• 종축개량
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• v.9 no.11 s.46
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• pp.100-104
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• 2005

• 월간낙농육우
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• v.32 no.5
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• pp.130-132
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• 2012

• Journal of Veterinary Clinics
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• v.29 no.5
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• pp.395-399
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• 2012

In 2011, several herds in Youngju city in Gyeongbuk province underwent an outbreak of bovine viral diarrhea virus (BVDV) causing high morbidity and mortality. Genetic analysis revealed that two subgenotypes of BVDV-1b (n = 21) and BVDV-2a (n = 7) were identified. The BVDV-1b subgenotype was most frequently detected from our field cases and BVDV-2a subgenotype was also identified in this outbreak. These BVDV-1b infections showed severe acute clinical manifestations similar to BVDV-2 infection. This result reports the detection of BVDV-1b associated with an acute and fatal outbreak of BVDV in Korean indigenous calves.

• Journal of the korean veterinary medical association
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• v.24 no.6
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• pp.337-343
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• 1988

• Journal of the korean veterinary medical association
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• v.43 no.4
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• pp.315-319
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• 2007

최근 우리나라 소 사육농가에서 육성 및 자우에서 빈번하게 일어나는 세균성 및 바이러스성 설사병은 익히 알려져 있어 주의 깊게 관찰되어 치료와 예방을 잘하고 있으나, 정작 원충성인 콕시듐 설사병에 대해서는 간과하기 십상이다. 따라서 우리 대동물 임상수의사들도 특히 우상바닥 재료로 왕겨를 사용하는 농가들에 대해 관심깊게 봐두어 짚고 넘어가야 하지 않으면 아니되는 질병인 콕시듐증에 대해 정리하여 소개하고자 한다. 소 콕시듐병은 주로 포유기나 이유직후의 어린 소에서 발생하며, 포자층(Sporozoa)류에 속하는 Coccidia 원충(Potozoa)인 아이메리아(Eimeria) 및 Isospora속의 원충에 기인한 접촉성장염으로 장점막의 상피세포 파괴로 인한 급성출혈성 설사, 빈혈을 주 증상으로 하는 질병이며, 만성으로 될 경우는 성장지연과 생산성의 저하를 나타낸다.

• Journal of Veterinary Clinics
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• v.29 no.5
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• pp.377-383
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• 2012

Bovine diarrhea is a major economical burden to the bovine industry in Korea. Since multiple infectious agents can be involved in bovine diarrhea, differential diagnosis is essential for effective treatment. Therefore, a panel of two multiplex real-time PCR assays which can simultaneously detect six major bovine enteric pathogens [i.e., bovine viral diarrhea virus (BVDV), bovine coronavirus (BCoV), group A bovine rotavirus (BRV), Salmonella spp., Escherichia coli (E. coli) $K99^+$, and Cryptosporidium parvum] was developed and applied to test 97 fecal samples collected from cattle farms in Korea. In addition, microscopic examination was also preformed on the samples to detect Coccidium oocyst. The estimated sensitivity of the multiplex PCR was 0.1 $TCID_{50}$ for BVDV, BCoV and group A BRV, 5 and 0.5 CFU for E. coli $K99^+$ and Salmonella, respectively, and 50 oocysts for Cryptosporidium. The amplification efficiency of the multiplex PCR ranged between 0.97 and 0.99 for each pathogen. Among 97 samples, 36 samples were positive for at least one of the 6 major pathogens and 6 samples were simultaneously positive for 2 pathogens by the multiplex PCR assay. Coccidium oocysts were also detected in 48 samples, which were all collected from over 1 month old calves. In conclusion, the multiplex real-time PCR panel can be a useful tool for fast and accurate diagnosis of calf diarrhea associated with BVDV, BCoV, group A BRV, E. coli $K99^+$, Salmonella, and/or Cryptosporidium and Coccidium may be an important target which needs to be included in the multiplex PCR panel in the future.

• Korean Journal of Veterinary Research
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• v.37 no.1
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• pp.155-159
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• 1997

Torovirus-like particles이 심급성의 설사로 폐사한 송아지의 분변으로 부터 검출되었다. 설사로 폐사한 송아지는 2주령으로 설사증상을 보인 5두중 2두였으며 심한 설사증상을 보인지 24시간만에 급성폐사를 나타내었다. 나머지 3두도 심한 설사증상을 보였으나 항생제 및 수액요법으로 치료를 한 후 회복되었다. 전자현미경으로 관찰된 바아러스 입자는 Woode et al이 관찰한 Torovirus 입자와 동일하였으며 그 크기는 원형입자일 경우 70~90nm의 크기로 8~10nm의 Peplomer를 가지고 있었다. 그러나 바이러스이 입자형태는 소, 돼지 그리고 사람에서 보고된 Torovirus와 마찬가지로 다양하였으며 Tube 형태로 된 것은 길이가 약 150nm에 달하기도 했다. 이 보고는 국내에서의 송아지 Torovirus 감염증의 첫 보고이며 국내에서도 Torovirus가 존재한다는 것을 입증한 것이다.

• Korean Journal of Veterinary Research
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• v.32 no.3
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• pp.389-398
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• 1992

Molecular cloning was carried out on the Danish strain of bovine viral diarrhea virus(BVDV) to construct strategy for the diagnostic tools and effective vaccine of BVD afterwards. A recombinant DNA clone(No. 29) was established successfully from cDNA for viral RNA tailed with adenine homopolymer at 3'-end. $^{32}P$-labeled DNA probes of 300~1,800bp fragments, originating from the clone 29, directed specific DNA-RNA hybridization results with BVDV RNA. Recombinant DNA of the clone 29 was about 5,200bp representing 41.6% of the full length of Danish strain's RNA, and restriction sites were recognized for EcoR I, Sst I, Hin d III and Pst I restriction enzymes in the DNA fragment.

• Korean Journal of Veterinary Research
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• v.35 no.2
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• pp.287-295
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• 1995

Molecular cloning and nucleotide sequencing were undertaken for the RNA genome of gp53 antigenic region in cytopathic Singer strain of bovine viral diarrhea virus. The cloned cDNA was 939 nucleotides in length having a base composition of 31.0% A, 19.6% C, 25.5% G and 24.0% T. The sequence was corresponded to approximately 77.8%(817 bases) of predicted gp53 region and 122 bases after 3'end of gp53 region in the Singer strain when compared with NADL strain of known sequence. A single open reading frame was found in the sequence of 2nd frame and was deduced as encoding 312 amino acids.

• Journal of Veterinary Clinics
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• v.24 no.2
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• pp.150-153
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• 2007

The aim of this study was to investigate the prevalence of bovine viral diarrhea virus (BVDV) infection in Chonbuk province. Blood samples were taken from 92 korean calves to determined their serological status against BVDV, Capture enzyme-linked immunosorbent assay (ELISA) was used to test for antigen. The number of seropositive calves ranged from 3.3% to 12.9%. Antigens against BVDV were detected in 3.3% of healthy calves, 6.4% of digestive symptom calves, 12.9% of respiratory symptom calves, respectively. Sex and age of calves had no significant differences on the prevalence of BVDV. The results indicate that transmission of BVDV may have become exposed as a result of contact with acute infected or persistently infected cattle.