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수율 계수와 목적 함수의 최적 유가배양에 대한 영향

  • 박성렬;최차용
    • Proceedings of the Korean Society for Applied Microbiology Conference
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    • 1979.10a
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    • pp.244.3-245
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    • 1979
  • 1. 수율계수 함수의 영향 : 유가 배양에 있어서 수율 계수가 제한 기질 농도의 함수일 경우에 대한 연구를 수행하였다. 사용한 수율 계수 함수는 기질 농도에 대하여 선형 함수였으며 목적함수는 중간 대사 물질의 생산량이었다. 1-가) 수율 계수 함수의 기울기가 음의 값으로 적어짐에 따라 제한 기질의 공급속도가 증가되었다. 1-나) 일반적으로 초기의 얼마 동안은 회분 배양에서와 같이 공급 속도가 거의 없었으며 그후 전환점 이후에 가서야 공급 속도가 증가하였다. 1-다) 전환점 이후에서도 처음에는 대부분의 경우에 공급속도가 음의 값으로 부터 시작하여 증가하기 시작하였다. 1-라) 어떤 경우에는 배양 초기부터 공급 속도가 양의 값으로부터 시작하였으나 곧 감소하였다가 다시 증가하는 비 단조형 변화를 보였다. 1-마) 전환점 이후에서 공급 속도가 증가하는 형태에서도 처음에는 선형 증가를 보이다가 나중에 지수적 증가를 보였다. 1-바) 위와 같은 공급 속도의 변화에 따른 발효조 내의 제한 기질 농도의 변화는 초기에는 가능한 최대치를 유지하다가 전환점 이후에는 갑자기 감소하는 형태를 취하였다. 2. 목적 함수의 영향: 목적 함수가 균체량과 생성 중간 대사물질 량의 선형 결합일 때 대하여 연구하였다. 선형 결합 계수로는 균체량과 생성대사 물질의 상대적인 값어치를 취하였다. 2-가) 허용 최대 균체 농도가 제한되어 있는 상황 하에서 생성 대사 물질의 상대적인 값이 증가 할수록 목적 함수가 증가하였다. 2-나)생성대사 물질의 상대적인 값이 증가할수록 전환점의 위치가 줄어들었다. 2-다) 생성대사 물질 상대적 값의 단위 증가에 대한 목적 함수는 균체량을 감안하지 않은 야마네박사의 결과에 수렴하였다. 2-라) 공급 속도는 상대 값이 커질수록 줄어들었다. 2-마) 균체량의 시간에 대한 곡선은 상대값이 증가할수록 감소하고 전형적인 분기점 형태의 변화를 보였다.변이 뚜렷하여 이로 인한 외임파강내의 염증성병변이 뚜렷이 나타나 있으며 와우관의 특히 기저회전에서의 유모세포의 손실이 심한 것으로 보아 중이염으로 인한 골도의 고음역에서의 손실이 발생함을 알 수 있다.A group), vinclozolin, procymidone, tetradifon cypermethrin, 그리고 fenvalerate(B group)에서는 70% 이상의 회수율을 얻었고, 20%의 ethyl acetate/hexane을 사용하여 분석한 경우는 5%나 10%의 ethyl acetate/hexane을 사용하여 분석했을 때보다 좋은 결과를 나타내어 전체 16종의 농약 중 14종에서 75% 이상의 회수율을 얻었으나, alachlor와 bifenthrin은 매우 낮은 회수율을 보여서 ethyl acetate와 hexane의 혼합용매가 이들 두 농약성분의 분석에는 적합하지 않은 용출 용매임을 알 수 있었다.NO_2의$ 급성 중독증상으로서는 눈, 코를 강하게 자극하고 폐충혈, 폐수종, 기관지염, 폐염 등을 일으킨다. 만성 중독시에는 만성폐섬유와 및 폐수종을 일으킨다. (4) $오존(O_3)$ Ozone은 자동차 배기가스에서 나오는 $NO_2$ gas 및 탄화수소와 작용하여, PAN이라는 자극성 물질을 생성시키는 광학적 Smog의 주요소로 알려져 있다. 자극적인 냄새가 있음으로 불쾌감을 주고 비, 인후점막의 전조감과 두통이 오며 폐기능을 저하시키며 더욱 진행되면 폐충혈, 폐수종 등을 일으킨다 (5) Smog에 의한 건강피해 대표적인 것이 1952.12.5~12.8까지 4일간 영국 Lon-don에서 계속된 Smog사건이며, 이 사건으로 말미암아 호흡기질환 사망율이 사건전보다 사건기간 중 혹은 사건후에 5~10배의 증가율을 보였다. 이때 Smog의 주원인은 연료의 불완전 연소에 의한 연기와 이때 발생

