• 한국생물공학회:학술대회논문집
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• 2005.10a
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• pp.730-734
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• 2005

Flow injection analysis (FIA) system was developed to monitor glucose concentrations in biotechnological processes. A fiber optic oxygen sensor was used to determine consumption of oxygen concentration by reaction of immobilized glucose oxidase (GOD). The GOD was immobilized on VA-Epoxy carrier and integrated into FIA system. A calibration curve for glucose was obtained in the range of 0.5 $g/L{\sim}3.0$ g/L.

• Journal of the Korea Institute of Military Science and Technology
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• v.27 no.5
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• pp.649-656
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• 2024

This report describes the test results of decontamination of Bacillus atrophaeus spores. The spore solution at 10⁹ cfu/mL concentration was treated with chemical decontaminants, hydrogen peroxide, DF-200, sodium dichloroisocyanurate(NaDCC), and perasasfe. DF-200 was not suitable for decontamination of the spore solution. Among them, the optimal decontamination reaction conditions of perasafe and NaDCC were established from test tube experiments in a mL-scale, and they were validated in a bio-reaction system in a L-scale. The optimal conditions of perasafe and NaDCC were 1.0 % of concentration with 5 minutes of reaction time and 0.3 % of concentration with 10 minutes of reaction time, respectively. Also, both decontaminants required adequate agitation for decontamination. The results in the bio-reaction system were similar with the results from the smaller scale using test tubes, showing that the optimal conditions from the smaller scale experiments can be applied to the larger scale.

• KSBB Journal
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• v.8 no.4
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• pp.336-340
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• 1993

Zoogloea ramigera 115, well known type of bacteria to produce slime in sewage plants, was selected for biopolymer production. The extracted biopolymer showed high uptake capacity of metals such as cadmium and zinc. Especially the fermentor broth itself showed high adsorption of metal and could be used a biosorbent without an additional separation process. Biopolymer was immobilized into beads of calcium alginate and used in a packed bed reactor for the purpose of valued metals recovery. The biopolymer showed high removal efficiencies of 80% or greater for Cu, Cd, Mn and Zn, and high stability in sorption-desorption-resorption experiments. The immobilized biopolymer systems were found to be comparable to other metal removal systems such as ion exchange resins and to be of potential industrial application value.

• KSBB Journal
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• v.5 no.2
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• pp.133-139
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• 1990

A simple and convenient method was introduced to determine the kinetic parameters for various enzymatic reaction kinetics. The method based on integrated formular can be applied to the parameter estimations from a single experiment. A modified three-parameter model was applied for the parameter estimation in reversible reaction and the equilibrium substrate concentration could be also estimated. It is possible to identify the enzymatic reaction pattern by inspecting the parameter values and the square of the correlation coefficient.

• Journal of Korean Society of Environmental Engineers
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• v.33 no.2
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• pp.113-119
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• 2011

The study purpose was to examine an effect of zero emission of excess sludge on biological reactor and treated water quality within the biological reactor in the process of biological treatment combined with excess sludge reduction system with ozone. Under an ozone injection rate 0.03 g $O_3/g$ SS, Sludge Disintegration Number (SDN) 3 and less than pH 4 as pre-treatment process, it was possible to maintain a stable biological treatment process without sludge disintegration. In the test of $OUR_{max}$, of sludge, its value was hardly under the condition of ozone injection rate 0.03 g $O_3/g$ SS. There were almost no changes of MLVSS/MLSS within biological reactor followed by a solubilization of excess sludge. Accumulation of microorganism within biological reactor was also not observed. After solubilization of excess sludge, an increase for organic matter and SS concentrations of an effluent was not observed and T-N concentration was reduced by increasing nitrification and denitrification rate within biological reactor. Most of T-P was not removed by zero emission of excess sludge and was leaked by being included in effluents.

