• Tuberculosis and Respiratory Diseases
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• v.51 no.6
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• pp.559-569
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• 2001

Background : Pleural effusion is one of the most common clinical manifestations associated with a variety of pulmonary diseases such as malignancy, tuberculosis, and pneumonia. However, there are no useful laboratory tests to determine the specific cause of pleural effusion. Therefore, an attempt was made to analyze the various types of pleural effusion and search for useful laboratory tests for pleural effusion in order to differentiate between the diseases, especially between a malignant pleural effusion and a non-malignant pleural effusion. Methods : 93 patients with a pleural effusion, who visited the Severance hospital from January 1998 to August 1999, were enrolled in this study. Ultrasound-guided thoracentesis was done and a confirmational diagnosis was made by a gram stain, bacterial culture, Ziehl-Neelsen stain, a mycobacterial culture, a pleural biopsy and cytology. Results : The male to female ratio was 56 : 37 and the average age was $47.1{\pm}21.8$ years. There were 16 cases with a malignant effusion, 12 cases with a para-malignant effusion, 36 cases with tuberculosis, 22 cases with a para-pneumonic effusion, and 7 cases with transudate. The LDH2 fraction was significantly higher in the para-malignant effusion group compared to the para-pneumonic effusion group [$30.6{\pm}6.4%$ and $20.2{\pm}7.5%$, respectively (p<0.05)] and both the LDH1 and LDH2 fraction was significantly in the para-malignant effusion group compared to those with tuberculosis [$16.4{\pm}7.2%$ vs. $7.6{\pm}4.7%$, and $30.6{\pm}6.4%$ vs.$17.6{\pm}6.3%$, respectively (p<0.05)]. The pleural effusion/serum LDH4 fraction ratio was significantly lower in the malignant effusion group compared to those with tuberculosis [$1.5{\pm}0.8$ vs. $2.1{\pm}0.6$, respectively (p<0.05)]. The LDH4 fraction and the pleural effusion/serum LDH4 fraction ratio was significantly lower in the para-malignant effusion group compared to those with tuberculosis [$17.0{\pm}5.8%$ vs. $23.5{\pm}4.6%$ and $1.3{\pm}0.4$ vs. $2.1{\pm}0.6$, respectively (p<0.05)]. Conclusion : These results suggest that the LDH isoenzyme was the only useful biochemical test for a differential diagnosis of the various diseases. In particular, the most useful test was the pleural effusion/serum LDH4 fraction ratio to distinguish between a para-malignant effusion and a tuberculous effusion.

• Korean Journal of Food Science and Technology
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• v.48 no.5
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• pp.437-444
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• 2016

Rapid and reliable identification of microorganisms is important to maintain food quality and to control safety. MALDI-TOF MS-based identification methods are relatively fast and simple compared to other conventional methods including gram staining and biochemical characterization. A colony on subcultured media can be directly prepared on the analysis plate without further complex treatments. In this study, we confirmed the applicability of MALDI-TOF MS-based identification of foodborne pathogens such as Salmonella Enteritidis/Typhimurium, Staphylococcus aureus, Vibrio parahaemolyticus, Clostridium perfringens, Listeria monocytogenes, Yersinia enterocolitica, Bacillus cereus, Campylobacter jejuni, Campylobacter coli, and Cronobacter sakazakii on the Korea Food Standard Codex. MALDI-TOF MS data of the pathogenic reference strains were incorporated into a commercial MicroID (ASTA Inc.) database. Other pathogenic reference strains and seven isolates from various food samples were correctly identified to the species level by using the MicroID database. In conclusion, MALDI-TOF MS is comparable with commercial biochemical identification.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2003.09a
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• pp.48-48
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• 2003

