• Title/Summary/Keyword: 생물자원센터

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Collection and Exhibition of Useful Plant Resources in Myanmar (미얀마 유용식물자원 수집 및 전시 활용)

  • Ahn, TaiHyeon;Goh, YeoBin;Bae, JunKyu;Lee, JeongHo;Lee, KiCheol
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.47-47
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    • 2019
  • 미얀마 Indo-Burma 지역은 13,500종의 식물종이 분포하고 있는 생물다양성 핫스팟이다. 북부 산악지역에서 남부의 망그로브 지역에 이르는 다양한 특성의 생태계로 구성되어있다. 그러나, 풍부한 생물 다양성은 인간에 의해 인위적인 간섭, 개발, 모노크림 재배와 같은 지속적인 삼림 벌채로 위협 받고 있다. Dprovidedc와 Krupnick (2018년)은 123과 472종의 미얀마 약용식물을 학명, 영명, 미얀마 식물명 그리고 미얀마 현지에서 쓰이고 있는 약용식물의 활용 및 보존 상태를 종합적으로 정리했다. 이 중 약 13%가 보존 상태에 대한 평가를 받았으며 IUCN의 멸종 위기 종 목록(IUCN2017)에 등재되었다. 비록, 전국에 다양한 식물 및 생물 다양성이 분포되어 있지만, 미얀마는 여전히 지속 가능한 관리와 사용을 위한 인적자원과 시설이 부족하다. 국립수목원(KNA)은 2013년부터 미얀마 천연자원환경보전부(MINISTRY OF NATURAL RESOURCES AND ENVIRONMENTAL CONSERVATION, MONREC)와 미얀마 산림과학원(Forest Research Institute, FRI)과 함께 미얀마 보호지역 및 Saggaing 지역 내 식물 다양성 보존을 위한 공동 현장조사, 연구 및 출판 등의 협력사업을 진행하고 있다. 또한 국립수목원(KNA)는 약용으로 가치가 있는 미얀마 식물 생체를 수집, 현지외 보전 중이며 이중 65과 326종의 식물 생체를 국립수목원(KNA)에 있는 열대식물자원연구센터에 보존과 교육 목적으로 전시 중이다. 이 기증은 미얀마에서 식물생체를 국외로 반출한 첫 번째 사례이며 국립수목원(KNA)은 2008년 독일에서 열대식물을 기증받은 사례 다음으로 두 번째로 외국 정부의 공식적인 대량 식물 도입 사례이다. 도입한 식물 중 Santalum album L. 과 Amorphopallus paeoniifolius (Dennst.) Nicolson. 은 IUCN Red List of Threated Spiences (IUCN2017)에 취약(Vulnerable, VU), 관심필요(Least Concern, LC)로 분류되어 있다. 국립수목원 (KNA)는 2019년 6월 17일 개최된 "2019 아태지역 산림주간 및 28차 아태지역 산림위원회"에 참가하여 미얀마 도입식물 전시회를 개최 하였고, 국내 최초로 Amorphopallus paeoniifolius (Dennst.) Nicolson.의 Leaf Cycle을 타임랩스 촬영하여 국립수목원 웹진(10월 호) 과 YouTube에 연구성과를 홍보하고 있다. 또한 국립수목원 열대식물자원연구센터는 2,700여종의 미얀마, 라오스 등 무한한 가치를 지닌 열대식물의 수집 보전으로 기초 응용 연구기반을 구축하여 국민들에게 열대식물의 중요성과 잠재적인 자원가치를 알리는 역할을 하고 있다.

