• Title/Summary/Keyword: 생명주기

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Effect of Light on Production of Athocyanin and Betacyanin Thruough Cell Suspension Culture Systems in Vitis vinyfera L. and Phytolacca americana L. (포도와 미국자리공 세포현탁배양계 안토시아닌과 베타시아닌에 미치는 광의 영향)

  • 최관삼;인준교;이영복
    • Korean Journal of Plant Tissue Culture
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    • v.21 no.1
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    • pp.47-53
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    • 1994
  • The effects of light on the production of anthocyanin and betacyanin in cell suspension cultures of Vitis vinifera and Phytolacca americana were investigated. The cell growth of V.vinifera was little affected by exposure to light, but that of P.americana was markedly increased by light than in the dark In suspension cultures of V vinifera maximum accumulation of anthocyanin was observed during the stationary phase in continuous light By contrast, in suspension cultures of R americana, accumulation of betacyanin occured in parallel with cell division which showed two peaks after 4 days and 8 days of culture in continuous light whereas in continuous dark accumulation of anthocyanin and betacyanin did not occured However treatment of light interrupting for l, 12, and 24 h after 4 days in cell suspension. cultures of remarkably showed a slight anthocyanin accumulation, but after 8 days of culture remarkably accumulated by light interrupting for more than 12 h. In cultures of P. americana, the light treatment was more effective at 4th day than at 7th day after culture, but betacyanin accumulation was decreased again in the dark after light treatment These result indicate that the difference of light responses exist between the V.vinifera and the betacyanin of P. americana though cell suspension culture systems.

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Evaluation of soybean oil rancidity by pentanal and hexanal determination (Pentanal과 hexanal 측정에 의한 대두유의 산패도 측정)

  • Chun, Ho-Nam;Kim, Ze-Uook
    • Applied Biological Chemistry
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    • v.34 no.2
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    • pp.149-153
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    • 1991
  • Several commercial soybean oils were stored at $20^{\circ}C,\;40^{\circ}C$ and $60^{\circ}C$ with daily exposure of fluorescent light for 12 hours and evaluated their rancidity by headspace gas chromatographic analysis of pentanal and hexanal. The data of gas chromatographic analysis was compared with organoleptic flavor evaluation. For headspace gas chromatographic analysis, the volatile compounds were recovered by porous polymer trap and flushed into a fused silica capillary column at $250^{\circ}C$, The pentanal and hexanal separated were identified by gas chromatography and gas chromatography-mass spectrometric method. The results showed that the contents of pentanal and hexanal were linearly increased during storage for 100 days. A very simple linear relationship was found between organoleptic flavor scores and amounts of two volatile compounds with very high correlation coefficient. A similar linear relationship was also obtained for acid and peroxide value with sensory data. This results suggested the possible implication of pentanal and hexanal as an quality index for rancidity evaluation of soybean oil.

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Proteomic Changes in Odae Polished White Rice Grown at Different Cultivation Conditions (재배환경에 따라 변화하는 오대벼 백미의 단백질체 분석)

  • Lee, Ju-Young;Lee, Jin-Woo;Kim, Young-Ran;Yeom, Yu-Jin;Lim, Jin-Kyu
    • Journal of Applied Biological Chemistry
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    • v.55 no.2
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    • pp.79-83
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    • 2012
  • It has been known that the proteome profiles in the period of growth and development of rice are changed by the growth conditions including temperature, soil, and fertilization. In this study, the proteome profiles of Odae polished white rice grown in Chulwon and Chilgog were compared on 2-dimensional(D) gels. The differentially expressed proteins were selected from the 112 identified total proteins and classified into functional groups. The most significantly differentially expressed proteins were stress responsive proteins; Ent-kaur-16-ene synthase, which is responsible for synthesizing a plant hormone gibberellin, was expressed in Chulwon rice and heat shock proteins were in Chilgog rice, respectively. Xylanase inhibitor protein, which inhibits the enzyme xylanase produced by pathogenic fungi and Bacilli, was expressed significantly high in Chilgog rice grown at high temperature. Differential expressions of transporter proteins were observed both in Chulwon and Chilgog rice. Regarding the facts that Chilgog rice contained relatively higher amount of proteins than Chulwon rice and Chulwon rice showed large number of proteins were differentially expressed, it can be concluded that different cultivation conditions could change the protein expression profiles in rice in various ways, including elevation of protein amount or differential expressions of specific proteins, etc. The results suggest that the characteristics of the profiles of the proteome in the polished white rice are definitely changed by the environmental factors including high temperature. The results can be utilized for the development of the proper cultivation conditions for the production of high quality rice with good palatability.

