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Analysis of Food Components of Carthamus Tinctorius L. Seed and its Antimicrobial Activity

홍화씨의 식품학적 성분 분석 및 항균활성

  • Yu, Seok-Yeong (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Lee, Young-Jun (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Kang, Suk-Nam (Department of Animal Resources Technology, Gyeongnam National University of Science and Technology) ;
  • Lee, Seong-Kap (Department of Food and Biotechnology, Hoseo University) ;
  • Jang, Jung-Young (Department of Food Science, Kongju National University) ;
  • Lee, Hyo-Ku (Department of Food Science, Kongju National University) ;
  • Lim, Jeong-Ho (Korea Food Research Institute) ;
  • Lee, Ok-Hwan (Department of Food Science and Biotechnology, Kangwon National University)
  • 유석영 (강원대학교 식품생명공학과) ;
  • 이영준 (강원대학교 식품생명공학과) ;
  • 강석남 (경남과학기술대학교 동물소재공학과) ;
  • 이성갑 (호서대학교 식품생명공학과) ;
  • 장중영 (공주대학교 식품공학과) ;
  • 이효구 (공주대학교 식품공학과) ;
  • 임정호 (한국식품연구원) ;
  • 이옥환 (강원대학교 식품생명공학과)
  • Received : 2012.11.28
  • Accepted : 2013.04.04
  • Published : 2013.04.30

Abstract

This study was conducted to investigate the physicochemical characteristic of Carthamus tinctorius L. seed and to assess its total phenol content, total flavonoids content and antimicrobial activity. The moisture, crude protein, crude fat, crude ash and carbohydrates of the Carthamus tinctorius L. seed were 5.58, 37.16, 13.69, 3.52, and 40.05%, respectively. Total amino acid in Carthamus tinctorius L. seed was 391.99 mg%. The major free sugar of Carthamus tinctorius L. seed were fructose(3.29%) and sucrose(1.74%). Linoleic acid(79.46%) was a major fatty acids in the crude fat of Carthamus tinctorius L. seed. The K and Ca contents were the highest in Carthamus tinctorius L. seed. Total phenol and total flavonoids contents of the ethanolic extract were $55.52{\pm}0.99$ mg GAE/g and $78.69{\pm}0.91$ mg QE/g, respectively. The extract from Carthamus tinctorius L. seed showed growth inhibitory effect on Staphylococcus aureus, Salmonella typhimurium, Escheria coli, Candida albicans, Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus, and Clustridium perfringens. These results indicate that the Carthamus tinctorius L. seed extract can inhibit food pathogen associated with total phenol and total flavonoids contents.

본 연구는 홍화씨의 식품학적 성분 및 항균활성을 분석하였다. 홍화씨의 수분함량은 $5.58{\pm}0.09%$, 조단백질 $37.16{\pm}0.85%$, 조회분 $3.52{\pm}0.04%$, 조지방 $13.69{\pm}8.98%$, 탄수화물 40.05%이었다. 홍화씨의 총 아미노산은 391.99 mg%이고, 이중 필수아미노산의 함량은 122.97 mg%이었다. 홍화씨의 아미노산 중 alanine (75.43 mg%), arginine (37.93 mg%) 및 aspartic acid (33.24mg%)을 많이 함유하고 있었다. 홍화씨의 주요 유리당은 fructose가 3.29%이었고, linoleic acid (C18:2)는 약 79.46%의 비율을 차지하여 홍화씨의 조지방 중 가장 많은 것으로 나타났다. 또한, Oleic acid (C18:1)도 약 10.42%를 함유하여 불포화지방산의 함유량도 매우 높게 나타났다. 홍화씨의 무기질 함량 측정 결과, K 및 Ca의 함량이 1,528 및 1,005 mg%으로 가장 많이 함유되어 있었다. 총 페놀 및 총 플라보노이드 함량은 $55.52{\pm}0.99$ mg GAE/g, $78.69{\pm}0.91$ mg QE/g으로 나타났다. 홍화씨의 항균활성은 농도가 증가함에 따라 Staphylococcus aureus, Salmonella typhimurium, Escheria coli, Candida albicans, Bacillus cereus, Listeria monocytogenes, Vibrio parahaemolyticus 및 Clustridium perfringens 균에 대한 항균 효과가 높아지는 것으로 나타났다.

Keywords

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