• Journal of Animal Science and Technology
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• v.45 no.2
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• pp.211-218
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• 2003

For the Exp. 1, a total of sixty pigs (10.57$\pm$0.30kg average initial body weight) were used in a 15-d growth assay to determine the effect of dietary $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase on growth performance and nutrient digestibility. Dietary treatments included 1) CON (corn-dried whey-SBM based diet), 2) EC0.1 (CON diet+0.1% enzyme complex of $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase). Through the entire experimental period, gain/feed of pigs fed EC0.1 diet was higher (0.43 vs 0.52) than that of pigs fed CON diet (P<0.05). Pigs fed EC0.1 diet showed significant (P<0.05) improvement in dry matter (74.82% vs 82.41%) and nitrogen (70.59% vs 77.88%) digestibilities compared to pigs fed CON diet. For the Exp. 2, a total of thirty six pigs (22.30$\pm$0.45kg average initial body weight) were used in a 30-d growth assay to determine the effects of dietary $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase in low energy diet on growth performance and nutrient digestibility. Dietary treatments included 1) AME (adequate ME diet), 2) AME+EC0.1 (AME diet+0.1% enzyme complex) and LME+EC0.1 (low ME diet + 0.1% enzyme complex). Through the entire experimental period, average daily feed intake of pigs fed enzyme complex supplemented diets was higher than that of pigs fed CON diet (P<0.05). Also, pigs fed AME+EC0.1 diet showed significant (P<0.05) increase in ADFI (1,401g vs 1,733g) compared to pigs fed CON diet. Pigs fed enzyme complex supplemented diet showed significant (P<0.05) improvement in dry matter and nitrogen digestibilities compared to pigs fed CON diet. In conclusion, the results obtained from these feeding trials suggest that the supplementation of $\alpha$-1,6-galactosidase and $\beta$-1,4-mannanase was an effective means for improving growth performance and dry matter and nitrogen digestibilities in nursery and growing pigs.

• Korean Journal of Poultry Science
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• v.46 no.2
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• pp.117-125
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• 2019

The aim of the study was to investigate the effects of dietary supplementation of zinc (Zn) sources (zinc oxide and Zn-methionine) on performance, organ weights, blood biochemical profiles, and digestive enzymes of the pancreas and small intestine in Korean native chicks (KNC). A total of 144 KNC (n=6) were fed a basal diet (CON, 100 ppm of Zn), a basal diet supplemented with 50 ppm of Zn with ZnO (ZNO), or a basal diet supplemented with 50 ppm of Zn with Zn-methionine (ZMT) for 28 days. There was no significant difference in body weight, gain, feed intake, and feed conversion ratio among the three groups. The relative weights of the liver, spleen, and intestinal mucosa were unaffected by the dietary source of Zn, whereas pancreas weight in the ZNO group decreased (P<0.05) compared with that in the CON and ZMT groups. Blood biochemical components including aspartate aminotransferase, and alanine aminotransferase were unaffected by dietary Zn supplementation. Pancreatic trypsin activity in the ZNO and ZMT groups was significantly (P<0.05) enhanced compared with that in the CON group. However, the activities of ${\alpha}$-amylase and carboxypeptidase A were not altered by dietary Zn supplementation. The activities of maltase and sucrase were unchanged, whereas the activity of leucine aminopeptidase tended (P=0.08) to be increased by dietary Zn supplementation. In conclusion, the supplementation with 50 ppm of ZnO or Zn-methionine resulted in an activation of protein digestive enzymes in the pancreas and small intestine without affecting animal performance in KNC.

• Korean Journal of Food Science and Technology
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• v.30 no.6
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• pp.1345-1349
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• 1998

The flavor constituents and functionality of small kingfish were studied as affected by two stage enzyme hydrolysis (TSEH). Total free amino acid contents in water extract, autolytic extract and TSEH of small kingfish were 541.3 mg%, 8,245.3 mg% and 6,636.6 mg%, respectively. Major free amino acids in TSEH were hydroxyproline, glutamic acid, proline, leucine, phenylalanine, lysine, arginine. As for nucleotides and other bases, IMP, TMAO and total creatinine were principal components in TSEH. And the major inorganic ions in TSEH were Na, K, P, Cl and $PO_{4}$. Also TSEH of small kingfish revealed very higher Angiotensin-I converting enzyme inhibition effect than those of water and autolytic extract.

• Korean Journal of Food Science and Technology
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• v.53 no.4
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• pp.365-374
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• 2021

In this study, the effect of enzyme-modified starch used in the preparation of filled hydrogel powder loaded with curcumin (FHP) on redispersibility, thermal and UV stability, and curcumin retention during in vitro digestion was investigated. FHP maintained stability without layer separation when redispersed and showed more stability against UVB than the emulsion powder (EMP). There was no significant difference in the chemical stability of curcumin between rice starch-based filled hydrogel powder (RS-FHP) and enzyme-modified starch-based filled hydrogel powder (GS-FHP). However, the gel matrix of GS-FHP maintained greater stability of lipid droplets in the stomach compared to RS-FHP, thereby improving the retention rate of curcumin after in vitro digestion. GS-FHP could be used as a novel material for developing premixes that require stable formulation and maintenance of functional substances, as it can increase the dispersion stability and retention rate of functional substances after digestion.

