• Journal of Life Science
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• v.16 no.7 s.80
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• pp.1235-1242
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• 2006

Sulforaphane, an isothiocyanate derived from hydrolysis of glucoraphanin in broccoli and other cruciferous vegetables, was shown to induce phase II detoxification enzymes and inhibit chemically induced mammary tumors in rodents. Recently, sulforaphane is known to induce cell cycle arrest and apoptosis in human canter cells, however its molecular mechanisms are poorly understood. In tile present study, we demonstrated that sulforaphane acted to inhibit proliferation and induce morphological changes of human hepatocarcinoma HepG2 cells. Treatment of HepG2 cells with $10{\mu}M\;or\;15{\mu}M$ sulforaphane resulted in significant G2/M cell cycle arrest as determined by DNA flow cytometry. Moreover, $20{\mu}M$ sulforaphane significantly induced the population of sub-G1 cells suggesting that sulforaphane induced apoptosis. This anti-proliferative effect of sulforaphane was accompanied by a marked inhibition of ryclin A, cyclin 31 and Cdc2 protein. However, the levels of tumor suppressor p53 and Cdk inhibitor p21 mRNA and protein expression were significantly increased by sulforaphane treatment in a concentration-dependent manner. Although further studies are needed, the present work suggests that sulforaphane may be a potential rhemoprevetiveichemotherapeucc agent for the treatment of human cancer cells.

• Journal of Life Science
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• v.15 no.4 s.71
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• pp.626-632
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• 2005

The polyamines are essential components of all eukaryotic cells and absolutely necessary for cell growth. In the present study, the cytoprotective role of polyamine was characterized. When $Ac_2F$ rat liver cells were treated with 1M 2,2'-azobis (2-amidinopropane) dehydrochloride (AAPH), a water soluble free radical initiator, viability of the cells was noticeably decreased due to the increase of reactive oxygen species (ROS). The cytotoxic effect of AAPH as well as ROS generation were significantly inhibited by the treatment of polyamines. Among polyamines, especially spermine at $20{\mu}M$ concentration exerted over $45\%$ inhibition of AAPH-induced ROS generation. Western blotting was performed to determine whether superoxide dismutase(SOD) or catalase (CAT) expression was involved in oxidative stress. The AAPH treatment blocked both SOD and CAT protein expressions. Spermine could recover those protein expressions to the untreated control levels. According to the result of cycline E measurement, AAPH might block the entry of the cells into S phase of the cell cycle. The reduced expression of cyclin E protein could be fully recovered by the addition of spermine. The antioxidative effects of spermine was also further proved by the apopotitic morphological analysis using ethidium bromide and acridine orange.

• Proceedings of the Korea Inteligent Information System Society Conference
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• 2000.11a
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• pp.397-406
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• 2000

대부분의 국방정보시스템의 소프트웨어는 높은 가용성, 신뢰성, 신속성, 정확성 등을 요구하는 대규모이면서 복잡한 실시간 시스템이다. 이러한 국방정보시스템의 소프트웨어 개발사업에 있어서 저비용 고효율의 미개국방경영 건설을 위하고 강한 전투력을 육성하기 위해서는 국방정보시스템의 효율적인 소프트웨어 개발사법이 요구된다. 따라서, 국방정보시스템의 소프트웨어 사업관리자가 개발사업을 관리하고 감독하는데 있어서 개발자와 사용자간의 조정 및 통제 기능을 수행하고 해당 국방정보시스템의 특성을 파악하여 성공적인 사업수행을 할 수 있도록 기술적인 사업관리 측면에서 구체적이고 상세화된 방안/지침을 제공하기 위한 전문가시스템의 지식베이스 도메인 지식개발에 관한 연구이다. 기존의 국방정보시스템의 사업관리자가 경험을 동해 축적해 온 기술, 정책, 아이디어, 노하우 등에 대한 지식을 습득하고 사업 관련자료에서 제시한 소프트웨어 생명주기 단계별 방안이나 지침 등을 바탕으로 하여 식별된 사실이나 내용을 지식베이스로 구축하여 국방정보시스템의 사업관리자가 필요로 할 때 설명모듈을 거쳐 임무 및 세부활동사항을 게시하여 줌으로써 사업관리 경험이 부족하거나 사업관리자가 교체되었을 때 사업관리자들이 업무를 지속적으로 연계시켜 임무수행이 가능하도록 기초/기반 여건을 제공하고자 한다. 본 논문은 국방정보시스템의 소프트웨어 개발사업에서 소프트웨어 생명주기 단계별 사업관리자의 임무 및 세부활동사항 지원용 전문가시스템을 개발할 때 이용할 수 있도록 도메인 지식을 개발하는 것이며 논문의 결과를 활용시 기대되는 효과는 본문을 참고 바란다.의 장점을 취합하여 설계되었다. 본 시스템은 기존의 UN/EDIFACT표준을 사용하고 있는 EDI환경과 기존 VAN 방식의 EDI 중계 시스템과 연동되며, 향후 관세청의 XML/EDI 표준 시행을 미리 대비하는 선도연구로서 자리매김이 된다. 본 연구에서는 개발된 XML/EDI 통관시스템은 향후, 서비스의 최대 걸림돌이 되어왔던 값비싼 EDI 사용료의 부담에서 벗어날 수 있게 할 것이며, 저렴한 EDI구축/운영 비용으로 전자문서교환의 활성화와 XML이 인터넷 기반의 문서유통 표준으로 자리매김할 수 있는 중요한 계기가 될 것이다.재무/비재무적 지표를 고려한 인공신경망기법의 예측적중률이 높은 것으로 나타났다. 즉, 로지스틱회귀 분석의 재무적 지표모형은 훈련, 시험용이 84.45%, 85.10%인 반면, 재무/비재무적 지표모형은 84.45%, 85.08%로서 거의 동일한 예측적중률을 가졌으나 인공신경망기법 분석에서는 재무적 지표모형이 92.23%, 85.10%인 반면, 재무/비재무적 지표모형에서는 91.12%, 88.06%로서 향상된 예측적중률을 나타내었다.ting LMS according to increasing the step-size parameter $\mu$ in the experimentally computed. learning curve. Also we find that convergence speed of proposed algorithm is increased by (B+1) time proportional to B which B is