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Growth Characteristics and Productivity of New Hairy Vetch(Vicia villosa Roth) Early-Maturing "Cold Green" and Medium-Late Maturing "Cheong Pa" (헤어리베치 신품종 조생종 콜드그린과 중만생종 청파의 생육특성 및 수량성)

  • Shin, Chung-Nam;Ko, Ki-Hwan;Kim, Jong-Tak;Lee, Joung-Kyong;Seo, Sung;Seong, Byung-Ryul;Choi, Gi-Jun;Kim, Jong-Duk;Oh, Myung-Gon
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.27 no.4
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    • pp.257-262
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    • 2007
  • The objective of this research was to develop a high yielding, early and medium-late flowering new hairy vetch(Vicia villosa Roth) varieties derived from an accession in Korea. Most vigorous early and medium-late flowering hairy vetch lines were selected and crossed by open pollination and their seeds were bulked and plants were reselected at Seongju in the Keongbuk and at Sacheon in the Keongnam. A performance trial was conducted to evaluate agronomic characteristics, forage quality and dry matter(DM) yield of new hairy vetch early-maturing 'Cold green' and medium-late maturing variety 'Cheong pa' at Seongju and Sacheon. 'Cold green' and 'Cheong pa' are hair but 'Haymaker plus' and 'Latigo' are nearly hairless. 'Cold green' has purple flowers while 'Cheong pa' has violet. The cold tolerance of 'Cold green,' 'Cheong pa' and 'Latigo' was higher than 'Haymaker plus' at Seong-ju, Keongbuk province, in inland region but there were a little differences at Sacheon, Keongnam province in southern coast region. Fifty percent-flowering dates of 'Cold green' and 'Haymaker plus' had earlier than others and 'Cheong pa' was earlier than 'Latigo'. The DM yield of 'Cold green' was higher than that of others, but 'Cheong pa' and 'Haymaker plus' had higher than 'Latigo' at Seong-ju(P<0.05). In Sacheon, The DM yield of 'Cold green' and 'Haymaker plus' was higher than others and 'Cheong pa' had higher than 'Latigo'.

Screening of Medicinal Plants with Antifungal Activity on Major Seedborne Disease (주요종자전염병 억제를 위한 항균성 약용식물탐색)

  • Paik, Su-Bong;Chung, Il-Min;Doh, Eun-Soo
    • Korean Journal of Medicinal Crop Science
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    • v.6 no.4
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    • pp.277-285
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    • 1998
  • Antifungal activity on major seedborne diasease of crops was screened by the treatment of the extracts from 50 medicinal plants in vitro and in vivo. The extracts of garlic and taxus, Rheum undulatum, Achiranthes japonica, Glycyrrhiza uralensis, Oenothera lamar kiana treated with the blotting filter paper and water agar methods inhibited the growth of Pyricularia oryzae, Alternaria sesamicola, Colletotrichum gloeosporioides, and Alternaria brassicicola among the tested plants. Antifungal activities on infected seeds by soaking methods were shown even at the dilution of the extracts by 10 times. The activity was the highest in soaking seeds at $25^{\circ}C$ for 24 hours. The effect of plant extract on seed germination was not significant as compared with untreated seed. However, early growth of seedling was increased by the treatment of extracts. The extract of taxus slightly inhibited the seed germination of radish and chinese cabbage but those of Achirunthes japonica, Glycyrrhiza uralensis and Oenthfera lamarkiana showed severe damage on the seed germination and early growth of seedling.

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Agronomic Characteristics, Forage Quality, and Dry Matter Yield of Forage Winter Rye Cultivars (사료용 호밀 보급품종의 주요 생육특성과 수량성)

  • Sung, B.R.;Rim, Y.W.;Kim, M.J.;Kim, K.Y.;Choi, G.J.;Lim, Y.C.;Lim, G.B.;Park, G.J.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.4
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    • pp.301-308
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    • 2004
  • This experiment was carried out to classify heading date of forage rye cultivars, and study agronomic characteristics, dry matter yield per ha and forage quality of winter rye cultivars in Suwon for 2 year from 2002 to 2003. The earliest cultivars among forage winter rye cultivars were Olhomil and Vitagrazer, which the heading date was April 21. The latest cultivar was Danko, which was May 5. Koolgrazer was April 26 and Homil 22 was April 30, respectively. Therefore, we classified the cultivars into three groups according to their heading date. The earliest group includes Olhomil, Vitagrazer, Paldanghomil, the middle group includes 5 cultivars which are Koolgrazer, Barrgazer, Maton, Wintergrazer and Homil22, and the latest group contains Humbolt and Danko. The number of tiller per $m^{2}$ of most cultivars were more than 1,000. The cultivar which has lowest tiller was Humbolt, which dry matter yield was the largest, and its tiller was 789. The lodging happened after each precipitation. Danko was the cultivar that has lowest lodging frequency. There has been little disease and insect and the difference in the quality of each cultivar. The yield of dry matters per ha of Humbolt was increased $123\%$, Vita grazer $121\%$, Maton $113\%$ comparing the standard cultivar, Kool grazer of 12,409kg, and Homil 22 was almost the same with the standard cultivar.

