• Applied Microscopy
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• v.18 no.2
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• pp.177-186
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• 1988

Degeneration of the axon terminals of mamillo-thalamic tract following the electrical coagulation of mamillary body is well known. In this study, the author investigated the ultrastructural alterations of neuropil components, initiated by terminal degenerations. Rats weighing approximately 250 gm were fixed on the stereotaxic instrument(David Kopf Inc., Heavy duty model), and NE 300 active electrode(Rhodes Med. Instr. Inc.) was introduced to the mamillary position of anterior 3.8 mm, lateral 0.5 mm, height 3.8 mm and lateral angle of $23^{\circ}$ according to De Groot's Atlas. Electric current of 20 mA was applied during 1 minute between active and inactive electrodes with Radio Frequency Lesion Generator(RFG 4, Radionics Inc.). Two hours, 2 days, 1 week and 2 weeks following the electrical coagulation of mamillary body, ipsilateral anterior thalamic nucleus was fixed in 1% glutaraldehyde-l% paraformaldehyde and 2% osmium tetroxide, embedded in Araldite mixture, cutted with LKB ultra tome V, stained with uranyl acetate-lead citrate and observed with JEOL 100 CX electron microscope. Observed results were as follows; 1. Degenerated mamillo-thalamic synapses were observed to form asymmetric axospinous or axo-dendritic types. 2. Terminal degeneration was not easily discernible at 2 hours interval after mamillary lesion, but following 2 days the terminal degeneration was apparent. 3. Postsynaptic spines, dendrites and even their cell bodies show edematic changes caused by the degeneration of postsynaptic counterpart. 4. Astrocytic territories, including perivascular processes forming glial limitans of blood-brain barrier, exhibit remarkable expansion. 5. Oligoglia and astroglia are actively engaged in the removal of degenerated elements. 6. Active forms of microglia were increased. 7. The observed results may represent typical ultrastructural alteration pattern within neuropil following the degeneration of certain input axon terminals.

• Korean Journal of Animal Reproduction
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• v.20 no.4
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• pp.385-393
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• 1997

다세포 생물에서 몸의 효율적 생존을 위한 각 기관의 homeostasis는 세포 증식과 사망에 의해 조절된다. 따라서, apoptosis라 명명된 세포사망은 정교한 기전에 의한 능동적이고 자발적인 사망기전으로써 몸의 정상적 유지를 위한 필수적인 현상이다. 발생기 세포나 신경세포 또는 흉선세포 분화 동안 과다한 세포의 제거가 apoptosis의 대표적인 예이며, 각종 호르몬에 의해 그 기능이 조절되는 난소세포에서도 apoptosis가 활발히 일어난다. Rat난소에는 태어날 때 수십 만개의 난포를 지니고 있는데, 이 중 단지 1%만이 배란에 사용되어질 뿐이고 나머지는 모두 사망하게 된다. 이러한 난포사망은 난소의 적절한 세포 수를 유지하기 위한 필수적 과정이며, 인위적으로 apoptosis를 억제하는 유전자인 bcl-2를 과다 발현시키면 난소암이 발생하는 연구결과가 이를 입증해주고 있다. 이처럼 중요한 난포 사망기전은 apoptosis라는 개념이 정립되면서 최근 들어 점차 그 연구가 활발해지고 있다. Apoptosis의 특징 중 뚜렷한 점은 DNA가 일정한 간격으로(180∼200 bp)잘려지는 DNA fragmentation현상으로, 이를 이용하여 DNA3'-end 부위에 방사선동위원소를 label한 후 이를 전기영동으로 분리하면 apoptosis를 손쉽게 측정할 수 있다. 난소의 기능은 시상하부호르몬인 LH와 FSH 뿐만 아니라 난소에서 분비되는 각종 난소국부호르몬들에 의해 조절된다. 특정한 발육단계의 난포는 특정한 호르몬에 의해 그 기능을 조절 받는데, 이러한 난소기능 조절기작은 매우 복잡한 경로를 지니고 있다. 이러한 복잡한 기작으로 인해 초기 연구에서첨 생체 내에서 밝히려는 연구 시도는 어려움에 부딪치게 되었다. 생체내 실험은 난소가 다양한 발육단계의 난포를 동시에 지니고 있어 특정한 발육단계의 난포 사망기전을 연구하기 어렵다. 또한 난포는 생체 내에서 다양한 호르몬을 동시에 분비하기 때문에 특정한 난소국부호르몬이 사망기전에 미치는 영향을 조사하기 힘든 점이 있다. 최근 들어 난포체외배양이 다양하게 개발되면서, 이러한 어려운 점을 극복할 수 있게 되었다. 본 논문은 각 발육단계의 난포를 절단해 체외배양하면서, apoptosis DNA 절단 현상을 이용하여 각종 난소국부 호르몬들이 난포발육단계별로 사망기전에 미치는 영향을 요약해 보였다. 난포는 발육하면서 점차 복잡한 호르몬 경로를 생존을 위해 필요로 한다. Prevulatory난포생존에 필요한 난소국부호르몬들은 early antral 단계의 난포에서는 그 미치는 영향이 감소되다가 preantral단계의 난포에서는 영향을 전혀 미치지 못했다. 단지 예외는 cGMP처리로써, 세포내 cGMP수준을 일정하게 유지시켜주는 것이 난포발육단계에 무관하게 생존에 중요한 인자로, 장래 연구는 난포 세포내의 cGMP수준을 조절하는 기작을 규명하는데 있을 것이다.