Abstract: 현대 질병의 최종진단은 세포수준의 해부형태학적 연구가 일반적이며 광학현미경이나 전자현미경을 이용한 병리조직학적 소견을 바탕으로 진단되어지고 있다. 이러한 진단에는 In vivo 검사가 거의 불가능하여 주로 생검을 통한 연구만 수행되고 있어 진단함에 있어 단계적인 불편이 초래될 뿐만 아니라 악성 종양의 전이를 유발시킬 수 있고 또한 생리 및 생화학적 분석이 어렵다. 이러한 문제점을 해결하기 위해 고분해능의 RF Surface Coil을 개발하여 In vivo 및 비침습적인 방법인 MR Technology를 이용하고자 한다. Introduction: 피부조직과 같은 미세 인체구조 연구를 위해 고해상도 3T MRI 시스템에 적합한 고분해능의 RF surface coil을 개발하고 있다. In vivo 연구를 위한 여러 parameter를 최적화하여 기능영상에도 부합된다. 비침습적인 In vivo 검사에 의한 세포수준의 극 미세구조의 연구가 가능해짐으로써 과거 시행하던 침습적인 생검없이 각종질환의 진단적 접근이 병리학적 수준으로 향상되어 질병의 정확한 진단이 가능해지게 될 것이다. Method: 고분해능의 RF Surface Coil을 제작하여 3T MR 장비에서 피부 미세구조연구에 보다 적합하도록 In vivo 및 In vitro 실험을 수행하였다. In vitro 실험은 In vivo 연구를 위한 여러 parameter들을 최적화하기 위한 기초 실험을 하였고 다양한 팬톰들을 이용하여 Tl 강조영상, T2 강조영상을 획득하였으며, SNR을 높이기 위한 개선에 대한 연구를 수행하였다. In vivo 실험은 정상피부에서 다양한 부위에 대한 피부영상의 예비 연구를 수행하였다. Result and Discussion: 비침습적인 In vivo 검사에 의한 세포수준의 극 미세구조의 연구가 가능해짐으로써 과거 시행하던 침습적인 생검없이 각종질환의 진단적 접근이 병리학적 수준에서 가능해짐으로써 질병의 정확한 진단이 가능해지게 될 것이다. Acknowledgement: 본 연구는 2002 년도 한국과학재단 목적기초연구사업 (과제번호 : R0l-2002-000-00294-0 (2002)) 지원아래 수행되었다.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.221-228
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• 2020

Ochrobactrum anthropi is a non-fermentative oxidative gram-negative bacillus that produces oxidase. Distinguishing a mixed culture with non-fermenting bacteria having a similar appearance and oxidase-positive is difficult, and there is a limit to accurate identification with a biochemical identification system. This paper proposes that the Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Platform (MALDI-TOF) method is useful for classifying bacteria that are difficult to identify using biochemical testing methods. As a result of analyzing five cases of O. anthropi examined using MicroScan, it took 6.5 days to the final report, which was 3.5 days more than the 3.0 days of E. coli. The pus sample in patient 5 was a mixed infection with Achromobacter xylosoxidans, and it took 11.3 days because of multiple subculture and retests. Four patients were over 60 years old with an underlying disease, and the possibility of opportunistic and nosocomial infections could not be excluded. Among them, samples collected after 92 days of hospitalization were resistant to imipenem and meropenem. Therefore, an examination using the MALDI-TOF method will be useful for the rapid and adequate treatment of patients with difficult identification, such as O. anthropi.

• Korean Journal of Microbiology
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• v.36 no.3
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• pp.242-248
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• 2000

Since the official allowance of bottled water at Korean domestic market in 1995, Pseudomo~zas aemginosu has been detected from 2.3% and 1.2% of source and products of bottled water sa~nple tested, respectively. according to the nation-wide dala froin May 1995 to December 1996. Therefore, P aeivginosa was the second most important parameter, next to colifoi~ns, anlong the bacieriological parameters regulated for bonled water. The official standard method initially adopted the Japanese officlal method and Standard Methods of the US, w~hich is using aspai-agiii-acetamid mnedia(A-A method). how eve^; the method showed low specificity regardless of the high sensitivity. The $42^{\circ}C$ growth test was the best biochemical featu1-e differentiating the P uelarginosu 6-om P aei-uginosa-like species such as P puririir and P Jhoi.escens amo1zg the other characierisiics such as fluorescence pigment_ pyocyanin, casein hy&olysis, etc. Thel-efore, addition of the $42^{\circ}C$ growth Lest in advance ofthe biochemical identification test, when sainple is positive by A-A method, should strengthen the spec~IiclQ w~tli ~ninin~um addition of testing load.

