• Research in Plant Disease
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• v.18 no.2
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• pp.101-108
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• 2012

In 2006, pinewood nematode, Bursaphelenchus xylophilus, was isolated from about 50 years old trees of Pinus densiflora and P. koraiensis showing leaf-wilt and -drying symptoms in Gwangju, Gyeonggi-do and Chuncheon, Gangwon-do. Isolation of pinewood nematodes from sapwood of infected pine trees showed no difference in population density between tree species and among the sampling heights on the main stem. Migration of pinewood nematodes in the host tree were investigated by inoculation of red pine (P. densiflora, 3 years old) seedlings with B. xylophilus. The nematodes seemed to move in red pine seedlings prior to multiplication and it might have taken about 20 days to start multiplication and expression of symptoms including wilt and dieback. In initial time after inoculation, nematodes started migration through the cortical resin canal from inoculated site and further showed upward and downward movements. More nematodes were observed in cortical resin canal during early period of inoculation and later in resin canal of xylem and tracheid also while, the pith still remained free from nematode. The density of B. xylophilus was higher in seedlings of low-vigor with poor root growth than in seedlings of normal root growth. Seedlings showing high density of B. xylophilus exhibited stem discoloration and secondary infection by fungus at the inoculation site.

• Korean journal of applied entomology
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• v.42 no.4
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• pp.315-321
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• 2003

Larval entrance and adult emergence holes of Japanese pine sawyer (Monochamus alternatus), primary vector of pinewood nematode (Bursaphelenchus xylophilus), were measured in dead pine logs from 1999 to 2002. Their distributions within pine log were also analyzed. More numbers of entrance and emergence holes were distributed on crown than trunk part as 56.2 and 27.7 holes/m$^2$, respectively Higher proportions of entrance (27.5％) and emergence holes (22.4%) were distributed on the log with 8 to 10 cm diameter; the larger or the smaller logs had fewer holes. Surface area of entrance hole was 65.8 $\textrm{mm}^2$ and diameter of emergence holes was 7.0 mm in average. Average depth of pupal chamber was 24.8 mm from surface to the bottom of the chamber, and its volume ranged from 200 to 2.000 ㎣ Average distance between entrance and emergence holes on bark surface was 3.3 cm. Gallery length from the beginning of entrance hole to the end of emergence hole was 46.2 mm.

• Journal of Korean Society of Forest Science
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• v.95 no.3
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• pp.240-249
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• 2006

Recently, dispersion of damaged forest by pine wilt disease has been regarded as a serious social issue. Damages by pine wilt disease have been spreaded by natural area expansion of the vectors in the damaged area, while the national wide damage spread has induced by human-involved carrying infected trees out of damaged area. In this study, damaged trees were detected and located on the digital map by aerial photograph and terrestrial surveys. The spatial distribution pattern of damaged trees, and the relationship of spatial distribution of damaged trees and some geomorphological factors were geostatistically analysed. Finally, we maked natural spread pattern map of pine wilt disease using geostatistical CART(Classification and Regression Trees) model. This study verified that geostatistical analysis and CART model are useful tools for understanding spatial distribution and natural spread pattern of pine wilt diseases.

• Korean Journal of Remote Sensing
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• v.31 no.5
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• pp.385-394
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• 2015

