• Proceedings of the Korean Society of Developmental Biology Conference
• /
• 1998.07a
• /
• pp.53-54
• /
• 1998

햄스터 난자에서 관찰되는 SAC의 활동은 30mmHg 이내의 음압자극에 대하여 잘 관찰되었으며 단일통로의 크기나 open time에 따라 4종류로 구분될 수 있었다. SAC 활동은 PKA에 의하여 활성이 조절되었으며 수정 후 할구분할이 진행될수록 SAC 전도도가 작은 통로로 전환되어 발생 초기 SAC 활도양상이 특징적인 변화가 관찰되었다.

• Proceedings of the Korea Water Resources Association Conference
• /
• 2021.06a
• /
• pp.445-445
• /
• 2021

대구시민의 삶의 질 향상에 크게 기여하는 신천은 홍수, 친수 및 하천환경 관리에 대한 필요성이 증가되는 상황에서 신천 복원을 위하여 하천횡단구조물 및 생태조사가 필요한 실정이다. 신천 내에 위치하고 있는 14개의 하천횡단구조물은 신천의 흐름을 가로막고 있어 수질악화 및 생물이동을 저해하고 있으며, 집중호우 등으로 인하여 구조물 주변에 대한 하천관리가 어려운 것이 현실이다. 특히 신천은 인구가 밀집해 있는 도심을 관통하는 하천으로 친수공간, 홍수 및 하천환경 관리에 대한 필요성이 증가하고 있다. 본 연구에서는 신천의 수질과 어류의 생태성의 상관관계를 확인하고 횡단구조물에 대한 해결방안을 제시하고자 한다. 현지조사 결과 총 3목 6과 10종의 어류가 관찰되었으며, 법정보호종인 멸종위기 야생생물 및 천연기념물은 관찰되지 않았으나, 고유종으로 긴몰개와 참갈겨니, 꺽지, 동사리 4종이 출현하였으며, 환경부 지정 생태계교란 생물 블루길 1종이 관찰되었다. 이러한 결과를 바탕으로 어류생물지수(FAI)를 검토하였을 때, 하류지역으로 갈수록 E등급으로 매우 나쁨의 환경상태를 나타내었다. 이는 상류에서 하류로 갈수록 비정상개체수가 증가하고 하상의 단순화 및 유기물의 축적으로 출현 종수의 감소에 따른 결과로 판단된다. 이러한 결과를 바탕으로 신천의 평균 FAI 지수는 14.71점으로 E등급의 매우 나쁨의 환경상태를 나타내는 것으로 분석되었다.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.16 no.4
• /
• pp.2971-2977
• /
• 2015

This study aimed to understand the changes in microbial community after algicide dosing to control the fish-killing dinoflagellate Cochlodinium polykrikoides in 10L microcosm. Based on our microcosm experiments, the algicidal activity for C. polykrikoides of yellow clay at the concentrations of 4g and 10g per 10 L was < 20%. At $0.8{\mu}M$ concentration of thiazolidinedione(TD49), the population of C. polykrikoides was controlled to be > 85%. In microbial community, a significant increase in heterotrophic bacterial (HB) abundance was observed at day 1 in the TD49 and yellow clay treatments including control. The HB remained high for 2 days and then gradually decreased. In contrast, the abundance of heterotrophic nanoflagellates (HNFs) increased significantly on days 3 and 5 in the TD49 treatments, indicating that the decline in HB was probably a result of predation by the high density of HNFs. In addition, fluctuations in the aloricate ciliate Uronema sp., which feed on bacteria, was clearly correlated with fluctuations in HB abundance, with a lag period of 1-3 days. Therefore, the short-term responses of the HNF and Uronema sp. may have been a result of the rapidly increasing of HB abundance, which is related to degradation of the dense C. polykrikoides bloom, particularly in the TD49 treatment. In addition, large aloricate ciliate Euplotes sp. was significantly increased after reproduction of HNFs and Uronema sp. Consequently, the algicide TD49 had positive effect on the microbial communities, which indicates that the microbial loop was temporarily enhanced in the microcosm by energy flow from HB through HNFs to ciliate.

• Proceedings of the Zoological Society Korea Conference
• /
• 1998.10b
• /
• pp.376.1-376
• /
• 1998

• Korean Journal of Microbiology
• /
• v.46 no.4
• /
• pp.346-351
• /
• 2010

The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with mannitol, which the area of clear zone was measured to approximately 7.64 $cm^2$. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041].

