• Korean Journal of Soil Science and Fertilizer
• /
• v.41 no.4
• /
• pp.267-272
• /
• 2008

Mid-infrared (MIR) spectroscopy, particularly Fourier transform infrared spectroscopy (FTIR), has emerged as an important analytical tool in quantification as well as identification of multi-atomic inorganic ions such as nitrate. In the present study, the possibility of quantifying soil nitrate via diffuse reflectance infrared Fourier transform spectroscopy (DRIFTS) without change of a sample phase or with least treated samples was examined. Four types of soils were spectrally characterized in terms of unique bands of soil contents and interferences with nitrate bands in the range of $2000-1000cm^{-1}$. In order to reduce the effects of soil composition on calibration model for nitrate, spectra transformed to the 1st order derivatives were used in the partial least squared regression (PLSR) model and the classification procedure associated with input soil types was involved in calibration system. PLSR calibration models for each soil type provided better performance results ($R^2$>0.95, RPD>6.0) than the model considering just one type of soil as a standard.

• Applied Biological Chemistry
• /
• v.35 no.1
• /
• pp.23-29
• /
• 1992

Bromelain was purified from Korean pineapple, Ananas comosus, L. The enzyme was purified about 21 fold by DEAF-cellulose ion-exchange chromatography and gel filtration on Sephadex G-150. Purified enzyme was confirmed as active single band by polyacrylamide electrophoresis and the molecular weight was estimated to be about 22,000 by SDS-PAGE. The optimum pH and temperature were 6.0 and $60^{\circ}C$, respectively. The range of its stability to the pH and temperature were respectively 5.0 to 7.0 and below $50^{\circ}C$. It was found that $Mn^{2+}$ increased the enzyme activity, whereas $Mg^{2+}\;and\;Fe^{2+}$ decreased it abruptly. The purified enzyme was inhibited by p-chloromercuribenzoic acid, indicating that reactive SH groups are required for the enzyme activity. The reaction of the enzyme followed typical Michaelis-Menten kinetics with Km value of $5.747{\times}10^{-4}\;M\;and\;Vmax\;of\;131.58\;{\mu}g/min$ for casein. When meat was treated with the enzyme, free soluble nitrogen and amino acid nitrogen increased as enzyme concentration increased.

• Journal of Conservation Science
• /
• v.26 no.4
• /
• pp.349-360
• /
• 2010

The Yeongsheon Sinwoldong three-storied stone pagoda (Treasure No. 465) composed mainly of drusy alkali-granite. The major rock-forming minerals are biotite, quartz, amphiboles, orthoclase and plagioclase. Yellowish brown and black discoloration are formed at the eight sculpture Buddha of the stylobate. A broken rock fragments in the roof material were repaired using epoxy resin and cement mortar in the past. As a result of the infrared thermography analysis from the pagoda, cracks and exfoliation were not serious. Also, P-XRF analysis showed that concentration of Fe (mean 5,599ppm) and S (mean 3,270ppm) were so high in yellowish discoloration parts. Black discoloration area was detected highly Mn (mean 2,155ppm) concentration around the eight sculpture Buddha of the stylobate. The main reason for these are inorganic contaminants from disengaged rock ingredient and organic contaminants from withered plant body. Degree of physical weathering is relatively high in the southern and northern side. The eastern and western side had similar with weathering condition. The northern and eastern side were serious discoloration and biological weathering relatively. Therefore, we suggest that the pagoda need to do cleaning of biological contaminant and conservation treatment to weakened materials of rock and long term monitoring.

