• Journal of Periodontal and Implant Science
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• v.35 no.4
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• pp.1003-1017
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• 2005

이 논문은 단일조사 저출력 $CO_2$ Laser조사가 치주인대 섬유아세포의 증식과 분화에 미치는 영향을 살펴보고 가장 효과적인 에너지와 파워밀도(power density)를 알아보기 위해 다음과 같이 실험하였다. 0.5W 출력, 10.6 ${\mu}m$ 파장, 50 Hz 연속형 $CO_2$ Laser를 사용하여, 실험군은 laser tip과 배양된 세포 사이의 거리를 2cm, 3cm으로 나누고, 조사시간을 1초, 3초로 나누어 4개의 군으로 설정하였고 대조군은 laser를 조사하지 않은 군으로 하였다. 치주인대 섬유아세포의 증삭정도와 골모세포로의 분화정도를 보기 위하여 각각 MTT 실험과 ALP activity 실험을 시행하여 다음과 같은 결과를 얻었다. 1. Laser를 조사하고 난 후 5일째에, 모든 군에서 유의하게 세포가 증식되는 것을 확인할 수 있었고 조사방법간에 유의한 차이가 없었다. 2. 대조군과 살험군에서 0일째에 비하여 3일째, 5일째, 7일째, 10일째에 통계적으로 유의 하게 ALP activity가 증가하였고, 이중 2cm,1sec 군을 제외하면 3일째에서 가장 높은 ALP activity 값을 보였다. 특징적으로 2cm,1sec 군은 3일째부터 10일까지 통계적으로 유의하지는 않지만 시간이 지남에 따라서 ALP activity가 증가함을 보였다. 7일과 10일째에는 2cm,1sec, 3cm,3sec군에서 다른 군에 비하여 큰 activity값을 보였다. 이번실험에서 저출력 $CO_2$ Laser 조사는 세포의 증식보다는 분화에 더 큰 영향을 끼쳤고, 2cm, 1sec, 3cm, 3sec 군이 치주인대 섬유아세포의 분화에 가장 효과적인 laser 조사방법으로 분석되었다.

• Journal of Life Science
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• v.13 no.6
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• pp.849-855
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• 2003

Nitric oxide (NO) plays an important role as a signaling molecule in the proliferation of placenta trophoblasts. In this study, we investigated the effect of NO on the activation of phospholipase C (PLC) in BeWo cells, choriocar-cinoma cell line. Sodium nitroprusside (SNP), an agent to produce NO spontaneously in cells, alone increased $［^3H］$ thymidine incorporation of BeWo cells, indicating NO stimulates proliferation of the cells. NO-induced proliferation of BeWo cells was blocked by U73122, an inhibitor of PLC, suggesting that NO-induced PLC activation is involved in the cell proliferation. NO also stimulated extracellular signal-regulated kinase (ERK) in BeWo cells, indicated by increased phosphorylation of ERK1/2 in Western blotting using anti-phospho-ERK1/2 antibody. NO-induced phos-phorylation of ERK1/2 was not abrogated by U73122. $PLC\gamma_1$l but not$PLC\gamma_2$ was tyrosine phosphorylated by SNP in immunoprecipitation assay using anti-$PLC\gamma_1$/$PLC\gamma_2$ antibodies, and SNP-induced phosphorylation of $PLC\gamma_1$ was abrogated by pre-treatment of cells with genistein and PD98059, indicating that NO induced-phosphorylation of $PLC\gamma_1$ is mediated by ERK. These results suggest that NO stimulates the proliferation of BeWo cells through ERK and $PLC\gamma_1$.

• Journal of Sericultural and Entomological Science
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• v.46 no.2
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• pp.72-76
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• 2004

We have studied the effect of silk proteins to the cell proliferation of human skin fibroblast cells (CCD-986sk) after injury. Silk proteins were extracted treatment with enzyme or NaOH solution from raw silk and culled-cocoon shell of Bombyx mori, Antheraea yamamai and A. pernyi. The cell proliferation after in vitro injury are increased in treatment by Bombyx mori (BM-1,2), Antheraea yamami (AY-1,2) and A. pernyi (AP-1,2). The silk protein fractions-treated cells exhibited proliferation in a dose dependent between $0.1\;{\mu}g/ml$ and $10\;{\mu}g/ml$. But, the macrophage, RAW 264. 7 cell viability was unaffected by the silk protein fractions by MTT assay. The molecular weights of the silk protein fractions were from 300-600 to 900-1500. These results results that the silk protein fractions may function through skin fibroblast proliferation.

