• Title/Summary/Keyword: 생활력

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In Vitro Assessment on Viability of Human Periodontal Ligament Cells after Storage in Chlorophyllin-added Medium (클로로필린이 첨가된 탈구치아 보존액에서의 치주인대 세포 생활력에 대한 연구)

  • Chung, Won-Gyun;Kim, Jin;Lee, Eun-Ju;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
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    • v.27 no.6
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    • pp.600-611
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    • 2002
  • 본 연구의 목적은 클로로필린이 치주인대 세포 생활력에 미치는 영향을 평가하는데 있다. 치주인대 세포는 건강한 사람에서 발치한 소구치의 치주인대 조직으로부터 채취하여 배양하였다. 비교 기준을 설정하기 위하여, HBSS 내에서 $37^{\ circ}C$로 보관한 치주인대 세포수의 50%가 생존하는데 소요되는 시간을 MTT 분석법에 의해 측정하였다. 그 결과는 6시간이었다. HBSS에 클로로필린 10, 100, 500 nM이 각각 첨가된 3군의 실험 보존액과 F-medium, ViaSpan, Likorol 등 모두 6군의 실험 보존액을 96-well plate에 접종한 후, 치주인대 세포를 동일한 수로 분주하였다. 이를 6시간동안 보관한 후, 각 실험군 보존액에서의 세포 생활력을 MTT 분석법으로 측정하였다 또한, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS군의 세포들에 대해 유식세포 계측을 시행하여 각각의 세포주기를 분석하였다. 실험 결과, 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포들이 가장 높은 생활력을 나타내었으며, 클로로필린이 첨가되지 않은 HBSS에 비해서 유의하게 양호한 세포 생활력 유지 능력을 보였다. 클로로필린으로 처치한 세포들은 클로로필린의 농도가 커짐에 따라 세포 생활력이 증가하는 양상을 나타내었다. 유식세포 계측 결과, HBSS와 F-medium 및 클로로필린 500 nM이 첨가된 HBSS에서 보관한 세포의 77~80%가 G0-G1 단계의 세포 주기로 측정되어, 대부분의 세포들이 안정된 세포 대사 상태를 보이는 것으로 나타났다. 결론적으로, 클로로필린 처치는 치주인대 세포의 생활력 유지에 도움이 되는 것으로 사료된다.

인산가용화 사상균 Aspergillus sp. PS-104의 생활력 유지에 미치는 유기물 첨가 효과

  • Tae, Eon-Hui;Choe, Ung-Gyu;Lee, Tae-Geun;Gang, Seon-Cheol
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.439-440
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    • 2000
  • This study was conducted to preserve viability of Aspergillus sp. PS-104, a phosphate-solubilizing fungus, for more than 6 months. The number of Aspergillus sp. PS-104 conidia stored at $25^{\circ}C$ with various concentrations of additives was counted on potato dextrose agar at the intervals of 2 weeks. When PEG 200 was added with concentration of 0.1%, viability of the conidia was increased more than 10 times to compare with control.

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PULP VITALITY TEST OF TEETH AFTER TRAUMATIC INJURIES USING LASER DOPPLER FLOWMETRY - A CASE REPORT (외상 받은 치아의 치수생활력 검사 - Laser Doppler flowmetry를 이용한 증례보고)

  • Song, Yun-Ju;Kim, Hyun-Jung;Nam, Soon-Hyeun;Kim, Young-Jin
    • Journal of the korean academy of Pediatric Dentistry
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    • v.26 no.2
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    • pp.310-316
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    • 1999
  • In the traumatized teeth, the major complications are pulp necrosis, and root resorption. The factors influencing to prognosis are the state of root development, type of injuries, quality of fixation. There are good prognosis in the case of immature teeth, minor luxation injuries. To minimize of occurrence of these complication, it is very important to determine the pulp vitality. This can help us decide whether or not to treat the teeth endodontically. But, it is difficult to determine of pulp vitality in immature teeth or traumatized teeth. In this case, pulp vitality test was done periodically on the traumatized upper central incisors, and the results were different among cold test, electric pulp test, and laser Doppler flowmetry. The conclusions were obtained from this case can be summarized as follows; 1. Pulp vitality of traumatized teeth should be negative early, but with time going, pulp vitality could be recovered. 2. The positive response of pulp vitality test was detected earlier in laser Doppler flowmetry. 3. Between cold test and electric pulp test, cold test was more reliable in determining pulp vitality.

