• Proceedings of the Korean Environmental Sciences Society Conference
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• 2001.05a
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• pp.77-81
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• 2001

• Journal of Food Hygiene and Safety
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• v.17 no.1
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• pp.8-14
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• 2002

Cotinine, one of nicotine metabolites, has been blown to reduce 4-(methylnitro samino)-1-(3-pyridyl)-1-butanone(NNK)- induced $O^{6}$MeG DNA adducts significantly in A/J mice when administered together with NNK. In order to examine the effects of phyto-extract mixture on the conversion of cotinine from nicotine, cellular and clinical experiments were carried out. When the phyto-extract mixture was added to culture media, human liver cells (FLCFR5) produced cotinine from nicotine 2~3 times compared to the control. The phyto-extract mixture which was microinjected into Xenopus oocyte along with nicotine showed the almost similar production of cotinine compared with the results of hepatic cell culture. In clinical test employing 17 to 20 healthy men, concentrations of urinary cotinine derived from smoking after taking photo-extract mixture increased up to 2 times compared to the control group. These results indicatethat the phyto-extract mixture can increase the metabolic efficiency of nicotine to cotinine, leading to the reduced formation of $O^{6}$MeG DNA adducts.

• Journal of Korean Biological Nursing Science
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• v.14 no.2
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• pp.103-111
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• 2012

Purpose: The purpose of this study was to identify the effect of self-efficacy promotion of the smoking cessation program on the amount of smoking, carbon monoxide level, urine cotinine level and the smoking cessation self-efficacy of adolescent smokers. Methods: The subjects composed of 24 for the experimental group and 28 for the control group who were smoking adolescents at the Middle School in G city. The data collection was done from October 13 to November 18, 2010. A nonequivalent control group pretest-posttest design was used. This program was composed on the basis of Shin (1997)'s Self-Efficacy Promoting Program. The self-efficacy promotion smoking cessation program was conducted for 2 hours per week, and lasted for 6 weeks on the experimental group. The control group had a general smoking program. Data was analyzed using SPSS/WIN 14.0 program. T-test was used to analyze outcome measures. Results: The amount of smoking, CO level, urine cotinine level, and self efficacy level of the experimental group was significantly improved more than that of the control group after intervention. Conclusion: Therefore, self-efficacy promotion smoking cessation program for adolescent smokers can be recommended for smoking cessation of adolescents.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.4
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• pp.188-198
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• 2016

This study examined the effects of a smoking cessation coaching program based on the motivation stage applying the Transtheoretical Model to stop the smoking of patients in terms of the amount of smoking, nicotine dependence, CO level, and urine cotinine. The study design was a multi-repeat multiple repeat intermittent time series study with one-group, a pre-post design. The participants were 47 smoking patients (44 males and 3 females), who were treated at a public hospital in N city. The participants were 4 (8.51%) subjects in the action stage and 43 subjects (91.49%) in the preparation stage of the motivation stage. The coaching program intervention was conducted at the first day, second week, and 6th week. The smoking cessation maintenance of the subjects was checked at the 12th week. A chi-square test and t-test were used to analyze the data. The subjects in the action stage were kept under the condition of no smoking and nicotine dependence. After the program of subjects in the preparation stage, the amount of smoking, nicotine dependence, and CO level were significantly lower compared to the pre-test (p<.001). The findings suggest that the coaching program based on the motivation stage was effective in improving the smoking cessation for patients who smoke. On the other hand, the patients in the smoking cessation program may require much more financial assistance than those of healthy people. A greater workforce and budget will be needed for patients to stop smoking.