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Production of Transgenic Bovine Embryos Following Nuclear Transfer of Bovine Fetal Fibroblasts Transfected by Foreign Genes (외래유전자를 도입한 소 태아세포의 핵치환에 의한 형질전환 소 수정란 생산)

  • Kil, K.S.;Uhm, S.J.;Kim, E.H.;Chung, H.J.;Kim, T.;Park, H.;Lee, H.T.;Chung, K.S.
    • Korean Journal of Animal Reproduction
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    • v.24 no.4
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    • pp.429-437
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    • 2000
  • This study investigated the successful introduction of genes of erythropoietin (EPO) and enhanced green fluorescent protein (EGFP) in bovine embryos following nuclear transfer of bovine fetal fibroblasts (bFF), which were transfected by retrovirus vector system. Non-starved bFF were, transferred into perivitelline space of enucleated oocytes. The bFF-oocyte units were accomplished by cell to cell fusion and activated with calcium inophore and 6-dimethylaminopurine. Reconstructed embryos were co-cultured with bovine oviduct epithelial cells in CRlaa medium for 8 days. Out of 187 (EPO) and 210 (EGFP) bovine eggs reconstructed by nuclear transfer, 149 (EPO : 80.0%) and 158 (EGFP : 75.2%) embryos were cleaved, and among them 36 (EPO : 24.2%) and 35 (EGFP : 22.2%) embryos developed to the blastocyst stage. Of these blastocysts, 100% integration of EPO gene in 36 embryos was determined by PCR, and 100% expression of EGFP gene in 35 embryos was observed under the fluorescent microscope. This result indicates that bovine oocytes reconstructed by nuclear transfer of transfected bFF can successfully develop to the blastocyst stage. Furthermore, this novel procedure may be presumably an attractive method efficiently to produce the transgenic cattles.

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Spatio-Temporal Distribution of Euphausiids in Korean Waters in 2016 (2016년 한국 근해 난바다곤쟁이류의 시·공간적 분포)

  • Lee, Bo Ram;Park, Wongyu;Lee, Hae Won;Choi, Jung Hwa;Oh, Taeg Yun;Kim, Doo Nam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.54 no.4
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    • pp.456-466
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    • 2021
  • The distribution and abundance of euphausiids were investigated in Korean waters in 2016. Euphausiids were sampled with a Bongo net. A CTD (Sea Bird Electronics 9 plus) measured the water temperature and salinity while sampling. Mean water temperature ranged from 4.2-31.0℃. The highest temperatures occur in September and lowest temperatures in February. The mean water salinity ranged from 27.9-34.8 psu, with the highest salinities in March and lowest in September. Euphausiid species in group W consisted of four species. Among the euphausiid species, Euphausia pacifica was the dominant species with peak densities in September. The E. pacifica from group W was distributed in the bottom cold water during summer when a thermocline was formed. Five euphausiid species occurred in group S. E. pacifica and E. nana were the dominant species. In group S, E. nana was located in the warm and saline Tsushima Warm Current, a branch of the influential Kuroshio Current. Five euphausiid species occurred in group E. E. pacifica and T. longipes were the dominant species. In group E, E. pacifica and T. longipes were distributed in the deep and cold waters, these species prefer low water temperatures and perform vertical migration. The distribution of euphausiids in Korean waters were highly influenced by mass water characteristics, such as temperature and salinity.

Characterization of Agronomic Traits and Evaluation of Lignan Contents in Asian and African Sesame (Sesamum indicum L.) Germplasms (아시아 및 아프리카 원산 참깨(Sesame indicum L.) 유전자원의 농업형질과 리그난 함량 평가)

  • Sookyeong Lee;Jungsook Sung;Gi-An Lee;Eunae Yoo;So Jeong Hwang;Weilan Li;Tae-Jin Yang
    • Korean Journal of Plant Resources
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    • v.36 no.4
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    • pp.413-434
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    • 2023
  • Sesame (Sesamum indicum L.) is an ancient oilseed crop, which is usually cultivated for its seeds. Sesame breeding aims to achieve high seed yield and quality, along with resistance to biotic or abiotic stresses. It is estimated that sesame is originated from Asia or Africa continent. In this study, we characterized 10 agronomic traits and evaluated lignan contents in 165 sesame germplasm originated from Asia or Africa, to select high-yield or high-lignan content accessions. Sesame germplasm showed diverse phenotypes and highly variable lignan contents (sesamin: 0.5-12.6 mg/g, sesamolin: 0.1-3.5 mg/g, lignan: 1.1-16.1 mg/g). Based on originated continent, there are significant difference in agronomic traits, but no in lignan content. Correlation analysis revealed that yield-related agronomic traits were negatively related with lignan contents. Also, PCA analysis showed that most agronomic traits and lignan contents were principal components explaining diversity of whole sesame germplasm. Sesame germplasm was clustered into three groups based on agronomic traits and lignan contents. Finally, we selected high-yield (IT29416, IT167042, K276848, K276849) and high-lignan candidate accessions (IT169254, IT170031, IT169250, IT154876, IT170034), respectively. These accessions are expected to be valuable resources for breeding of high-yield and high-lignan contents functional cultivars.

