• Clinical and Experimental Reproductive Medicine
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• v.32 no.2
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• pp.101-111
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• 2005

배 경: Pituitary adenylate cyclase-activating polypeptide (PACAP)은 양의 시상하부에서 추출된 신경펩타이드 호르몬으로 난소에도 존재하여 배양된 과립막 세포에서 스테로이드합성과 cyclic AMP 형성을 촉진함이 보고되었다. 목 적: 흰쥐 난소를 실험 모델로 사용하여 배란시 황체화호르몬 (luteinizing hormone; LH)에 의해 유도된 PACAP과 PACAP 수용체의 유전자 발현양상과 신호 전달경로를 규명하고자 하였다. 재료 및 방법: 미성숙 흰쥐의 배란전 난포를 체외 배양하면서 LH로 처리하고 PACAP 및 PACAP수용체의 유전자 발현을 보기 위해서는 Northern blot 분석과 in situ hybridization (ISH)을, 그리고 단백질 수준의 PACAP 검색을 위해서는 enzyme linked immunosorbent assay (ELISA) 분석을 이용하였다. 결 과: LH 처리 후 Northern blot상의 PACAP 유전자 발현은 6~9시간에 일시적으로 최고치에 도달하였으며 ISH로 보아 과립막 세포에서 발현됨을 알 수 있었다. ELISA 분석 상 PACAP 단백질도 LH처리 후 6~12시간에 최고치를 나타내었으며, PACAP 수용체 mRNA 역시 3~9시간에 최고치로 과립막 세포에서 발현되었다. Adenylate cyclase (AC) 억제제인 MDL12330A 처리시 LH로 발현된 PACAP mRNA가 감소되며, AC의 활성제인 forskolin 처리에는 LH시와 유사한 PACAP mRNA의 발현양상을 나타내었다. 그러나 protein kinase C (PKC)의 억제제인 chelerythrine과 2-0-tetradecanolphorbol-13-acetate (TPA) 처리로는 PACAP 의 유전자 발현에 영향을 주지 못하였다. 5-lipoxygenase의 억제제인 MK886이나 nordihydroguaiaretic acid (NDGA)로 처리한 결과 LH로 유도된 PACAP 유전자의 발현이 감소되었으나, cyclooxygenase의 억제제인 indomethacin은 별로 영향을 주지 못하였다. MEK와 p38의 억제제인 PD98059와 SB203580도 LH로 촉진 된 PACAP의 유전자 발현을 농도 의존적으로 억제하였다. 결 론 : 배란전 난포에서 PACAP과 PACAP 수용체의 유전자 발현은 모두 LH의 폭발적 분비에 의해 유도되어 일시적으로 과립막 세포에서 나타나 배란을 위한 국소적인 조절 작용을 할 것으로 추정되며, LH로 촉진된 PACAP 유전자 발현을 위한 신호전달은 cAMP-PKA, lipoxygenase 및 MAP kinase 경로를 통하는 것으로 사료된다.

• Tribology and Lubricants
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• v.35 no.1
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• pp.77-86
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• 2019

The present study focuses on the effect of staggered integration factor (SIF) on Morton effect simulation results. The Morton effect is a synchronous rotordynamic instability problem caused by the temperature differential across the journal in fluid film bearings. Convection and conduction of heat in the thin film displaces the hot spot, which is the hottest circumferential position in the thin film, from -20 to 40 degrees ahead of the high spot, where the minimum film clearance is experienced. The temperature differential across the journal causes a bending moment and the corresponding thermal bow in the rotating frame acts like a distributed synchronous excitation in the fixed frame. This thermal bow may cause increased vibrations and continued growth of the synchronous orbit into a limit cycle. The SIF is developed assuming that the response of the rotor-lubricant-bearing dynamic system is much quicker than that of the bearing-journal thermal system, and it is defined as the ratio between the simulation time of the thermal system and the rotor-spinning period. The use of the SIF is unavoidable for efficient computing. The value of the SIF is chosen empirically by the software users as a value between 100 and 400. However, the effect of the SIF on Morton effect simulation results has not been investigated. This research produces simulation results with different values of SIF.

