• The Korean Journal of Zoology
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• v.13 no.3
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• pp.85-93
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• 1970

Measurements were made of the $Ca^++$ uptake, oxygen consumption and ATPase activity of mitochondria extracted from the rat liver in sucrose-tris chloride medium. $Ca^++$ binding of mitochondria was not affected by the incubation temperature in the range of $0^\\circ - 37^\\circ C$. Succinate did not increase the amount of $Ca^++$ bound while it increased oxygen consumption highly. The presence of ATP in the incubation medium did not enhance the $Ca^++$ uptake either. Therefore, it is concluded that the initial binding of $Ca^++$ is independent on metabolism.

• Korean Journal of Poultry Science
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• v.15 no.2
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• pp.115-145
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• 1988

우리나라 축산업이 과학적인 축산으로 변모하는 과정에서 선도적 역할을 해온 것이 양계분야인 만큼 생산성적에 있어서도 어느 타 선진국에 뒤지지 않을 만큼 높아지고 있다. 그러나 사료원료의 해외 의존도가 높고 개방경제시대에 접어들음으로서 외국의 양계산물과 경쟁을 벌리게 되는 이 시점에서 생산비중 가장 높은 비율을 차지하는 사료를 가장 경제적으로 이용함으로서 생산 효율을 극대화시킬 책임이 우리에게 있다. 우리나라의 년 중 기온차이가 심하고 대부분의 산란계가 유창계사에서 사육되고 있는 만큼 계절에 따른 온도 변화에 맞추어 계절 사양을 함으로서 하절기에는 영양소 결핍현상을 방지하고 동절기에는 영양소 과다 섭취로 인한 영양소 허실을 줄일 수 있다. 난중에 따른 난가 형성이 되는 시장 상황에서 난중을 조절함으로서 생산효율을 높일 수 있다. 비록 난중이 생리적인 현상에 의해서 결정되지만 닭이 섭취하는 아미노산과 지방의 함량을 증감함으로써 어느 정도의 난중조절이 가능하다. 산란율과 난중에 영향을 미치지 않는 범위에서는 산란계의 체중이 작을수록 경제성이 있다. 가급적 육성기간중의 사료비를 줄이고 성계 체중을 줄이는 방법으로 육성기간중의 사료비를 줄이고 성계 체중을 줄이는 방법으로 육성기간중 단백질 점증법이 시험되고 있다. 이러한 육성체계를 보급화 하기에 앞서서 좀 더 많은 비교 시험이 필요하리라 본다. 사료 원료의 영양가를 정확하게 평가하는 것은 닭이 필요로 하는 영양소 요구량을 충족시키기 위한 필수조건이 된다. 단지 영양성분의 화학분석만으로 사료배합에 임하지 말고 생물가를 측정함으로서 닭이 섭취하는 영양소의 이용률이 함께 고려하여야 한다.

• Journal of Korean Society on Water Environment
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• v.29 no.5
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• pp.691-702
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• 2013

This review paper summarizes the theory, application, and potential drawbacks of diffusive gradient in thin film (DGT) probe which is a widely used in-situ passive sampling technique for monitoring inorganic contaminants in aquatic environments. The DGT probe employs a series of layers including a filter membrane, a diffusive hydrogel, and an ionic exchange resin gel in a plastic unit. The filter side is exposed to an aquatic environment after which dissolved inorganic contaminants, such as heavy metals and nuclides, diffuse through the hydrogel and are accumulated in the resin gel. After retrieval, the contaminants in the resin gel are extracted by strong acid or base and the concentrations are determined by analytical instruments. Then aqueous concentrations of the inorganic contaminants can be estimated from a mathematical equation. The DGT has also been used to monitor nutrients, such as ${PO_4}^{3-}$, in lakes, streams, and estuaries, which might be helpful in assessing eutrophic potential in aquatic environments. DGT is a robust in-situ passive sampling techniques for investigating bioavailability, toxicity, and speciation of inorganic contaminants in aquatic environments, and can be an effective monitoring tool for risk assessment.

• 한국해양학회지
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• v.28 no.2
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• pp.107-113
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• 1993

Interrelationship between bacterial and phytoplankton variables and associations of this relationship with phytoplankton community structure were studied from July, 1990 to October, 1991 in the estuarine system of Mankyung and dongjin Rivers where estuarine mixing is very active. During the study period only bacterial production and chlorophyll were always significantly correlated (r$^2$>0.36, p<0.02), suggesting that phytoplankton-biomass related process seemed to be related to bacterial production in the estuarine waters. However, bacterial production comprised only a small fraction (on average<2.6) of primary production throughout the study period, suggesting that those parameters were uncoupled. this might be partly due that phytoplankton, food particles for zooplankton, food particles for zooplankton, were mostly composed of large and/or chain-forming diatoms.

• The Korean Journal of Zoology
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• v.14 no.2
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• pp.43-56
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• 1971

The Ca uptake by the fragmented sarcoplasmic reticulum of the rabbit skeletal muscle was measured under various concentrations of K, Mg, Caffeine, procaine and quinine. The ATPase activity of this reticular membrane was measured under the same conditions simultaneously. The saturation of Ca uptake was almost completed within 1 minute. The Ca uptake was inhibited by high concentrations of K (above 50 mM) and Mg (above 1 mM)in the absence of ATP. When ATP is present, however, the Ca uptake did not reflect the concentration of K, while it increased greatly as the concentration of Mg was increased. Caffeine and procaine caused the inhibition of Ca uptake in the presence of ATP, but quinine did not. The ATPase activity of the membrane was little affected by the concentration of K, while it was enhanced in the presence of Mg. Caffeine, procaine and quinine did not influence the ATPase activity.

