• Title/Summary/Keyword: 생장패턴

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포복성 산호조류, 납작돌잎 (Lithophyllum yessoense)의 생장과 사분포자 방출 패턴

  • 황은경;김은진;김형근;손철현
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2001.10a
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    • pp.271-272
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    • 2001
  • 최근 우리 나라의 연안은 갯녹음으로 황폐화되고 있으며, 이로 인하여 유용 수산자원이 감소하고 있는 실정이다. 이러한 갯녹음 현상은 서식지 환경요인들이 복합적으로 작용하고 있는 생태계의 변화로 연안의 암반은 산호말류로 피복되며 이들이 죽어서 하얗게 변하는 현상(정치ㆍ추강, 1980)으로서, 해중림과 산호말류 군집이 서로의 극상을 반복하는 생태적 현상으로 설명되기도 한다(중구, 1996). 갯녹음 지역의 유용 해중림 복원과 관련하여 갯녹음의 원인생물로 알려져 있는 포복성 산호조류의 종류와 그 생태를 파악하는 것은 무엇보다 시급한 과제이며 산호조류의 포자방출 패턴은 파악하는 것은 해중림 복원을 위한 효과적인 인공어초 투입의 시기를 결정하는데 중요한 요인이 된다. (중략)

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Genetic Stability Analysis of in vitro Regenerated Wolly Grass (Imperata cylindrica 'Rubra') Based on Inter-Simple Sequence Repeats (ISSR) Markers (ISSR 표지에 의한 기내재생 홍띠(Imperata cylindrica 'Rubra')의 유전적 안정성 분석)

  • Ye-Jin Lee;In-jin Kang;Chang-Hyu Bae
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.54-54
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    • 2020
  • 지구온난화에 따라 농업부문 신재생에너지의 중요성이 증대되고 있으며, 화본과 식물은 바이오에너지작물의 중요한 소재를 제공하고 있다. 화본과 식물의 기내대량증식연구의 일환으로 홍띠식물의 기내 재생 식물체의 유전적 안정성에 대한 기초자료를 제공할 목적으로 기내배양으로 재분화시킨 홍띠(Imperata cylindrica 'Rubra') 재분화 식물체 중 녹색체 재생식물체를 대상으로 ISSR 표지를 사용하여 유전적 안정성을 조사하였다. 재분화식물체는 MS (Murashige and Skoog, 1962)배지에 생장조절제를 첨가한 배지에서 배양하였다. 생장점 부위를 적출하여 캘러스를 유도하고(0.1 mg/L 2,4-D와 2 mg/L BA), 캘러스 증식(0.1 mg/L 2,4-D와 0.05 mg/L BA), 신초 재분화( 0.01 mg/L NAA와 2 mg/L BA) 후 MS배지에서 식물체를 양성하고 순화시켰다. 배양은 26±2℃, 25 µmol/m2/s, 14h/10h (day/night) 광조건 하에서 실시하였다. 재분화식물체는 홍띠 및 녹색 재분화식물체 2 종류로 나타났는데, 이는 생장점에서는 홍띠가 분화되었음에도 불구하고 생장점 주변조직에서 유래한 녹새체가 분화된 후 우세하게 자라서 녹색재생체가 우점하는 것으로 추정된다. ISSR 분석은 대조구로 모식물체 홍띠를(8개체), 재분화식물체는 녹색체 중, 1년간 노지포장에서 재배중인 녹색체(10개체)와 실험실내 화분에서 재배중인 시료를(10개체) 사용하였다. ISSR 밴드패턴을 비교한 결과, 재분화체는 실내포트 재배식물체 10.3%, 노지1년 재배식물체 8.3%로 대조구의 4.1%보다 유전적 다형성 비율이 2배 이상 높게 나타났다. 또한 재분화식물체들의 유전적 유사도를 평가하고 군집분석을 실시하였다.