• The Korean Journal of Cytopathology
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• v.7 no.1
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• pp.1-11
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• 1996

From the concepts of cellular pathology and of exfoliative cytology, as elucidated by Virchow and Papanicolaou respectively in the late 19th and early 20th century, have evolved the primary methods for the diagnosis of cancer today. From Papanicolaou's concept of exfoliative cytology developed fine needle aspiration biopsy in the early 1960's, this has become a major diagnostic procedure and has contributed to a significant reduction in open biopsies and, therefore, to medical cost-effectiveness immunobiochemical techniques provided us with a supplement to cancer diagnosis in the 1980's. The immunoperoxidase method, using monoclonal antibodies, is applied primarily as an ancillary measure to elucidate the nature of cancers The availability of specific monoclonal antibodies has greatly facilitated the identification of cell products or surface markers. For example, antibodies directed against intermediate filaments have proved to be of value in determining the histogenesis oi poorly differentiated neoplasms. Tumor markers may serve as biochemical indicators of the presence of a neoplasm. They can be detected In plasma and other body fluids. Their concentration can be applied as a diagnostic test, for monitoring the clinical course of known cancer, and as a screening measure to detect certain cancers in a population at risk. Flow cytometry is a useful tool for distinguishing several cell characteristics, such as the immunophenotype of leukemia-lymphoma cells, the DNA content of neoplastic cells, and cell proliferation rate. Molecular biologic techniques provided a giant step for the management of cancer patients encompassing diagnosis, prognostic evaluation, and therapy. Nucleic acid hybridization techniques are utilized as Southern, Northern, and dot blots and in situ hybridization. Molecular biology and its techniques may bring a blight new horizon for understanding cancer biology and in designing therapy on the basis of gene manipulation.

• The Journal of the Korea Contents Association
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• v.10 no.7
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• pp.259-267
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• 2010

To evaluate an effect of liver damage on ethanol and xylene exposure, experiments on normal male rats of the S-D strain were performed in 4 groups. The biochemical results suggest that the ethanol group had significantly higher levels of AST, ALT, LDH and also, the xylene group had notably higher levels of AST, ALT, LDH along with MMHPA than those of the control groups. The levels of AST, ALT and LDH in the ethanol+xylene group were drastically higher than those in the control, ethanol and xylene groups. But, there were significantly lower ALP levels in the xylene and ethanol+xylene groups than both the control and ethanol groups. The histological features of rat livers treated with alcohol, or xylene proved to be normal. But the rat livers treated with ethanol+xylene showed mild to moderate necrosis and inflammation as well as minimal fatty changes. The results in this experiment suggest that liver functions decreased when medicated together with xylene and ethanol rather than solely with xylene.

• Journal of agricultural medicine and community health
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• v.43 no.2
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• pp.108-113
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• 2018

