• Title/Summary/Keyword: 시간영역평균법

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흑염소의 체내수정란 생산에 관한 연구

  • 최창용;조숙현;한만희;권응기;최성복;최연호;최순호;손동수;최상용
    • Proceedings of the Korean Society of Embryo Transfer Conference
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    • 2002.11a
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    • pp.86-86
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    • 2002
  • 면양과 염소가 최근 수십년동안 세계여러 나라에서 번식생리의 연구를 위한 모델로 사용되어 왔는데, 체내수정란의 생산에 관한 영역도 유럽을 중심으로 활발하게 연구되어왔다. 수정란생산을 위한 발정동기화방법, 과배란처리 및 수정란회수방법 기술은 현재 상당히 많은 기술진척이 이루어진 상태이나, 우리나라 고유의 재래유전자원인 흑염소에는 이를 위한 기술이 미진한 실정이므로 본 실험에서는 흑염소의 체내수정란생산기술을 확립하여 재래가축 유전자원보존을 위한 기초기술을 마련하고자 한다. 축산기술연구소 남원지소에서 사육하고 있는 체중 20kg 이상의 건강한 흑염소를 이용하여 발정동기화를 위해 controlled intravaginal drug release(CIDR)를 질내에 14일 동안 삽입하고, 과배란처리는 FSH를 CIDR 삽입 12, 13, 14일째에 12시간 간격으로 점감법으로 총20mg을 투여하였으며, PGF$_2$a를 13일째 FSH와 함께 투여하였다. CIDR는 14일째의 아침에 제거하였다. 수컷과의 교미는 CIDR제거 24시간후에 GnRH를 투여와 동시에 실시하였으며, 채란은 교미후 3일째에 외과적인 방법으로 실시하였다. CIDR처리경과에 따른 progesterone농도는 CIDR 주입시 바로 수치가 상승하여 제거전까지 6~12ng/m1의 농도를 유지하였으며, 제거즉시 2ng/ml 이하로 떨어졌다. 채란시 평균 배란점은 16.5개, 미배란난포 9.8개였으며, 회수수정란은 6.0개를 나타내어 채란율은 36.4%를 나타내었다. 회수된 수정란의 발달단계는 4-cell 78.9%, 2-cell 5.3%, fragmentation 15.8%를 나타내었다. 이와 같은 체내수정란생산방법을 기반으로 하여 이후 수정란의 동결 및 수정란이식기법에 관한 연구를 수행한다면 우리나라의 재래가축인 흑염소의 유전자원 장기보존과 생산성향상에 기여할 것으로 사료된다.배양액에 30 embryos/50ul 소적으로하여 38.8$^{\circ}C$, 5% $CO_2$의 탄산가스 배양기에서 각각 7일간 배양을 실시하였다. 조사된 결과는 SAS/STAT를 이용하여 통계분석을 실시하였다. 체외수정 12시간 후에 난자 급속 염색법으로 염색을 실시한 결과, 모든 처리구에서 핵성숙률(76.4~95.2%), 정자침투율(51.1~66.9%), 웅성전핵형성률(95.2~100%), 다정자침입률(18.2~25.6%) 및 평균침입정자수(1.2~l.4개)에서 유의적인 차이가 인정되지 않았다. 체외배양 48시간 난할률을 조사한 결과, 처리구별 차이(53.9~67.9%)는 인정되지 않았으나, 배양 7일째 배반포형성률은 각각 14.5, 25.4, 17.3 및 12.4%로서 25uM의 $\beta$-ME처리구가 유의적(P<0.05)으로 높은 배발달률을 나타내었고, 총세포수에 있어서는 대조구와 처리구간 유의적인 차이가 인정되지 않았다. 따라서 돼지 난포란을 성숙배양할 때, 25uM $\beta$-ME를 첨가배양하는 것이 양질의 돼지체외수정란을 생산하는 하나의 방법으로 조사되었다.다.natural objects and was popular at the time of Yukjo Dynasty, and there are some documents of that period left both in Japan and Korea. "Hyojedo" in Korea is supposed to have been influenced by the letter design. Asite- is also considered to have been "Japanese Letter Jobcheso." Therefore, the purpose of this study is to look into the origin

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Development of Self-Managed Food Sanitation Check-List and On-Site Monitoring of Food Sanitation Management Practices in Restaurants for Control of Foodborne Illness Risk Factors (식중독 발생 위험요인 관리를 위한 외식업체 자가위생관리점검표 개발 및 현장모니터링)