• The Journal of Internal Korean Medicine
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• v.40 no.3
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• pp.295-311
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• 2019

Objectives: This research aimed to investigate Chinese clinical studies on the treatment of central post-stroke pain (CPSP) and thalamic syndrome after stroke with traditional herbal medicine (THM). Methods: Randomized controlled trials verifying the effects of herbal medicine on treating CPSP and thalamic syndrome after stroke were included in the study. Electrical and hand search were conducted in the China National Knowledge Infrastructure (CNKI), National Discovery for Science Leaders (NDSL), Research Information Sharing Service (RISS), Oriental Medicine Advanced Searching Integrated System (Oasis) for CPSP and thalamic syndrome after stroke. A literature search was performed in the Chinese and Korean databases for papers published from January 1, 2010 to October 1, 2018. The selected literature was assessed by Cochrane's risk of bias. Results: Twelve reports on randomized controlled trials met the inclusion criteria from the 227 identified reports. Effective rate, comparison of visual analogy scale, present pain intensity, pain grading index, recurrence rate, follow-up, and a 36-item short form survey instrument were used to evaluate the treatments. The effective rate of the treatment group was significantly higher than that of the control group in all papers. Side effects occurred less frequently in the treatment group than in the western medicine control group. Conclusions: The treatment of CPSP and thalamic syndrome after stroke with THM was shown to be highly effective. Additional well-designed clinical trials are needed. This study can be used as a basis for further research on the treatment of CPSP and thalamic syndrome after stroke.

• Journal of Life Science
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• v.29 no.1
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• pp.118-122
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• 2019

Oxytocin (Oxt) and vasopressin (Avp) are mainly synthesized in neuronal cells of the hypothalamus and are released from the posterior pituitary. The structure and sequences of Oxt and Avp genes imply that they are closely related and that they are the result of a duplication event during evolution. A previous study suggested that a small regulatory microRNA (miRNA), miR-24, regulated Oxt after binding. However, it is not clear whether this miRNA can modulate Avp simultaneously. The aim of the present study was to investigate putative targeting miRNAs of Avp, including miR-24. Targeted candidate miRNA oligonucleotides were transfected into COS-7 cells to elucidate the binding activity of miRNAs and Avp using dual-luciferase assays. The luciferase assay showed that only miR-24 displayed elevated binding activity with Avp as compared to a control and other candidate miRNAs. Transfection with seed mutants of Avp and miR-24 inhibitors clearly showed that miR-24 can directly bind to the Avp gene. These results provide new insight into the regulatory mechanism of neurohypophysial hormones by a single miRNA.

• Journal of the Korea Society of Computer and Information
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• v.26 no.6
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• pp.129-135
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• 2021

The purpose of this study was to investigate the relationship of the head posture with the tone and stiffness of the cervical extensor muscles. Eighty adults in their twenties were chosen as subjects, and the tone and stiffness of the cervical extensor muscles were measured, with their usual head posture in the sagittal plane. For the measured head posture, the craniovertebral angle (CVA), craniorotation angle (CRA), and forward shoulder angle (FSA) were analyzed using Image J. It was observed that the tone and stiffness of the upper trapezius muscle increased significantly with a decrease in the CVA as well as with an increase in the CRA (p < 0.05). As a result of further classification into the normal and forward head postures based on the CVA of the subjects, the forward head posture was characterized by a significant increase in the tone and stiffness of the upper trapezius muscle (p<.05). The results of this study are expected to be used as basic data for the evaluation of the forward head posture and posture education in clinical practice.

• Journal of Industrial Convergence
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• v.20 no.4
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• pp.79-84
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• 2022

This study analyzed the relationship between supervision and job commitment of the elderly leisure welfare center employees and the mediating effect of professionalism perception. The survey was conducted of 267 employees in 15 facilities located in Daejeon and Chungnam. The main methods were confirmatory factor analysis and structural equation modeling analysis using SPSS and AMOS. The analysis results showed that the relationship between supervision and job commitment and the mediating effect of professionalism perception were verified. For activating the supervision, it is necessary to strengthen the educational supervision, systematize the supervision, and strengthen the supervisor education. To raise the professionalism perception, programs such as awards for outstanding manpower, social support, and employee education considering clients are needed. It is expected that studies on various variables for human resource management including job commitment for elderly leisure facility employees will be continued.

• Journal of Internet of Things and Convergence
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• v.9 no.3
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• pp.45-53
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• 2023

This study is a nonequivalent placebo control group pretest-posttest design to confirm the effects of auricular pressure therapy on perceived stress and sleep quality for nursing students. The subjects of the study were selected from K College, 30 people in the treatment group and 30 people in the control group, a total of 60 people. Data were collected from March 2 to April 26, 2022. Auricular pressure was applied to the treatment group (shenmen, heart, thalamus), and the placebo control group was applied to the auricle (hip, knee, ankle) in the same method during the same period. Data were analyzed independent t-test repeated measures ANOVA using the SPSS 23.0 program. The perceived stress score of the treatment group significantly decreased to 2.28 after 4 weeks of intervention and 2.07 after 8 weeks of intervention, and the sleep quality score significantly increased to 3.37 after 4 weeks of intervention and 4.02 after 8 weeks of intervention. It was found to have a lasting effect over time.