• Food Science and Preservation
• /
• v.20 no.2
• /
• pp.227-233
• /
• 2013

This study was conducted to investigate the physicochemical characteristic of Carthamus tinctorius L. seed and to assess its total phenol content, total flavonoids content and antimicrobial activity. The moisture, crude protein, crude fat, crude ash and carbohydrates of the Carthamus tinctorius L. seed were 5.58, 37.16, 13.69, 3.52, and 40.05%, respectively. Total amino acid in Carthamus tinctorius L. seed was 391.99 mg%. The major free sugar of Carthamus tinctorius L. seed were fructose(3.29%) and sucrose(1.74%). Linoleic acid(79.46%) was a major fatty acids in the crude fat of Carthamus tinctorius L. seed. The K and Ca contents were the highest in Carthamus tinctorius L. seed. Total phenol and total flavonoids contents of the ethanolic extract were $55.52{\pm}0.99$ mg GAE/g and $78.69{\pm}0.91$ mg QE/g, respectively. The extract from Carthamus tinctorius L. seed showed growth inhibitory effect on Staphylococcus aureus, Salmonella typhimurium, Escheria coli, Candida albicans, Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, and Clustridium perfringens. These results indicate that the Carthamus tinctorius L. seed extract can inhibit food pathogen associated with total phenol and total flavonoids contents.

• The Journal of the Petrological Society of Korea
• /
• v.15 no.3 s.45
• /
• pp.128-138
• /
• 2006

The west stone pagoda of Gameunssji temple site (National Treasure No. 112) has been damaged mainly by fracture, exfoliation and granular disintegration. In this study, the source area of the rocks using the west stone pagoda was examined in terms of petrological feature, magnetic susceptibility, and ${\gamma}-ray$ spectrometer. The stones include abundant crystal fragments of biotite, quartz and feldspars in the fine-grained matrix; they are petrographically discriminated to vitric-crystal tuff or crystal tuff. Measured magnetic susceptibility values are of from 10 to 20 $({\times}10^{-3}\;SI\;unit)$. From the ${\gamma}-ray$ spectrometer measurement K, eU, and eTh contents of the stones are about 3%, 0 to 8ppm, and 9 to 18 ppm, respectively. These features are used as indicators to presume the source area of the stones. Comparing the petrographical and chemical characteristics between the stones of the west stone pagoda and the country rocks near the Gameunsaji temple site, it is suggested that the most similar country rock to the stones could be dacitic volcanic rocks of the Beomgokri group in the Waeup basin. The Beomgokri group is lithostratigraphically divided into Waeupri tuff, Yongdongri tuff and Beomgokri volcanic rocks. Among the three rocks, the crystal tuff of the Beomgokri volcanic rocks seems likely to have been the source rock of the stones of the west stone pagoda.

• The Journal of the Petrological Society of Korea
• /
• v.27 no.4
• /
• pp.207-222
• /
• 2018

Celestial Chart Stones (original and reproduction) in the National Palace Museum are representative scientific cultural heritage of Korea. Material analysis and nondestructive deterioration assessment were conducted for long-term preservation of these stones. Material analysis revealed that the original was composed of slate and the reproduction was made of dolostone. The original consists of quartz, mica, dolomite minerals, while the reproduction was made up of dolomite, calcite and forsterite. Major deterioration factors of the original stone were cracks and breakouts. In case of the reproduction, scratches and artificial materials were mainly observed. The green and black surface contaminants present at the sides and back of the two celestial chart stones were interpreted as resin-based paint materials. The physical property evaluation using ultrasonic velocity showed a low velocity in the upper left side of the original, while the front right side of the reproduction showed a weak property. Meanwhile, the To-Tc method using ultrasonic velocity was applied to major cracks that impede stability of the original. As a result, it has been calculated that the beginning and the center of the crack are the deepest.

• Korean Journal of Heritage: History & Science
• /
• v.45 no.1
• /
• pp.70-85
• /
• 2012

Uiseong Tamni-ri Five-storied Stone Pagoda is composed of andesitic tuff and partially combined with tuff breccia and fine-grained granite. The andesitic tuff is identical to basement rock of Geumseongsan Mountain based on lithological, mineralogical and geochemical characteristics. The pagoda has suffered physical weathering such as crack and scaling, discoloration and biological colonization with complex reaction. Expecially, dark gray and brown discoloration appeared whole over the surface of the pagoda, and three to five-layered exfoliation and granular disintegration dominantly occurred in the fourth and fifth roof stones. It is assuming that the stone elements of the pagoda are evaluated as third to forth grades (average third grade) of weathering compared to fresh rock in Geumseongsan Mountain. The physical strength of the stone elements shows low values in the south and west sides of the pagoda that corresponds high weathering degree of the west side due to exfoliation, crack and granular disintegration. It is necessary to investigate the pagoda for precise deterioration assessment, monitoring and conservation treatment.