  • Chung, Min-Jae;Choi, Jung-Hwa;Ryu, Kyung;Kwak, Tong-Kyung
    • Korean journal of food and cookery science
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    • v.26 no.5
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    • pp.603-616
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    • 2010
  • Based on recent dramatic increases in foodborne outbreaks in restaurants, self-managed sanitation systems are now recommended to control contributing risk factors. This study aimed to improve sanitation management practices in restaurants and had two objectives. First, we tried to develop a self-managed sanitation check-list, including risk factors contributing to foodborne illness and Korean food hygiene regulation articles. We also tried to evaluate current sanitation management practices in restaurants according to operation and restaurant type. Thirty restaurants were evaluated by on-the-spot inspectors using an auditing tool consisting of four dimensions, seventeen categories, and forty-one items. Total compliance rate categorized by operation type significantly differed between chain restaurants and self-managed restaurants, with values of 85.5% and 51.6%, respectively. Therefore, self-managed restaurants, which showed the lowest compliance rate of below 30.0%, need more strict control to improve current unsanitary management practices, specifically relating to 'sterilization of knives, chopping boards, and wiping cloths', 'sanitation training', 'not allowing access into the kitchen to outsiders', 'handling of food or utensils on shelves at a 15 cm distance away from floor', 'prevention of cross-contamination of cooked foods or vegetables', and 'records of kitchen access or inspection'. Thus, an effective food sanitation system is essential and should be implemented to improve the existing sanitary conditions in restaurants. However, the most important factor to achieving food sanitation management objectives is food handlers' self-motivation.

Simulation Studies on Asymptotic Approximations Analysis of M/M/s and M/D/s Queues (M/M/s와 M/D/s 대기행렬의 점근 근사법 분석을 위한 시뮬레이션 연구)

  • Jinho Lee
    • Journal of Service Research and Studies
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    • v.14 no.3
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    • pp.172-187
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    • 2024
  • This paper deals with asymptotic approximations analysis of M/M/s and M/D/s queues. For M/M/s queue, we observe "economies of scale" under the fixed utilization ρ and the fixed probability α that customer waits in system, how the average system size vary according to the number of servers s increasing. Simulation results show that as s increases, the number of servers who are idling increases, that is, the slack n-E[Qn] diverges. In addition, through changing the waiting probability α under the M/M/s system, α was not highly sensitive to the behavior of the system size. And, it is shown that using ${\rho}_n\,=\,1-k/\sqrt{n}$ to handle heavy-traffic regime is only appropriate for k = 1 by observing the effect on the performance of the system with different values of k. For the M/D/s queue, two approximations are used to evaluate the expected system size under the fixed ρ and α. Simulations and comparison of these two approximations show that Cosmetatos' approximation performs quite well when the number of servers is small and traffic intensity is heavy, but it overestimates the true value for the large number of servers. Meanwhile, the modified approximation gives good results for the steady state count of the system although the number of servers grows large.

A Stable MOT Scheme with Combined Field Integral Equation for the Analysis of Transient Scattering from Conducting Structure (도체 구조물의 과도 산란 해석을 위한 결합 적분방정식의 안정된 MOT 기법)

  • Lee, Chang-Hwa;An, Ok-Kyu;Kwon, Woo-Hyen;Jung, Baek-Ho
    • The Journal of Korean Institute of Electromagnetic Engineering and Science
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    • v.19 no.4
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    • pp.427-435
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    • 2008
  • In this paper, a stable marching-on in time(MOT) method with a time domain combined field integral equation(CFIE) is presented to obtain the transient scattering response from arbitrarily shaped three-dimensional conducting bodies. This formulation is based on a linear combination of the time domain electric field integral equation(EFIE) with the magnetic field integral equation(MFIE). The time derivatives in the EFIE and MFIE are approximated using a central finite difference scheme and other terms are averaged over time. This time domain CFIE approach produces results that are accurate and stable when solving for transient scattering responses from conducting objects. Numerical results with the proposed MOT scheme are presented and compared with those obtained from the conventional method and the inverse discrete Fourier transform(IDFT) of the frequency domain CFIE solution.

Color Image Coding using Variable Block of Fractal (프랙탈 기반의 가변블록을 이용한 컬러영상 부호화)

  • Park, Jae-Hong;Park, Cheol-Woo
    • Journal of the Korean Society of Radiology
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    • v.8 no.7
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    • pp.435-441
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    • 2014
  • This paper suggests techniques to enhance coding time which is a problem in traditional fractal compression and to improve fidelity of reconstructed images by determining fractal coefficient through adaptive selection of block approximation formula. First, to reduce coding time, we construct a linear list of domain blocks of which characteristics is given by their luminance and variance and then we control block searching time according to the first permissible threshold value. Next, when employing three-level block partition, if a range block of minimum partition level cannot find a domain block which has a satisfying approximation error, There applied to 24-bpp color image compression and image techniques. The result did not occur a loss in the image quality of the image when using the encoding method, such as almost to the color in the RGB image compression rate and image quality, such as gray-level images and showed good.

