• Journal of Veterinary Clinics
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• v.27 no.5
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• pp.565-568
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• 2010

Sulfaquinoxaline (SQX) and trimethoprim (TMP) are chemotherapeutics that are extensively used in various animal species for the treatment or prevention of coccidia and coccidia-like parasites. Little information about the depletion kinetics of these compounds in chickens exists in the literature. In this study, a new commercial liquid concentrate of SQX in combination with TMP (100 g/l of SQX and 33.4 g/l of TMP) was administered with drinking water at a dose of 0.75 ml/l or 1.5 ml/l. The edible tissue concentrations of the drugs were determined by the validated high-performance liquid chromatography/mass spectrometric method. Residue concentrations of SQX and TMP were lower than their maximum residual limits (MRLs) in all tissues from both dose groups at 5 days after the treatment. The optimal withdrawal time of SQX/TMP combination was suggested to be over 5 days after cessation of medication in broilers.

• Journal of Food Hygiene and Safety
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• v.8 no.1
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• pp.25-35
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• 1993

This study is conducted to deyelop more conyenient simultaneous determination method by HPLC for mixed antimicrobial agents (sulfamerazine; SMR, sulfamethazine; SMT, sulfamonomethoxine; SMMX, sulfadimethoxine; SDMX, sulfaquinoxaline; SQX, furazolidone; FZ, zolene; ZOL and ethopabate; EPB in muscles of boline, pork and chicken. The drugs were extracted by dichloromethane and water. The extract, after solyent eyaporation, was partitioned in hexane/water and water/dichloromethane. The dichloromethane layer was filtrated with anhydrous $Na_5S0_4\;in\;_3G_3$ glass filter and was eyaporated to dryness. The residue was dissoIYed in mobile phase. The test solution analyzed by HPLC. The chromatograpic conditions were as follows; Column-Spheri 5 RP-8($4.6{\times}220,\;5\;{\mu}$), Wayelength-270 nm, Mobile phase-acetonitrile: 0.005 M oxalic acid (22 : 78). The ayerage recoyeries of drugs from muscles of boline, pork and chicken spiked standard solution were approximately 74~99%, 73~99% and 75~96%, respectiyely. The limits of detection were 5 ppb for SMR, SMT, SMMX, SDMX and EPB, and 8 ppb for SQX, FZ and ZOL.

• Analytical Science and Technology
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• v.20 no.1
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• pp.84-90
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• 2007

To determine levels of 11 sulfonamides for animals in food, simultaneously, a selective method of high performance liquid chromatography with UV detector has been applied. The targets were sulfachlorpyridazine (SCP), sulfadiazine (SDZ), sulfadimethoxine (SDM), sulfisoxazole (SSX), sulfamerazine (SMZ), sulfamethazine (SMT), sulfamethoxazole (SMX), sulfamethoxypyridazine (SMP), sulfamonomethoxine (SMM), sulfaquinoxaline (SQX) and sulfathiazole (STZ). Food samples were beef, pork, chicken, milk and whole egg that were collected at the main 6 cities in Korea as Seoul, Busan, Daejon, Incheon, Mokpo and Gangneung. After homogenizing food samples with sodium phosphate solution and acetonitrile, it was extracted with n-hexane. The mobile phase gradient was a mixture of 5 mM potassium phosphate (pH 3.25) and methanol with a gradient ratio from 100:0 to 30:70. The UV wavelength was 270 nm. The overall recoveries were ranged from 75% to 95% and the limit of detection was minimum 0.004 mg/kg for SMT, and 0.007 mg/kg for STZ at signal/noise > 3, respectively. As results, sulfonamide drugs were not detected in most of the selected food samples, however, sulfamonomethoxine was detected in meat. The determined level of sulfamonomethoxine were 0.03 and 0.06 mg/kg for beef that were below the MRLs.