• The Korean Journal of Mycology
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• v.45 no.4
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• pp.259-269
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• 2017

In order to investigate the diversity of yeasts from major rivers (the Daejeoncheon, Gapcheon and Yudeungcheon) located in Daejeon city, we isolated wild yeasts by plating diluents of samples collected during the summer and winter of 2016 onto yeast extract-peptone-dextrose (YPD) medium, then identified them using Basic Local Alignment Search Tool (BLAST) analysis to compare the nucleotide sequences of the PCR amplicons for the D1/D2 domain of 26S rDNA. In total, we isolated 191 yeast strains belonging to 104 species from 148 soil or water samples from the rivers and their junctions. Candida spp. (45 strains) including Candida tropicalis (22 strains) were the most abundantly isolated strains from the Daejeoncheon. Candida spp. (16 strains) including Candida vartiovaarae (8 strains) and Candida spp. (18 strains) such as Candida sake (4 strains) were also the dominant isolates from the Gapcheon and Yudeungcheon, respectively. In conclusion, Candida spp. and Cryptococcus spp. were the most dominant strains, corresponding to 42% and 7% of the 191 yeast strains isolated in this study, respectively.

• Analytical Science and Technology
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• v.18 no.3
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• pp.216-223
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• 2005

In this study, several kinds of volatile amines in ambiant air were collected and their concentrations were expected from the calibration curves prepared by standard solutions of 7 amines by the official measuring method prescribed in Japanese Offensive Odor Law. The obtained calibration curves showed a good linearity and the detection limit of trimethyl amine was found to be about 0.033 ppb (0.040 ng) in case of 50 liters air. It means that trimethyl amine could be detected with the concentration of lower than its permitting level at the border line of companies. As typical examples of measuring amines in air, results of investigation of two sewage works and one rendering plant around the capital area indicated that 4 kinds of amines, i.e. methyl amine, dimethyl amine, trimethyl amine and isopropyl amine were detectable and concentrations of trimethyl amine at the primary sedimentation pond and sludge dewatering building of the sewage plant A showed 9.07 ppb and 7.79 ppb respectively, being over the concentration of odor strength 2.5, And the aeration tank, excrement input facility and indoor of maintenance room of excrement process building in the sewage plant B showed 70.0 ppb of dimethyl amine and 2.44 ppb of trimethyl amine.

• Journal of The Korean Society of Agricultural Engineers
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• v.52 no.6
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• pp.57-62
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• 2010

본 연구는 자유수면형 인공습지에서의 시기적인 중금속 제거율의 변동을 평가하기 위하여 수행되었다. 대상 인공습지의 유입수는 하수처리수가 포함된 하천수이다. 인공습지에는 갈대 (Phragmites australis)와 부들 (Typha latifolia)이 주로 분포하며, 이들 두 종은 습지의 95 % 이상을 차지하는 우점종이다. 2009년 5월부터 9월까지 습지의 유입과 유출부분에서 유입수와 유출수를 시료 채취하였다. 채취된 시료는 ICP-AES 방법을 이용하여 6가지 중금속을 분석하였으며, 위해성 평가를 이용하여 카드뮴 (Cd), 크롬 ($Cr^{+6}$), 비소 (As), 납 (Pb), 니켈 (Ni), 구리 (Cu)의 분석 결과를 평가하였다. 위해성평가는 농부와 레크리에이션에 대한 두가지 시나리오를 바탕으로 이루어졌다. 연평균 중금속 제거율은 계절적인 변화 측면에서 큰 변화를 나타내지 않았다. 위해성 평가 결과 시료가 채취된 부분 및 계절적인 변화 대부분에서 US EPA의 기준인 $10^{-4}$를 초과하지 않는 수준으로 허용가능한 수준이었다. 하지만, 봄철 농부에 대한 위해성평가 결과 위해도가 $10^{-4}$ 수준이며, $10^{-6}-10^{-8}$ 수준은 질병의 이동이 우려되는 수준 (US EPA)임을 감안하여, 인공습지에 중금속 농도 저감 시설의 설치를 고려해야 한다. 본 연구에서는 위해성 평가를 가상의 시나리오에 적용하여 평가를 시도함으로서, 수치적인 자료로 제시할 수 있을 뿐만 아니라 중금속의 정량적인 평가를 수행할 수 있었다. 따라서 위해성 평가는 인공습지의 안전성에 대하여 대중과 소통할 수 있는 도구로서 이용할 수 있으며, 인공습지의 효율적인 운영 및 현장 적용에 있어서 스크리닝 도구로서의 역할이 가능할 것으로 판단된다.