Analysis of Flow Visualization Results Using Fourier Transform (퓨리에 변환을 활용한 유동 가시화 비교 분석)

  • Koo, Bon-guk;Park, Jun-mo;Kang, Yong-Duck
    • Journal of the Institute of Convergence Signal Processing
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    • v.20 no.4
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    • pp.199-204
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    • 2019
  • In the boundary layer, vortical system has been analyzed by the statistical methods to identify the vortex interaction. However, there are the limitations in explaining vortices by the mean velocity or the standard deviation. This paper proposed a method to establish a frequency analysis by Fourier transform in order to simultaneously investigate various scale vortices. For this purpose, the flow visualization conducted to reveal a standing vortex, a hairpin vortex and a wake region around a hemisphere attached on a flat plate in a water channel. In addition, the velocity where the hairpin vortex was being generated in the wake region was measured by a hot-film anemometer. To observe changes in the vortex interaction, suction was applied through a hole in front of the hemisphere. For the evaluation of the proposed frequency analysis, the existing statistical results were compared to the frequency analysis that corresponds to the qualitative results of the flow visualization.

Video Signature using Spatio-Temporal Information for Video Copy Detection (동영상 복사본 검출을 위한 시공간 정보를 이용한 동영상 서명 - 동심원 구획 기반 서술자를 이용한 동영상 복사본 검출 기술)

  • Cho, Ik-Hwan;Oh, Weon-Geun;Jeong, Dong-Seok
    • 한국HCI학회:학술대회논문집
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    • 2008.02a
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    • pp.607-611
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    • 2008
  • This paper proposes new video signature using spatio-temporal information for copy detection. The proposed video copy detection method is based on concentric circle partitioning method for each key frame. Firstly, key frames are extracted from whole video using temporal bilinear interpolation periodically and each frame is partitioned as a shape of concentric circle. For the partitioned sub-regions, 4 feature distributions of average intensity, its difference, symmetric difference and circular difference distributions are obtained by using the relation between the sub-regions. Finally these feature distributions are converted into binary signature by using simple hash function and merged together. For the proposed video signature, the similarity distance is calculated by simple Hamming distance so that its matching speed is very fast. From experiment results, the proposed method shows high detection success ratio of average 97.4% for various modifications. Therefore it is expected that the proposed method can be utilized for video copy detection widely.

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Ultra-thin aluminum thin films deposited by DC magnetron sputtering for the applications in flexible transparent electrodes (스퍼터링법으로 증착된 초박형 Al 박막의 투명전극 적용성 연구)

  • Kim, Dae-Gyun;Choe, Du-Ho
    • Proceedings of the Korean Institute of Surface Engineering Conference
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    • 2018.06a
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    • pp.82-82
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    • 2018
  • 광전소자용 투명전극으로 적용하기 위한 초박형 Al 박막에 대해서 기초연구를 수행하였다. 증착 전 챔버(chamber) 내 기저압력은 $3{\times}10^{-7}Torr$이하로 유지하였으며 Ar 불활성 기체의 유입을 통해 작업압력을 $1{\times}10^{-2}Torr$로 상승시켜 증착을 실시하였다. DC 마그네트론 스퍼터링법을 이용하여 유리기판상에 Al 박막의 증착을 실시하였으며, 박막의 두께가 3-12 nm인 Al 박막을 각각 형성하였다. 두께가 7 nm 일 때 면저항은 $135{\Omega}/{\square}$로 측정되었고 7 nm 이상인 두께의 박막은 두께가 증가할 때 면저항이 점진적으로 감소되는 경향을 확인할 수 있었다. 두께가 10 nm인 박막의 측정된 면저항은 $13.1{\Omega}/{\square}$로 두께 7 nm인 박막과 비교하였을 때 약 10배의 차이를 확인할 수 있었다. 두께 6 nm 이하인 박막은 면저항 측정이 불가능하였는데 이는 SEM 분석 결과, 연속박막을 이루지 못 하였기 때문이라고 결론을 내릴 수 있었으며, 두께 12 nm인 박막까지 완전한 연속박막이 형성되지 않았다. 각각의 박막에서 입자의 크기는 선 교차법(line intercept method)을 이용하여 시편당 평균 120개의 입자에 대한 평균값을 측정하였으며, 이론적으로 예상할 수 있는 바와 같이 두께가 증가할수록 입자크기도 비례하여 증가하게 되는 것을 확인할 수 있었다. 가시광선 파장영역 내 투과도의 경우, 3 nm 두께에서 평균 80% 이상의 투과도가 측정된 데 반하여, 4-5 nm 두께에서 평균 60%로 급격하게 감소되기 시작하며 그 이후, 두께 증가에 따라 투과도가 점진적으로 감소되는 경향을 확인할 수 있었다. 또한 Al 박막은 시간의 경과에 따른 표면의 산화가 진행되어 기존에 측정된 면저항보다 10-60%의 면저항이 증가하였는데 이는 두께가 얇을수록 더 산화의 영향을 많이 받기 때문에 나타난 결과로 보인다. 추후 산화방지막 및 빛반사방지막 층을 초박형 Al박막과 함께 Oxide/Metal/Oxide 구조로 형성하여 위와 같은 현상들을 해결하고 박막물성의 증진을 통해 투명전극에 적용을 목표로 한다.

