• Journal of the Korean Institute of Intelligent Systems
• /
• v.14 no.4
• /
• pp.411-417
• /
• 2004

Recently, instruments and systems related on biological technology have been enormously developed. Particularly, many researches for biological cell injection have been carried out. Usually, excessive contact force occurring when the end-effector and a biological cell contact might make a damage on the cell. Unfortunately, the excessive force could easily destroy the membrane and tissue of the cell. In order to overcome the problem, we proposed a new injection system for biological cell manipulation. The proposed injection system can measure the contact force between a pipette and a cell by using a force sensor. Also, we used vision technology to correctly guide the tip of the pipette to the cell. Consequently, the proposed injection system could safely manipulate the biological cells without any damage. This paper presents the introduction of our new injection system and design concepts of the new micro end-effector. Through a series of experiments the proposed injection system shows the possibility of application for precision biological cell manipulation such as DNA operation.

• Proceedings of the Korean Society for Bioinformatics Conference
• /
• 2006.02a
• /
• pp.116-123
• /
• 2006

생물체의 cDNA를 유리기판위에 고밀도로 첨착 시킨 유전자 칩과 정량적인 방법으로 개별 유전자 발현을 진단 가능한 Real- time PCR (실시간 고분자중합연쇄반응 기술) 기법은 첨단 의학분야와 신약개발 및 독성유전체 연구분야에 활발히 도입되고 있는 기술이다. 본 발표의 첫 번째 부분에서는 유전자칩 에서 얻어진 유전자 발현패턴분석에 기반한 바이오마커 선정 및 real time PCR에 의한 확증 관련 기술 과 유전자칩에서 얻어지는 수많은 데이터를 재정렬 및 다양한 분석기법과 display기술을 활용하여 광범위한 화학물질에 대한 독성효과 분석을 가능하게 해주며, 특정 독성물질에 대한 관련유전자 그룹 발견 및 독성영향에 따른 분류방법에 관한 결과를 발표할 것이다. 또한 바이오마커 활용의 하나로 박테리아세포 기반 바이오센서 제작및 세포칩 개발등에 대한 결과도 추가될 것이다. 두 번째 부분에서는 non-model organism(유전체정보가 확보되지 않은 생물체)인 송사리를 이용하여 새로운 2K 유전자칩을 개발하고, 여기서 각종 화학물질에 대하여 얻어진 수많은 유전자칩 분석 데이타를 활용하여 각각의 화학물질이 보여주는 독성효과를 매우 효과적이고 쉽게 이해할 수 있는 display기술을 개발, 적용함으로써 유전자칩 발현에 기반한 화학물질 독성 screening 및 specificity discrimination을 가능케 하는 예가 발표될 것이다. 이 연구에서 개발한 송사리 유전자칩은 간조직의 RNA를 직접 cDNA화 하는 방식을 취하고 있어 전체 송사리의 유전정보를 필요로 하지 않아 비용 및 효율에서 전체 송사리의 유전정보를 얻는 비용과 노력을 취하지 않고 간에서 발생하는 독성학적 영향 및 유전자의 발현정도를 정밀하고 효율적인 방법으로 얻어 낸다. 현재 2000여개의 cDNA유전자중 50%이상의 유전자가 17베타에스트라디올, 페놀, 노닐페놀, 비스페놀, 감마레이조사, 잔류약품중 이보프란, 다이클로펜악, 농약중의 파라???R, 돌연변이 유발물질 중의 이티비알, 금속류중의 카드뮴을 통해 발현양상과 특정 캐미칼별 발현 특이성이 조사되었고, 이들 유전자는 염기서열 분석을 통해 염기서열이 분석되었으며, 미국 NCBI의 유전자 은행과의 비교를 통해 일부유전자는 새로운 유전자로 밝혀지고 있다. 또한 이 발표에서는 소염진통제계열 의약품인 dichlofenac 이 송사리의 각종 조직에 미치는 독성영향을 Real-time PCR을 이용하여 대표적 스트레스 유전자의 발현에 미치는 영향에 대한 분석 예가 발표될 것이다.

• Applied Chemistry for Engineering
• /
• v.23 no.6
• /
• pp.515-520
• /
• 2012

In this review, the fabrication of silicon based memory capacitor and organic memory thin film transistors (TFTs) was discussed for their potential identification tag applications and biosensor applications. Metal or non-metal nanoparticles (NPs) could be capped with chemicals or biomolecules such as protein and oligo-DNA, and also be self-assembly monolayered on corresponding target biomolecules conjugated dielectric layers. The monolayered NPs were formed to be charging elements of a nano floating gate layer as forming organic memody deivces. In particular, the strong and selective binding events of the NPs through biomolecular interactions exhibited effective electrostatic phenomena in memory capacitors and TFTs formats. In addition, memory devices fabricated as organic thin film transistors (OTFTs) have been intensively introduced to facilitate organic electronics era on flexible substrates. The memory OTFTs could be applicable eventually to the development of new conceptual devices.

