• Advanced Industrial SCIence
• /
• v.2 no.1
• /
• pp.8-14
• /
• 2023

This study was performed as a preliminary experiment to develop functional feed additives using by-products generated during the production of black garlic. Therefore antioxidant and immune enhancing activity of black garlic pomace were measured. As a result of measuring the antioxidant activity of black garlic pomace, it was found antioxidant activity. Nitric oxide (NO) assay was performed to test the immune enhancing activity of vegetable samples including black garlic pomace among the samples used in the experiment. As a result of the NO assay experiment, highest concentrations of black garlic pomace, aster glehni, and MIX form produced NO, which Garlic pomace (69.4%), aster glehni (35.9%), and MIX (45.3%), respectively, compared to LPS (100%). In conclusion, it is considered that black garlic pomace contains an anti-inflammatory effect, and if the optimal mixing ratio of black garlic pomace and aster glehni is selected, it will be of sufficient value as a feed additive containing an anti-inflammatory effect.

• Microbiology and Biotechnology Letters
• /
• v.38 no.4
• /
• pp.434-441
• /
• 2010

The Plants and their extracts containing polyphenol have been shown to be associated with decreased the cause of aging and variety of disease such as reaction oxygen species (ROS) and reactive nitrogen species (RNS) in several recent studies. We conducted to investigate whether the extracts and fractionation isolated from Aster glehni Fr. Schm. has an inhibitory effect association with oxidation or inflammation. The Aster glehni Fr. Schm. 70% aq. MeOH was fractioned according to polarity with n-hexane layer, EtOAc layer, n-BuOH layer and water layer. The electron donating ability of EtOAc, n-BuOH solvent fraction from Aster glehni Fr. Schm. was about 58.0%, 46.4% at $50\;{\mu}g/mL$, respectively. The superoxide anion radical inhibitory effect of EtOAc extracts was about 64.65% at $50\;{\mu}g/mL$, and n-BuOH extracts was 35.66% at $50\;{\mu}g/mL$. EtOAc layer to the inhibition activity of hyaluronidase and lipoxygenase were inhibited about 24.37%, 29.5% at $5\;{\mu}g/mL$. In the anti-inflammation effect of EtOAc layer inhibited the generation of nitric oxide. also, these results showed that EtOAc extract inhibited 81.5% at $50\;{\mu}g/mL$ on the expressions of iNOS protein in Raw264.7 cell line.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2018.04a
• /
• pp.53-53
• /
• 2018

전라북도 동부권 지역은 중산악지형으로 표고 100~500m 산지가 69%, 500~1,000m가 26%로 이루어져 있어, 산채를 재배하기에 유리한 지리적 조건을 갖고 있다. 따라서 소비자의 수요가 높은 산채류의 재배 및 이용에 대한 농업인들의 기술개발이 요구되고 있다. 이에 전라북도 준고랭지역에서 재배하기 적합한 산채작목을 선발하고자 본 시험을 실시하였다. 시험은 표고 500m 전라북도 남원시 운봉읍 허브시험장에서 노지재배, 남원시 주천면 표고 530~540m, 진안군 주천면 표고 340~350m에 위치한 농가포장에서 임간재배를 실시하였다. 대상작물은 개미취(Aster tataricus), 갯기름나물(Peucedanum japonicum), 곤달비(Ligularia stenocephala), 눈개승마(Aruncus dioicus var. kamtschaticus), 단풍취(Ainsliaea acerifolia), 산마늘(Allium microdictyon), 섬쑥부쟁이(Aster glehnii), 어수리(Heracleum moellendorffii), 우산나물(Syneilesis palmata) 등 9종을 2016년 종묘를 정식하였다. 정식 2년차에 해당지역의 5월~9월 평균기온 및 조도 등 생육환경을 조사하고, 시험작물의 출현율, 생육특성, 수량 등을 조사하였다. 조사기간 동안의 평균기온은 노지가 $20.8^{\circ}C$, 남원임간은 $20.3^{\circ}C$, 진안임간은 $20.5^{\circ}C$이었으며, 평균조도는 노지가 37,362Lux, 남원임간은 7,816Lux, 진안임간은 25,316Lux이었다. 노지에서 출현율은 개미취, 갯기름나물, 눈개승마, 산마늘, 섬쑥부쟁이, 우산나물이, 임간에서는 개미취, 곤달미, 눈개승마, 단풍취, 산마늘, 어수리, 우산나물이 높았다. 생육특성은 노지에서 개미취, 갯기름나물, 눈개승마, 섬쑥부쟁이, 임간에서는 단풍취, 산마늘, 우산나물의 생육이 좋았다. 수량은 노지에서 개미취, 갯기름나물, 곤달비, 산마늘, 섬쑥부쟁이, 어수리가 높았으며, 남원임간에서는 곤달비, 단풍취, 우산나물, 어수리가, 진안임간에서는 개미취, 갯기름나물, 산마늘, 우산나물의 수량이 많았다. 이와 더불어 준고랭지에 적합한 산채류률 선발하기 위해서는 차후 재배 지역 및 방법의 차이에 따른 산채작물의 연차별 수량성과 경제성에 관한 연구가 이루어져야 할 것으로 여겨진다.

