• Title/Summary/Keyword: 생육저해

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가죽나무 및 할미꽃으로부터 식물생장억제활성물질의 탐색

  • 김건우;김진석;조광연;홍경식;최정섭;신준구;백정규;이윤칠
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2002.11b
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    • pp.27-27
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    • 2002
  • 식용피(Echinochloa crus-galli var. formosensis Ohwi.) 유묘에 대한 생육저해 정도를 지표로 하여 경북 북부지방에 자생하는 10여종의 야생식물의 부위별 MeOH 추출물을 조사한 결과, 1,000 ppm에서 가죽나무(가지)및 할미꽃(경엽부)은 식용피 유묘의 뿌리의 생육을 약 60∼80% 억제시키는 것으로 나타났다. 식물생장억제활성이 나타난 상기 2종에 대해 각종 크로마토그래피법을 적용하여 활성의 본체를 분리ㆍ정제한 결과, 가죽나무 가지 및 할미꽂 경엽부로부터 500 ppm에서 식용피 유묘 뿌리의 생육을 각각 96%와 73% 억제하는 활성분획들이 획득되었으며, GC/MS 분석을 통해 이들 활성분획으로부터 수종의 식물생장억제활성물질들이 확인되었다.

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Microbiological Characteristics of Whitening Tyrosinase Inhibitor-producing Wild Yeasts, Saccharomyces cerevisiae WJSL0191 and Papiliotrema laurentii ON30 and Production (미백성 Tyrsoinase 저해물질 생산 비병원성 야생효모 Saccharomyces cerevisiae WJSL0191와 Papiliotrema laurentii ON30의 균학적 특성과 Tyrosinase 저해물질의 생산)

  • Kim, Ji-Yoon;Lee, Jong-Soo
    • The Korean Journal of Mycology
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    • v.48 no.3
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    • pp.285-296
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    • 2020
  • The goal of this study and potent whitening tyrosinase inhibitor-producing wild yeasts and further optimiz production of tyrosinase. Among non-pathogenic wild yeasts obtained from soils in Daejeon city and spice field of Geumsan in Chungcheongnam-do, Korea, we selected Saccharomyces cerevisiae(S. cerevisiae) WJSL0191 and Papiliotrema laurentii (P. laurentii) ON30 show 33.2% and 27.3% respectively. These selected strains formed and not pseudomycelium. S. cerevisiae WJSL0191 was sugar-tolerant as well as halophilic in 20% glucose-containing yeast (YPD) medium and 15% NaCl-containing YPD medium. S. cerevisiae WJSL0191 and P. laurentii ON30 showed 26.2% and 18.6% anti-wrinkle elastase inhibitory activities, respectively. aximal production of tyrosinase inhibitors obtained when S. cerevisiae WJSL0191 was cultured at 30 for 72h in YPD medium and P. laurentii ON30 was incubated at 20℃ for 24hr.

Purification of Vibrio anguillarum Growth Inhibition Factor Produced by Bacillus amyloliquefaciens H41. (Bacillus amyloliquefaciens H41이 생산하는 Vibrio anguillarum 생육 저해인자의 정제)

  • Shin, Hyun-Chul;Chung, Kyung-Tae;Kim, Kwang-Hyun;Kim, Byung-Woo;Kwon, Hyun-Ju;Lee, Eun-Woo;Yum, Jong-Hwa;Rhu, Eun-Ju;Jeong, Yu-Jeong;Kim, Young-Hee
    • Journal of Life Science
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    • v.18 no.6
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    • pp.789-795
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    • 2008
  • To study the possible use of probiotics in fish farming, we evaluated antagonism of antibacterial strain Bacillus amyloliquefaciens H41 against the fish pathogenic bacterium Vibrio anguillarum NCMB1. The purification of growth inhibition factor produced by B. amyloliquefaciens H41 was achieved by obtaining supernatant of this bacterium. The growth inhibition factor was purified to homogeneity by 70% ammonium sulfate precipitation, DEAE-sephadex A-50 ion exchange chromatography, sephadex G-200 gel filtration column chromatography, and sephadex G-50 gel filtration column chromatography with 40.8 fold of purification and 2.9% yield. The molecular weight of the purified growth inhibition factor was 48 kDa as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH and temperature for the growth inhibition factor were pH 7.5 and $30^{\circ}C$, respectively. The activity of growth inhibition factor was enhanced slightly by some metal ions, such as $Mg^{+2}$, $Mn^{+2}$, but was inhibited by the addition of $Co^{+2}$, $Hg^{+2}$, $Zn^{+2}$ and $Ag^{+2}$. NaCl stability of the growth inhibition factor was observed with 50% residual activity at 3% NaCl concentration. Toxicity test showed that the purified B. amyloliquefaciens H41 growth inhibition factor did not affect the live of Japanese flounder (Paralichthys olivaceus) and the effectiveness was 78% of residual lethality compared to commercial antibacterial agents.