• Korean Journal of Plant Resources
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• v.35 no.5
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• pp.574-585
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• 2022

This study was conducted to quantify chlorogenic acid content and evaluate biological activity, such as antioxidant, antibacterial, anti-inflammatory, and digestive enzyme activity of Aralia elata ethanol extract (AEE). The SC₅₀ of DPPH and ABTS radical scavenging activities of AEE were 4.79±0.05 mg/mL, 5.79±0.05 mg/mL; total polyphenol and total flavonoid contents were 170.0±1.8 mgGAE/g, 105.5±4.1 mgQE/g, respectively. Nitric oxide (NO) was increased in RAW 264.7 cells and Caco-2 cells with treatment of LPS, and production of NO was inhibited by AEE in a concentration-dependent manner. Production of NO was reduced by 60.0±1.1% in RAW 264.7 cells and 50.7±2.8% in Caco-2 cells at of AEE. Similarly, the production of inflammatory cytokines (TNF-α, IL-1β and IL-6) was inhibited in a concentration dependent manner. Antibacterial activity increased as the dose concentration of AEE increased, and the MIC was 75 mg/mL for L. monocytogenes, and 100 mg/mL for S. typhimurium and H. pylori. In addition, amylase and protease enzyme activity was observed in AEE and increased enzyme activity was observed according to the concentration of the extract. AEE contained 7.06±0.01 mg/g of chlorogenic acid. As a result of the experiment, it is judged that it can be used as basic data for the development of health food using Aralia elata.

• Korean Journal of Microbiology
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• v.47 no.3
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• pp.255-262
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• 2011

Six protein-degrading bacteria were isolated from digestive organ of Harmonia axyridis. These isolates were categorized as Staphylococcus sciuri subsp. sciuri (3 strains), Bacillus subtilis (1 strain), and Bacillus thuringiensis (2 strains) by 16S rRNA gene sequence analysis. The Staphylococcus sp. CB2-3 was selected as a protease-producing bacterium which showed the highest protease activity of 58.5 U/ml at the pH 5.0 medium. The optimal pH and temperature of protease activity were pH 5.0 and $40^{\circ}C$, respectively. This acid protease had a relatively high stability of 80% between $30-50^{\circ}C$ at broad temperature range. The opimal medium compositions of carbon, nitrogen and mineral source for cell growth and protease activity were investigated. When sorbitol (0.5%) was used as carbon source, enzyme activity was increased about 2 times than that of the basal medium. When skim milk (0.5%) was used as nitrogen source, activity was increased about 2.5 times than that of the control. Cell growth and enzyme activity were increased by mineral source such as KCl, $K_2HPO_4$, $FeSO_4$, but was completely inhibited by divalent ions such as $Co^{2+}$, $Zn^{2+}$, $Mn^{2+}$, $Cu^{2+}$.

• Journal of the Korean Society of Food Science and Nutrition
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• v.29 no.4
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• pp.663-668
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• 2000

To develop natural intermediate flavoring substances, optimal processing conditions and qualities for two stage enzyme hydrolysate (TSEH) from low-utilized small longfinned squid were investigated. The optimal conditions for TSEH method were found as digestion with Alcalase (0.2% w/w-sample, pH 8.0) at 55$^{\circ}C$ 3 hours at the 1st stage and with Neutrase (exo-peptidase, 0.2% w/w-sample, pH 6.0) at 45$^{\circ}C$ for 2~3 hours at the 2nd stage. Among the method of water extract, autolytic extract and various kinds yields, transparency and organoleptic taste. From the results of chemical experiments and sensory evaluation, longfinned squid TSEH is flavorable as the natural intermediate taste-active substances for fisheries products such as soup base, squid-taste pasty and snacks.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.10a
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• pp.268-269
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• 2000

액젓은 신선한 선어를 염장하여 자가소화 효소 및 미생물의 작용에 의하여 원료가 분해ㆍ숙성된 액상의 전통 발효식품으로 예로부터 간장 대용, 김치의 부재료로서 널리 사용되어져 왔다. 액젓은 숙성기간 중에 미생물기원의 단백질분해 효소의 작용에 의하여 어육 단백질이 여러 형태의 Peptide로 분해되어 액젓의 풍미를 향상시키며, 항암, 혈압강화, 혈청콜레스테롤강하, 면역증강, 칼슘흡수 촉진 등 광범위한 생리활성기능을 나타내는 것으로 알려져 있다(M. Messina., 1995). (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2000.05a
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• pp.116-117
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• 2000

명란 젓갈은 명란에 식염 등을 가하여 염장함으로써 부패균의 번식을 억제하고 자가소화효소 및 미생물의 효소작용에 의해 분해, 숙성시킨 우리 나라 전통의 발효식품으로 독특한 감칠맛을 가지고 있어 옛부터 밥반찬으로 많이 이용되어 왔다. 그러나 저장 유통 중 품질의 변화가 심하여 현재 유통되는 조미 명란 제품은 냉장 및 냉동 보관해야 하므로 고품질 젓갈제품의 생산을 위해서는 새로운 공정 개발이 필요한 품목이다. (중략)

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.91-92
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• 2001

젓갈은 전통적인 저장발효식품으로 어패류의 육질, 내장 및 생식소 등을 원료로 하여 염장하였을 때 육자체에 함유된 자가소화효소와 젓갈 중의 미생물이 분비하는 효소작용에 의하여 원료 물질이 분해되면서 독특한 풍미를 갖게 된다(Park et al., 1996). 창란젓갈은 명태의 위와 창자를 원료로 하여 만든 젓갈로, 식염 함량이 8％이하의 저염양념젓갈의 형태로 제조되고 있으며 거의 전 과정이 정치 상태에서 진행되기 때문에 염장 및 숙성 시간이 오래 걸릴 뿐만 아니라 용기 상하간에 품질 편차가 커서 제품이 균일하지 못하다. (중략)