Toxic effect of chlorothalonil, an antifouling agent, on survival and population growth rate of a marine rotifer, Brachionus plicatilis (해산 로티퍼(Brachionus plicatilis)의 생존율 및 개체군 성장률을 이용한 신방오물질(Chlorothalonil)의 독성영향)

  • Heo, Seung;Lee, Ju-Wook;Choi, Hoon;Yoon, Sung-Jin;Kwon, Ki-Young;Hwang, Un-Ki;Park, Yun-Ho
    • Korean Journal of Environmental Biology
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    • v.39 no.3
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    • pp.390-398
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    • 2021
  • In this study, the toxicity of chlorothalonil was investigated using survival rate and population growth rate of a marine rotifer, Brachionus plicatilis, typically used in live food in marine aquaculture systems. The survival rate of B. plicatilis was determined after 24 h of exposure to chlorothalonil (0.010-0.156 mg L-1). Population growth rate of B. plicatilis was calculated after 72 h of exposure to chlorothalonil (0.078-1.250 mg L-1). The survival rate and population growth rate of B. plicatilis exposed to chlorothalonil in single-dose toxicity assessment showed concentration-dependent reductions. Survival rates of B. plicatilis exposed to chlorothalonil had the following values: NOEC, 0.020mg L-1; LOEC, 0.039 mg L-1; and EC50, 0.057 mg L-1. Population growth rate of B. plicatilis exposed to chlorothalonil had the following values: NOEC, 0.156 mg L-1; LOEC, 0.313 mg L-1; and EC50, 0.506 mg L-1. When the residual concentration of chlorothalonil in the marine coastal area was more than 0.039 mg L-1, it had a toxic effect on B. plicatilis, a zooplankton. This paper provides toxicity values that can be used as baseline data for organizing environmental standards of chlorothalonil. It also provides insight into toxic effects of chlorothalonil on other non-target organisms.

Variations in Species Composition of Fishes in the Eelgrass Beds of Punghwa-ri, Tongyeong, Korea (경남 통영 풍화리 잘피밭의 어류 종조성 변동)

  • Jin-Sung Kim;Yeon-Hee No;Yong-Deuk Lee;Chan-Gyo Jeong;Woo-Seok Gwak
    • Korean Journal of Ichthyology
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    • v.35 no.2
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    • pp.101-112
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    • 2023
  • The survey was conducted to find out the temporal variation of the fish species in the eelgrass beds in Punghwa-ri, Tongyeong-si, Gyeongsangnam-do from April 2021 to March 2022. In comparison with the results of previous study conducted 10 years ago, there was a significant difference in species composition and dominant species. In addition, this study found that subtropical fish species such as Siganus fuscescens, Plotosus lineatus, Petroscirtes breviceps, which were not reported in the previous study.