• The Journal of the Korea Contents Association
• /
• v.21 no.4
• /
• pp.825-832
• /
• 2021

In this study, by selecting specific aptamers that selectively detection on P. gingivalis, the main cause of periodontal disease, and purifying and identifying protein molecules that bind to the selected aptamers, the mechanism of action of P. gingivalis was investigated. A DNA library having 39 random sequences was prepared, and aptamers with specificity for P. gingivalis were selected using the SELEX method, and the nucleotide sequence was analyzed by cloning using PCR2.1 cloning vector. 8 of aptamers with different nucleotide sequences were selected, and modified weston blot was performed using APG-3 among the selected aptamers to identify 11 proteins that act directly, and proteins were analyzed. As a result, a protein that selectively binds to P. gingivalis was isolated and identified. Therefore, aptamer selectively binds and attaches to proteins related to inhibition of sugar metabolism and cell activity of P. gingivalis, suggesting the possibility of a sensor for diagnosis of periodontal disease.

• Journal of Korea Game Society
• /
• v.13 no.5
• /
• pp.63-72
• /
• 2013

To enhance the effect of science educational contents, we developed a motion based training content: 3D space exploration. In this content, we used the 3D depth camera for user's motion recognition. Learners have to conduct the space station maintenance mission using the motion based natural and intuitive interface. The result this study is expected to propose the immersive training simulation for young science learners.

• Journal of Dairy Science and Biotechnology
• /
• v.29 no.2
• /
• pp.43-49
• /
• 2011

The increasing number of analytes in concern and the alarming health and environmental consequences have required effective means of monitoring for safety control. Biosensors offer advantages as alternatives to conventional analytical methods because of their inherent specificity, simplicity, and quick response. Colorimetric biosensor, one of biosensor group, is one of the easiest and the most convenient methods because detection can be done using naked eye. Recently, a novel method for rapid detection and read-out of specific immunoassays with naked eye using polydiacetylene (PDA) was developed. Polydiacetylene has recently been in the limelight as a transducing materials because of its special features that allow optical transduction of sensory signals and inherent simplicity and ease of use in supramolecular chemistry. Various forms of PDA are used as a sensor platform for detection of various biological analytes such as viruses, DNA, proteins, bacteria and hazardous molecules.

• KSBB Journal
• /
• v.24 no.1
• /
• pp.1-8
• /
• 2009

In the past decade, we have observed rapid advances in the development of biochips in many fields including medical and environmental monitoring. Biochip experiments involve immobilizing a ligand on a solid substrate surface, and monitoring its interaction with an analyte in a sample solution. Metal nanoparticles can display extinction bands on their surfaces. These charge density oscillations are simply known as the localized surface plasmon resonance (LSPR). The high sensitivity of LSPR has been utilized to design biochips for the label-free detection of biomolecular interactions with various ligands. LSPR-based optical biochips and biosensors are easy to fabricate, and the apparatus cost for the evaluation of optical characteristics is lower than that for the conventional surface plasmon resonance apparatus. Furthermore, the operation procedure has become more convenient as it does not require labeling procedure. In this paper, we review the recent advances in LSPR research and also describe the LSPR-based optical biosensor constructed with a core-shell dielectric nanoparticle biochip for its application to label-free biomolecular detections such as antigen-antibody interaction.

• Korean Chemical Engineering Research
• /
• v.49 no.4
• /
• pp.400-404
• /
• 2011

Many studies on polydiacetylene(PDA) have been investigated to apply to chemical and biological sensors due to their unique optical properties of color change from blue to red and fluorescence change from non-fluorescence to red fluorescence. Especially, high sensitivity against specific molecules is very important to apply polydiacetylenes to various sensors. In this study, we examined the effect of sensitivity enhancement of 10,12-pentacosadynoic acid(PCDA) vesicles in detection ${\alpha}$-cyclodextrin(CD) according to control of vesicle size by filters with different pore sizes and polymerization temperature. Colorimetric response(CR) was calculated using visible spectrometer. In order to investigate the effect of vesicle size on sensitivity of PDA vesicles, two PCDA vesicles were filtered without filtration and with 0.22 ${\mu}m$ filter. The two PCDA vesicles were polymerized at $25^{\circ}C$ and were incubated with ${\alpha}$-CD(5 mM) for 30 min. The CRs of the former and latter vesicles were 31.4% and 74.0%, respectively. Then, two PCDA vesicles filtered with 0.22 ${\mu}m$ filter were polymerized at $25^{\circ}C$ and $5^{\circ}C$ and were reacted with ${\alpha}$-CD(5 mM) for 30 min to examine the effect of polymerization temperature. The CRs of the former and latter vesicles were 74.0 and 99.2%, respectively. This suggests that vesicle sizes and polymerization temperature are key factors in enhancing the sensitivity of PDA vesicles. In addition, these results are expected to be useful to apply the PDA vesicles as biosensors to detect DNA, protein, and cells.