• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2002.04a
• /
• pp.41-42
• /
• 2002

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 2003.04a
• /
• pp.17-23
• /
• 2003

최근 채소작물의 모종은 주로 플러그 육묘방식으로 생산되고 있다. 플러그묘는 단위면적당 많은 개체 수를 육묘할 수 있고, 일련의 작업을 기계화할 수 있으며 운반이나 취급이 용이하는 등 여러 가지 잇점이 있으나 제한된 용적에 밀식되므로 묘가 연약하고 도장되기 쉬운 문제점이 있다(Bae, 1999; Choi, 2002). 이러한 도장 및 과번무 억제를 위해서 대부분의 육묘장에서는 화학적 생장억제제로를 처리하고 있는데 이는 정식한 이후에도 약효의 지속되기 때문에 초기생육을 지연시키거나 생식생장에 부정적인 영향을 미칠 수 있다(Gent, 1997). (중략)

• Korean Journal of Medicinal Crop Science
• /
• v.4 no.2
• /
• pp.132-138
• /
• 1996

This experiment was carried out to establish a year-round production system of pathogen-free stock through micropropagation in Fritillaria thunbergii as medicinal bulbous plant. The effect of different types of culture method and plant growth regulators, activated charcoal and mannitol on bulblet formation and subsequent growth were investigated. The MS solid medium containing 1. 0 mg/L kinetin and 0. 3 mg/L NAA was effective on bulblet formation and propagation rate compared to liquid and suspension culture. Addition of activated charcoal at 0. 01% to 0. 1% in the medium promoted bulbing of cultured bulblets and shoot formation. Addition of 1% to 2% mainnitol in MS medium was effective on the formation of bulblet and subsequent growth of bulblets compared to control. In addition of inhibitors, $10{\sim}100\;mg/L$ B-9 and Chloromequat had effective to stimulate bulblet growth but their effects were not so much as mannitol treatment. ABA treatment had detrimental effect on survival rate of explant and bulblet formation.

• Korean Journal of Plant Tissue Culture
• /
• v.21 no.3
• /
• pp.131-135
• /
• 1994

This experiment was designed to improve the in vitro production system of apple rootstock M.26 as being influenced by the growth regulators, TDZ, BA, IAA, IBA, zeatin, and GA$_3$. Different levels of potassium humate (KH), known as cytokinin and auxin-like substance, were also supplemented to the MS basal medium along with IBA 0.6 mg/L to find out it effect on root formation in apple rootstock M.26. ID initiate and establish the in vitro multiplication of shoots byway of meristem culture, MS medium added with zeatin 1.0 mg/L was found to be the most suitable, showing the 100% of survival rate of shoot tips. A combination of thidiazuron (TDZ) 0.2 mg/L and NAA 0.5 mg/L promoted the shoot proliferation when shoot tips were used as explants. MS basal medium plus IBA 0.6 mg/L was very effective for root induction, but an addition of potassium humate (250 mg/L) to the medium containing IBA 0.6 mg/L stimulated the induction and proliferation of the rook by far the better.

• Journal of Plant Biotechnology
• /
• v.32 no.2
• /
• pp.129-134
• /
• 2005

Effective micropropagation system via somatic embryognesis was established for a Phytophthora resistant Aralia elata cultivar. Different kinds of growth regulators were needed to induce embryogenic callus with different explant sources. When leaf explants were used, a combination of 2,4-D, TDZ and L-glutamine was needed, whereas when petiole and root explants needed only 1.0 mg/L 2,4-D. Embryogenic callus induction rate under the optimum culture condition was 75.0%, 67.0% and 83.0% from leaf, petiole and root segment, respectively. Somatic embryo germination and plantlet conversion rate appeared to be influenced greatly by various osmoticums. More than 90% of embryos germinated when treated with sucrose, glucose and maltose. However, the highest conversion rate (72%) was recorded on medium with 2% sucrose only. The converted plantlets grew normally on 1/2MS basal medium, were acclimatized on artificial soil mixture and survived more than 95% in the greenhouse condition. The results suggest that the species can be clonally propagated through in vitro culture system via somatic embryogenesis.

