• Journal of Plant Biotechnology
• /
• v.36 no.1
• /
• pp.23-29
• /
• 2009

We have previously isolated a CCCH type zinc-finger protein gene, OsZF2 (Oryza sativa Zinc Finger 2), from the cold-treated rice cDNA library. To investigate the potential role of OsZF2, transgenic rice lines over-expressing OsZF2 under the control of CaMV 35S promoter have been developed through Agrobacterium-mediated transformation. Elevated level of OsZF2 transcripts was confirmed by RNA gel blot analysis in transgenic rice. Under the 100 mM NaCl condition, the transgenic rice showed significantly enhanced growth rate in terms of shoot length and fresh weight, implicating that OsZF2 is likely to be involved in salt response of rice. In the field condition, however, the transgenic rice showed a dwarf phenotype and flowering time was delayed. Genome expression profiling analysis of transgenic plants using the 20K NSF rice oligonucleotide array revealed many up-regulated genes related to stress responses and signaling pathways such as chaperone protein dnaJ 72, salt stress-induced protein, PR protein, disease resistance proteins RPM1 and Cf2/Cf5 disease resistance protein, carbohydrate/ sugar transporter, OsWAK kinase, brassinosteroid LRR receptor kinase, and jasmonate O-methyltransferase. These data suggest that the CCCH type zinc-finger protein OsZF2 is a upstream transcriptional factor regulating growth and stress responsiveness of rice.

• Korean Journal of Microbiology
• /
• v.49 no.4
• /
• pp.419-423
• /
• 2013

Three genes, nsdD, nsdC, and veA are known to be necessary for positive regulating sexual development of Aspergillus nidulans. Since the mutants of those genes hardly form fruiting bodies in heterokaryons constructed by cross between two of them, it is difficult to isolate double mutants. In this work, double mutants of ${\Delta}nsdD$ ${\Delta}veA$ and ${\Delta}nsdD$ ${\Delta}nsdC$ were isolated using the characteristic of the nsdD deletion mutant that it could develop mature cleistothecia in hypoxic and low temperature culture condition. According to the phenotypes of double mutants, the nsdD gene controls the apical growth independently with veA or nsdC. Deletion of veA or nsdC was epistatic to nsdD deletion for pigment production. Conidia formation in submerged culture with lactose as sole carbon source was observed in ${\Delta}nsdD$ ${\Delta}nsdC$ double mutant implicating it to be unique phenotype of nsdC deletion.

• Journal of Animal Science and Technology
• /
• v.45 no.2
• /
• pp.327-334
• /
• 2003

This study was carried out to make early prediction of carcass yield grade. Sixty six Hanwoo steers were measured for back fat thickness, longissimus muscle area and body weight at 18, 21 and 24 months of age by ultrasound. Carcass evaluation was done after ultrasound measurement at 24 month of age. Ultrasonic yield grade at 18, 21 and 24 month of age were predicted by regression and decision tree methods. Classifying by carcass yield grade, ultrasonic back fat thickness at 18, 21 and 24 months of age was significantly different in each carcass yield grade (p<0.05). The prediction accuracy of carcass yield grade by regression method was 78.8% at 18 months, 86.4% at 21 months and 90.9% at 24 months of age. By using the decision tree method for carcass yield grade, 78.8%, 89.4% and 89.4% of prediction accuracy were obtained at 18, 21 and 24 months of age, respectively.

• Journal of Animal Science and Technology
• /
• v.45 no.5
• /
• pp.679-688
• /
• 2003

The leptin receptor gene(LEPR) produces a high affinity receptor that mediates the regulation of the leptin gene. Leptin secreted from adipose tissue plays an important role in regulating feed intake and energy balance. In this study, a microsatellite marker within LEPR was selected and genotyped for the F2 population composed of 354 individuals from an intercross between Korean Native boars and Landrace sows. Totally, six alleles (255, 259, 261, 263, 265 and 267bp) and nineteen genotypes were detected in the population, of which the CE (261/265), CC (261/261) and EE (265/265) types were observed by 20.0%, 10.1% and 9.6%, respectively. Relationships between their genotypes and economic traits were analyzed. We found specific genotypes associated with economic traits such as body weight at 12 weeks of age/body fat including abdominal and trimmed fat/shear force (P〈0.001), body weight of 30 weeks of age (P〈0.01) and body weight of 3 weeks of age/back fat thickness (P〈0.05). The DD (263/263) and DF (263/267) types were associated with body weight at 3, 5, 12 and 30 weeks of age. The DF (263/267) type showed a highly significant effect on back fat thickness and body fat including abdominal and trimmed fat. The DF (263/267) type showed positive effect on shear force, whereas the BB (259/259) and DD (263/263) types negatively affected on tenderness.