Effects of Low Dose $\gamma$-Radiation on the Growth, Activities of Enzymes and Photosynthetic Activities of Gourd (Lagenaria siceraria) (저선량 $\gamma$선 조사가 참박의 초기 생육과 효소 활성 및 광합성 능에 미치는 영향)

  • 이혜연;김재성;백명화;이영근;임돈순
    • Korean Journal of Environmental Biology
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    • v.20 no.3
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    • pp.197-204
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    • 2002
  • Gourd seeds were irradiated with the doses of 0-20 Gy to investigate the effect of the low dose $\gamma$-radiation on the early growth and physiological activity. The stimulating effects of the low dose y- radiation on the early growth were not noticeably high, but were increased generally at 4-16 Gy irradiation group. The catalase and peroxidase activity of cotyledon from seeds irradiated with $\gamma$- radiation were increased at 8 Gy irradiation group. The photochemical activity of leaf was noticeably high at 4 Gy irradiation group. The photochemical yield of PSII, estimated as Fv/Fm, decreased with increasing illumination time by 50% after 4 hrs in the control and 8 Gy irradiation group, while Fo slightly increased. However, Fv/Em in the 4 Gy irradiation group decreased by 40% of inhibition, indicating that photoinhibition decreased by the low dose $\gamma$- radiation. Changes in the effective quantum yield of PSII, $\varphi_{PSII}$ and 1/Fo- l/Fm, a measure of the rate constant of excitation trapping by the PSII reaction center, showed similar pattern to Fv/Em. NPQ decreased by 70% after photoinhibitory treatment with showing similar pattern between the control and the irradiation group. These results showed the positive effect of low dose $\gamma$- radiation on the seedling growth and the reduction of photoinhibition in the 4 Gy irradiation group.

Effect of Spatial Soil Salinity Variation on the Growth of Soiling and Forage Crops Seeded at the Newly Reclaimed Tidal Lands in Korea (신 간척지토양의 공간적 염농도 변이가 녹비·사료작물의 생육에 미치는 영향)

  • Sohn, Yong-Man;Jeon, Geon-Yeong;Song, Jae-Do;Lee, Jae-Hwang;Park, Moo-Eon
    • Korean Journal of Soil Science and Fertilizer
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    • v.42 no.3
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    • pp.179-186
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    • 2009
  • Relation between soil salinity and forage yield of five upland crops such as sudan grass, sesbania, barnyard grass, corn and soybean was studied in the three reclaimed lands of Korea during two years from 2007 to 2008. Although plant number emerged was obtained satisfactory by desalting treatment, further growth of crops was sharply affected by re-salting process according to soil drying. Soil salinity varied place by place and changed constantly, crop growth was differently responded to soil salinity according to the specific conditions of the reclaimed lands. In the Iweon reclaimed land with higher soil water conductivity and sandy soil texture, crop growth and yield sharply decrease with increase of soil salinity. Relation between soil salinity and crop growth and yield was well expressed as logarithmic function. Surface soil EC to reach at 50% of growth reduction to the tallest height of crops was $5dS\;m^{-1}$ for soybean, $6dS\;m^{-1}$ for corn and $7dS\;m^{-1}$ for sudan grass, sesbania, and barnyard grass by logarithmic function. In the Hwaong and Yeongsangang reclaimed lands with low soil conductivity and finer soil texture, plant growth response to salt stress was statistically vague by mixing of harmful influence from flooding and wet injury. However, it is observed that crop growth and yield on the place of lower salinity was better than crop growth on the place of higher salinity. It is accordingly concluded that flooding control during summer rainy season is vary important as well as desalting process for good growth of soiling and forage crops in the newly reclaimed land from tidal flat.