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Effects of Aluminium on Growth, Chlorophyll Content, ALAD Activity and Anatomy of Root rind Shoot in Azuki Bean (Vigna angularis) Seedlings (Aluminium이 팥(Vigna angularis) 유식물의 생장, 엽록소함량, ALAD활성 및 뿌리와 경엽부의 형태에 미치는 영향)

  • 구서영;홍정희
    • Journal of Environmental Science International
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    • v.5 no.6
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    • pp.813-826
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    • 1996
  • The toxic effects of aluminium (Al) on growth, chlorophyll content, $\delta-aminolevulinic$ acid dehydratase (ALAD) activity and anatomy of root and shoot were investigated in 7-day-old azuki bean (Vigna angularis) seedlings. Significant depressions in root elongation was observed in the low concentrations of Al (50, 100 $\muM)$ and increasing Al concentrations caused a sharp decline of root and shoot growth. The degree of inhibition was dependent upon Al supply. Exposure to 50 $\muM$ Al or more inhibited root elongation within 1 day. In the 50 $\muM$ Al treatments, a recovery of root growth was seen after 7 days exposure. In contrast, lateral root initials was little affected by Al exposure. Al toxicity symptoms and growth responses were more well developed in the roots than in the shoots. Analysis of Al localization in root cells by hematoxylin stAlning showed that Al entered root apices and accumulated in the epidermal and cortical cells immeadiately below the epidermis. There was a good positive correlation between the level of chlorophyll and ALAD activity. Increasing Al concentrations caused a decrease in total chlorophyll contents, accompanied by proportional changes in ALAD activity, suggesting a cootr-dinated reduction of a photosynthetic machinery. Al exerted specific influence on the morphology of root ann shoot. At higher concentrations of Al the roots induced drastic anatomical changes. The epidermal cells were disorganized or destructed while the cortical cells exhibited distortion of cell shape and/or disintegration. The diameter of root and transectional area of cortical cells decreased considerably with Al treatment. In the shoot Al also enhanced reduction of diameter of shoot and cell size. Gross anatomy of leaves treated with Al did not differ significantly from the controls, except for fewer and smaller chloroplast. Our results indicate that toxic effect of Al appear to be manifested primarily in roots and secondarily on shoots, and changes in root morphology are related to changes in the root growth patterns. Results are further discussed in re181ion to the findings in other plant species, and it is concluded that Al causes morphological, structural and, presumably, functional damage to the roots of the species investigated.

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Growth characters and harvest time for the artificial cultivation of Mycoleptodonoides aitchisonii (침버섯 인공재배 생육 특성과 수확 최적 시기)

  • Kim, Young;Jung, Bo-Mi;Wi, An-Jin;Park, Whoa-Shig;Bang, Mi-Ae;Park, Dae-Hun;Seo, Joung-Wook;Oh, Deuk-Sil
    • Journal of Mushroom
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    • v.13 no.2
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    • pp.114-118
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    • 2015
  • Mycoleptodonoides aitchisonii has been used a culinary material and traditional medicine for a long time in worldwide and recently the researches to find biological effects have been increased such as dopamine activation, preventive effect against phytopathogens, inhibitive effect against erythrocyte coagulation, anti-oxidative effect, anti-tumorigenic effect, etc. However it is hard to cultivate Mycoleptodonoides aitchisonii it is impossible to be mass-produced and in order to solve the problem in this study we found the appropriate cultivation period and the harvest point for it. For life cycle (from primordium formation to harvest) the morphology, weight, and quantity of polysaccharide of Mycoleptodonoides aitchisonii were measured using with 5 bodies per a day and it could be divided for 4 stages; primordium formation, growth, needle maturation, and aging. And then from the results the Zeide nonlinear growth curve could be gotten. At 13th day after cultivation there is the relation between the change of media weight and body weight and at 14th day after cultivation the rate of polysaccharide in the body was 11 %. However in the case of O2 insufficient supply the malformation of them was observed.