• Journal of Korean Academy of Nursing
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• v.30 no.1
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• pp.110-121
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• 2000

This study was designed to develop the life moking cessation program with multidisciplinary approach using the Bandura's theory of self-efficacy and confirm the effect of it on the self-efficacy and smoking amount in college students. For this purpose non-equivalent control group, pretest-posttest design was used. The participating subjects in this study were 46 male college freshmen, 23 in experimental and 23 in control group. The experimental group received the 8 sessions of lecture and small group discussions for 4 weeks and a telephone coaching program for a period of 8 weeks. The control group received 5 sessions of lecture without further treatments. The data was collected from March 15 to June 11, 1999, and analysed by SAS/PC program with $\chi$$^2$test, simple t, paired t test. The results were as follows. 1. The score of self-efficacy was significantly increased over time in the experimental group than those of the control group. 2. The amount of urine cotinine was decreased over time in the experimental group, number of cigarettes smoked a day were significantly decreased, and they were significantly less than those of the control group. In conclusion it was found that the Life Smoking Cessation Program with Multidisciplinary Approach was an effective nursing intervention for increasing self efficacy and reducing the amount of smoking in male college students. Therefore, future smoking cessation programs should always consider the concept of self-efficacy and ways to positively reinforce it.

• Research in Community and Public Health Nursing
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• v.12 no.3
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• pp.716-731
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• 2001

The purpose of this study was to test the effect of a self-efficacy promotion smoking cessation program for middle school students. This program was redesigned on the basis of Shin Sung Rye( 1997)' s Self-Efficacy Promoting Program for this study. The design of this paper was quasi-experimental. equivalent control group pre-post test. time series design. The subjects of this study were 53 smoker adolescents in D Middle School in the city of Busan. The results were summarized as follows: 1) The Hypothesis 1 was accepted: The self efficacy of the experimental group was higher than that of the control group after 1 week (expected efficacy t=2.20, p<.05. expected outcomes t=-2.58. p<.05) 4 weeks after education (expected efficacy t=- .19. p<.001, expected outcomes t=-2.586. p<.05). 2) The Hypothesis 2 was accepted: The amount of smoking of the experimental group was reduced more than that of the control group after 1 week (t=2.05, p<.05) and after 4 weeks (t=2.03. p<.05). 3) The Hypothesis 3 was accepted: The positive urine cotinine of the experimental group was less than that of the control group after 1 week after education($x^2$=8.57. p<.01) after 4 weeks ($x^2$=22.49. p<.001). In conclusion. a self-efficacy promotion smoking cessation program for middle school students was an effective smoking cessation program and then it will be valuable for stopping the smoking among the adolescents.

• Environmental Analysis Health and Toxicology
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• v.16 no.4
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• pp.181-188
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• 2001

A gas chromatographic method was established for the simultaneous determination of urinary nicotine and cotinine. The analytes in basified urine containing a sufficient amount of Na$_2$S0$_4$were extracted into dichloromethane by vigorous shaking. Into the transferred organic phase was added a small amount of acidified methanol (0.5 N HCI in methanol), followed by concentrating the mixture to dryness using a mild stream of nitrogen gas. The concentrate was reconstituted with methanol and the final solution analyzed using the gas chromatograph equipped with the nitrogen-phosphorus detector. The reproducibility tests showed coefficients of variation less than 11％ for both compounds. The percent recovery for both analytes ranged from 88 to 103％. The estimated method detection limits for nicotine and cotinine were 0.60 and 5.1 ng/mL, respectively. Extraction efficiencies for both nicotine and cotinine apparently declined without the addition of Na$_2$S0$_4$into the urine. Moreover, the absence of methanolic HCI in the extract resulted in almost complete evaporation of nicotine and partial loss of cotinine during the concentration process, indicating that the formation of nicotine-HCI and cotinine-HCI species is prerequisite to the suppression of the loss of both compounds.

• Journal of Preventive Medicine and Public Health
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• v.40 no.4
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• pp.297-304
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• 2007