• The KIPS Transactions:PartD
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• v.15D no.5
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• pp.705-712
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• 2008

Recently, interests in the notion of PKMS(Process based on Knowledge Management System) utilizing BPMS(Business Process Management System) and KMS(Knowledge Management System) have been significantly increased. Specially, most public organizations require their own effective knowledge management strategies since public business service needs various knowledge types. Based on a comprehensive framework that reflects lifecycle requirements of KMS and BPMS, we propose an PKMS system architecture, which performs step-by-step knowledge-providing strategy in public organization. To propose a PKMS architecture, this paper first investigates inter-relationships between public business and various knowledge types, and classifies knowledge types into three groups and then we suggest knowledge management strategies considering priority order among knowledge groups. Based on the proposed knowledge management, a PKMS system architecture can combine the advantages of the KM and BPM paradigms. This paper presents the PKMS system applied to employment insurance business part in real environment and demonstrated the advantages via inter-relationships between KM and BPM requirements.

• Journal of Life Science
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• v.28 no.12
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• pp.1469-1476
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• 2018

Occurrence of the Warburg effect in solid tumors causes resistance to cancer chemotherapy, and targeting energy metabolisms such as aerobic glycolysis is a potential strategy for alternative treatment. Dichloroacetate (DCA), an inhibitor of pyruvate dehydrogenase kinase (PDK), shifts glucose metabolism from aerobic glycolysis to oxidative phosphorylation (OxPhos) in many cancers. In this study, we investigated the anticancer effect of DCA on a human anaplastic thyroid cancer (ATC) cell line, 8505C. We found that DCA selectively inhibits cell proliferation of the 8505C line but not of a normal thyroid line. In 8505C, the cell cycle was arrested at the G1/S phase with DCA treatment as a result of decreased antiapoptotic proteins such as $HIF1{\alpha}$, PDK1, and Bcl-2 and increased proapoptotic proteins such as Bax and p21. DCA treatment enhanced the production of reactive oxygen species which consequently induced cell cycle arrest and apoptosis. Interestingly, DCA treatment not only reduced lactate production but also increased the expression of sodium-iodine symporter, indicating that it restores the OxPhos of glucose metabolism and the iodine metabolism of the ATC. Taken together, our findings suggest that PDK inhibitors such as DCA could be useful anticancer drugs for the treatment of ATC and may also be helpful in combination with chemotherapy and radiotherapy.

• Korean Journal of Clinical Laboratory Science
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• v.50 no.4
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• pp.457-461
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• 2018

Corpus luteum (CL) is a transient endocrinal tissue that undergoes repeated formation and regression during the estrous cycle. In this study, we hypothesized that the functional and structural mechanism of angiogenesis is similar between CL and tumor formation. First, we measured mRNA and protein expression of angiogenic receptors (vascular endothelial growth factor receptor-2, VEGFR2; Tie 2) in the early, middle, and late phase CL tissues during the estrous cycle. Ral-interacting protein of 76 kDa (RLIP76) and H-ras mRNA and protein were also expressed in the CL tissues. VEGFR2 and Tie 2 mRNA and protein were expressed in the early and middle phase CLs and decreased in the late phase. H-ras mRNA and protein were expressed in the early and middle phase CLs, but not in the late phase. RLIP76 mRNA was expressed in all phase CLs, and the protein expression was highest in early phase CLs. We suggest that RLIP76 and H-ras, an oncogenic gene, regulate the function of the CL during the estrous cycle, and the proteins will play an important role in the angiogenic mechanism of the CL.

