• Proceedings of the Korea Society of Poultry Science Conference
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• 2005.11a
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• pp.72-73
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• 2005

This study was carried out to investigate the effects of extracts supplements of pine nut cone on the broiler performance and the cholesterol content, the lipid oxidation of serum and meat of broilers. The control group was fed a common basal diet without antibiotics and the treatment group were fed a common basal diet with extracts of pine nut cone of 500ppm(T1), illite 1% +extracts of pine nut cone of 500ppm(T2), pine nut cone powder of 2.5%(T3) for 5 weeks. The weight gain and the feed intake were significantly higher treatment than control. The cholesterol content of serum was significantly decreased in T1, T2 group. The breast was also significantly decreased in T1, T2 group. The thigh was not different among treatment, but the cholesterol content of serum was significantly lower in T1, T2 treatment than control. TBARS of the brest and the thigh showed significantly lower than control. POV of the brest and the thigh showed different among treatment, but there were no correlations among treatment.

• Biomedical Science Letters
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• v.6 no.4
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• pp.261-268
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• 2000

Fibrinolytic enzyme (FE-2) was purified from the fruiting bodies of Tricholoma saponaceum using DEAE-Cellulose chromatography and Mono-S column chromatography, The enzyme has a molecular weight of 18.23 kDa and include Zn$^{2+}$ ion as found by ICP/MS. The N-terminal amino acid sequence of the enzyme was A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V It has a pH optimum at pH 7.5, suggested that FE-2 was a neutral pretense. The activity of FE-2 was highly inhibited by EDTA and 1,10-phenanthroline, indicating that the enzyme is a metalloprotease. The activity of FE-2 was increased by $Mg^{2+}$, Zn$^{2+}$, Fe$^{2+}$, and Co$^{2+}$, but the enzyme activity was totally inhibited by Hg$^{2+}$. No inhibition was found with PMSF, E-64, pepstatin and 2-mercaptoethanol. The enzyme hydrolyzed both $A\alpha$ and B$\beta$ chains of human fibrinogen. The $\gamma$ chain was resistant to hydrolysis by FE-2.

• The Korean Journal of Mycology
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• v.28 no.1
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• pp.60-65
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• 2000

Two fibrinolytic enzymes were purified from the fruiting bodies of Tricholoma saponaceum. The enzymes have a molecular weight of 18(FE-1) and 18.2(FE-2) kDa, respectively, and include $Zn^{2+}$ ion as determined by ICP/MS. The N-terminal amino acid sequence of the two enzymes were exactly the same: A-L-Y-V-G-X-S-P-X-Q-Q-S-L-L-V. The activity of FE-1 was highly inhibited by EDTA and 1,10-phenanthroline, indicating that the enzyme is a metalloprotease. The activity of FE-1 was slightly increased by $Mg^{2+},\;Zn^{2+},\;Fe^{2+}\;and\; Co^{2+}$, however, the enzyme activity was totally inhibited by $Hg^{2+}$. Addition of $Zn^{2+}\;and\;Co^{2+}$ reversed the inhibition caused by 1,10-phenanthroline. It has a pH optimum at pH 7.5, suggested that FE-1 was a neutral protease. It shows the maximum fibrinolytic activity at $55^{\circ}C$, is completely inactivated above at $65^{\circ}C$.

• Korean Journal of Plant Taxonomy
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• v.41 no.2
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• pp.130-137
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• 2011

Pedicularis growing at Mt. Halla of Jeju Island is known as an endemic species of P. hallaisanensis Hurusawa. On the other hand, the plant is morphologically similar to P. amoena, P. spicata, and P. verticillata in gross morphology, so the taxonomic treatment of the taxon remains controversial. To clarify the taxonomic position of the plants, we examined external morphological characters and nuclear ribosomal DNA ITS sequences for P. hallaisanensis and its related species. The plants of Mt. Halla are clearly different from P. amoena and P. verticillata in the morphology of calyx lobes, the length of galea and lower lip, density of glandular hairs on plants, presences of the radical leaves after anthesis and molecular data. However, P. hallaisanensis is not clearly separated from P. spicata distributed in N. E. Asia on external morphological characters and DNA sequences of internal transcribed spacers. In this study, the morphological and molecular data suggested that P. hallaisanensis should be merged into the former species.

