Korea Contents Association
• /
• v.10 no.6
• /
• pp.286-296
• /
• 2010

The study of the development of DJ robots is to meet the demands of the music services which are changing very rapidly in the digital and network era. Existing studies, as a whole, develop music services on the premise of personalized environment and equipment, but the DJ robot is on the premise of the open space shared by the public. DJ robot gives priority to traditional space and music. Recently as the hospitality and demand for cultural contents of South Korea expand to worldwide, industrial use of the contents based on traditional or our unique characteristics is getting more and more. Meanwhile, the DJ robot is composed of a combination of two modules. One is to detect changes in the external environment and the other is to set the properties of the music by psychology, emotional engineering, etc. DJ robot detect the footprint of the temperature, humidity, illumination, wind, noise and other environmental factors measured, and will ensure the objectivity of the music source by repeated experiments and verification with human sensibility ergonomics based on Hevner Adjective Circle. DJ robot will change the soundscape of the traditional space being more beautiful and make the revival and prosperity of traditional music with the use of traditional music through BGM.

• Microbiology and Biotechnology Letters
• /
• v.45 no.1
• /
• pp.81-86
• /
• 2017

Alignment of 967 reference sequences of the typical 2-Cys peroxiredoxin family of proteins revealed that 10 amino acids were conserved, with over 99% identity. To investigate whether the conserved aspartic acid residues and serine residue affect the peroxidase and chaperone activity of the protein, we prepared yeast TSA1 mutant proteins in which aspartic acids at positions 75 and 103 were replaced by valine or asparagine, and serine at position 73 was replaced by alanine. By non-reducing SDS-PAGE, TSA1 and the S73A, D75V and D75N mutants were detected in dimeric form, whereas the D103V and D103N mutants were detected in various forms, ranging from high molecular-weight to monomeric. Compared with wild type TSA1, the D75N mutant exhibited 50% thioredoxin peroxidase activity, and the S73A and D75V mutants showed 25% activity. However, the D103V and D103N mutants showed no peroxidase activity. All proteins, except for the D103V and D103N mutants, exhibited chaperone activity at $43^{\circ}C$. Our results suggest that the two conserved aspartic acid residues and serine residue of TSA1 play important roles in its thioredoxin peroxidase activity, and D103 plays a critical role in its chaperone activity.

• Journal of the Computational Structural Engineering Institute of Korea
• /
• v.27 no.4
• /
• pp.289-296
• /
• 2014

The rebar detailing is an important work influencing the final performance and quality of RC structures. But it is one of the most irrational and illogical activity in construction site. Many groups of workers, including main contractors, structural engineers, shop drawers, rebar fabricators, and etc., participate in this activity. A loosely-organized process for this activity is apt to produce a big amount of rebar loss or even degraded structures. A 3-dimensional rebar auto-placing system, called as Rebar Hub, has been designed and implemented in this research. Rebar Hub provides a totally integrated service from 3D structural modeling of buildings to rebar auto-placing considering anchorage, splice, and the length of ordered rebar. In addition, Rebar Hub can recognize the 2D drawing CAD files and then build 3D structural models which are used for the start point of 3D rebar auto-placing. After rebar auto-placing, each members of the 3D structural model have rebar information belonging to them. It means that the rebar information can be used for the afterward works such as quantity-survey, manufacturing and fabrication of rebars. Rebar Hub is showing outstanding performance while applying to practical projects. It has almost five times productivity and reduces the rebar loss up to 3~8% of the initially-surveyed amount of rebar.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2015.05a
• /
• pp.46-46
• /
• 2015