• Journal of Life Science
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• v.13 no.6
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• pp.970-975
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• 2003

Microbial trichloroethylene (TCE) degradation using trickling biofilter (TBF) is a cost-effective treatment method, in which monooxygenase (MO) fortuitously transforms TCE via cometabolism. Simple TBF, however, could not be stably operated for long-term treatment of TCE due to the contradictory characteristics of cometabolism. In this paper, microbial biocatalyst and biofilm reactor system, a two-stage continuous stirred tank reactor (CSTR)/TBF system using Burkholderia cepacia G4 and Methylosinus trichosporium OB3b, are evaluated for the long-term continuous treatment of TCE. The maximum TCE elimination capacities were in the range of 28 and 525 mg TCE/1$.$day. The reactor systems were stably operated for more than 3∼12 months.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.15 no.6
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• pp.89-96
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• 2001

This paper presents a decision making technique of reasonable $O_2$quantity needed to resolve organic matter via microbe in wastewater treatment. Decision making system of inferring reasonable $O_2$quantity consists of three parts. The first part is to compute reasonable $O_2$quantity with given process data. The second part is to find output features of processed wastewater using process model when $O_2$quantity is changed to a value inferred from decision making system. The third part is to show the results of decision making system. In order to verify performance of proposed decision making system computer simulation was done with process data gathered during 40 days. Simulation result shows that $O_2$quantity can be reduced over 10% under the condition of satisfying the specifications for processed wastewater.

• KSBB Journal
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• v.17 no.4
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• pp.321-325
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• 2002

Chiral epoxides are valuable intermediates for the asymmetric synthesis of enantiopure bioactive compounds. Microbial epoxide hydrolases (EHs) are newly discovered enzymes and versatile biocatalysts for the preparation of chiral epoxides by enantioselective hydrolysis of cheap and easily available racemic epoxide substrates. EHs are commercially potential biocatalysts due to their characteristics such as high enantioselectivity, cofactor-independent catalysis, and easy-to-Prepare catalysts. In this Paper, recent progresses in biochemistry and molecular biology of EH and developments of novel reaction systems are reviewed to evaluate the commercial feasibility of EH-catalyzed hydrolytic kinetic resolution for the production of chiral epoxides.

• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.324-325
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• 2001

총 암모니아성 질소(TAN)은고밀도 양식에서 한계요소로 작용하는 수질인자 중의 하나이다. 생물학적 암모니아 처리공정의 효율적인 설계를 위해서는 생물반응기의 암모니아 제거속도식을 구하여 처리시스템의 최적 용량을 구하여야 한다. 그러나 현재까지 진행된 고정화 미생물을 이용한 암모니아 제거공정에 대한 연구는 고정화 재질의 특성이나 장치의 운전효율에 대한 것으로 속도식에 대한 연구는 부족하다. (중략)

• Transactions of the Korean Society of Mechanical Engineers B
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• v.39 no.8
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• pp.633-643
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• 2015

In this study, we develop a highly efficient conical-air diffuser that generates fine bubble. By inserting a sufficient number of aerotropic microorganisms with dissolved oxygen from an air diffuser and minimizing the air-channel blockages within the air diffuser, we expect to improve the efficiency and durability of the decomposition process for organic waste. To upgrade the conventional air diffuser, we perform experiments and numerical analysis to develop a conical-type that generates fine bubble, and which is free from nozzle blockage. We complement the air-diffuser design by numerically analyzing the internal air-flow pattern within the diffuser. Then, by applying the diffuser to a mockup bioreactor, we experimentally and numerically study the bubble behavior observed in the diffuser and the 2-phase fluid flow in the bioreactor. The results obtained include statistics of the cord length and increased velocity, and we investigate the mechanisms of the fluid-flow characteristics including bubble clouds. Throughout the study, we systemize the design procedures for the design of efficient air diffusers, and we visualize the fluid-flow patterns caused by bubble generation within the mockup bioreactor. These results will provide a meaningful basis for further study as well as the detection of oxygen transfer and fluid-flow characteristics in real-scale bio-reactors using sets of air diffusers.