• Journal of Animal Science and Technology
• /
• v.55 no.5
• /
• pp.351-358
• /
• 2013

This study was conducted to estimate the correlation structure between body size traits, carcass traits, and primal cuts in Hanwoo steers. Hanwoo progeny test data (body weight and body measurements) were collected from 2008 to 2010 from a total of 882 steers at the Hanwoo Improvement Main Center (NACF). Carcass traits considered were cold carcass weight, eye muscle area, backfat thickness, and marbling scores evaluated at the time of carcass grading. Correlation analyses were performed with observed scales of the traits and with residuals considering fixed environmental effects in generalized linear models. The correlation coefficient estimated between loin weight and chest girth was high at 0.74. The shank negatively correlated with pelvic width (-0.23) and hipbone width (-0.27). In addition, rib weight and chest girth was highly correlated (0.80). The correlation between carcass weight and chest girth was 0.86 in observed scale. Residual correlation between these traits was estimated at 0.65. Correlation between carcass weight and loin was 0.87 in the observed scale. Residual correlation was estimated at 0.83. The correlation coefficient estimated between shank weight and brisket and flank weight was negative at -0.69. Chest girth had a high correlation between primal cuts weight and body measurements. Thus, we believe that these results will provide a greater understanding on the relationship of primal cuts and other phenotypes, thus enabling valid production models for Hanwoo steers.

• Journal of Animal Environmental Science
• /
• v.12 no.1
• /
• pp.13-20
• /
• 2006

This experiment was carried out to determine the effect of breed and sex on behavioral characteristics and economic traits of performance-tested pigs. Totally 32 tested pigs in 4 different breeds were assigned for behavioral observation. Behavior was recorded for 4 hours(each 2 hours, before and after none) of tested pigs in each tested pen. The average daily gain, age at 90 kg and feed efficiency on the basis of the performance data were collected from 8,477 performance-tested pigs in which pure breeds of Duroc, Yorkshire, Landrace and Berkshire at the Korea Swine Testing Station. The results obtained in this study are summarized as follows; The effect of breed was statistically significant for all traits studied. Average daily gain were the highest(P<0.05) in Duroc and ages at 90 kg were the highest(P<0.05) in Berkshire. In feed efficiency of male, Landrace and Yorkshire were the most efficient. The average daily gain and feed efficiency were superior to those of female, however, the age at 90 kg was not different. Overally, females were superior to males in various traits examined. Berkshire breed had more proportion of time spent in ventral tying and sitting whereas the proportion of time spent in walking, drinking and eating was less than that of other breeds. The male pigs showed more time spent in standing and social behavior.

• Journal of Life Science
• /
• v.33 no.12
• /
• pp.987-994
• /
• 2023

This study examined the associations between the genotypes of the galactose mutarotase (GALM) gene and carcass traits in the Hanwoo population of Jeju Island, South Korea. The GALM genotypes were determined by the 14-bp (5'-GGTCTAATGACCAG-3') insertion/deletion (InDel) polymorphisms of the 3'-untranslated region (UTR). All three genotypes (LL, LS, and SS) were found in the Hanwoo steer population. The association analysis showed significant associations between genotypes and several carcass traits, including traits related to intramuscular fat content, such as meat quality, marbling score, and backfat thickness (p<0.05). Animals harboring the SS genotype showed not only higher levels of intramuscular fat content but also lower levels of backfat thickness than animals harboring the LL and LS genotypes. On the other hand, no significant associations were found between the GALM genotypes and carcass weight, eye muscle area, meat color, or fat color (p>0.05). Deleting the 14-bp segment in the 3'-UTR resulted in the modification of the secondary structure of RNA and appeared to affect gene expression by interfering with the binding ability of GALM mRNA with RNA-binding proteins and microRNAs. These results suggest that the 14-bp InDel polymorphism in the 3'-UTR region of the GALM gene affects cattle growth traits and carcass quality through galactose metabolism-mediated fat accumulation in muscle and backfat tissues.