• Journal of Life Science
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• v.14 no.4
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• pp.560-566
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• 2004

Agrocybe aegerita is Hymenomycetes fungus belonging to the order Agaricales and family of Bolbitiaceae. Much less is known about liquid culture of Agrocybe aegerita. Thus, the present study was to investigate the liquid cultural characteristics of Agrocybe aegerita my-celium. The optimal medium for the mycelial growth and density was ME medium, optimal tem-perature and initial pH were 25$\pm$1$^{\circ}C$ and 5.5, respectively. And optimal culture time for mycelial growth was 12 days. The modified optimal medium compositions were dextrin 3% (w/v), yeast extract 2% (w/w), MgSO$_4$0.05% (w/v), and KH$_2$PO$_4$0.15% (w/v). Under optimal culture conditions, the mycelial growth of modified optimal medium was higher than that of ME medium.

• Applied Biological Chemistry
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• v.26 no.1
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• pp.41-46
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• 1983

The characteristics of crude polyphenol oxidase extracted from mushroom[Tricholoma matsutake(S. Ito et Imai) Sing.] were investigated. The enzyme showed highest affinity to pyrogaroll among trihydroxyphenols. Except for o-diphenols the enzyme was inactive in di-and monophenols. The optimum pH was about 4 and the optimum temperature ranged from 45 to $55^{\circ}C$. The enzyme activity was completely inhibited by $70^{\circ}C$ heat treatment for 2 min. $Cu^{++}$, $Fe^{++}$ and $Mg^{++}$ activated the enzyme at low concentration($10^{-1}mM$), but inhibited at high concentration (1mM). The most potent inhibitors were Na-diethyldithiocarbamate, L-ascorbic acid, L-cysteine and NaCl. The Km value with pyrogaroll was 0.88mM.

• Proceedings of the Korean Geotechical Society Conference
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• 2008.10a
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• pp.1630-1637
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• 2008

Jeju-do is a island formed by the volcanic activity and has more than 360 volcanic cones distributed widely along the long axis of the elliptically shaped island. The volcanic cones consist mainly of scoria, so called "Song-I" in the local dialect. In this study the chemical and soil mechanical properties of scoria being very different from those of the inland were investigated with the various tests. In the sieve-passing test the particle size of scoria had more than 10 of uniformity coefficient and gradation coefficient of 1 ~ 3, showing relatively homogenous distribution. Based on the uniformity classification, scoria was assorted into GW. In the large scale direct shear tested for measuring the mechanical strength of scoria the internal friction angle of red scoria was $37^{\circ}$ and that of black scoria was $36^{\circ}$. This indicated that there was no difference in the mechanical strength between two types of scoria. On the other hand, red and black scoria had $1.24{\times}10^{-3}$ to $3.55{\times}10^{-2}$ cm/sec of k values for the static water level permeability, thus being classified into a coarse or fine sand as compared with that representing the saturated soil. They also had 1.411 to $1.477\;g/cm^3$ of notably low $r_{dmax}$ values for the compaction test as compared with common soil, which was considered to be due to their low specific gravity and high porosity. In conclusion, the soil mechanic properties of scoria obtained from this study are thought to be very helpful for reducing lots of trial and error happening in the civil engineering construction.

• Korean Journal of Food Science and Technology
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• v.16 no.2
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• pp.182-184
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• 1984

In order to develop the storage method of pine agaric by irradiation, pine agarics irradiated with 1,2 and 2.5 kGy were stored in natural low temperature storage room ($15{\pm}2^{\circ}C$, RH: $80{\pm}5%$) and the physicochemical properties were investigated during the 15 days of storage. Veil opening rate of pine agaric was 97% after 7 days storage in control, whereas only 5% in 2-2.5 kGy irradiated groups. Rotting rate after 7 days storage were 28% in control,5-8% in 2-2.5 kGy irradiated groups. In comparison of weight loss, texture and appearance.2-2.5 kGy irradiated groups were better than control. Chemical composition of pine agaric was not remarkably changed by the irradiation and storage period except a slight increase in reducing sugar and a decrease in ascorbic acid by the increase of irradiation dose.