• Development and Reproduction
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• v.7 no.1
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• pp.1-7
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• 2003

Most animals, including human beings, live in a cyclic pattern of lift that is influenced by the ambient changes of environment. The regular changes occurred by rotation of the Earth itself its revolving around the Sun, and the local environment, are reflected by the distinct behavior in the living organisms. These regular changes of environment have been imprinted into the genes within the living organisms through the evolutionary process over a long period of time. The genes are expressed by rhythms during the process of fetal development followed by growth. The environmental modifications ultimately are settled in genes, serving as a biological clock that is located putatively in the hypothalamus. Thus the biological clock governs a large number of rhythms and affects the time of birth and death lift expectancy, behavior, physiology, cell division, biochemical reaction, etc. The rhythms are readjusted to the changes of environmental cues. The biological clock has the great advantage of predicting and preparing the regular changes of environment.

• Development and Reproduction
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• v.7 no.1
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• pp.35-40
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• 2003

Gonadotropin releasing hormone(GnRH), which is secreted from the hypothalamus, has a pivotal role in the reproduction of mammals. Golden hamsters are seasonal breeding mammal and their sexual activity is determined by photoperiod(length of light per day). Long photoperiod(LP, $\leq$ 12.5 hours of light) maintains the reproductive activity and short photoperiod(SP, $\leq$ 12 hours of light) suppresses it. In order to investigate in detail, the sexual activity was individually examined in SP-housed male golden hamsters received a vector at three different concentrations which contains rat GnRH cDNA. The gonadal regression was significantly(P<0.05) accelerated by the highest concentrations of the vector at 8 and 10 weeks after the treatment in comparison to the other groups. In the light of pulsatile release of GnRH in maintaining reproductive activity, the vector containing GnRH cDNA might secrete the GnRH in a constant high level. These results suggest that the GnRH-containing vector might desensitize the anterior pituitary, leading to acceleration or testicular regression.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.33 no.3
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• pp.218-224
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• 2011

Purpose: The purpose of this study is to evaluate the post-operative skeletal stability after surgical correction of patients with mandibular prognathism by bilateral sagittal split ramus osteotomy (BSSRO) and to evaluate the horizontal relapse tendency after the surgery. Methods: Twenty-six patients with Class III dental and skeletal malocclusion were selected for this retrospective study. Fifteen of them underwent BSSRO for mandibular setback and eleven of them underwent two-jaw surgery (Lefort I and BSSRO). In each patient, lateral cephalometric radiographs were taken pre-operatively, post-operatively within 1 week, and post-operatively after eight months. After tracing of the cephalometric radiographs, various parameters were measured. The analyses were done by linear measurement to evaluate the change in position of hard tissue B point, pogonion and mandibular plan angle by examination on lateral cephalograms. Results: The horizontal relapse rate was 27.1% at B point and 31.6% at pogonion in patients who underwent BSSRO. The horizontal relapse rate of the group where the amount of correction exceeded 10 mm was 25.69% at B point. Conclusion: There were no statistical differences on the magnitude of setback and direction of rotation of the mandible in mandibular stability. There were also no statistical differences between single mandibular surgery and two-jaw surgery for mandibular stability.

• Physical Therapy Korea
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• v.18 no.3
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• pp.26-37
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• 2011

Although there have been various studies related to the body's movement from a sitting to a standing position (sit-to-stand task), there is limited information on the kinematic changes on the frontal and transverse planes. The purpose of this study was to ascertain how pelvic tilt affects kinematic changes in the frontal and transverse planes in the hip and knee joints during a sit-to-stand task. For this study, 33 healthy participants (13 female) were recruited. Each participant rose from a sitting to a standing posture at his or her preferred speed for each of three different pelvic tilt trials (anterior, posterior, and neutral), and the measured angles were analyzed using a 3-D motion analysis system. A one-way repeated measure analysis of variance was performed with Bonferroni's post hoc test. In addition, an independent t-test was carried out to determine the sex differences in hip and knee joint kinematic changes during the sit-to-stand tasks. The results were as follows: 1) The hip and knee joint angle in the frontal and transverse planes showed a significant difference between the different pelvic tilt postures during sitting in the pre-buttock lift-off phase (pre-LO) (p<.05). Compared to the posterior pelvic tilt posture, the anterior pelvic tilt posture involved significantly greater hip joint adduction and internal rotation, knee joint adduction, and reduced internal rotation of the knee joint. 2) Sex differences were found with significant differences for males in the initial and maximal angles in the frontal plane of the hip and knee joint (p<.05). Females had a significantly smaller initial abduction angle of the hip joint and a significantly greater maximal angle of the hip adduction joint. These results suggest that selecting a sit-to-stand exercise for pelvic tilt posture should be considered to control abnormal movement in the lower extremities.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.37 no.12
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• pp.1121-1127
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• 2013