Objectives : We investigated the validity of the dipstick method (Mossman Associates Inc. USA) and the expired CO method to distinguish between smokers and non-smokers. We also elucidated the related factors of the two methods. Methods : This study included 244 smokers and 50 ex-smokers, recruited from smoking cessation clinics at 4 local public health centers, who had quit for over 4 weeks. We calculated the sensitivity, specificity and Kappa coefficient of each method for validity. We obtained ROC curve, predictive value and agreement to determine the cutoff of expired air CO method. Finally, we elucidated the related factors and compared their effect powers using the standardized regression coefficient. Results : The dipstick method showed a sensitivity of 92.6%, specificity of 96.0% and Kappa coefficient of 0.79. The best cutoff value to distinguish smokers was 5-6ppm. At 5 ppm, the expired CO method showed a sensitivity of 94.3%, specificity of 82.0% and Kappa coefficient of 0.73. And at 6 ppm, sensitivity, specificity and Kappa coefficient were 88.5%, 86.0% and 0.64, respectively. Therefore, the dipstick method had higher sensitivity and specificity than the expired CO method. The dipstick and expired CO methods were significantly increased with increasing smoking amount. With longer time since the last smoking, expired CO showed a rapid decrease after 4 hours, whereas the dipstick method showed relatively stable levels for more than 4 hours. Conclusions : The dipstick and expired CO methods were both good indicators for assessing smoking status. However, the former showed higher sensitivity and specificity and stable levels over longer hours after smoking, compared to the expired CO method.

• Journal of Environmental Health Sciences
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• v.47 no.4
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• pp.320-329
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• 2021

Background: Smoking exposure may be objectively assessed through specific biomarkers. The most common biomarker for smoking is cotinine concentration in urine, and setting an optimal cut-off point can accurately classify smoking status. Such a cut-off point for Korean adolescents has never been studied. Objectives: The aim of this study was to determine a cut-off point for urinary cotinine concentration for the discrimination of smoking in adolescents. Methods: Participants were adolescents aged 13~18 years who participated in the third cycle of the Korean National Environmental Health Survey. We used urine samples to confirm the level of cotinine concentrations. Smoking status was determined by self-reported questionnaire. We identified the optimal cotinine cut-off point for discriminating smoking status using receiver operating characteristic curve analysis. Results: Of the 904 participants, 28 (3.1%) were smokers, among whom 20 (71.4%) were male. The median urinary cotinine concentrations in smokers was 218 ㎍/L (male: 215 ㎍/L, female: 303 ㎍/L), and that in non-smokers was 1.31 ㎍/L (male: 1.46 ㎍/L, female: 1.18 ㎍/L). We found significant differences in urinary cotinine concentration according to smoking status and sex (p<0.001). Urinary cotinine concentrations performed well for identifying smoking adolescents [area under the curve: 0.954 (male: 0.963, female: 0.908)]. The cut-off that optimally distinguished smokers from non-smokers was 39.85 ㎍/L (sensitivity: 89.3%, specificity: 97.4%). Male [39.85 ㎍/L (sensitivity: 90.0%, specificity: 94.9%)] had a different optimal cut-off point than female [26.26 ㎍/L (sensitivity: 87.5%, specificity: 99.6%)]. Conclusions: This study determined a cut-off point for urinary cotinine of 39.85 ㎍/L (male: 39.85 ㎍/L, female: 26.26 ㎍/L) to distinguish smokers from non-smokers in adolescents.

• Journal of Environmental Health Sciences
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• v.48 no.4
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• pp.216-226
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• 2022

Background: The amount of smoking in adolescence increases with a younger age of smoking initiation and affects physical health. To establish and evaluate smoking-related policies, it is important to determine actual smoking status. Validation of self-reported questionnaires can identify the accuracy of the questionnaire data reflecting smoking status. Objectives: The purpose of this study was to evaluate the validity of self-reported smoking status and identify factors affecting the accuracy of self-reported smoking in South Korean adolescents. Methods: This study investigated the consistency between cotinine concentrations and self-reported questionnaire data through the analysis of urine samples collected from 922 adolescents aged 13~18 among the participants of Cycle 3 of the Korean National Environmental Health Survey. Smoking status was classified using the cotinine cut-off point of 39.85 ㎍/L in adolescents, and factors affecting the accuracy were analyzed through multiple logistic regression analysis. Results: The smoking rates according to the self-reported questionnaire and cut-off point-based cotinine concentrations among adolescents were 3.1% and 5.1%, respectively. The results found 97.1% consistency between self-reported smokers and smokers according to cotinine concentration. Factors affecting the discrepancy showed a significant relationship, including gender, secondhand smoke, and use of e-cigarettes. Conclusions: The results can be used as basic data to establish a smoking policy for adolescents through continuous monitoring and improvement of questionnaire items of factors affecting the discrepancy.