• Food Science of Animal Resources
• /
• v.28 no.2
• /
• pp.181-186
• /
• 2008

This study was conducted to investigate the effects of supplementation with transgenic Bacillus subtilis secreting chitinase on the growth performance, nutrient digestibility, blood characteristics and carcass traits in finishing pigs. A total of sixty-four pigs ($50.82{\pm}0.82kg$, average initial body weight) were assessed over a period of 84 days. Dietary treatments included: 1) CON (basal diet without antibiotics), 2) AD (basal diet + 0.1% Virginiamycin), 3) CD0.5 (basal diet + 0.5% transgenic B. subtilis), 4) CD1.0 (basal diet + 1.0% transgenic B. subtilis). Each dietary treatment had 4 replicates of 4 pigs per pen in a randomized complete block design. In terms of growth performance, the ADG (average daily gain) and gain/feed ratio were significantly increased with the CD1.0 diet compared to the AD diet during weeks 0-4 (p<0.05). During weeks 4-8, the ADG was significantly increased with the AD diet compared to the CON and CD0.5 diets (p<0.05). The ADFI was also significantly increased with the AD diet compared to the other diets (p<0.05). During weeks $8{\sim}12$, the ADFI was significantly increased with the CON diet compared to the other diets (p<0.05), and the gain/feed ratio was significantly increased with the CD0.5 and CD1.0 diets compared to the AD diet (p<0.05). Over the entire 84 day test period, the ADFI was significantly increased with the AD diet compared to the CD0.5 and CD1.0 diets (p<0.05). The gain/feed ratio was significantly increased with the CD0.5 and CD1.0 diets compared to the CON diet (p<0.05). In terms of meat color, the L value was significantly increased with the CD0.5 diet compared to the CON and AD diets (p<0.05), and the a value was significantly increased with the CON diet compared to the other diets (p<0.05). In terms of sensory evaluation, meat color was significantly improved with the CON, CD0.5 and CD1.0 diets compared to the AD diet (p<0.05). Marbling was significantly increased with the CON diet compared to the other diets (p<0.05). Firmness was significantly increased with the CD0.5 diet compared to the AD diet (p<0.05). In conclusion, supplementation with transformed B. subtilis secreting chitinase improved gain/feed ratios and influenced meat color. Thus, we suggest that transformed B. subtilis secreting chitinase can partially substitute for antibiotics.

• Korean Journal of Ichthyology
• /
• v.7 no.2
• /
• pp.171-176
• /
• 1995

Age and growth of Dictyosoma burgeri, sampled in the costal waters off Sanyang, Tongyeong, Ky$\breve{o}$ngnam during December 1992∼November 1993, were studied by otolith reading. Examination of outer margins of the otolith showed that the opaque zone as annulus was formed once a year with being mainly configured in November. Spawning period was estimated from January to March, with a peak in Feburary. Growth of the fish was expressed by the von Bertalanffy's equation as $L_t=23.5977(1-e^{-0.2542(t+2.1302)})$, $W_t=64.5892(1-e^{-0.2542(t+2.1302)})^{2.3002}$, where $L_t$ is total length in ㎝, $W_t$ is total weight in g and t is age.

• Korean Journal of Poultry Science
• /
• v.43 no.4
• /
• pp.273-279
• /
• 2016

Recently, it was reported that certain polymorphisms in the growth hormone secretagogue receptor gene (GHSR) are associated with the growth of chickens. However, the correlation between GHSR polymorphisms and economic traits has not been investigated in Korean native chickens (KNCs). Therefore, the objective of this study was to confirm the suitability of the GHSR gene as a candidate for genomic selection and identify a genetic marker for KNCs. A total of 220 KNCs from six breeds raised at the National Institute of Animal Science were genotyped for the c.739+726 SNP in the GHSR gene using polymerase chain reaction- restriction fragment length polymorphism (PCR-RFLP), and the sequence for a subset of 30 birds was analyzed using direct sequencing. The association between the SNP genotypes and the economic traits of the KNCs was analyzed using the statistical package for the social science (SPSS) software program. The association analysis between the c.739+726T>C SNP and economic traits revealed that the SNP was significantly associated with body weight at 150 and 270 days (BW150 and BW270, respectively) in all KNCs (p<0.01), BW150 in KNC (Gary) (p<0.05), and egg production number in KNC (White, p<0.05). In addition, the SNPs discovered using direct sequencing (513A>G, 517A>T) had a significant effect on the body weight and egg production traits (p<0.05). In conclusion, these results might be useful as a basis for studies on the improvement of KNC breeds. Furthermore, these results suggest that the SNPs (c.739+726T>C, 513A>G, and 517A>T) located in the GHSR gene could be useful molecular genetic markers for KNCs.