• Proceedings of the Korean Radioactive Waste Society Conference
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• 2005.06a
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• pp.151-157
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• 2005

For the treatment of regulatory clearance of the soils, a procedure for the radionuclides and radioactivity concentration analysis is under development. A strategy for soil sampling including random sampling after homogenization and standardization was set up. Statistical representativeness is considered for not only sampling strategy but also sample size. In this study, designed sample size was designed with confidence interval and error bound of soil using the pilot samples which were taken following the sampling strategy.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.8 no.2
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• pp.91-97
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• 2010

As nuclear power plants are getting older, interests on a decontaminating process are increasingly attracting more attention. Chemical decontamination is crucial to lower the production of radioactive waste and radiation dose rate. Prior to this, oxidizers and detergents for target material should be chosen so as to decontaminate major systems and components of a nuclear power plant chemically. In order to decontaminate it properly, it is crucial to have information about the chemical composition and crystalline structure of CRUD, analyzing its samples from the target or the decontamination system with components. However, there is no program which enables the extraction of samples directly from the object or the decontamination system with components carrying genuine radioactivity. Therefore, it is limited to samples from corrosion products carrying partial radioactivity as a resource. The composition of CRUD varies considerably depending on refueling cycle because it is closely related to the constituent of basic material. After settling a target, it is crucial to analyze and obtain analytical information about CRUD as a decontamination target. In this paper, various technologies for manufacturing simulated CRUD are introduced as alternatives to unattained samples. A metal oxide or metal hydroxide was used to synthesize simulated cruds having chemical compositions and crystalline stricture similar to the actual one by 12 different methods. CRUD 4(metal oxides in the autoclave vessel) and CRUD 10(metal oxides in a crucible after hydrazing pretreatment)were chosen as the best method for Type 1 and Type 2.respectively. As these CRUD can be synthesized easily without using any specialized equipment or reagents in a short time and in large quantities, they are expected to stimulate the development of decontaminating agents and processes.

• Journal of the Korean Geotechnical Society
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• v.17 no.4
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• pp.259-267
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• 2001

실내시험으로 구한 점토의 공학적 성질은 샘플링, 운반, 저장, 그리고 성형과정 동안에 발생하는 시료의 교란으로 인해 원지반의 성질과 다르게 측정된다. 본 연구에서는 양산점토에 대한 삼축압축시험($CK_{o}$ UC) 결과를 이용하여 샘플링 방법에 따른 교란의 정도를 평가하였다. 실험에 사용된 시료는 76mm 튜브샘플러, 76mm 피스톤샘플러, 블록샘플러로 채취되었으며, 시료의 교란정도를 평가하기 위해 각 시료에서 측정된 체적변형률, 비배수 전단강도, Secant Youngs modulus, 그리고 파괴시 간극수압계수를 비교하였다. 시료의 교란정도를 평가하는 것 이외에도 SHANSEP 방법을 이용하여 수행한 $CK_{o}$ U 삼축압축시험 결과를 이용하여 양산점토에 대한 정규화 전단강도($C_{u}$ /$\sigma$$_{vc}$ )와 OCR 관계를 규명하였다. 또, 피에조콘 관입시험, 딜라토메타 시험, 그리고 현장 베인시험결과를 이용하여 구한 양산점토의 비배수 전단강도를 삼축압축시험 결과와 비교하였다.

• Journal of the Korean GEO-environmental Society
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• v.7 no.2
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• pp.55-66
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• 2006

The existing Highway LA-1 of US is required to be replaced for covering increasing regional demands of transportation such as Hurricane evacuation and oil industry. This 28 km crosses wetlands and is a sensitive environmental area. Huge amount of soil investigation and laboratory tests were performed with best efforts to overcome inherit errors of sampling, disturbance, and testing procedures for this project. The data scattering was corrected through using central tendency theory.