• Korean Journal of Plant Tissue Culture
• /
• v.25 no.5
• /
• pp.347-351
• /
• 1998

Plant regeneration via organogenesis from leaf and stipule explants of micropropagated shoots of strawberry (Fragaria $\times$ ananassa cv. Suhong) was achieved. Leaf and stipule explants were detached from shoot-tip cultured shoots and cultured on MS medium with various combinations of BA and NAA under light or dark condition. Shoot regeneration from leaf explant was observed after 3 weeks in culture and was good at the high ratio of BA and NAA among various combination treatments. The highest shoot regeneration frequency from leaf explants was obtained with 1.0 mg/L BA and 0.1 mg/L NAA, in which 31.1% shoot regeneration frequency(1.7 shoots per leaf explant) was yielded. In case of stipule explants, shoot regeneration was largely affected by plant growth regulators during incubation under dark condition for initial 4 weeks but not under continuous light condition. The combination treatment with 2.0 mg/L BA and 0.1 mg/L NAA showed the most excellent shoot regeneration from stipule explants, where 44.4% regeneration frequency(4.0 shoots per explants) obtained. Regenerated shoots were rooted on MS medium with 0.1 mg/L NAA after shoot elongation, and the plantlets regenerated were transferred to soil mixtures with vermiculite and perlite for acclimation.

• Korean Journal of Plant Tissue Culture
• /
• v.27 no.3
• /
• pp.197-202
• /
• 2000

The effect of cultural media and growth regulators on multiple plant regeneration from leaf explants of Heloniopsis orientalis (Thunb.) Tanaka was evaluated. The highest percentage of shoot and root formation were 20 and 30% on MS medium treated at 3.0 mg $l^{-1}$ of zeatin, respectively. Also 67 and 33% of high shoot formation appeared on 1/2 MS and 5 culture medium treated at zeatin 1.0 and 3.0 mg $l^{-1}$ respectively. With MS treated at 0.5 mg $l^{-1}$ of 2,4-D 1/2 MS and B5 culture media treated at each 1.0 and 3.0 mg $l^{-1}$ of zeatin the highest ratios of plant produced were 100, 280 and 310 % respectively relative to the other treatments. Generally, there was highest possibility for multiple propagation of Heloniopsis orientalis (Thunb.) C. Tanaka with B5 culture media supplemented 3.0 mg $l^{-1}$ of zeatin.

• FLOWER RESEARCH JOURNAL
• /
• v.19 no.4
• /
• pp.206-211
• /
• 2011

This study was carried the effect of transportation conditions and plant growth regulator on the maintenance of freshness and quality of cut Chrysanthemum 'Baekma'. The quality of cut chrysanthemum 'Baekma' which were stored at $5^{\circ}C$ was better than those of stored at $20^{\circ}C$ and control. When cut mum 'Baekma' was transported stacking boxes with vertical condition after dipping in distilled water, flowering degree, flower size, days to flower appearing degree, and bent neck were delayed. Flowering degree was delayed by $10mg/L^{-1}$ uniconazole spraying, The days to bent neck was delayed by 1,000x daminozide spraying.

• Proceedings of the Korean Society for Bio-Environment Control Conference
• /
• 1997.11a
• /
• pp.91-93
• /
• 1997

근래에 들어서 관상용 허브식물과 허브 가공용품에 대한 수요가 나날이 증가하고 있으나 아직까지 우리나라에서는 체계적인 연구가 미흡한 실정이다. 특히 영양번식이 효과적인 허브식물은 생장조절제 처리에 대한 보다 다양한 실험이 요구되고 있다. 따라서 본 실험에서는 삽목 재배시 IBA처리 효과를 조사하였고, 아울러 수경재배법확립을 위하여 질소원 처리농도 및 형태에 따른 생장율을 비교하였다. (중략)

• Korean Journal of Plant Resources
• /
• v.24 no.2
• /
• pp.168-173
• /
• 2011

This study was carried out to optimize the embryogenic callus induction and plant regeneration from mature seeds of Sorghum bicolor. The effect of growth regulators was investigated on formation of embryogenic callus. The highest frequency of embryogenic callus was observed when the mature seeds were cultured on B5 medium supplemented with 2 mg/L 2,4-Dichlorophenoxyacetic acid (2,4-D). The highest frequency of plant regeneration from embryogenic callus was observed on MS medium with 0.5 $mg\;l^{-1}$ 6-benzyl amino purine (BAP) and 0.25 $mg\;l^{-1}$ indole-3-butyric acid (IBA) to optimize the shoot regeneration. High concentration of BAP (1 $mg\;l^{-1}$) supplemented with IBA (0.25 $mg\;l^{-1}$) was effective combination for shoot multiplication. MS medium supplemented with 1 $mg\;l^{-1}$ IBA was found to be the most effective for inducing roots. Normal rooted plantlets were transferred to the greenhouse for hardening with over 90% survival rate. Hence, this reproducible protocol could be useful for mass propagation and genetic transformation of S. bicolor.