목적 글루포시네이트암모늄은 비선택성 제초제로 1984년 일본에서 개발되었으며, 급성 중독시에는 위장관계 증상뿐만 아니라 의식변화, 경련, 호흡부전 및 기억소실 등과 같은 다양한 임상증상을 보이는 것으로 알려져 있다. 한국에는 1994년 소개된 이후로 널리 사용되고 있으며, 사용량의 증가와함께 음독환자가 점점 증가하여 사회적 문제가 되고 있다. 저자들은 최근 글로포시네이트 중독 환자에서 초기치료 후 지연성 악화를 보였던 2례를 문헌고찰과 함께 보고하고자 한다. 방법 및 결과 첫 번째 증례는 62세의 남자환자로 음주상태에서 글루포시네이트암모늄 음독 후 의식 저하 상태로 발견되어 응급실로 이송되었다. 응급 위세척을 시행 후 보존적 치료 및 집중모니터링을 유지하였고, 의식수준은 6시간 후에 거의 명료한 상태로 호전되었다. 지연성 악화의 가능성에 대해설명하고 지속적인 경과관찰 및 치료를 위해 집중치료실 입원이 필요함에 대해 설명하였으나, 환자와 보호자는 타상급병원으로의 전원을 원하였다. 환자는 이송 중에 발생한 심폐정지로 심폐소생술을 시행하였으나, 회복되지 못하고 사망하였다. 두 번째 증례는 54세의 남자 환자로 글루포시네이트암모늄 음독 후 발생한 의식 변화로 응급실로 이송되었다. 내원시 의식은 혼미한 상태였으며 앞의 증례와 같이 응급 위세척을 시행하였다. 본 환자는 초기치료 후 본원 집중치료실에서 지속적인 혈액 및 생화학적 검사를 시행하였으며, 집중모니터링 및 치료를 유지하였다. 입원 2일째 혈액검사 소견은 호전 중이었으나, 경련이 발생하였으며 의식은 혼미한 상태가 지속되었다. 신경과 협진하여 뇌파검사를 시행 후 항경련제를 증량한 후로 경련은 발생하지 않았다. 입원 7일째에는 의식 수준은 거의 명료한 상태까지 호전되었다. 입원 13일째 혈액 및 생화학검사에서 약간의 혈색소 감소 외에는 정상소견을 보였으며, 뚜렷한 신경학적 합병증 없이 퇴원하였다. 결론 급성 글루포시네이트중독 환자는 매우 다양한 임상증상을 보이며 초기치료 후에 상태가 안정적이더라도 지연성으로 임상적 또는 신경학적 악화가 발생할 가능성이 있으므로 치료에 유의하여야 한다.

• The Journal of the Korea Contents Association
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• v.15 no.6
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• pp.529-538
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• 2015

This study was carried out to evaluate the acute oral toxicity of Corydalis turtschaninovii BESS. in Sprague-Dawley(SD) rats. Male and female rats were administered orally with Corydalis turtschaninovii BESS water extract. We measured the number of death by clinical signs and gross findings for 7 days. After 7 days, we measured the whole body and individual organs' weight. We also analyzed hematological changes. The result, no dead SD rats and no clinical signs were found during the experiment period. Also other specific changes were not found between control and treated groups in hematology and serum biochemistry. The results indicated that there were no significant changes of gross body and individual organs weight in SD rats. These results suggest that water soluble extract of Corydalis turtschaninovii BESS. has not acute oral toxicity in SD rats.

• Proceedings of the Korean Statistical Society Conference
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• 2004.11a
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• pp.111-116
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• 2004

스트레스 수준이 논을 것으로 예상되는 공군사관학교 수험생의 신체검사 결과로부터 신체건강 지표인 혈액 내 생화학적 요인들과 정신건강 지표인 다면 인성검사 점수를 추출하여 통계적 상관성 및 유의성을 비교 분석하였다 사관생도들 중 $10\%$의 표본을 추출하여 사관생도와 수험생 집단간의 특성 비교를 위한 비교집단으로 이용하였다. 수험생과 사관생도 집단 간의 특성 요인 비교는 Mann-whitney U 검정과 Kruskal-Wallis 검정을 이용하였다. 수험생 집단 내에서 혈중 요인 및 정신건강 지표인 MMPI 점수는 정상범주에 있었고 두 요인 간 상관성은 발견할 수 없었다. 그러나 사관생도와 수험생 집단 간의 특성 요인들에 대한 유의성 검정 결과, 특히 2학년생도들의 혈액 내 혈구 수 및 활동성 또는 적극성과 관련된 MMPI 하위척도에서 수험생 집단과 매운 유의한 차이를 보이는 특이한 현상을 발견할 수 있었고 이에 대한 해석을 언급하였다.