Production of Transgenic Orchardgrass Overexpressing a Thermotolerant Gene, DgP23 (내열성 유전자 DgP23을 도입한 형질전환 오차드그라스의 생산)

  • Kim Ki-Yong;Jang Yo-Soon;Park Geun Je;Choi Gi Jun;Seong Byung Ryul;Seo Sung;Cha Joon-Yung;Son Daeyong
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.267-274
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    • 2005
  • To develop transgenic orchardgrass (Dactylis glomerata L.) resistant to high temperature, a thermptolerance gene, DgP23, was introduced into orchardgrass using Agrobacterium - mediated transformation method. PCR and Southern blot analyses using genomic DNA showed specific DNA band on agarose gel and hybridization signal on X- ray film in transgenic orchardgrass harboring the recombinant DgP23 gene, but not in the wild type and empty vector control plants. RT-PCR and Southern blot analyses using total RNA also showed specific DNA band and hybridization signal. Transgenic orchardgrass did not showed ny morphological aberration both in the green house and field cultivation. Thermotolerance of transgenic plants was not detected in laboratory test. but may detected in field test.

Growth Characters and Productivity of Italian Ryegrass (Lolium multiflorum L.) New Variety 'Hwasan 104' (이탈리안 라이그라스 신품종 '화산 104호'의 생육특성과 수량성)

  • Choi Gi Jun;Rim Yong Woo;Sung Byung Ryul;Lim Young Chul;Kim Meing Jooung;Kim Ki-Yong;Park Geun Je;Park Nam Keon;Hong Youn Ki;Kim Sang Rok
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.25 no.4
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    • pp.275-280
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    • 2005
  • This experiment was carried out to breed the cold-tolerant variety of Italian ryegrass (Lolium multiflorum Lam.) in Grassland and Forage Crops Division, National Livestock Research Institute, RDA, Suwon from 1997 to 2002. Hwasan 104 as a tetraploid variety was dark green in leaf color and had semi-prostrate and medium growth habit in late autumn and early spring, respectively. Hwasan 104 was 19th May in heading date as a late-heading variety. Especially, Hwasan 104 was broader in flag leaf and longer 7 cm in plant length, and thicker in stem width than those of control variety, Hwasan 101. Cold tolerance of Hwasan 104 was better than that of Hwasan 101 registered as a cold-tolerant variety. Dry matter (DM) yield of Hwasan 104 was $5\%$ more than DM 9,348 kg/ha of Hwasan 101. In vitro dry matter digestibility and total digestible nutrient of Hwasan 104 were 74.3 and $63.1\%$ which are 3.3 and $1.5\%$ lower than those of Hwasan 101, respectively. Acid detergent fiber and neutral detergent fiber of Hwasan 104 were 32.6 and $55.8\%$ which are 1.9 and $2.4\%$ higher than those of Hwasan 101, respectively.

Effects of Drill Widths and Nitrogen Application Levels in Early Spring on the Growth Characteristics and Seed Productivity of Italian Ryegrass (Lolium multifloum Lam.) (휴폭 및 이른 봄 질소시용이 이탈리안 라이그라스의 생육특성과 종자수량에 미치는 영향)

  • 최기준;정의수;임용우;임영철;김기용;성병렬;박근제
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.3
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    • pp.221-226
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    • 2002
  • The effects of drill widths and nitrogen application levels on the growth characteristics and seed productivity of Italian ryegrasss(Lolium multiflorum Lam.) were evaluated. The experimental design was a split-plot arrangement with three replications. The main-plots were three drill widths of 15, 30 and 45cm, and subplots were three nitrogen application rates of 50, 75 and 100 kg/㏊ in early spring. Among the growth characteristics, lodging tolerance appeared to be highest in the treatment including 45cm of drill width and 50kg/㏊ of nitrogen application compared to other treatments. The numbers of stem per one square meter tended to be increased more in drill width of 15cm than in that of 30 or 45cm, but other growth characteristics were similar among treatments. In seed yield components, the numbers of spike per one square meter were getting increased according as drill width was narrower, but spike length and 1,000 seed weight were longer and heavier. respectively. The rate of ripened pains was highest in treatment including 30cm of drill width and 50kg/㏊ of nitrogen application. Maximum seed yield was also obtained in same treatment.