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2020.12a
• /
• pp.71-71
• /
• 2020

산채는 사람에 의하여 개량 육성되어 논밭에서 재배하고 있는 농작물과 달리 자연 그대로 산야에서 자생하는 식물 중 식용으로 가능한 것을 말한다. 산채류의 이용형태별 생산가능시기는 3~5월의 생채 생산과 6~7월의 건채 생산으로 소비자의 기호에 부합하는 신선 생채의 소비한계는 제한되어 있기 때문에 재배유형별 생채 수량검정을 통해 수확시기 연장 및 재배품목을 다양화 시킬 필요 있다. 따라서 본 시험은 재배가능한 산채류를 수집하고 기능성 분류를 통한 꾸러미 산채 조합을 구성하였고 조합된 산채류 들에 대한 생산성 검정을 위해 해발 500m의 전라북도 농업기술원 허브산채시험장에서 2018년 5월 말 갯기름나물 등 15 종을 정식하여 각 작물의 생육특성, 수확한계기 등을 조사하였다. 기능성 분류 결과 항당뇨, 항고지혈증, 항비만, 항혈전, 항염증 총 5가지 조합을 구성하였다. 산채류별 광합성특성 조사결과 돌단풍, 개미취, 섬쑥부쟁이, 질경이, 갯기름나물, 참취의 적정광량은 600~800 μmole/m²/s이였고 눈개승마, 어수리, 참당귀는 400~500 μmole/m²/s, 곤달비, 곰취, 단풍취는 200~300 μmole/m²/s이였다. 기능성 꾸러미 산채류 지상부 생육특성 결과 갯기름나물, 질경이, 참취, 곤달비 섬쑥부쟁이 등이 생육이 우수하였다. 수량성은 섬쑥부쟁이 1,694.6kg/10a로 가장 높았으며, 갯기름나물 1,673.4kg/10a, 참취 1,521.3kg/10a, 질경이 1,398.3kg/10a, 곤달비 1,300.6kg/10a 등이 높았다. 반면 돌단풍 111.8kg/10a, 단풍취 69.5kg/10a, 우산나물 25.4kg/10a로 아주 낮은 수량성을 보였다. 산채류 출현기는 산마늘이 2월 7일로 가장 빨랐으며 대부분 산채류들이 2월 11일에서 2월 25일에 출현하였고 단풍취, 우산나물이 3월 6일로 늦은 출현기를 보였다. 기능성 꾸러미 생채 수확가능 시기는 3월 상순부터 6월 하순까지 가능하였고 수확기간은 섬쑥부쟁이 117일, 두메부추 109일, 어수리 102일, 참당귀 102일로 길었고 돌단풍, 산마늘, 눈개승마, 우산나물은 생채 수확가능 시기가 짧았다. 건나물 수확기간은 돌단풍, 눈개승마는 3월부터 6월까지 가능하였고, 대부분의 산채류들이 5월부터 10월까지고 산마늘과 우산나물은 건나물을 생산할 수 없었다. 산채류 기능성 꾸러미별 생채 조합 가능 시기는 항당뇨는 3월 중하순, 항고지혈증은 3월 중순, 항비만은 3월 하순, 항혈전 3월 하순, 항염증은 3월 중하순으로 조사되었다.