Effects of Paper Mill Sludge-Fertilizer Application on the Growth Performances of Tree Seedlings (제지(製紙)슬러지 가공비료(加工肥料)의 시용(施用)이 수묘(樹苗)의 생육(生育)에 미치는 영향(影響))

  • Kwon, Ki-Won;Lee, Kyu-Seung
    • Korean Journal of Environmental Agriculture
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    • v.12 no.3
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    • pp.219-229
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    • 1993
  • Effects of paper mill sludge-fertilizers were investigated on the growth performances of tree seedlings under field conditions. Two types of sludge-fertilizers applied were an organic sludge compost and a processed sludge-pellet fertilizer strengthened with several components of organic and inorganic nutrients. Three species of tree seedlings studied were ibota privet(Ligustrum obtusifolium) and yellow poplar(Liriodendron tulipifera) of 1-0 year seedlings and also eastern white pine(Pinus strobus) of 2-2 year seedlings. Nitrogen and phosphorus contents of plot soil treated with sludge compost+sludge-pellet fertilizer+sludge-pellet fertilizer plot were increased to 8 times and 2.6 times as those of control plot, respectively. The growth performances of root collar diameter and seedling height were repeatedly measured up to five times through the growing season and the results were analyzed statistically by analysis of variance of randomized block design and Duncan's multiple range test. The growth performances of dry weight were measured after last fifth measurements.

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Appropriate Each Irrigation Quantity in Irrigation System Controlled by Drainage Level Sensor for Perlite Bag Culture of Tomato (배액전극제어법을 이용한 토마토 펄라이트 자루재배시 일회급액량 구명)

  • Kim, Sung-Eun;Sim, Sang-Youn;Lee, Sang-Don;Kim, Young-Shik
    • Horticultural Science & Technology
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    • v.29 no.1
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    • pp.36-42
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    • 2011
  • This research was conducted to investigate the effects of irrigation quantity in irrigation management system controlled by drainage level sensors for perlite bag culture on the growth and yields of tomatoes during different growth stages. Tomato plants were irrigated with four selected methods; supplying small quantity (~70 mL) during entire growth (S-S), large quantity (~145 mL) during entire growth (L-L), small quantity before harvesting the first cluster fruits and large quantity after harvesting (S-L), and large quantity until harvesting the first cluster fruits and small quantity after harvesting (L-S). The irrigation quantity supplied in each time was gradually adjusted along with the ratios as the tomato crop grew during different growth stages. The growth of the tomato plants was unstable and slow during the entire cropping period when the plant was irrigated by small or large quantities (S-S or L-L). In L-S treatment, the growth phase of the tomato was changed from vegetative to generative growth on the basis of the plant development index when each irrigation quantity was changed. The L-S treatment exhibited the largest root volume and yields with stable drainage ratios. Therefore, the optimum irrigation quantity was determined as 145 mL before harvesting the first cluster fruits and 70 mL after harvesting.

Uptake of Isolated Lyophyllum ulmarium Chromosomes by Ganoderma applanatum Protoplasts (잔나비걸상버섯 원형질체(原形質體)에 의(依)한 만가닥버섯 염색체(染色體)의 섭입(攝入))

  • Yoo, Young-Bok;You, Chang-Hyun;Chang, Kwon-Yawl
    • The Korean Journal of Mycology
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    • v.16 no.4
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    • pp.247-252
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    • 1988
  • The uptake of isolated Lyophyllum ulmarium chromosomes by Ganoderma applanatum protoplasts was induced with polyethylen $glycol+CaCl_2+glycine$. Uptake products of chromosomes by protoplasts showed micro-and macrotransgenome type. The former was slowly growing and unstable, the latter was outgrowing and stable mycelial colonies which made thick hyphae of width and segregation of mycelial colony on GCM containing benomyl. A comparison of macrotransgenome type was using isozyme analysis of esterase. The enzyme pattern of two transformants was distinct in position and quantity compared with parents.

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Development of Capacitive Sensing Based Self-sustainable Water Monitoring Sensor Node for Plant Growth Management (정전용량 센싱기반 식물생장관리용 자기유지 지원 수분 모니터링 센서노드 설계)