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.532-535
• /
• 2000

Optical microscope, SEM and fluorescent microscope were used for qualitative and morphological studies of the attachment bacteria on PE substratum under anaerobic condition. The observation of optical microscopic has demonstrated that the initial attachment of bacteria began in crevices of surface. In SEM photographs, shape and structure of biofilm could be observed, but bacteria species and methanogens was not identified. A large number of methanogenic bacteria were showed on the surface of PE substratum by fluorescence under 480nm of radiation. It was estimated that methanogenic bacteria was related to initial attachment of bacteria under anaerobic condition.

• Korean Journal of Plant Taxonomy
• /
• v.53 no.2
• /
• pp.126-147
• /
• 2023

The taxonomic identity of Elaeagnus ×maritima and E. ×submacrophylla (Elaeagnaceae) in Korea is unclear, yet they are presumed to be hybrid taxa based on their morphology. To determine their hybrid origins, a morphological analysis (field surveys and specimen examinations) and a molecular analysis involving two nuclear ribosomal DNA (nrDNA) regions (internal transcribed spacer and 5S non-transcribed spacer) and one chloroplast DNA (cpDNA) region (matK) were conducted. The morphological analysis revealed that E. ×maritima showed certain morphological similarities to E. glabra, whereas E. ×submacrophylla showed certain morphological similarities to E. pungens. However, the molecular analysis indicated that E. ×maritima exhibited additive species-specific sites of E. glabra and E. macrophylla in the nrDNA regions. Notably, E. ×submacrophylla showed various aspects, with some individuals exhibiting additive species-specific sites of E. pungens and E. macrophylla in the nrDNA and E. macrophylla sequences in the cpDNA regions, some individuals exhibiting E. macrophylla sequences in the nrDNA and E. pungens sequences in the cpDNA regions, and some individuals displaying E. macrophylla sequences in both the nrDNA and cpDNA regions, despite an intermediate morphology between E. pungens and E. macrophylla. These results indicate that these two species are of hybrid origin and frequently cross between parental and hybrid individuals.

• 한국생물공학회:학술대회논문집
• /
• 2000.11a
• /
• pp.549-552
• /
• 2000

A microorganism which can desulfurize dibenzothiophene (DBT) to 2-hydroxybiphenyl (2-HBP) was isolated from coal samples. The rate of DBT desulfurization was enhanced by the presence of yeast extract. In the case of DBT desulfurization by resting cells the rate and extent of 2-HBP production was enhanced with the addition of Tween 80.

• The Journal of the Korea Contents Association
• /
• v.13 no.10
• /
• pp.200-213
• /
• 2013

As an exploratory research, this paper studied the characteristics of visitor experience in the world first digital aquarium 'IQuarium' by the qualitative observation method. Between June and August of 2013, the first and second qualitative observations were made to gather the data of the behavioral and linguistic responses of visitors. The results of this study were as follows: First, visitors experienced the transformation of their identity and escapism by participating the activities. Second, they experienced the entertaining competition spirit against other visitors for fun. Third, they acted as story-makers by actively participating into the missions. Fourth, they had educational experiences by obtaining information about sea life and experienced the aesthetical atmosphere of 'IQuarium'. Although this study has limitations of the subjective perspective based on qualitative observation method, it will provide proper insight into advanced research in similar fields in the future.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.30 no.5
• /
• pp.937-942
• /
• 2001

The present study was conducted to identify the mechanism about the regulation of appetite by examining the expression patterns of vasoactive intestinal peptide in the hypothalamus of either fasted for 24 hours or anorexia mutant mouse. In order to investigate expression pattern of the vasoactive intestinal peptide, immunohisto-chemistry was employed along with reverse transcription polymerase chain reaction (RT-PCR) and dot blotting. Immunohistochemistry has shown that level of expression of vasoactive intestinal peptide and appetite-suppessing neuropeptide, was lower in the suprachiasmatic nucleus (SCN) and higher in the paraventricular nucleus (PVN) of the anorexia mutant group than in the comparable regions in the control group. This pattern was repeated in the fasting group, which also showed lower and higher levels of vasoactive intestinal peptide expression in the SCN and PVN respectively, In contrast, the vasoactive intestinal peptide mRNA level in the entire hypothalamus via RT-PCR and dot blotting was similar in the fasting and control groups, while it was significantly increased in the anorexia mutant group.