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Influencing Factors on College Adjustment of Nursing Students : The Meaning of Life, Empathy Skills, Resilience (간호대학생의 대학생활 적응 영향요인 : 생의 의미, 공감능력, 극복력을 중심으로)

  • Lee, Jong-Lan;Park, Sun-Jung
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.20 no.11
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    • pp.66-75
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    • 2019
  • In an attempt to provide basic information for the development of programs geared toward facilitating college adjustment of nursing students, this study was undertaken to verify the meaning of life, empathy skills and resilience, as factors affecting the adjustment of nursing students to college and factors influencing relationships of the variables. Between March 1 to April 30, 2019, 531 nursing students in two different colleges located in Gyeonggi Province were enrolled to undertake a survey. The data were analyzed by descriptive statistics, Pearson's correlation coefficient, t-tests, ANOVA, and stepwise multiple regression. Results reveal that the meaning of life had a significant positive correlation with empathy skills, resilience and the degree of college adjustment. It was also ascertained that college adjustment was better when the meaning of life, empathy skills and resilience were greater. Factors affecting college adjustment include gender, grade, education at admission, health status, major satisfaction, stress, family economy, meaning of life and resilience; these variables had a 44.8% explain ability of college adjustment. Considering the findings of this study, we propose that sustained education and program development are required to enhance the meaning of life, empathy skills and resilience of college students to ensure their successful college adjustment.

The Relationship Between the Married Middle-aged Women's Life Stress and Depression -The Mediating Effects of Distress Tolerance and Rumination- (중년 기혼 여성의 생활스트레스와 우울의 관계 -고통감내력과 반추의 매개효과-)

  • Kim, Eun-Suk;Chung, Eun-Jung
    • The Journal of the Korea Contents Association
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    • v.19 no.4
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    • pp.638-650
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    • 2019
  • This study collected 328 self-reporting questionnaires from the married middle-aged women residing in Seoul, Gyeonggi and Daejeon province to explore the mediating effects between distress tolerance and rumination in the relations of life stress and depression. SPSS 20.0 and AMOS 20.0 were used to analyze descriptive statistics, correlation, and structural equation. Following is the summarizaton of the study. First, the life stress showed a significant positive correlation with rumination and depression, and meaningful negative correlation wiith distress tolerance. Also, it showed negative correlation with distress tolerance and rumination. Second, each distress tolerance and rumination showed meaningful mediating effects in the relations of life stress and depression. Third, dual mediating effect of distress tolerance and rumination is showed meaningful in the relations of life stress and depression. Based on the results above, we verified that three variables, life stress, distress tolerance and rumination, affect depression significantly in married middle-aged women and discussed the significance of this result, limitations and suggestions for future studies.

The Influence on College Life Adjustment of Separation Individuation and Family Cohesion (분리 개별화와 가족 응집력이 대학 생활 적응에 미치는 영향)

  • 이희자
    • Journal of Families and Better Life
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    • v.12 no.2
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    • pp.187-194
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    • 1994
  • 본 연구는 대학생들의 부모로부터의 심리적 분리개별화와 가족응집력이 그들의 대학생활적응에 미치는 영향을 조사하여, 대학생활 적응에 영향을 주는것이 부모로부터의 독립인지 가족의 친밀감과 지지인지 알아보고, 두 요인이 모두 영향을 준다면 각각 대학생활의 어떤 영역에 영향을 주는지 알아보고자 하였다. 중다회귀분석을 사용하여, 다음과 같은 연구결과 를 얻었다. 첫째, 부모로부터의 심리적 독립에서 갈등적 독립이 높을수록, 태도적 독립이 낮을수록 학업문제의 적응은 높았다. 갈등적 독립이 높을수록 심리적 문제와 진로, 취업문제의 적응은 낮았다. 태도적 독립과 기능적 독립이 높을수록 교수와의 관계에 있어서 적응은 낮게 나타났다. 기능적 독립이 발달한 학생은 교우와의 관계에서 높은 적응을 나타났다. 정서적 독립이 높은 학생은 전반적인 적응이 높게 나타났다. 둘째, 가족응집력은 대학생활에서 진로, 취업문제와 교유관계에 있어서 적응을 설명하고 있다. 그러나 남학생에게 가족응집력은 심리적 문제의 적응에 부적인 영향을 나타내며, 여학생에게는 가족응집력이 높을수록 교우관계와 진로, 취업문제의 적응이 높게 나타났다.