• Korean journal of applied entomology
• /
• v.61 no.1
• /
• pp.249-254
• /
• 2022

Silkworms, which have for long been used as an insect resource for industrialization, have recently attracted attention as potential bio-factories for the production of novel biomaterials. In this regard, material production is typically achieved based on transformation technology, mediated via microinjection, in which a target gene is inserted into eggs containing an embryo. However, an essential step in the microinjection procedure is egg fixation, which can be a time-consuming and laborious task. Therefore, in this study, using the 3DCADian program, we adopted a 3D printing approach to model egg liners and glue drawers, which can contribute to facilitating egg alignment and fixation, thereby enhancing transformation efficiency by reducing time consumption and fatigue. After rendering using Fusion 360, the two supplementary tools were produced by printing with nylon resin (PA12) and Sinterit Lisa Pro. Subsequent analysis of the time required to fix eggs on glass slides using the two manufactured tools, revealed that the processing time was reduced by approximately 18.6% when the two tools were used compared with when these tools were not used. These innovations not only reduced fatigue but also contributed to more effective use of the microscope and manipulator for microinjection. Consequently, we believe that with additional research and refinement, the egg liner and glue drawer developed in this study could be used to enhance silkworm transformation efficiency and study similar transformation systems in other industrial insects.

• Korean Journal of Weed Science
• /
• v.11 no.3
• /
• pp.195-204
• /
• 1991

This study was conducted to determine physiological responses rice cultivars to glufosinate-ammonium. Changes of total protein content, protein population, glutamine synthetase activity, accumulated ammonia content and free amino acid composition were examined in both tolerant and susceptible rice cultivars. Tamjinbyeo and Fukei 126 showed relatively tolerant response to glufosinate-ammonium while Namyeongbyeo, Palgongbyeo and Youngdugbyeo were susceptible to it. Total protein content of two tolerant cultivars was 93.2 of the untreated control in average but three susceptible cultivars showed 76.5 of the untreated control in average, showing 16.7 difference. Protein profiles of the tolerant cultivars seemed to be not affected by glufosinate-ammonium treatment. However, in susceptible cultivar like Namyeongbyeo, 10ppm of glufosinate-ammonium application resulted in decrease of band density or disapperance of spot density in near 20kD and in between 45kD and 66kD on 2D-PAGE. Glutamine synthetase activity in susceptible cultivars was markedly inhibited by glufosinate-ammonium treatment, accompanying remarkable increase of ammonia content by three times greater than tolerant cultivars, and markedly increase of glutamic acid, showing 430% of the untreated control.

• Korean Journal of Agricultural Science
• /
• v.20 no.2
• /
• pp.109-116
• /
• 1993

This experiment was carried out to investigate the competition in mono-and binary-associations of four forage species; ochardgrass (Dactylis glomerata L. cv. Potomac), tallfescue (Festuca arundinacea Scnreb, cv. Fawn), white clover (Trifolium repns L. cv. California), and red clover (Trifolium pratense L. cv. Kenland) as affected by different sowing rate. Each species was grown in interspecific stands in field. And samplings of shoot were taken at first cliping stages. The interspecific competition was evaluated by calculating traditional methods: aggressivity, relative yield total (RYT), relative crowding coefficient (RCC), and compensation index (CI) with shoot dry matter production and calories of nutrient ingredient of each species in mono-and binary-mixture. Compared mixture with monoculture. RYT based on dry weight was lucrative in mixture except for orchardgrass-redcover (1:0.1,1:1). RYT of calculated calories with produced nutrients was in inlucrative in mixture of orchardgrass-redclover's 1:0.1 mixture by RYT<1.0. Generally, RYT was the highest in mixture of 1:0.5 sowing rate. Aggressivity of grass was poor competition tendency that raised up legume's sowing rate, particularly, with whiteclover. In grasses, tallfescue was stronger competitor than orchardgrass with legume in mixture, RCC of grasses against legume's growth was depressed in higher sowing rate with legume. Grass's growth depression was severer with whiteclover than redclover in mixture.