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Spectral Analysis of Hidden EEG Arousal Activity in Periodic Leg Movements in Sleep without Microarousal (미세각성이 없는 수면중 주기성 사지운동증 뇌파의 스펙트럼 분석)

  • Cyn, Jae-Gong;Seo, Wan-Seok;Oh, Jung-Su;Jeong, Do-Un
    • Sleep Medicine and Psychophysiology
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    • v.10 no.2
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    • pp.100-107
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    • 2003
  • Objectives: Periodic leg movements in sleep (PLMS) might be subdivided based upon whether or not they are associated with visible EEG microarousals (MA). MA is considered to be responsible for nonrestorative sleep and daytime fatigue. The American Sleep Disorders Association's (ASDA) scoring rules for MA based on visual analysis of the EEG changes suggest that MA should last more than 3 seconds. However, it has been suggested that visual analysis may not detect some changes in EEG activity. This study is aimed at measuring changes in EEG spectra during PLMS without MA in order to better understand the arousing response of PLMS. Methods: Ten drug-free patients (three men and seven women) diagnosed with PLMS by polysomnography were studied. Spectral analysis of the EEG was performed in each patient on 30 episodes of PLMS without MA, chosen randomly across the night in stage 2 non-REM sleep. We applied stricter criteria for MA compared to ASDA, by defining it as a return to alpha and theta frequency lasting at least 1 second. Results: The mean PLMS index was $16.7{\pm}10.0$. The mean PLMS duration was $1.3{\pm}0.7$ seconds. Comparison of 4-second EEG activity both before and after the onset of PLMS without MA using independent t-test showed that the movements were associated with significant increase of relative activity in the delta band (p=0.000) and significant decrease of activity in the alpha (p=0.01) and sigma (p=0.000) bands. No significant decrease in the theta (p=0.05), beta (p=0.129), or gamma (p=0.062) bands was found. Conclusion: PLMS without MA was found to be associated with EEG change characterized by increase in the delta frequency band. This finding seems to be compatible with the hypothesis of an integrative hierarchy of arousal responses of Sforza's. Considering that the subjects had lower PLMS index and shorter PLMS duration than those of the previous study, it is suggested that an even less severe form of PLMS without MA could induce neurophysiologic change, which may potentially be of clinical significance.

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An fMRI study on the cerebellar lateralization during visuospatial and verbal tasks (공간 및 언어 과제 수행 시 소뇌의 편측화에 관한 뇌 기능 연구)

  • Chung, Soon-Cheol;Sohn, Jin-Hun;Choi, Mi-Hyun;Lee, Su-Jeong;Yang, Jae-Woong;Lee, Beob-Yi
    • Science of Emotion and Sensibility
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    • v.12 no.4
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    • pp.425-432
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    • 2009
  • The purposes of the study were to examine cerebellar areas and lateralization responsible for visuospatial and verbal tasks using functional Magnetic Resonance Imaging(fMRI). Eight healthy male college students($21.5\;{\pm}\;2.3$ years) and eight male college students($23.3\;{\pm}\;0.5$ years) participated in this fMRI study of visuospatial and verbal tasks, respectively. Functional brain images were taken from 3T MRI using the single-shot EPI method. All functional images were aligned with anatomical images using affine transformation routines built into SPM99. The experiment consisted of four blocks. Each block included a control task(1 minute) and a cognitive task(1 minute). A run was 8 minutes long. Using the subtraction procedure, activated areas in the cerebellum during the visuospatial and verbal tasks were color-coded by t-score. A cerebellar lateralization index was calculated for both cognition tasks using number of activated voxels. The activated cerebellar regions during the both cognition tasks of this study agree with previous results. Since the number of activated voxels of the left and right cerebellar hemisphere was almost same, there was no cerebellar lateralization for both cognition tasks.

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