• Food Science and Preservation
• /
• v.30 no.4
• /
• pp.654-662
• /
• 2023

The Aster glehni extract has many therapeutic and medicinal values. Therefore, it is essential to set appropriate conditions for enzyme treatment to efficiently extract A. glehni. In this study, changes in the quality of A. glehni extract depending on the concentration and time of enzyme treatment was investigated to increase its effective utilization. Compared to the control, the pH of the extract of A. glehni its soluble solid content increased with the enzyme treatment. The color of the A. glehni extract changed from green-yellow to reddish-yellow with the increase in treatment duration. The fructose and sucrose contents of the extract were the highest at 7.73% and 6.78%, respectively, in the control group without the enzyme treatment. Glucose and maltose contents were 6.91% and 4.44% in the C group (3.2% enzyme concentration and 60 min for enzyme treatment), respectively. Total polyphenol content, which shows antioxidant activity, was the highest at 7.38 mg GAE/g in the E group (1.6% of enzyme concentration and 120 min for enzyme treatment). 2,2-diphenylpicrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) showed the highest radical scavenging activity in the C group (3.2% of enzyme concentration and 60 min for enzyme treatment). These results enable setting appropriate conditions of enzyme treatment in terms of enzyme concentration and time for the production of dry powders using A. glehni extract.

• The Science & Technology
• /
• no.10 s.413
• /
• pp.112-113
• /
• 2003

고온 초전도 송전선 개발/ 세계 첫 2대 2 '교환 간이식'/ "대장암 · 위암 DNA진단칩 개발"/ 울릉도 '섬쑥부쟁이' 세계적 특산식물 확인/ 대학ITRC(IT연구센터) 11곳 지정/ 대구과학축제 개최/ 제1회 전국대학생 물사랑 플래쉬 애니메이션 공모

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.45 no.11
• /
• pp.1610-1616
• /
• 2016

This study aimed to establish an optimal extraction process and high performance liquid chromatography-ultraviolet (HPLC-UV) analytical method for determination of 3,5-dicaffeoylquinic acid (3,5-DCQA) as a part of materials standardization for the development of a xanthine oxidase inhibitor as a health functional food. The quantitative determination method of 3,5-DCQA as a marker compound was optimized by HPLC analysis using a Luna RP-18 column, and the correlation coefficient for the calibration curve showed good linearity of more than 0.9999 using a gradient eluent of water (1% acetic acid) and methanol as the mobile phase at a flow rate of 1.0 mL/min and a detection wavelength of 320 nm. The HPLC-UV method was applied successfully to quantification of the marker compound (3,5-DCQA) in Aster glehni extracts after validation of the method with linearity, accuracy, and precision. Ethanolic extracts of A. glehni (AGEs) were evaluated by reflux extraction at 70 and $80^{\circ}C$ with 30, 50, 70, and 80% ethanol for 3, 4, 5, and 6 h, respectively. Among AGEs, 70% AGE at $70^{\circ}C$ showed the highest content of 3,5-DCQA of $52.59{\pm}3.45mg/100g$ A. glehni. Furthermore, AGEs were analyzed for their inhibitory activities on uric acid production by the xanthine/xanthine oxidase system. The 70% AGE at $70^{\circ}C$ showed the most potent inhibitory activity with $IC_{50}$ values of $77.01{\pm}3.13{\sim}89.96{\pm}3.08{\mu}g/mL$. The results suggest that standardization of 3,5-DCQA in AGEs using HPLC-UV analysis would be an acceptable method for the development of health functional foods.