• Journal of the East Asian Society of Dietary Life
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• v.19 no.4
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• pp.558-563
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• 2009

The inhibitory effects of land race radish (LRR) and wild race radish (WRR) extracts on cancer cell lines were investigated. A and their glucosinolate and sulforaphane contents were analyzed. The anticancer activitiesy of the LRR and WRR extracts on the breast cancer cell line MCF-7 were determined by a CCK (cell counting kit) assay, in which WWR showed higher inhibition rates than LRR. The sulforaphane content of WRR was higher than that of LRR. In the lung cancer cell line, A-549, WRR showed higher inhibition rates and a higher total glucosinolate content than LRR. The glucosinolate contents of the radishes were analyzed by the Pd-quicktest method, showing that WRR contained more glucosinolate than LRR in both the trunk and root. In conclusion, these results indicate that wild race radish could be used for the quality improvement of radishes.

• Journal of Life Science
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• v.17 no.10
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• pp.1361-1367
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• 2007

I investigated the effect of KT5720, an inhibitor of protein kinase A, on the vesicular stomatitis virus (VSV) infection in BHK-21cell cultures. The virus inducted cytopathic effect (CPE) was almost completely suppressed by KT5720 at 5uM. The inhibitor, however, did not affect replication of the virus nor the synthesis of viral macromolecules. KT5720, did not block the cytoskeletal disruption, while the cell rounding was suppressed. And, the KT5720-sensitive function may be involved in developing the VSV-induced CPE, but not essential for the virus replications.

• Journal of Periodontal and Implant Science
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• v.25 no.1
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• pp.45-55
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• 1995

치주질환의 치유에 있어서 치주인대 세포의 증식과 분화는 매우 중요하다. 몇몇 학자들에 의해 치주인대 세포의 증식과 분화에 영향을 주는 platelet derived growth나 fibronectin과 같은 growth factor에 대한 연구가 있었다. 이 연구는 홍삼 총사포닌이 치주인대 세포에 미치는 세포독성과 세포의 성장 및 분화에 미치는 영향을 규명하고자 사람의 치주인대 세포를 분리, 배양하여 실험하였다. 총사포닌이 치주인대 세포에 미치는 세포독성을 특정하기 위해 여러가지 농도의 총사포닌을 세포배양액에 첨가하여 1주일 배양후의 결과와 단일 농도($1{\mu}g/ml$})하에서의 세포 성장을 혈구계산반을 사용하여 관찰하였다. 치주인대 세포가 조골세포양세포로의 분화과정에 사포닌이 영향을 미치는 것을 관찰하기 위해 개개의 총사포닌 농도)0.1,1,$10{\mu}g/ml}$)를 세포배양액에 첨가하여 배양하였다. 치주인대 세포가 조골세포의 표현형으로 분화되는데 미치는 총사포닌의 영향을 알아보기 위하여 총사포닌의 단일 농도($1{\mu}g/ml$)하에서 $50{\mu}g/ml$ ascorbic acid와 10mM ${\beta}-glycerophosphate$를 배양액에 첨가하여 배양후 von Kossa's staining을 시행하여 생성된 골결절을 관찰하였다. 이상의 실험에서 얻어진 결과는 아래와 같다. 1. 각각의 농도를 투여한 결과, $1{\mu}g/ml$의 총사포닌에 의해서 세포독성이 유의성있게 증가하였다. 2. 0.01,0.01,1,$10{\mu}g/ml$의 총사포닌을 세포배양액에 첨가한 다음 7일 후의 고나찰시 cell viability가 실험농도 모두에서 유의성있게 증가하였다. 3. 0.1,1,10,$100{\mu}g/ml$의 농도에서 유외성있는 세포 증식이 있었다. 4. $1{\mu}g/ml$의 총사포닌을 세포배양액에 첨가한 다음 1,3,5,7,9일의 관찰시 시간경과에 따라 유의성있는 세포 증식이 있었다. 5. $10{\mu}g/ml$의 총사포닌을 세포배양액에 첨가시 ALP activity가 대조군에 비해 유외성있게 증가하였다. 6. $1{\mu}g/ml$의 총사포닌으로 배양된 치주인대 세포내에서 ALP positive cell이 관찰되었다. 7. $1{\mu}g/ml$의 총사포닌으로 배양된 치주인대 세포내에서 골결절 형성이 관찰되었다.