Pine wilt disease has greatly damaged pine forests not only in East Asia including South Korea and China, but also in European region. The damage caused by pine wood nematode (Bursaphelenchus xylophilus) is expressed in bundles within stands and rapidly spreading, however, present field survey methods have limitations to detecting damaged trees at regional level. This study extracted the damaged trees by pine wilt disease using time series hyperspectral aerial photographs, and analyzed their distribution characteristics. Hyperspectral aerial photographs of 1 meter spatial resolution were obtained in June, September, and October. Damaged trees by pine wilt disease were extracted using Normalized Difference Vegetation Index (NDVI) and Vegetation Index green (VIgreen) of the September photograph. Among extracted damaged trees, dead trees with leaves and without leaves were classified, and the spectral reflectance values from the photographs obtained in June, September, and October were compared to extract new outbreaks in September and October. Based on the time series dispersion of extracted damaged trees, nearest neighbor analysis was conducted to analyze distribution characteristics of the damaged trees within the region where hyperspectral aerial photographs were acquired. As a result, 2,262 damaged trees were extracted in the study area, and 604 dead trees (dead trees in last year) with leaves in relation to the damaged time and 300 and 101 newly damaged trees in September and October were classified. The result of nearest neighbor analysis using the data shows that aggregated distribution was the dominant pattern both previous and current year in the study area. Also, 80% of the damaged trees in current year were found within 60 m of dead trees in previous year.

• The Korean Journal of Pesticide Science
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• v.13 no.3
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• pp.190-196
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• 2009

The residues of abamectin 1.8% EC, resisted for control of pine wood nematode, Bursaphelenchus xylophilus in pine tree were surveyed in tissue of Pinus thunbergii and P. koraiensis after injection of a liquid formulation. Limits of detection of abamectin in tissue of P. thunbergii were $0.05\;mg\;kg^{-1}$ and mean recoveries at $0.5\;mg\;kg^{-1}$ trunk injection were 90.9% and 93.1% respectively in stem and trunk of P. thunbergii. Abamectin 1.8% EC, trunk injected in 15 m height P. thunbergii were detected in all stem (edible part of carrier insect of pine wood nematode, Monochamus alternatus) from 0.29 to $0.73\;mg\;kg^{-1}$ after 150 days injection. Amount of residue of abamectin 1.8% EC in 12.6 cm mean breast height diameter (DBH) P. thunbergii were variable depending on individual trees in natural forest. Amount of residues in lower and middle part of trunk were reduced with the passage of the injection time. In upper part of trunk were detected $1.84\;mg\;kg^{-1}$ on 30 days after injection however $0.65\;mg\;kg^{-1}$ on 15 days after injection and under detection limit on 100 and 180 days after injection in P. thunbergii. Bottom and middle parts of crown were detected $0.183\;mg\;kg^{-1}$ and $0.173\;mg\;kg^{-1}$ respectively on 180 days after injection in P. thunbergii. Mean residues of abamectin in crown and trunk were $0.80\;mg\;kg^{-1}$ and $0.30\;mg\;kg^{-1}$ on 170 days after trunk injection in 20 cm DBH and 9 m height P. koraiensis. Mean residues of abamectin in crown and trunk were $0.67\;mg\;kg^{-1}$ and $0.36\;mg\;kg^{-1}$ on 170 days after trunk injection in 15 cm DBH and 6 m height P. koraiensis.

• The Korean Journal of Pesticide Science
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• v.16 no.4
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• pp.322-327
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• 2012

Fate of fenitrothion aerially sprayed to control pine wood nematode (Bursaphelenchus xylophilus) was studied in a forest of Haman area. And the monitoring of fenitrothion was conducted in a stream flowed from forest area of Gijang sprayed fenitrothion. Fenitrothion 50% EC was diluted 100 times and applied two or three times using helicopter in Haman and Gijang, respectively. Average fenitrothion deposits on forest floor ranged from 6% of standard aerial application rate. Following to the second application, fenitrothion deposits in the pine needle ranged from 0.6 to 0.9 mg/kg and then rapidly decreased to 0.01 mg/kg after 109 days. Deposits on the plant washed off by rainfall and reached to soil surface was 1.3% of the application rate. All of fenitrothion on the ground resided in the forest floor covering the soil surface, where fenitrothion residues were decreased to a tenth at 109 days after the second application, but they were not detected in sol beneath it. And the only low level of fenitrothion residues, 0.0009 mg/L, was detected in runnel of the experimental forest just after aerial application. The concentration of fenitrothion in effluent from Gijang area was less than detection limit (0.0001 mg/L) during the entire period.