• Applied Biological Chemistry
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• v.28 no.3
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• pp.187-195
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• 1985

Electrophoretic studies of protein extracts from carrot calluses suspension-cultured on the media containing kinetin, BA, IAA, NAA or $GA_3$ at the levels of $10^{-6},\;10^{-5},\;10^{-4}M$, respectively, were performed to identify polypeptides and proteins regulated by auxin, cytokinin or GA. Fifteen bands of polypeptide(s) were observed in the callus cultured in the control medium devoid of growth regulators, and their molecular weights were $18._4,\;20._2,\;20._0,\;34._9,\;35._7,\;37._4,\;40._3,\;42._2,\;44._1,\;44._4,\;49._3,\;55._0,\;56._6,\;58._1,\;and\;59._9\;KD$, respectively. The synthesis of polypeptide appeared to be promoted in two bands by kinetin, in six bands by BA, in one band by IAA, in two bands by NAA, and in four bands by $GA_3$, while inhibited in five bands by kinetin, in three bands by BA, in four bands by IAA, in three bands by NAA and in three bands by $GA_3$. The polypeptides of $40._3\;KD\;42._2\;KD$ seemed to be regulated by cytokinins, and those of $44._1\;KD,37._4\;KD,\;and\;56._6\;KD$ by auxins. The proteins of three bands with relative mobilities of 0.56, 0.84, and 0.92, respectively, increased in the calluses cultured on the media containing kinetin, IAA, $GA_3$, NAA or BA, compared to the control, but it was difficult to identify the proteins specific for each growth regulator.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2003.10a
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• pp.143-151
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• 2003

• Journal of Bio-Environment Control
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• v.10 no.1
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• pp.1-9
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• 2001

Fruit growth in kiwifruit shows double sigmoid curve, but it does not certainly indicate as years. Therefore, I though the reason to be easy to the effect of water state change in kiwifruit, investigated diurnal change in water status of fruit tissues with an isoipiestic psychrometers against the fruit growth stage of kiwifruit in 1995 and 1996. Diurnal change in the fruit tissue water potential were little, but violent for fruti growth state III in 1996. The potential of two years dropped gradually approach to harvest time. On the other hand, osmotic potential of the tissues indicated to very similar to water potential, dropped rapidly -1.5MPa before dawn, recovered -1 MPa after 3 h on October 14, were -1~-1.7 MPa at the fruit commercial harvest in 1995. It had a tendency to lower in 1996 than in 1995. It was recorded to the minimum air temperature at the first for an autumn in 1995; 13$^{\circ}C$ from the middle night of October 13 to dawn of October 14. Leaves water potential, which is related to water status of xylem, nearly fell below -1 MPa at before dawn from stage II in 1996. However, it fell so low only at commercial in 1995. At the stage II, osmotic potential and ascent of the turgor pressure was high than 1995-fruit. There parameter suggested that three of kiwifruit in 1996 were status of water stress for stage III. The results from this study indicated that difference of fruit growth between 1995-fruit and 1996-fruit was affected by water status of the fruit tissues, which was influenced by weather condition.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2007.11a
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• pp.371.1-371.1
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• 2007

• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.6
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• pp.948-956
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• 2013

This study investigated methods to improve the quality of Topokkidduk using grapefruit seed extracts and acid regulation agents. Topokkidduk was investigated in terms of its moisture, color, pH, texture, microbial composition, and sensory properties. The moisture content of Topokkidduk was highest for Topokkidduk supplemented with grapefruit seed extracts. The color of Topokkidduk (the L value) decreased, whereas the "a" value increased with the addition of grapefruit seed extracts. The texture of Topokkidduk was affected more by additives than storage temperature. The addition of grapefruit seed extracts and acid regulation agents were effective for the inhibition of microbial activity in Topokkidduk during storage. The overall acceptability values of Topokkidduk (without supplements) were highest among the samples. These results suggest that grapefruit seed extracts and acid regulation agents can be used to control microbial load and moisture levels in Topokkidduk.

• Korean Journal of Plant Tissue Culture
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• v.22 no.2
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• pp.83-87
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• 1995

Regulation of organ differentiation by growth regulators was investigated through the shoot-tip and bulb-scale cultures of Lilium longiflorum (cv Georgia). When shoot tips were placed on MS medium supplemented with 0.1 mg/L NAA alone or 0.1 mg/L NAA and 0.1 mg/L BA, axillary shoots were proliferated. Root diffentiation and growth were stimulated on the basal medium. Although growth regulation did not seem to be necessary when bulb scales were used as explants for shoot differentiation, its differentiation was promoted vigorously by 0.2 mg/L NAA, but suppressed by BA. Bulblets were formed from bulb-scale-derived plantlets cultured on MS medium supplemented with 0.1 mg/L IBA. And more bulblets were formed from the plantlet in MS medium supplying 0.2 mg/L NAA with 6% than3% sucrose

• Korean Journal of Plant Tissue Culture
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• v.25 no.2
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• pp.115-118
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• 1998

This experiments were carried out to determine the optimum concentrations of plant growth regulators for the direct embryogenesis from the cotyledon culture of Paeonia lactiflora Pall. Zygotic embryos rescued from true seeds were developed abnormally in the medium containing ABA. But somatic embryogenesis from cotyledons of abnormal seedlings was induced more effectively. Also the somatic embryogenesis from cotyledons was promoted in the medium containing ABA. The frequency of embryogenesis was maximum(59.9%) from the cotyledons cultured on MS medium containing 0.5 mg/L ABA on which the frequency of somatic embryos with two cotyledons was 22.6%.