• KSBB Journal
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• v.15 no.1
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• pp.9-13
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• 2000

In order to determine the characteristics of $\beta$-glucosidase associated with cellulose degradation, the enzyme produced extracellularly by the mycelia of Tricholoma matsutake DGUM 26001 in culture broth was partially purified. The enzyme activity was maintained in the range of temperatures trom 55 to $70^{\circ}C$ and its optimum temperature was $65^{\circ}C$. The $\beta$-glucosidase enzyme showed relatively high activity in the range of pH 3.0-5.0 and its optimum pH was 4.0. Under the optimal conditions, the specific activity of $\beta$-glucosidase for salicin as a substrate was 18.7 unit/mg protein. After thermal treatment of the enzyme at $55^{\circ}C$ for 60 min, more than 90% of the enzyme activity was still sustained. Iron($Fe^{++}$) stimulated enzyme activity, whereas mercury($Hg^{++}$) and copper($Cu^{++}$) inhibited. Compared to salicin as a substrate, the relative activity for cellobiose was observed to be 48.6%. The apparent $K_m$ and $V_{max}$ of the enzyme with cellobiose were 0.12 mM and 0.02 umol/min, respectively.

• Proceedings of the Korean Vacuum Society Conference
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• 2000.02a
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• pp.153-153
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• 2000

Si 산화는 반도체 공정상 필요한 과정으로 산업적으로나 학문적으로 중요하고 많이 연구되었다. 이중에서 Si(1110-7x7표면에서 초기 흡착된 산소는 준안정적 상태로 존재하며 표면온도, 산소의 노출량 그리고 진공도에 따라 그 수명이 제한된다. 이러한 준안정적 상태의 산소의 화학적 성질은 여러 표면분석장비가 동원되어 연구되었으나 아직까지 논쟁이 되고 있다. 이 경우 산소가 어떤 상태로 존재하는가는 표면화학종을 검출함으로서 해결될 수 있다. 저에너지 Cs+ 이온 반응성 산란은 이러한 요구를 충족시킬수 있는 가장 적합한 실험 방법중의 하나이다. 저에너지 Cs+ 이온 산란의 특징 중의 하나는 입사된 Cs+ 이온이 표면에 흡착된 화학종과 충돌후 탈착되면서 반응을 하여 송이 이온을 형성한다는 것이다. 이 송이 이온을 관측함으로서 표면에 존재하는 화학종을 알아 낼 수 있다. 이에 산소가 흡착된 Si(111)-7x7 표면에서의 산소의 준안정적 상태가 저에너지 Cs+ 이온 산란 실험을 통하여 연구되었다. 실험은 0.2-2L(1Langmuir = 10-6 Torr x 1 sec) 산소 노출량과 -15$0^{\circ}C$ - $25^{\circ}C$의 표면온도 그리고 5eV - 20eV의 Cs+ 이온 충돌에너지에서 CsSiO+ 이온이 유일한 생산물로서 검출되었다. CsSiO+ 이온은 입사된 Cs+ 이온과 표면에 존재하는 SiO 분자가 충돌 후 반응하여 탈착된 것으로 생각된다. 이것은 낮은 산소 노출량 즉, 초기 산화 단계에서 SiO가 표면에 존재한다는 것을 의미한다. 즉, 산소 분자는 산화단계의 초기에 해리되어 표면에 흡착되고 선구물질인 SiO를 형성함을 제시한다. 최근의 이론적 계산인 density functional calculation에서도 산소분자가 Si(111)-7$\times$7 표면의 준안정적 산화상태의 선구물질일 가능성을 배제한다. 이는 본 저에너지 Cs+ 이온 반응성 산란실험을 뒷받침하는 계산 결과이다. 높은 Cs+ 이온 충돌에너지에서 CsSi+, Si+, SiO+, Si2+, Si2O+ 등이 추가로 검출되었다. 이는 CsSi 이온을 제외하고 수 keV의 충돌에너지를 사용하는 이차 이온 질량 분석법과 비슷한 결과이다.