Journal of the Korea Institute of Military Science and Technology (한국군사과학기술학회지)

The Korea Institute of Military Science and Technology (한국군사과학기술학회)
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Decontamination of Bacterial Spores Using a Bio-reaction System

생물 반응 시스템을 활용한 세균 포자 제독 시험

  • Yeonghwan Seo (Defense Advanced Science and Technology Research Institute, Agency for Defense Development) ;
  • Hanwool Park (Defense Advanced Science and Technology Research Institute, Agency for Defense Development) ;
  • Hongsuk Lee (Defense Advanced Science and Technology Research Institute, Agency for Defense Development) ;
  • Seongjoo Kim (Defense Advanced Science and Technology Research Institute, Agency for Defense Development)
  • 서영환 (국방과학연구소 국방첨단기술연구원) ;
  • 박한울 (국방과학연구소 국방첨단기술연구원) ;
  • 이홍석 (국방과학연구소 국방첨단기술연구원) ;
  • 김성주 (국방과학연구소 국방첨단기술연구원)
  • Received : 2024.05.13
  • Accepted : 2024.08.10
  • Published : 2024.10.05
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Abstract

This report describes the test results of decontamination of Bacillus atrophaeus spores. The spore solution at 109 cfu/mL concentration was treated with chemical decontaminants, hydrogen peroxide, DF-200, sodium dichloroisocyanurate(NaDCC), and perasasfe. DF-200 was not suitable for decontamination of the spore solution. Among them, the optimal decontamination reaction conditions of perasafe and NaDCC were established from test tube experiments in a mL-scale, and they were validated in a bio-reaction system in a L-scale. The optimal conditions of perasafe and NaDCC were 1.0 % of concentration with 5 minutes of reaction time and 0.3 % of concentration with 10 minutes of reaction time, respectively. Also, both decontaminants required adequate agitation for decontamination. The results in the bio-reaction system were similar with the results from the smaller scale using test tubes, showing that the optimal conditions from the smaller scale experiments can be applied to the larger scale.

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References

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