• Journal of Bio-Environment Control
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• v.21 no.2
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• pp.127-133
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• 2012

Inhibition effects on spore germination and mycelia growth for pepper anthracnose fungi (Collectricum acutatum) were investigated in vitro using eco-friendly agricultural materials as well as ecofriendly pesticides. The inhibition effect on mycelia growth of anthracnose fungi is the highest when the anthracnose mycelia were treated with a pesticide (commercial name: Koreayeok) that contains a mixture of Bacillus subtilis and Panibacillus polymyxa, resulting in 100% inhibition of the mycelia growth. Meanwhile, the range of 20~40% inhibition effects on the growth of anthracnose mycelia was observed with other commercial agricultural materials. The significant inhibition effects on spore formation of anthracnose fungus were shown in vitro with two water dispersible pesticides containing sulfur [BTB (100%) and SulfurStar (95.1%)], Koreayeok (95.0%), Borstar (99.0%) containing Bordeaux mixture, and Jihabudea-KM containing Psedomonas spp. (96.1%), respectively. Taken from these in vitro results of inhibiting of the spore germination and mycelia growth together, Koreayeok is the most effective on control of pepper anthracnose disease in vitro. In addition, two water dispersible pesticides containing sulfur (BTB and SulfurStar) and Borstar (99.0%) containing Bordeaux mixture are also significantly applicable to prevent pepper plants from anthracnose disease in vitro. It remains to be determined whether the selected eco-friendly agricultural materials in effective control of anthracnose in vitro can be used to control pepper anthracnose in field.

• Journal of Bio-Environment Control
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• v.28 no.2
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• pp.178-184
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• 2019

Strawberry ($Fragaria{\times}ananassa$) is one of the most important and popular fruit crops in the world, and 'Sulhyang' is one of the principal cultivars cultivated in the Republic of Korea for the domestic market. The growth and flower induction in strawberry is the process which influences directly on fruit bearing and yield of this crop. In this study, effect of benzyladenine (BA), gibberellic acid ($GA_3$), and salicylic acid (SA) on growth and flower bud induction in strawberry 'Sulhyang' was investigated. The 3-week-old runner plants, grown in 21-cell propagation trays, were potted and cultivated in growth chambers with $25^{\circ}C/15^{\circ}C$ (day/night) temperatures, 70% relative humidity (RH), and light intensity of $300{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$ photosynthetic photon flux density (PPFD) provided by white light emitting diodes (LEDs). The runner plants were treated with one of three concentrations, 0 (control), 100, and $200mg{\cdot}L^{-1}$ of BA, $GA_3$, or SA solution. The chemicals were sprayed two times on leaves of runner plants at an interval of two weeks. After 9 weeks the results showed that the application of all chemicals caused reduction of root length and chlorophyll (SPAD) content as compared to the control. The lowest chlorophyll (SPAD) content was recorded in plants treated with $GA_3$. However, the treatment of $200mg{\cdot}L^{-1}$ $GA_3$ promoted leaf area, leaf fresh weight, and plant fresh weight. The greatest flower induction (85%) and number of inflorescences (4.3 inflorescences per plant) were observed in the treatment of $200mg{\cdot}L^{-1}\;SA$, followed by $100mg{\cdot}L^{-1}\;SA$. Overall, results suggest that foliar application of $GA_3$ solution could accelerate plant growth, while foliar application of SA solution could induce hastened flowering. Further studies may be needed to find out the relationship between $GA_3$ and SA solutions treated in a combination, and the molecular mechanism involved in those responses observed.

• Asian Journal of Turfgrass Science
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• v.23 no.2
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• pp.287-294
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• 2009

This study conducted to evaluate the growth characteristics of creeping bentgrass in summer after application of the plant growth regulator, Trinecapac-ethyl, and these data may provide basic information to golf course turf maintenance. The results showed that the shoot density of creeping bentgrass was increased an average density of 1.7 $ea/cm^2$ with the trinexapac-ethyl application, especially about 2 $ea/cm^2$ during the growth retarded period of June and July. The root length increased also in June and August. The visual quality was improved significantly with trinexapac-ethyl treatment all the experimental periods, moreover, the effect was significant by reducing a summer decline stress of creeping bentgrass during the warm and humid period of summer. The green speed was significantly improved by this growth regulator treatment and those effect was prominent during stressed season of late June to mid July. Overall of the result, we found that shoot density, visual quality and green speed of bentgrass green were improved by trinexapac-ethyl treated from early growing season of spring and these effects were continued during summer. It should be very beneficial to manage the bentgrass green in stressed season. In future, the possibility and efficiency of mixture with fungicides and/or fertilizers might be needed. The spring green-up test with trinexapac-ethyl will be followed in next spring.