강우가 지역별 계절별로 편중되어 있는 우리나라는 수자원의 안정적인 확보와 이용을 위해 다양한 형태의 댐을 건설하여 운영하고 있다. 그러나 대부분의 댐건설을 통해 형성된 저수지들은 탁수 장기화 및 녹조 발생 등의 환경, 생태적인 문제를 겪고 있으며, 그에 따른 사회적 우려로 인해 신규댐 건설을 통한 수자원확보는 더 이상 어려운 실정이다. 이러한 문제에 대응하기 위한 대안으로 기존 댐 저수지들(안동호-임하호)의 구조적 연계운영방안이 진행되고 있다. 본 연구의 목적은 2차원 CE-QUAL-W2모형을 활용하여 안동호와 임하호의 구조적 연결에 따른 탁수의 이동과 각 저수지 내에서의 유동 변화를 해석하는데 있다. 저수지 연계 시나리오는 EL. 138 m 위치에 길이 2 km, 직경 5.5 m 의 콘크리트관(마찰계수 0.05)이 안동호 좌안인 임동면 마리와 임하호 우안 망천리를 연결하는 것으로 가정하였다. 모델의 보정은 실측자료가 풍부한 2006년도 수문사상을 대상으로, 개별 저수지에 대해 수행하였고, 탁수 유동 시나리오 해석은 임하호에 심각한 탁수장기화 문제가 발생했던 2002년을 대상으로 댐 연계 탁수모의를 수행하였다. 안동호와 임하호의 댐 앞에서 모의값과 실측값을 오차를 분석한 결과 탁수예측오차는 AME 0.5~24 mg/L, RMSE 0.7~30.2mg/L의 범위로 비교적 실측값을 잘 반영한 것으로 나타났다. 임하댐의 경우 탁수층의 위치와 두께, 그리고 최고 탁도값을 적절히 재현 하였지만, 안동댐은 최고 탁도값 예측에서 다소 오차가 발생하는 것으로 나타났다. 안동호와 임하호 단독 운영시와 연계 운영시의 탁수변화 파악을 위해 초기 홍수사상이 발생한 8월 이후부터 저수지내의 TSS농도 분포를 비교하였다. 안동호의 경우 댐앞지점의 탁수분포는 수온성층구조에 영향을 받아, 단독 운영시(EL. 130 m)보다 연계운영시(EL. 140 m)에 탁수의 중심이 높은 위치에 형성되었다. 단독 운영시 10월 이후에 전도현상으로 인해 침강되지 않은 잔류 탁수층이 저수지 하부로 확산되었지만, 연계 운영시에는 재부상 되어 상층으로 확산되는 것으로 모의되었다. 또한 연계운영시 유량이동으로 인해 안동호의 탁수 댐앞 도달시간이 짧아지는 것으로 나타났다. 반면 임하호는 연계 운영시 안동댐으로 유출이 생기면서 중층에서 탁수량이 저감되는 것으로 모의되었다. 저수지 내 탁수량 분석을 위해 SS 15 mg/L 이상의 잔류 탁수량을 분석한 결과, 연계운영시 안동호의 평균 잔류탁수량 비율은 11.8% 증가, 임하호의 경우 11.7% 감소하였다. 또한, 탁수의 댐하류 방류일수도 SS 15 mg/L 기준 임하호 9일 저감, 안동호는 70일 증가하여 임하호의 탁수가 안동호의 탁수 장기화에 영향을 주는 것으로 나타났다.

• Ecology and Resilient Infrastructure
• /
• v.8 no.2
• /
• pp.88-96
• /
• 2021

In surface image velocimetry, a wide area of a river is photographed at an angle to measure its velocity, inevitably causing image distortion. Although a distorted image can be corrected into an orthogonal image by using 2D projective coordinate transformation and considering reference points on the same plane as the water surface, this method is limited by the uncertainty of changes in the water level in the event of a flood. Therefore, in this study, we developed a tilt image correction technique that corrects distortions in oblique images without resetting the reference points while coping with changes in the water level using the geometric relationship between the coordinates of the reference points set at a high position the camera, and the vertical distance between the water surface and the camera. Furthermore, we developed a distortion correction method to verify the corrected image, wherein we conducted a full-scale river experiment to verify the reference point transformation equation and measure the surface velocity. Based on the verification results, the proposed tilt image correction method was found to be over 97% accurate, whereas the experiment result of the surface velocity differed by approximately 4% as compared to the results calculated using the proposed method, thereby indicating high accuracy. Application of the proposed method to an image-based fixed automatic discharge measurement system can improve the accuracy of discharge measurement in the event of a flood when the water level changes rapidly.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.8 no.4
• /
• pp.520-527
• /
• 2020