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Biologic Response of Human Deciduous Dental Pulp Cells on Newly Developed MTA-like Materials (새로 개발된 MTA 유사 재료에 대한 유치 치수세포의 생물학적 반응)

  • Lee, Haewon;Shin, Yooseok;Jung, Jaeeun;Kim, Seongoh;Lee, Jaeho;Song, Jeseon
    • Journal of the korean academy of Pediatric Dentistry
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    • v.42 no.4
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    • pp.291-301
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    • 2015
  • This study compared the in vitro cell viability and differentiation potentials of human deciduous dental pulp cells (DPCs) on mineral trioxide aggregate (MTA)-like products (ProRoot MTA, RetroMTA and Endocem Zr). The experimental materials were prepared as circular discs, which were used to test the effects of the materials on the viability of human DPCs when placed in direct and indirect contact. Furthermore, the pH of the extracted materials was recorded, and their effect on cell differentiation potential was evaluated by evaluating the alkaline phosphatase (ALP) activity and Alizarin Red S staining of DPCs incubated with the test materials. In direct contact, the cell viability of human DPCs was higher with ProRoot MTA and RetroMTA than with Endocem Zr. However, when in indirect contact, the cell viability of human DPCs was generally higher in Endocem Zr than in ProRoot MTA and Retro MTA. With respect to pH, the alkalinity was lower for Endocem Zr than for the other test materials. The ALP activities of the cells were not enhanced by any of the experimental materials. Alizarin Red S staining of the tested human DPCs revealed that their differentiation potential was lower than for cells incubated with osteogenic induction medium. While there were differences in the responses of the human DPCs to the test materials, all displayed degrees of cytotoxicity and were unable to enhance either the viability or differentiation of human DPCs. However, Endocem Zr exhibited better cell viability and was less alkaline than the other test materials.

The Effect of Self-Control on Life Satisfaction of Youth Participating in Drone Program (드론프로그램 참여활동 청소년의 자기통제력이 생활만족도에 미치는 영향)

  • Kim kwang youl;Kim Youn soo
    • The Journal of the Convergence on Culture Technology
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    • v.9 no.6
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    • pp.9-15
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    • 2023
  • This study investigated the effect of self-control on life satisfaction of youth participating in drone programs. Youth participating in drone program activities in the metropolitan area (Seoul, Gyeonggi) were conducted as questionnaires. The survey data were coded and statistically analyzed. The statistical significance level was ⍺= .05. The results of the study are as follows. First, simple task preferences and physical activity preferences affect mental health. Second, the pursuit of adventure affects happiness. Third, pursuit of adventure and self-centered factors affect autonomy and self-esteem. In conclusion, it can be judged that adolescents are partially satisfied with their lives through self-control.

University Students' Life Skill Comparison in Japan and Korea (일본과 한국 대학생의 생활력에 관한 국제비교)

  • Ueno, Akiko;Kim, Sung-Gyo;Yoshihara, Takae;Ogawa, Hiroko;Muro, Masako
    • Journal of Korean Home Economics Education Association
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    • v.22 no.4
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    • pp.31-49
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    • 2010
  • This study held two objectives. One was to clarify life skill differences between Japanese and Korean university students. The other was to gain suggestions for further development of Japanese and Korean home economics education. A survey on life skills was delivered to university students in Japan and Korea in 2008. The survey included 82 life skills. The participants were asked the same three questions for each life skill: if the life skill was one a participant was already practicing ("Practice"), if the life skill was one the participant wanted to learn more to improve his/her life ("To be improved"), and if the life skill was one the participant expected people should learn in Home Economics ("Expectation"). The results did not show outstanding differences with the "Practice" rates between Japan and Korea, but characterized strength and weakness of each country's life skill "Practice." Both Japanese and Korean participants mainly showed a greater desire to improve the life skills they indicated low "Practice" for. As well, the Korean participants revealed significantly lower "Expectation" to learn most of the life skills. As a result, this study created several implications for Japanese and Korean home economics education. For example, one implication is necessity to increase substantively enough class hours for all school levels. Another is promoting the social understanding for the meaningfulness of home economics education.

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The Relationship between Life Stress and Resilience among Dental Hygiene Students (일부 치위생과 학생들의 생활스트레스와 극복력의 관계)

  • Yoon, Mi-Suk;Jung, Hyo-Jung
    • Journal of dental hygiene science
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    • v.16 no.5
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    • pp.384-392
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    • 2016
  • The purpose of this study was to investigate life stress and resilience levels, and the relationship between life stress and resilience among dental hygiene students. From July 11, 2016 to July 29, 2016, questionnaires were used to survey dental hygiene students in several areas, after which the data collected from 274 questionnaires were analyzed with IBM SPSS Statistics ver. 21.0 for Windows. The results showed a significant negative correlation between life stress and resilience for dental hygiene students in all factors (p<0.05, p<0.001). This means that higher the resilience, the lower the life stress. Therefore, it is necessary to enhance resilience as a preventive dimension and develop associated program to help dental hygiene students cope with life stress.