• Economic and Environmental Geology
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• v.45 no.2
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• pp.169-180
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• 2012

The ESBs (Environmental Specimen Banks) have monitoring functions for the contemporary environmental qualities and also offer the future generation tangible information on the past environment by preserving the specimens. This entails the sampling of the representative specimen for each distinctive ecosystem, which is performed under a strict and stipulated procedure and a condition that does not allow any change in the component so that a retrospective analysis can be readily done even in the distant future. It has been more than 30 years that some developing countries started collecting a broad spectrum of specimens to vindicate the effectiveness of an environmental policy and to monitor the long-term variations of background concentrations of environmental pollutants. Though being late, the National Institute of Environmental Research (NIER), Korea, has successfully launched the National Environmental Specimen Bank (NESB) in 2009 equipped with its state-of-the-art automated cryogenic tanks. Since then, the researchers at the NESB have been doing their best to excel the existing ESBs around the world by learning and improving the expertise. To do so, they conducted a pilot study for developing and testing their own Standard Operating Procedure (SOP) based on the analysis of the examples of the other ESBs. The problems from the pilot study had been reviewed to improve the SOP to meet the requirements for an ESB, that is to say, preserving representative environmental specimens in cryogenic condition and enhancing the analytical method. Furthermore, they also need to prepare themselves to address the future challenges by providing some additional functions, which makes it distinguishable from the other ESBs. If successful, this will be a step further to be recognized as a full-fledged member of the ESB society of the world.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.5
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• pp.443-454
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• 2010

Phthalate compounds are widely used as plasticizers in polyvinyl chlororide (PVC) resins and other industrial consumer products, and some of them are known to be endocrine disruptors. In Korea, a number of studies have been carried out for the measurement of phthalates in consumer products and drinking water. However, no data are available for those compounds in the ambient air where the general public are routinely exposed. In this study, we evaluated sampling and analytical methods for the determination of phthalates in the ambient atmosphere. A wide range of phthalates compounds were included in the target analytes, which are dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), butyl benzyl phthalate (BBP), di(2-ethylhexyl) phthalate (DEHP), and di-n-octyl phthalate (DOP). Most of samples were collected using a high volume sampler with a PUF/XAD-2 column/quartz fiber filter and then analyzed by GC/MS. Some of samples were simultaneously collected on XAD-2 using a low-volume sampler, together with high-volume samples. The analytical method applied in this study showed good repeatability and linearity. Quantitative detection limits were estimated from 0.60 to 17.84 ng/$m^3$ in air, depending on individual compounds. The field measurements were carried out at 3 sites located in Sihwa- Banwall industrial areas and a suburban area from January 2007 to November 2007. From the field experiments, DEHP, DMP and DBP appeared to be the most abundant compounds in the ambient air. It was also found that DMP, DEP and DBP were mainly distributed in the vapor phase, while BBP, DEHP and DOP were predominantly associated with the particulate phase. The concentrations of DEHP and DMP in the industrial areas ranged from 45.7 to 1,012.7 ng/$m^3$ and from 7.7 to 375.1 ng/$m^3$, respectively. Overall, the high-volume sampling method was demonstrated to be superior to the low-volume method for the determination of phthalates in the ambient atmosphere.

• Journal of Korean Society of Environmental Engineers
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• v.32 no.5
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• pp.455-460
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• 2010

This study was carried out to find out any inherent problems occurring in the sampling and analytical procedures, and to suggest the relevant solutions to the problems. In addition, an optimal condition of clean-up process was developed, which was based on a method using silica glass column. As a result of experiments to test any artificial contamination of blank samples such as glassware and collection media, artifacts of DBP and DEHP appeared to be detected in various kinds of laboratory tools and apparatuses used in the sampling and analytical works. Therefore, it is necessary to investigate a degree of contamination before laboratory works by conducting a prior check any possible contaminations in all experimental tools and apparatus. It is also necessary to devise a method to avoid a tool, if possible, or to use a substitute of phthalate free. If the use of any plastic tool to cause contamination is inevitable, it should be properly corrected with a blank level, as is equally treated as the sample. The clean-up process demonstrated in this study can give us a significant benefit in terms of the quantity and quality of a target compound by GC/MS analysis.