• Tuberculosis and Respiratory Diseases
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• v.50 no.5
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• pp.549-556
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• 2001

Background : IGF-I is an important mitogen in many types of malignancies. Tumors also express many IGF binding proteins, which modulate IGF action. The propose of this study was to evaluate the effect of IGF-I and IGFBP on cell proliferation in mouse lung cancer cells (3LL). Methods : The cellular proliferation of 3LL with the treatment of growth factors was evaluated using MTT assay. Western ligand blot was performed in order to determine whether 3LL cells secrete IGFBPs and we evaluated the effect of IGFBP on cellular proliferation. Results : The treatment of 3LL cells with IGF-I increased cellular proliferation in a serum free media. Western ligand blot of conditioned medium of 3LL with $^{125}I$-IGF-I demonstrated one single major band with an estimated molecular mass of 24 kDa. This band was identified as IGFBP-4 with immunoblot analysis using antisera. The addition of anti-IGFBP-4 antibody to abrogate the effect of IGFBP-4 resulted in increased cellular proliferation suggesting that IGFBP-4 inhibits cell growth. Conclusion : IGF-I increases cellular proliferation, however the secreted IGFBP-4 has an inhibitory function on cell growth in 3LL. These findings suggest that IGF-I and IGFBP are involved in the cell proliferation.

• Journal of Chest Surgery
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• v.43 no.4
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• pp.447-449
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• 2010

Langerhans cell Histiocytosis can present as a single or multiple lesion and can affect one or several organ systems. A 41-year-old woman with a history of multisystemic Langerhans cell Histiocytosis invading lung and thyroid was admitted with left-sided spontaneous pneumothorax. Here we report a case of uncommon pulmonary Langerhans-cell Histiocytosis presenting with spontaneous pneumothorax as a multisystemic Langerhans cell Histiocytosis.

• Journal of the korean academy of Pediatric Dentistry
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• v.34 no.3
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• pp.438-446
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• 2007

Viable cells of periodontal ligament would be an important factor for the successful replantation of an avulsed tooth. Therefore, it is critical to choose the storage medium for the preservation of traumatically avulsed teeth. Growth factors and hormones could be considered for the therapeutic application of the maintenance of viable periodontal ligament fibroblasts (PDLFs). Epidermal growth factor (EGF) has been suggested as an important player for the regeneration and wound healing process on other tissues. Therefore, EGF was evaluated for the therapeutic application on avulsed teeth. In addition, the synergic effect of EGF with tri-iodothyronine (T3) and heparin-binding epidermal growth factor-like growth factor (HB-EGF). The cell proliferation of PDLFs was determined by MTT assay and increased dose-dependently up to 10 ng/ml in the presence of EGF. Maximum cellular growth was shown at the concentration of 10 ng/ml EGF. Also, EGF promoted the wound healing of PDLFs examined by in vitro wound healing assay. Combined effects of EGF with T3 or HB-EGF on the proliferation of PDLFs were also studied. Interestingly, EGF showed the synergic effect on the proliferation of PDLFs with T3 and HB-EGF. To find out the mechanism of the synergic effect of EGF and T3, the effect of T3 on the expression of endogenous EGF receptor was determined by RT-PCR. The result was that T3 enhanced the expression of EGF receptor in PDLFs. It suggested that EGF might be a good choice for a therapeutic application, which can be used as combination with T3 and HB-EGF.

• The korean journal of orthodontics
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• v.25 no.3 s.50
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• pp.333-339
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• 1995

The hormonally active vitamin D metabolite, 1,25-dihydroxy vitamin $D_3[1.25-(OH)_2D_3]$ is one of the several humoral factors that may regulate osteoblast differentiation. The purpose of this study was to evaluate the effects of $1,25-(OH)_2D_3$ on the PDL cells. Human PDL cells were prepared from the first premolar tooth extracted for the orthodontic treatment and they were incubated in the environment of $37^{\circ}C,\;5\%\;CO_2\;and\;95\%$ humidity. $[{^3}H]$-thymidine incorporation as a measure of proliferation potential and alkaline phosphatase activity were evaluated at 10nM, 100nM $1,25-(OH)_2D_3$. The observed results were as follows. 1. $1,25-(OH)_2D_3$ was significantly enhanced $[{^3}H]$-thymidine incorporation at 100nM, But did not affect by 10nM. 2. $1,25-(OH)_2D_3$ was significantly increased alkaline phosphatase activity at 1 day and 6 days in a dose-dependent manner.