• The Journal of The Korea Institute of Intelligent Transport Systems
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• v.22 no.3
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• pp.88-103
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• 2023

With the recent advancement of 4th Industrial Revolution technology, transportation systems are generating large amounts of mobility data related to the individual movement trajectories of vehicles and people. There are many constraints on utilizing mobility data containing personal information. Thus, in South Korea, the processing and generation of pseudonymized information and the analysis and utilization of this information have been managed in a dual manner by applying separate agencies and technologies through the revision of the Data 3 Act and the enactment of the Data Basic Act. However, this dual approach fails to securely support the entire data lifecycle and suffers from inefficiencies in terms of processing time and cost. Therefore, to compensate for the problems of the existing Expert Data Combination System and Data Safety Zone, this study proposes an Integrated Data Safety Zone Framework that integrates and unifies the process of generating, processing, analyzing, and utilizing mobility data. The integrated process for data processing was redesigned, and common requirements and core technologies were derived. The result is an architecture for a next-generation Integrated Data Safety Zone system that can manage and utilize the entire life cycle of mobility data at one stop.

• Journal of Life Science
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• v.19 no.9
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• pp.1314-1320
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• 2009

Histone deacetylase inhibitor (HDACI) is a new promising candidate as an antineoplastic agent for the treatment of solid and hematologic malignancies. In order to evaluate cell death and to elucidate the related mechanism(s) in NSCLC cells after HDACI, sodium butyrate (SB), a representative HDACI, was used to treat H460 cells for 48 hrs. SB exposure resulted in a significant reduction of cell viability at concentrations below 7.5 mM, and about 50% of cell death occurred at 20 mM. The types of cell death induced by SB were both apoptosis and necrosis, evaluated by Annexin-V staining combined with propidium iodide. SB treatment significantly evoked G2/M cell cycle arrest and subsequently induced cell death with caspase-dependent manner. While ERK protein content was not altered after SB, phosphorylated forms of ERK were markedly reduced. Taken together, SB is significantly able to induce cell death in NSCLC cell line H460, and it is suggested that the reduction of ERK phosphorylation might be closely involved in the cancer cell death mechanism initiated by HDACI.

• Journal of Life Science
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• v.20 no.3
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• pp.457-461
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• 2010

Familial hypokalemic periodic paralysis (HOKPP) is an autosomal dominant disorder characterized by reversible flaccid paralysis and intermittent hypokalemia. Although it has been reported that decreased activity in the $K_{ATP}$ channels of the skeletal muscle cell membrane plays a role in the pathogenesis of HOKPP, a clear mechanism has not yet been established. This study aimed to investigate the molecular biological mechanism underlying the decreased activity of $K_{ATP}$ channels in the skeletal muscles of familial HOKPP patients by studying the levels of the $K_{ATP}$ channel subunit Kir6.2. We found that when cells obtained from healthy individuals (normal cells) and HOKPP patients (patient cells) were treated with 4 mM potassium buffer, there was no quantitative change in the KCNJ11 mRNA levels and no difference in the Kir6.2 protein expression in the cytosol and cell membrane. On the other hand, when 1 mM potassium buffer was used, normal cells showed decreased expression of KCNJ11 mRNA as well as decreased expression of Kir6.2 protein in the cell membrane. However, patient cells treated with the same buffer showed no quantitative change in the levels of KCNJ11 mRNA or in the levels of Kir6.2 protein in the cytosol and cell membrane. Thus, in HOKPP patients, the Kir6.2 protein cannot be transported from the cell membrane to the cytosol, leading to closure of the $K_{ATP}$ channels, induction of depolarization, and subsequently, to the paralytic symptoms observed in the patient. Our findings thus provide new insights into the pathogenesis of HOKPP.

• Microbiology and Biotechnology Letters
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• v.42 no.1
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• pp.73-81
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• 2014

Salsola collina, also known as Russian thistle, is widely distributed in and around waste facilities, roadsides, and drought and semi-drought areas, and is used as a traditional folk remedy in Chinese medicine for the treatment of hypertension. In this study, we have evaluated the anti-oxidative and anti-cancer activities of the ethanol extract of S. collina Pall. (EESC), and the molecular mechanisms of its anti-cancer effects on human colon carcinoma HT29 cells. EESC exhibited anti-oxidative activity through DPPH radical scavenging capacity and showed cytotoxic activity in a dose-dependent manner in HT29 cells. After EESC treatment, HT29 cells altered their morphology, becoming smaller and irregular in shape. EESC also induced cell accumulation in the G2/M phase in a dose-dependent manner, accompanied by a decrease of cell population in the G1 phase. The G2/M arrest by EESC was associated with the increased expression of cyclin-dependent kinase (CDK) inhibitor p21 and Wee1 kinase, which phosphorylates, or inactivates, Cdc2. EESC treatment induced the phosphorylation of Cdc2 and Cdc25C, and inhibited cyclin A and Cdc25C protein expression. In addition, S arrest was induced by the highest concentration of EESC treatment, associated with a decrease of cyclin A and Cdk2 expression. These findings suggest that EESC may possess remarkable anti-oxidative activity and exert an anti-cancer effect in HT29 cells by cell cycle regulation.