• The Korean Journal of Zoology
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• v.34 no.1
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• pp.110-122
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• 1991

기름종개과의 N. toni, L. costota, N. multifasciata 3종에 대한 형태분석, 핵형분석 및 전기영동법에 의한 유전자 분석을 실시 각 종의 지리적 변이 및 계통적 유연관계를 조사하였다. 형태 측정치에 대한 discriminant 분석결과 연 yoni 월악산집단, 1. costoto 거제도집단 그리고 환multifasc지물 산철집단이 같은 종내 타 집단들과 형태상 완전히 분리 되어졌다. 핵형분석에서 N. muftifosciota와 1. costoto는 2n: 50으로 염색체수는 조사된 전 집단이 변리없이 동일하였다. N to티 삼척과 진부집단은 2n: 50으로 동일하였으나 고성집단은 2n=50, In : 48인 염색체상이 한개체에서 동시에 발견되었고, 인형성부위의 위치 및 형태가 타 집단과 차이가 있었다. 전기영동을 실시하여 총 28개의 유전자를 검출, 분석하였다. 3종중 N. multifosciata 청도집단의 유전적 변이 (HDi 123, HG : .160)가 가장 높았고, N. toni 고성집단이 가장 적었다. ( HD= .017, HG= .015). N. yoni 고성집단과 강릉집단은 각 집단의 고유한 genetic marker를 다수 갖고 있으며 타집단들과 유전적 근연관계도 매우 먼 것으로 나타나 각기 독립된 별종으로 추정된다.

• The Korean Journal of Zoology
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• v.34 no.2
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• pp.142-147
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• 1991

Angiotensin I-converting enzyme (ACE) activity has been characterized in the rice eel, Monopterus albus. Peak activity of ACE in plasma from the rice eel was shown at around pH 10, which was more alkaline compared to that of mammals. Chloride requirements for the optimal ACE activity were different from species to spedes. ACE inhibitors, EDTA, teprotide (SQ 20, 881), and captopril (SQ 14, 225) showed dose-dependent inhibitions of ACE activity in plasma from the rice eel as well as mammals. ACE activity in the rice eel was increased by CoCI2, and the enzyme activity was more unstable at high temperature as compared to mammals The highest activity of ACE among the various tissues in the rice eel was found in the brain.

• The Korean Journal of Zoology
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• v.33 no.1
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• pp.119-125
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• 1990

A Potent inhibitor of trypsin and other various serine proteases including chymotrypsin, elastase, kallikrein, plasmin and subtilisin, has been purified to homogeneity from chick skeletal muscle by convendonal chromatographic procedures. The Inhibitor has an apparent molecular weight of 66, 000 dalton as determined by gel filtration. When the purified inhibitor was electrophoresed in the presence of sodium dodecyl sulfate, there appeared rwo protein bands having molecular weights of 66, 000 and 64, 000 dalton. The 64, 000 dalton protein seems to be the product of 66, 000 dalton protein by a lin'ited proteolysis during the purification procedure or in viuo. Thus, it seems to consist of a single polypeptide. The inhibitor appeared to be glycoprotein and have an isoelectric point of 7.4. It contains relatively large amount (8.33 mole%) of cysteine residues.

• The Korean Journal of Zoology
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• v.20 no.2
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• pp.101-107
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• 1977

The effects of sodium azide, cAMP, G-strophanthin and dicumarol on the ATP-ase activity and Ca uptake of the fragmented sarcoplasmic reticulum of skeletal muscle were studied and the effects were compared with respect to the enzymatic activity and Ca transport. Sodium azide (0.05 mM) and G-strophanthin (0.25mM) caused no inhibition on either ATPase activity or Ca uptake. cAMP($1\\times10^{-6}\\sim5\\times10^{-4}$) had no effect on ATPase activity while inhibited Ca uptake. Dicumarol (0.05 mM) did not inhibit ATPase activity but caused a decreased Ca uptake of heavier fraction (8,000-12,000xG) of the reticulum fragments.

• The Korean Journal of Zoology
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• v.2 no.1
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• pp.11-16
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• 1959

가물치(Ophice phalus argus)의 안구망막에 존재하는 시각물질을 spectrophotometry 한 결과 다음과 같다. (1) 암순응된 가물치 안구망막에서 2 % digitonin 으로 시각물질을 추출하여 광조사전의 흡광 spectrum을 측정한 결과 흡광 maximum 은 350$\mu\textrm{m}$, 420$\mu\textrm{m}$, 550$\mu\textrm{m}$, 590$\mu\textrm{m}$에 있으며 있었으며 광조사후의 흡광 spectrum과의 difference spectrum 의 흡광 maximum 은 390$\mu\textrm{m}$, 427 $\pm$2$\mu\textrm{m}$, 550$\mu\textrm{m}$, 595$\mu\textrm{m}$에 있었다. (2) 가물치 망막중의 시각물질인 390$\mu\textrm{m}$, 427$\mu\textrm{m}$, 550$\mu\textrm{m}$, 595$\mu\textrm{m}$의 삼원색광에 대한 감광도는 다음과같다. a. 390$\mu\textrm{m}$은 blue light > red light = green light > white light . b.427$\mu\textrm{m}$ 은 red light >blue light > white light > green light c. 550 $\mu\textrm{m}$과 595$\mu\textrm{m}$ 은 blue light > red light > green light > white light (3) 390 $\mu\textrm{m}$ 과 427$\mu\textrm{m}$에서 흡광 spectrum의 maximum을 가지는 시각물질은 그 존재를 예시하던 새로운 색소이다. (4) 가물치의 시각물질은 증류수에도 용출되나 2 % digitonin 수용액으로 추출하였을때보다 명확하지 못하였다. (5) 가물치는 담수어임에도 불구하고 거북류와 같이 visual voilet 는 발견할 수 없었다.