This study deals with experimental studies on electromyogram (EMG) measurements and functional electrical stimulation (FES) for the rehabilitation of a shoulder-joint. Based on the structure, motion, and main functions of the musculoskeletal system in a shoulder-joint, the muscles playing a major role for the motion in the sagittal plane were selected for the experiment. First, the surface electromyogram of the main muscles was measured according to the joint angle. The results showed that the change in the surface EMG was linearly proportional to the change in the joint angle. Second, the joint angle was measured during FES at shoulder muscles. The results showed that the joint angle increased as the FES current increased in a certain range of FES. It was confirmed that the willingness of muscles to move could be detected by measuring EMG and that the generation of muscle tension could be assisted by FES for active rehabilitation.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.30 no.5
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• pp.359-367
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• 2004

Purpose: After the surgical correction with sagittal split ramus osteotomy, the position of the mandibular condyle in the glenoid fossa and the proximal segment of the mandible change because of bony gap between proximal and distal segment, especially in case of mandibular setback asymmetrically. In this study, positional changes in the condyle and proximal segment after BSSRO were estimated in the mandibular asymmetry patient by analyzing the in submentovertex view and P-A cephalogram for identification of ideal condylar position during surgery. Patients and Methods: The 20 patients were selected randomly who visit Dankook Dental Hospital for mandibular asymmetry. Bilateral sagittal split ramus osteotomy with rigid fixation was performed and P-A cephalogram and submentovertex view was taken at the time of preoperative, immediate postoperative, 3 month postoperative period. Results: Intercondylar length and transverse condylar angle was increased due to inward rotation of proximal segment and anteromedial rotation of lateral pole of condyle head. The condylar position had a tendency to return to the preoperative state and after 3 months return up to about half of the immediate post-operative changes, and all the results showed more changes in asymmetry patient and deviated part of the mandible. Conclusion: Based on all these results above, surgeon should make efforts to have a precise preoperative analysis and to have a ideal condylar position during rigid fixation after BSSRO.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.5
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• pp.537-542
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• 2008

BSSRO is most frequently operated among orthognathic surgery techniques for repairment of maxillofacial deformities. In case of patients with maxillofacial asymmetry accompanying mandibular protrusion who are operated by BSSRO, this study considers the recovering time for masticatory force of each tooth and Masseteric EMG and the adequate time enabling normal occlusion. The patients who are operated with BSSRO under general anesthesia in Dankook Dental Hospital, Department of OMS are selected for this study. The control group is devided into 2. 26 patients with facial asymmetry accompanying mandibular protrusion are selected for group 1 and their maximum voluntary bite force and masseteric EMG are measured. Group 2 is formed by volunteers with healthy dentition who are measured maximum bite force and masseteric EMG on both sides of the mouth. At the week of 3rd, 5th, 7th, 9th and 11th, Mann-Whitney U test is carried on for statistical analysis and the result is as follows. 1. Patients with mandibular protrusion showed apparently low maximum bite force and masseteric EMG than patients with normal occlusion. 2. In comparison with control group 1, Occlusal force is regained in incisors and canines at the 9th week and in premolars and molars, 11th week and masseteric EMG is regained at 11th week. 3. Comparing to normal occlusal patients, no recovery could be found in experimental group in every parts of the mouth.

• Maxillofacial Plastic and Reconstructive Surgery
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• v.29 no.2
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• pp.187-191
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• 2007

An ameloblastoma is one of the most common odontogenic tumors. Ameloblastoma is cytologically a benign tumor, but is clinically characterized by infiltrative growth and high recurrency. The treatment of ameloblastoma has been controversial. The aim of this paper is to consider effectiveness of curettage and enucleation after SSRO in the small-sized multilocular intraosseous ameloblastomas that have been treated more frequently by radical treatment. They were radiographically characterized by the cortical bone that was expanded or eroded locally and histopathologically by solid multilocular ameloblastomas. It is considered that curettage and enucleation after SSRO and long-term follow-up enable the small-sized multilocular intraosseous ameloblastomas that were characterized by almost destroyed cancellous bone and expanded cortical bone to treat minimizing facial disfigurement and masticatory dysfunction and sociopsychological impact produced by radical treatment. We recommend that the small-sized multilocular intraosseous ameloblastomas without involvement to the surrounding soft tissues be first treated by curettage and enucleation after SSRO.