• Korean Journal of Microbiology
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• v.20 no.4
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• pp.183-188
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• 1982

The neurotoxin of Clostridium botulinum type B was purified from a liquid culture. The purification steps consist of ammonium sulfate precipitation of whole culture, treatment of Polymin P(0.15%, v/v), gel filtration on Sephadex G-100 at pH5.6 and DEAE-Sephadex charomatography at pH8.0. The procedure recovered 17% of the toxin assayed in the starting culture. The toxin was homogeneous by sodium dodecyl sulfate(SDS)-polyacrylamide gel electrophoresis and had a molecular weight of 163, 000. Subunits of 106, 000 and 56, 000 molecular weight were found when purified toxin was treated with a disulfide-reducing agent and electro phoresed on SDS-polyacrylamide gels.

• Proceedings of the Korean Information Science Society Conference
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• 1999.10a
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• pp.228-230
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• 1999

공간 인덱스를 이용한 공간 질의 처리의 과정은 여과와 정제 단계로 이뤄진다. 여과 단계에서 후보 객체의 수를 줄이며, 정제 단계에서의 false-hit이 낮아지므로 불필요한 디스크 접근과 공간연산으로 인한 질의 처리 비용의 증대를 방지할 수 있다. 본 논문에서는 여과 단계에서 후보 객체를 최소화하기 위해 DTVF가 추가된 확장 R-tree를 제안한다. 제안된 기법에서는 n차원 상에 존재하는 공간 객체의 대표 정점들을 구석점 변환 기법을 이용하여 2n차원의 점으로 변환하고, 이 값을 확장된 R-tree라는 리프 노드의 DTVF에 유지한다. 공간 질의 처리시 여과 단계에서 DTVF를 이용하면 후보 객체 수를 최소화할 수 있으며, DTVF에 유지된 차원 변환된 값을 통해 후보 객체 선정에도 빠른 성능을 나타낸다. 제안된 기법은 공간 질의 처리시 여과 효율을 극대화하여 질의 처리 성능을 향상시킨다.

• Proceedings of the Korean Society of Marine Engineers Conference
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• 2006.06a
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• pp.145-146
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• 2006

The main objective of this research is to develop a system which will provide a more efficient fuel saying measure for the current marine products industry situation caused by the increased cost of oil. For that function, the developed system has been verified using the medium of blending oil known as the MF 30 class. As a result, MF 30 was confirmed meeting the International Standard for NOx emissions and content of Sulfur. Oil composition and soot level analysis showed that it is acceptable to use MF 30 class in condition of proper engine running operation and pre-refinery treatment.

• Korean Journal of Food Science and Technology
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• v.35 no.2
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• pp.260-265
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• 2003

Transglutaminase (TG) was prepared from Streptomyces mobaraensis to improve texture and self-life of food. In preliminary experiments, texture of the dough was not improved due to the interference in microbial TG reaction by proteases present in the crude enzyme. Among the cation exchange resins tested for the removal of proteases, MonoPlus S 100 was the most efficient. Further purification steps with a quaternary ammonia salt resin and gel permeation chromatography effectively removed proteases from crude enzyme. Molecular weight of purified enzyme was about 38,000 on SDS-polyacrylamide gel electrophoresis. Farinograph data showed the addition of purified enzyme to wheat flour gave higher stability and lower weakness values those that of crude enzyme.

• Journal of KIISE:Databases
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• v.27 no.4
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• pp.637-650
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• 2000

공간조인은 지리정보시스템에서 가장 중요한 연산 중의 하나이다. 본 논문에서는 공간조인 처리를 위하여 여과-정제 방법을 사용할 때 여과단계에서의 효과를 높이기 위하여 점 기반 approximation인 Representative Point (RP) 필터를 제안하였다. RP 필터는 실객체에서 선택된 몇 개의 점들로 구성된다. 이 점들을 객체의 근사치로 사용함으로써 공간조인의 여과단계에서 후보객체의 수를 줄이며, 따라서 공간조인의 수행 성능을 높일 수 있다. 또한 RP 필터의 여러 종류들을 제안하였으며 그 종류들의 성질과 성능을 비교분석 하였다. RP필터는 공간조인을 위한 기존의 approximation들에 비하여 생성 및 우지 비용이 매우 적으며 더 높은 필터율을 가진다. 또한 RP 필터는 기존의 approximation들과 달리 선객체에 대하여도 정의될 수 있기 때문에, 다각형 객체끼리의 공간조인 뿐만 아니라 다각형 객체와 선 객체간의 공간조인에도 적용할 수 있다.