Antimicrobial Activity of Maesil(Prunus mume) Juice against Selected Pathogenic Microorganisms (매실(Prunus mume) 착즙액의 식중독 유발균에 대한 항균 작용)

  • 이현애;남은숙;박신인
    • The Korean Journal of Food And Nutrition
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    • v.16 no.1
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    • pp.29-34
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    • 2003
  • Prunus mume has been used for the folk medicine by many old civilizations to treat food-borne diseases or enteric disorders. This study was carried out to examine the antimicrobial effect of juice from Prunus mume against pathogens such as Escherichia coli, Salmonella enteritidis, Staphylococcus aureus, Listeria monocytogenes and Bacillus cereus. The juice of Prunus mume had the strongest antimicrobial activity to Sal. enteritidis. The concentrations of Prunus mume juice for the formation of clear zone were 1% for Sai. enteritidis(15.0mm), 3% for Lis. monocytogenes(14.7mm), and 5% for Bac. cereus(14.75mm), Esc. coli(13.45mm) and Sta. aureux(11.9mm). The growth of all tested microorganisms was inhibited apparently in tryptic soy broth containing 3% and 4% Prunus mume juice. And it was found that the Prunus mume juice showed the highest antimicrobial properties, followed by Sal. enteritidis, Bac. cereus, Sta. aureus, Lis. monocytogenes, Esc. coli.

Antioxidant and Antimicrobial Activities of Various Berry Juices (베리류 열매 착즙액의 항산화 및 항균 활성)

  • Nam, Jin-Sik;Han, Young-Jin;Yeo, Soo-Min
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.328-334
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    • 2015
  • This study was performed to evaluate and compare the antioxidant and antimicrobial activities of various berry juices (cherry, grape, blueberry, aronia, cranberry, and raspberry). The cherry, aronia, and cranberry juices included higher levels of total polyphenol than the other berry juices. The DPPH radical scavenging activity of various berry juices increased according to the juices concentration. The scavenging activity of DPPH radicals by the aronia, cranberry, and raspberry juices was higher than those of other samples and was in the following order: aronia > cranberry > raspberry. The FRAP values of 100% aronia juice was the highest among the samples, which was more than five times higher than grape juice at a concentration of 25%. The cranberry and raspberry juices exhibited a strong inhibitory effect against important food-borne bacteria, and in general, the berry juices inhibited the growth of Gram-negative bacteria more than that of Gram-positive bacteria. The cranberry and raspberry juices exhibited high antimicrobial activities against important food-borne bacteria at a concentration of 100% and 75%, however, they did not affect food-borne bacteria at a concentration below 10%. These results suggest that aronia, cranberry, and raspberry may be used effectively as natural additives and as functional foods due to their high antioxidant and antimicrobial activities.

A Study on the evaluation of antimicrobial activity of extracts from Rhus javanica L fruit (붉나무 열매 추출물의 항균효능 평가에 관한 연구)

  • Jang, Deok-Young;Yang, Jae-Chan
    • Journal of the Korean Applied Science and Technology
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    • v.37 no.1
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    • pp.145-152
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    • 2020
  • In this study, the antimicrobial activity was tested by Ethanol extract(ET), Ethyl acetate fraction(EA) and Butanol fraction(BT) of Rhus javanica L fruit as natural preservatives. The antimicrobial activity were tested by Paper disc method and minimum inhibitory concentration (MIC) for microorganisms (Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Pseudomonas aeruginosa, Candida Albicans). As a result of the antimicrobial activities of P. aeruginosa fruit extracts have shown the clear zone that S. aureus, S. epidermidis, E. coli, and P. aeruginosa. In BT, additional clear zones were observed for the Candida. The MIC results showed that EA samples showed the lowest concentrations for S. aureus S. epidermidis, E. coli, and P. aeruginosa. Accordingly, it can be concluded that these Rhus javanica L fruit extracts have the potential for antimicrobial materials for the cosmetic industry.

Inhibitory Effect of Cephalosporin C on Growth of Cephalosporium acremonium M-113 (Cephalosporium acremonium M-113의 세팔로스포린에 의한 생장억제 효과)

  • Kim, Myung-Kuk;Park, Sang-Ho;Lee, Jeong-Kug;Kho, Yung-Hee;Mheen, Tae-Ick
    • Microbiology and Biotechnology Letters
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    • v.13 no.4
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    • pp.339-344
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    • 1985
  • Cephalosporin C(CPC) inhibited the growth of Cephalosporium acremonium M-113, a potent CPC producer derived from C acremonium ATCC 20339. Similar inhibitory effects of CPC were also observed in growth of C. acremonium ATCC 20339 and ATCC 14553. Minimum inhibitory concentrations (MIC) of CPC on the growth of conidia and hyphae of C. acremonium M-113 were 200-500 and 3000-4000$\mu\textrm{g}$/$m\ell$ respectively in synthetic medium. MIC values were increased in complex media. The inhibitory effect of CPC was due to CPC-exerted inhibition of amino acids uptake by the cells. 3'-Group of CPC might be important in its inhibitory action. In audition, CPC itself could be utilized by the cells as a nitrogen source under nitrogen limited condition.

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