Occurrence and Spatial Distribution of Marine Mammals by Sighting Surveys in Korean Waters During 2011-2020 (2011-2020년간 목시조사에 의한 우리나라 연근해 해양포유류 출현 및 분포 현황)

  • Lee, Jong Hee;Kim, Eun Ho;Lee, Kyunglee;Park, Kyum Joon;An, Yong-Rock;Kim, Hyun Woo;Sohn, Hawsun;Choi, Seok-Gwan
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.55 no.6
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    • pp.938-945
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    • 2022
  • National Institute of Fisheries Science conducted a total of 39 sighting surveys for marine mammals over 624 sighting days that covered 20,771 nautical miles in Korean waters during 2011-2020. Marine mammals were detected when 83.9% of the marine environment was less than or equal to three on the Beaufort wind scale. Thirteen species of marine mammals were identified, including two species of pinniped, with a majority of cetacean species. Frequently found species were narrow-ridged finless porpoise, followed by common dolphin, minke whale, Dall's porpoise, and Pacific white-sided dolphin. Narrow-ridged finless porpoises and minke whales were located in and offshore around the Korean peninsula, common and Pacific white-sided dolphins were exclusively found in the East Sea for most seasons. The other marine mammals were spotted in some seas and for limited durations.

The Strategy of Population Maintenance by Coastal Copepod Inferred from Seasonal Variations in Abundance of Adults and Resting Eggs (연안 요각류의 성체와 휴면란의 계절별 개체수 변화를 통한 개체군 유지 전략)

  • Park, Chailinn;Ju, Se-Jong;Park, Wongyu;Kim, Hyun-Woo;Lee, Soo Rin;Park, Jeong-Ho
    • Ocean and Polar Research
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    • v.40 no.4
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    • pp.213-222
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    • 2018
  • We investigated seasonal variations in the abundance of the adults and the resting eggs of copepods to understand the role of copepod resting eggs for maintaining their population inhabiting the coastal area of Dadaepo, Korea. Adults and resting eggs of copepods were collected bi-monthly with a conical net (45 cm mouth diameter, $330{\mu}m$ mesh size) and van Veen grab ($0.1m^2$ area), respectively, from October 2016 to September 2017. The species of resting eggs were identified using mtCOI gene. The mean abundance of copepods was highest in October ($3686{\pm}1190inds{\cdot}m^{-3}$) and lowest in January ($176{\pm}60inds{\cdot}m^{-3}$) with the dominance of Paracalanus parvus s.l.. Among copepod producing resting eggs, Acartia omorii and Centropages abdominalis were dominant. The mean abundance of resting eggs was the highest in July ($9148{\pm}6787eggs{\cdot}m^{-2}$) and the lowest in October ($530{\pm}348eggs{\cdot}m^{-2}$). Most of the collected resting eggs were identified as A. omorii's. The mean abundances of A. omorii adults and resting eggs were highest in July, and both abundances fluctuated in a similar pattern except in September. In September, A. omorii adults were observed in a state of low abundance, while their resting eggs occurred in a state of high abundance. These results suggest that A. omorii maintain their population by producing a large quantity of resting eggs, particularly diapause eggs, before the seawater temperature rises. These eggs would hatch and be newly recruited to their population when the environmental condition becomes favorable.

Genetic Population Structure of Euphausia pacifica in Korean Waters (한국 근해 태평양난바다곤쟁이(Euphausia pacifica)의 유전적 개체군 구조)

  • Bo Ram Lee;Wongyu Park;Hwan Sung Ji ;Hyo Jae Yu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.56 no.5
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    • pp.701-707
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    • 2023
  • We investigated Euphausia pacifica population in Korean waters in 2016 By samplings for genetic structur at five stations. Three sampling stations were located in the middle of the water masses which were clustered by temperature and salinity whereas the other stations were at the boundaries of the water masses. We amplified a 566 bp region and compared it with sequences of E. pacifica distributed in other waters. Sequences were classified two clades, and a clade was formed in the station E. Genetic distance of station E was close to E. pacifica present in Bering Sea, while it was distant to E. pacifica present in Yellow Sea near China. In genetic analysis, seven haplotypes were formed. Hap-1 and Hap-2 were shared in all five stations, while Hap-3 was shared in station W and WS. Four independent haplotypes were present in station E. Haplotype and nucleotide diversity were the highest in station E and the lowest in station S. The FST distances between station E and other stations were the highest, but distances among other stations were low. As a result, we concluded that E. pacifica, which is distributed in Korean waters, has a genetic population differentiation in the East Sea (station E).