• Food Science and Preservation
• /
• v.30 no.6
• /
• pp.1095-1106
• /
• 2023

This study investigated the anti-oxidative and anti-inflammatory effects of Aster glehni (AG) extract in RAW 264.7 cells and Caenorhabditis elegans. The total polyphenol and flavonoid contents were higher in the ethanol extracts than in the hot water extracts. As a result of measuring the moisture contents (%) and extraction yields (%) of AG and drying A. glehni for processing (DAG), 70% ethanol, which has the highest percentage of extraction yield, was selected as the final solvent. DPPH radical scavenging activity showed higher antioxidant activity of ethanol extracts of DAG than AG. The cytotoxicity assay of the AG or DAG ethanol extracts was treated at different concentrations (25, 50, and 100 ㎍/mL), and cell viability rates were higher than 80% at all concentrations. The LPS-stimulated nitric oxide (NO) production in RAW 264.7 was significantly reduced at all concentrations of AG and DAG groups. As a result of measuring the gene expression of iNOS, which induces NO production, the AG or DAG group decreased by 33% and 32%, compared with the phosphate buffer saline (PBS) group. Under inflammatory stress conditions, the survival rate of C. elegans treated with AG or DAG ethanol extract with LPS showed concentration-dependent improvement in survival rate compared with the PBS group. Considering these results, AG could potentially be developed as an antioxidant and anti-inflammatory functional food material.

• Journal of Bio-Environment Control
• /
• v.12 no.3
• /
• pp.132-138
• /
• 2003

This experiment was conducted to investigate the mass propagation system of Aster glehni FR. and to obtain the basic data for improvement of germination and seedling. The following was the results of experimentation about temperature and light conditions, and priming treatment as kinds of chemicals, their concentration and treated periods affect germination of Aster glehni FR. The germination percentage of Aster glehni FR. seed was higher in 20 and 25$^{\circ}C$ than the others, but it dropped rapidly at 30$^{\circ}C$ and didn't germinate at 35$^{\circ}C$. The first day to germination was the slowest at 15$^{\circ}C$. The germination rate of Aster glehni FR seed increased with increasing with temperatures from 15 to 25$^{\circ}C$. But the seed was rotten easily in high temperature. The germination rate was shown highest in 25$^{\circ}C$, and next was 15, 20, 30$^{\circ}C$ in order. Light treatment enhanced germination percentage, the first day to germination, germination rate and T50, but there was no significant difference. The 3 hours priming treatments had more effect on germination percentage than 30 minutes treatments as comparing averages. Aster glehni FR seeds primed in $KNO_3+K_3PO_4$ for 3 hours had most eHective on germination percentage (83.3%) and also showed shortest $T_{50{\cdot}}T_{50}$ and day of first germination was better in 30 minutes than 3 hours treatments, and most of priming treatments were better than control(non-priming seeds). While priming seeds showed shorter day of first gemination than control, there was no significant difference between 30 minutes and 3 hours treatments.

• YAKHAK HOEJI
• /
• v.48 no.1
• /
• pp.65-69
• /
• 2004

The chromatographic separation of MeOH extract of the aerial parts of Aster glehni (Compositae) led to the isolation of three sesquiterpenes, two sterols and four terpenes. Their structures were determined to be $\beta$-amyrin acetate (1), phytol (2), alismol (3), $\alpha$-tocopheryl quinone (4), $\alpha$-spinasterol (5), 10-O-methyl alismoxide (6), alismoxide (7), and 3-O-(6'-O-palmitoyl-$\beta$-D-glucosyl)-spinasta 7,22-diene (8) by physicochemical and spectroscopic methods . These compounds (1-8) were first isolated from the Aster glehni.