• Proceedings of KOSOMES biannual meeting
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• 2006.11a
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• pp.291-297
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• 2006

Recently it is a tendency that the use of the heavy fuel oil is investigated even from the middle&small class vessel in order to reduce the operating cost of vessel caused by with rise of international gas price. In this study, analyzed the physical & chemical properties and examined the effect of refining treatment and a fuel oil additive for MF30 fuel oil which is a mixture fuel oil mixed M.G.O and the heavy oil MF380 use to be possible in the middle&small class vessel. As a results, the effects of two of pre-refinery treatment methods as centrifugal purifier and heating & homogenizing system(M.C.H) are some feeble, but the pour point and the flash point came to be low more or less. The effect of property improvement which is caused by with the fuel oil additive did not appear positively.

• Microbiology and Biotechnology Letters
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• v.19 no.3
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• pp.209-214
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• 1991

Purification of the bacteriocin from Lactococcus sp. 1112-1 was achieved by successive column chromatography on CM-Sephadex C-25 and Sephadex G-50, starting from cell disruption broth. 16.2% of the initial activity was recovered after this purification step and it was shown 123-fold increase in purification. Purified bacteriocin was shown a single band on SDS-polyacrylamide gel electrophoresis. This substance was rather stable at heat treatment and alkaline pH relatively. The residual antimicrobial activity was 38% when the bacteriocin was treated by heat at $100^{\circ}C$ for 60 min. And 23% of the activity remained at pH 8.0 after standing for 48 hr. The amino acid composition of purified bacteriocin was made up 26 residues.

• Journal of Life Science
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• v.13 no.5
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• pp.576-581
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• 2003

Recombinant Arabidopsis non-specific lipid transfer proteins (nsLTPs) was purified from yeast. In order to determine the effect of nsLTPs for an production of anthocyanin in perilla leaves, ‘Manchudlggae’ cultivar was grown at pots that had been applied with different concentration of nsLTPs. The anthocyanin content in AtnsLTP treated leaves increased above two-fold higher than that in control. Also chlorophyll content was increased 16%. It was presumed that AtnsLTPs could be applied to increase high quality of perilla leaves.

• Journal of Conservation Science
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• v.35 no.5
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• pp.453-469
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• 2019

The celadon jar with inscription of 'the Fourth Sunhwa Year' is an important chronology that shows the conditions of production of the early celadon due to the inscription on the bottom including its purpose, application, and the producer. This celadon jar has been restored several times in the past. However, concerns over the structural stability, such as the separation and level differences in the joined cracks, have risen because of the aging of the repair materials, which were subjected to various environmental changes over a long time. By examining the conditions of preservation, the major damage was identified as the '入' shaped crack on the front, the 'V' shaped restored part and the crack on its left side, and the 'J' shaped crack on the back side. In the past, the cracks were found to be joined using a refined lacquer containing camphor, drying oil, rosin, etc. mixed with soil powder. The joint line was repainted with the refined lacquer and covered with gold powder. The missing parts were restored with gypsum and colored with acrylic color. After that, the repair materials were aged and emergency treatment was performed at the National Museum of Korea in 1981. At that time, Cemedine C or Cemedine C mixed with microballoons was used for reinforcing the cracks. Conservation treatment focused on removing the past repair materials and reinforcing the physically fragile parts by joining and restoring them based on the examination of the preservation condition. in addition, the area around the restored part was colored for future exhibition.

• Microbiology and Biotechnology Letters
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• v.16 no.5
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• pp.335-342
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• 1988

An anion exchange chromatography was employed for the purification of mouse monoclonal antibodies from ascitic fluid and in vitro cultivation media. After cultivation of hybridomas, Alps 25-3, HCGK, A4W, and KW, producing IgG1, the culture supernatants were harvested by centrifugation, precipitated with 50-60％ ammonium sulfate, and dialyzed against 0.025 M Tris-HCI buffer (pH 8.2). Then the dialyzed samples were loaded into a DEAE-Trisacryl M anion exchange column. Monoclonal antibodies bound to the DEAE-Trisacryl M were eluted with 0.025 M Tris-HCI buffer (pH 8.2) containing 30-40 mM NaCl. In ammonium sulfate precipitation, the recovery of the monoclonal antibody was shown to be 90％ and 84％ from in vitro culture media containing 10％ and 2％ fetal bovine serum, respectively. On the other hand, the pretreatment by ultrafiltration enhanced the yield up to 91％ whereas the purity was lower than that by ammonium sulfate treatment. Subsequently, in the DEAE-Trisacryl M chromatographic separation, the purities and recoveries of all the monoclonal antibodies from both the in vitro culture supernatants and ascitic fluids were 70-80％ and 65％ respectively. The monoclonal antibody, Alps 25-3 could be further purified with a purity of 95％ through an immunoadsorbent chromatography.