Pattern Analysis for Urban Spatial Distribution of Traffic Accidents in Jinju (진주시 교통사고의 도시공간분포패턴 분석)

  • Sung, Byeong Jun;Yoo, Hwan Hee
    • Journal of Korean Society for Geospatial Information Science
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    • v.22 no.3
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    • pp.99-105
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    • 2014
  • Since traffic accidents account for the highest proportion of the artificial disasters which occur in urban areas along with fire, more scientific an analysis on the causes of traffic accidents and various prevention measures against traffic accidents are needed. In this study, the research selected Jinju-si, which belongs to local small and medium-sized cities as a research target to analyze the characteristics of temporal and spacial distribution of traffic accidents by associating the data of traffic accidents, occurred in 2013 with the causes of traffic accidents and location information that includes occurrence time and seasonal features. It subsequently examines the spatial correlation between traffic accidents and the characteristics of urban space development according to the plans of land using. As a result, the characteristics of accident distribution according to the types of accidents reveal that side right-angle collisions (car versus car) and pedestrian-crossing accident (car versus man) showed the highest clustering in the density analysis and average nearest neighbor analysis. In particular, traffic accidents occurred the most on roads which connect urban central commercial areas, high-density residential areas, and industrial areas. In addition, human damage in damage conditions, clear day in weather condition, dry condition in the road condition, and three-way intersection in the road way showed the highest clustering.

Effects of Sex Education Program related to the Unit of Reproduction and Development on Middle School Boys' Sex-related Knowledge and Attitude towards Sex (생식과 발생 단원과 연계한 성교육 프로그램이 중학교 남학생들의 성지식 및 성태도에 미치는 영향)

  • Kim, Sun Young;Park, Su Bin
    • Journal of Science Education
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    • v.42 no.2
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    • pp.148-164
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    • 2018
  • This study developed the sex education program based on the 'reproduction and development' unit of the middle school science, and examined its effectiveness on middle school boys' sex-related knowledge and attitude towards sex. The sex education program consisted of six topics of the structures and functions of generative organs, the importance of life, abortion, the human generation process, venereal diseases and AIDS, and responsibility of self-determination on sexual behavior. The results indicated that the experimental group demonstrated the statistically higher scores of the knowledge on sex than the control groups' scores, especially in the subcategories of 'pregnancy and delivery' and 'sex-related health'. However, both groups did not display any statistical difference on the attitude toward sex. In the subcategory of the attitude towards sex, the experimental group showed the statistically higher scores on 'self-determination and selection' and 'a health relationship'. In addition, the Pearson correlation results indicated the strongest relationship between the attitude toward sex and 'the self-determination and selection'. These results implicated that the sex education program related to the unit of reproduction and development in the middle school science helps the middle school boys develop the better knowledge and attitude towards sex.

Growth Characteristics and Productivity of New Orchardgrass(Dactylis glomerata L.) Variety 'Kordi' (오차드그라스 신품종 '코디'의 생육특성과 수량성)

  • Rim Y. W.;Choi G. J.;Sung B. R.;Lim Y. C.;Kim M. J.;Park G. J.;Kim K. Y.;Chung J. W.;Go S. B.
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.24 no.3
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    • pp.261-264
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    • 2004
  • New orchardgrass(Dactylis glomerata L.) variety named as 'Kordi' was developed by the National Livestock Research Institute(NLRI) in 2003. Five superior clones on selected to develope the new variety of orchardgrass, and polycrossed for synthetic seed production. Synthetic seeds were examined on the agronomic growth characteristics and forage production at Suwon from 1995 to 1998, and regional trials were conducted in Suwon, Pyungchang and Jeju from 2001 to 2003 and Icsan in 2003, respectively. 'Kordi' showed semi-erect growth habit in fall and spring, and long type in length of flag leaf and short in upper internode. Plant height of 'Kordi' was little short compared to that of standard variety, Ambassador, and heading date was delayed about 3 days as 14th May compared to Ambassador. Characters such as winter hardiness. lodging tolerance, regrowth, disease resistance of 'Kordi' were stronger or better than those of Ambassador. 'Kordi' showed $10\%$ higher dry matter yield as 15,174 kg/ha compared to Ambassador. Nutritive value was appeared to be similar in both varieties.