Hwangtoh is the rich resource that accounts for about 15.0% of the domestic soil, and can be used as the admixture of concrete with Pozzolan characteristics if activated by rapidly freezing after burning with high temperature. In this study, the mortar curbs containing active hwangtoh were produced, based on the mixture for the mortar curbs sold on the market. The substitution rate of active hwangtoh were considered 10.0% and 25.0%, and the test items were selected to compressive and flexural strength tests, freezing/thawing resistance tests, accelerated carbonation tests, and accelerated chloride diffusion tests. In the results of the mechanical performance, it was showed that the highest strength was evaluated in OPC mixture, and the increase in strength was small by the increase of age, which was believed to be due to the fact that most of the strength in each mixture was created in three days of steam curing. The results of the freezing/thawing tests for 28 aged days showed the reduction rate of compressive strength was 85.0% or higher for all specimen, meeting the criteria presented. The accelerated carbonation tests were carried out on the specimen at 28 days of age, and the results showed that the mortar with active hwangtoh had lower carbonation resistance performance than mortar with OPC. The passed charge of each mixture was assessed in accordance with ASTM C 1202 on 28 and 91 aged days. The OPC mixture had "Low" rate and the mortar with active hwangtoh had "Moderate" rate. So it was thought that the mortar with active hwangtoh had appropriate resistance performance for chloride attack.

• Journal of Korean Society of Disaster and Security
• /
• v.14 no.1
• /
• pp.1-8
• /
• 2021

A corrugated pipe is widely used in firefighting equipment and sprinkler pipes because of its elasticity, which is less damaged by deformation and convenient facilities. However, the corrugated shape of the wall results in complex internal turbulent flow, and it is difficult to predict the pressure drop, which is an important design factor for pipe flow. The pressure drop in the corrugated tube is a function of the shape factors of the pipe wall, such as groove height, length, and pitch. Existing studies have only shown a study of pressure drop due to length changes in the case of D-shaped tubes with less than 5 pitch (P) and height (K) of the rectangular grooves in the tube. In this work, we conduct a numerical study of pressure drop for P/Ks with length and height changes of 2.8, 3.5 and 4.67 with Re Numbers of 55,000, 70,000 and 85,000. The pressure drop in the corrugated tube was interpreted to decrease with smaller P/K. We show that the pressure drop is affected by the change in the groove aspect ratio, and the increase in the height of the groove increases the recirculation area, and the larger the Reynolds number, the greater the pressure drop.

• Journal of Conservation Science
• /
• v.37 no.3
• /
• pp.209-219
• /
• 2021

In this study, the wind environment in an urban area near Sungneymun gate was numerically investigated in the cases of inflow directions. The wind fields for the target area were simulated using Geographic Information System data and Computational Fluid Dynamics model. Results, including vector fields, three-dimensional wind velocity components, and wind speeds, were analyzed to examine flow characteristics. Wind direction variability affected by buildings was shown in the target area. The complex flows around Sungneymun did not depend on the inflow direction as a boundary condition. The wind speed around Sungneymun was generally 3 times stronger at 14 m above ground level (AGL) compared to the surface wind at 2 m AGL and relatively high in the case of easterly inflow. The effect of wind was also analyzed to be relatively significant at the southeast side of Sungneymun. Thus, it was suggested that the assessment of wind environment affected by high-rise and high-density buildings should be necessary for the architectural heritage in urban areas.

• Journal of the Korean Recycled Construction Resources Institute
• /
• v.11 no.1
• /
• pp.89-96
• /
• 2023

In this paper, a ferrosilicon by-product was evaluated to confirm the feasibility of recycling it as supplementary cementitious material of ordinary Portland cement in concrete. Three different levels of replacement ratio (10 %, 20 % and 30 % of total binder) were applied to find which is the most beneficial to be used as a binder. Ferrosilicon concrete was initially assessed at setting time and compressive strength. Durability was evaluated by the resistance to chloride penetration test(RCPT) and alkali-silica reaction(ASR) with a comparison to silica fume concrete due to their similarity in chemical composition. The porosimetry and X-ray diffraction analysis along with energy dispersive X-ray spectroscopy give information on the microstructural characteristics of the ferrosilicon concrete. It was found that 10 % ferrosilicon concrete has higher strength while 20 %, 30 % have lower strength than OPC concrete. However, chemical resistance to chloride attack is higher when replacement is increased. Compared to silica fume, the durability of ferrosilicon might be less efficient however, it is obviously beneficial than OPC. High SiO₂ content in ferrosilicon results in producing more C-S-H gel which could make denser pore structure. Most of the risk of alkali silica reaction to silicate binders through length change tests was less than 0.2 %, and both mortar using ferrosilicon and silica fume showed better resistance to alkali silica reaction as the substitution rate increased.Reuse of industrial waste rather than producing highly refined additives might reduce environmental load during manufacture and save costs.