  • Song, Min-Hwan;Lee, Sang-Shin;Won, Kwang-Ho
    • Proceedings of the Korea Information Processing Society Conference
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    • 2012.04a
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    • pp.986-988
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    • 2012
  • 최적의 식물 생장을 위해서는 적절한 수분의 유지가 필수적이며 넓은 지역, 다양한 종류, 고가의 식물의 경우일수록 적절한 수분의 관리를 위한 시스템의 도움이 필요하다. 이를 위해 저가의 센서노드 시스템이 적절한 해결책이 될 수 있으나 일반적인 배터리 기반의 센서노드 시스템을 적용시 배터리 용량 확인 및 교체 등의 유지보수 문제가 대두된다. 본 논문에서는 이러한 유지보수의 문제를 해결하고 식물재배에 도움을 줄 수 있는 자기유지 지원 방식의 정전용량 센싱기반의 수분 모니터링 센서노드를 설계하였다. UHF 기반의 무선 전력 전송의 자기유지 지원 시스템 및 PCB 패턴기반의 정전용량센싱 수분센서와 초저전력 센서노드 시스템으로 구성된다. 센서노드는 한번 송신시 약 0.24 mJ을 소모하며 에너지획득모듈은 에너지 획득 주기마다 약 4 mJ의 에너지를 공급하도록 설계하여 센서동작을 위한 충분한 에너지 마진을 주도록 설계하였다.

Growth Potential of Escherichia coli O157:H7 in Lettuce Leaf Extract and on Lettuce Leaf Surface (상추잎 즙액과 표면에서 Escherichia coli O157:H7의 생장 가능성)

  • Kim, Won-Il;Jung, Hyang-Mi;Kim, Se-Ri;Park, Kyeong-Hun;Kim, Byung-Seok;Yun, Hye-Jung;Yun, Jong-Chul;Ryu, Kyoung-Yul
    • Journal of Food Hygiene and Safety
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    • v.26 no.4
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    • pp.296-301
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    • 2011
  • This study was to evaluate the growth potential of E. coli O157:H7 in lettuce leaf extracts and on lettuce leaf surface at various temperatures. The pathogen can survive and multiply in the extracts and leaf surface of lettuce. The population of E. coli O157:H7 in the lettuce extracts reached to 4.79 log CFU/mL at $37^{\circ}C$. The multiplication of pathogen in lettuce extracts initiated within 10 hours of inoculation over $15^{\circ}C$ conditions. And it can survive in the lettuce leaf extracts at $4^{\circ}C$ for 100 hours at least. And this pathogen can multiply on lettuce leaf surface and the population of pathogen on the lettuce leaf surface increased to 1.82 log CFU/g at $25^{\circ}C$. At $37^{\circ}C$, the pathogen density increased to 1.53 CFU/g within 3 days after inoculation. At all temperature, irrespective of the inoculation level, similar trends in growth of E. coli O157:H7 were observed. These results emphasize the growth potential of E. coli O157:H7 in lettuce leaf extract and on lettuce leaf surface. To reduce the risk of outbreak, it is important to maintain the cold chain system during storage before the consumption.

Effect on Quality Change of Cherry Tomato by $CO_2$ Concentration of Flushed Gas and Storage Period (충전가스의 $CO_2$ 함량 및 노출기간의 변화가 방울토마토의 품질변화에 미치는 영향)

  • Lee, Seung Yuan;Lee, Seung Jae;Choi, Dong Soo;Hur, Sun Jin
    • Culinary science and hospitality research
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    • v.20 no.6
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    • pp.200-210
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    • 2014
  • The purpose of this study was to investigate the quality changes and contamination of microorganisms such as Escherichia coli, mold and yeast in cherry tomatoes during storage at different temperatures, gas composition and periods(7 and 14 days). This study determined pH, color change and the growth pattern of microorganisms in cherry tomato during storage at $5^{\circ}C$, $10^{\circ}C$ and $15^{\circ}C$. According to the results, pH level was a little raised with storage period. On average, $L^*$, $a^*$ and $b^*$ value of cherry tomato were irregular value of increase and decrease of all gas packaging with storage period. In regard of the types of microorganism, aerobic count plate, coliform count, mold and yeast were detected when cherry tomatoes were stored at $5^{\circ}C$, $10^{\circ}C$ and $15^{\circ}C$ during storage for 14 days. Equally, all microorganisms of cherry tomato were irregular with storage period and complex gas packaging. However, this study determined that packaging with a higher $CO_2$ concentration than $O_2$ concentration can reduce growth of microorganism. These studies can be used as primary data for determining the optimal complex gas to storage enlargement.