• Korea Science and Art Forum
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• v.25
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• pp.379-388
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• 2016

There are some studies to get rid of slice patterns in coronal and sagittal images by FFT(Fast Fourier Transform) and Notch filter. However, the use of a notch filter is the ringing effect according to the rapid frequency change occurs in common. Therefore, this study will be to evaluate the shape and effect of the notch filter. In addition, it is proposed a new notch filter design. Results of the study are as follows. First, Emission-Notch filter was relatively naturally correcting the cutting pattern. Second, Emission-Notch filter to use as Ringing effect is reduced relatively. The next through these findings and further research progress more completely removed the Ringing effect, and expects to reproduce the data close to the actual human body information.

• The Journal of the Society of Stroke on Korean Medicine
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• v.19 no.1
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• pp.9-20
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• 2018

■ Objectives The purpose of this case report is to show the effect of Traditional Korean Medicine(TKM) on a patient with insomnia occurred after onset of thalamic hemorrhage. ■ Methods A patient with insomnia diagnosed as thalamic hemorrhage was treated with herbal medication, acupuncture, electro-acupuncture, cupping and moxibustion. Then we evaluated the improvement with the number of times the patient woke up, PSQI-K(Pittsburgh Sleep Quality Index-Korean), ISI-K(Insomnia Severity Index-Korean) and KESS(Korean version of Epworth sleepiness scale). ■ Results Decrease in scores of PSQI-K, ISI-K, KESS and the number of times the patients woke up were observed after the TKM treatment. ■ Conclusion This case showed the effect of TKM treatment on poststroke insomnia.

• Proceedings of the Korea Water Resources Association Conference
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• 2021.06a
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• pp.55-55
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• 2021

지난 2019년 말부터 계속해서 전 세계로 확산되고 있는 COVID-19로 인해, 인간 생활을 둘러싼 사회 및 산업 구조가 다양하게 변모하고 있다. 국내에서는 2020년 2월 발생한 1차 대유행을 기점으로, 2020년 8월 2차 대유행 및 2020년 12월 3차 대유행을 겪었으며, 그 과정에서 마스크 착용 의무화, 개인위생 권장, 사회적 거리두기 등 강력한 예방수칙들이 도입되어 왔다. 특히 COVID-19의 심각성에 따라 단계적으로 발효되는 방역대책 및 사회적 거리두기는 개인뿐만 아니라 상업, 산업 등 대부분의 생활양식을 계속해서 변화시켰으며, 다양한 인프라 시설의 운영 및 관리에 혼란을 야기하고 있다. 세계 각국의 연구기관에서는 이러한 봉쇄령(Lockdown) 정책의 영향으로 물관리 분야에 위기가 닥쳐올 것으로 예견하였으며, 국내의 경우 서울시상수도사업본부 조사에 따르면 가정용수 사용량이 3.4 % 증가하고, 상업 및 공공부문 용수 사용량이 25 %까지 감소하는 등 기존 물 사용 패턴과 상이한 양상을 나타내고 있다. COVID-19 확산이 용수 사용량에 미치는 대표적인 영향은 크게 다음과 같은 세 가지 변화로 구분할 수 있다. 1) 사회적 거리두기 강도에 따른 물 사용량의 급격한 증가와 감소 등 용수 이용규모의 변화, 2) 상업 및 공공부문의 물 수요에서 생활부문 물 수요로 옮겨가는 등 용수 사용처의 변화, 3) 순간 최대 물 사용시간 등 용수 사용패턴의 시간적 변화 등이다. 본 연구에서는 시간별, 수요처별 용수 사용량 등의 상세한 데이터 확보가 가능한 국내 지역을 대상으로, COVID-19의 확산 및 그로 인한 사회적 거리두기의 단계적 시행이 용수 사용량 변화에 미치는 영향을 시공간적으로 분석하였다. 본 연구는 대규모 재난 발생 시 사회적 대응방안에 따른 물 사용량의 변화를 분석함으로써, 현재까지도 지속되고 있는 COVID-19 확산 상황에서 안정적이고 효율적인 상수도 공급방안 마련에 기여하고, 또한 지금까지 물리적 재난 대응에 편중되었던 물 위기 관리 패러다임을 사회적 재난 대응 분야까지 확장시키는 계기를 마련할 것으로 기대된다.