Virus-Resistance Analysis in Transgenic Tobacco Expressing Coat Protein Gene of Cucumber Mosaic Virus (오이모자이크바이러스 외피단백질유전자 발현 담배의 바이러스 저항성 분석)

  • 손성한;김경환;박종석;황덕주;한장호;이광웅;황영수
    • Korean Journal of Plant Tissue Culture
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    • v.24 no.3
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    • pp.153-160
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    • 1997
  • Cucumber mosaic virus (CMV) leads to a cause of poor crop productivity and quality. To solve this problem, we attempted to develop a virus-resistance tobacco plants by using viral coat protein (CP) gene. Transgenic tobacco plants expressing CMV CP gene were analysed by the resistance upon CMV infection. The virus-resistance was measured in $\textrm{T}_{1}$, generation by the inhibition of plant growth and the expression of the mosaic symptoms infected with CMV. The transgenic lines were divided into four groups: highly resistant, resistant, moderate and susceptible based on their growth and symptom severity. Out of 39 transgenic lines, 16 lines showed significant virus-resistance. And of resistant lines, 2 lines were designated highly resistant based on the facts that they achieved similar plant height to that of non-infected tobacco plants and showed lower disease symptom than that of other lines. The steady state level of CP RNA and coat protein level were measured by northern blot and immunoblot analysis. The CP RNA was highly accumulated in most resistant and moderate lines but barely detected in susceptible lines. The coat protein was detected in most lines regardless of their resistance to CMV. from this result, virus-resistance appeared to correlate more with CP RNA level than the level of coat protein. However, in two highly resistant lines, CP RNA level was unexpectedly low. This unexpected phenomenon need to be further investigated.

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Study on Establishment Criteria of Dam Emergency Action Plan (댐 비상대처계획(EAP) 수립기준 연구)

  • Park, Ki-Chan;Choi, Kyung-Sook
    • Proceedings of the Korea Water Resources Association Conference
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    • 2017.05a
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    • pp.257-257
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    • 2017
  • 본 연구는 기상이변의 영향으로 지속적으로 발생되고 있는 댐 저수지 붕괴에 대한 원인 분석과 기존의 관련 연구 및 지침 등을 비교 분석하여 현재 실무에서 적용되고 있는 댐 저수지 비상대처계획(EAP) 수립 대상 기준에서 제외되는 시설물의 붕괴 가능성 평가를 통해 EAP 수립 대상의 적정 범위를 제시하고자 하였다. 이를 위해 본 연구에서는 시설물의 붕괴 가능성에 대한 정량적 판단 기준을 각 요인별로 제시하고, EAP 수립 대상에서 제외되는 댐 저수지를 대상으로 붕괴 가능요인을 적용하여 3개 이하의 붕괴요인에 해당될 경우 저위험군, 4개 및 5개의 붕괴요인에 해당될 경우 중위험군과 고위험군으로 각각 분류하여 시설물 붕괴 위험성 정도에 따른 적절한 대응이 이루어질 수 있도록 제안하였다. 댐 저수지 붕괴 사례 분석을 통해 도출된 붕괴 가능성의 주요 요인으로는 저수용량, 제체 높이, 준공 경과연수, 우심피해 및 지진피해 잠재지역이 포함되며, 각 요인별 객관적인 판단기준은 댐 저수용량의 경우 EAP 법적 수립기준 및 과거 붕괴사례 등의 분석을 토대로 붕괴사례 61개소 중에서 약 88%를 차지하는 저수용량 10만톤을 선정하였으며, 댐 높이의 경우 댐의 파괴양상(이종태, 1987)에서 제시한 댐 붕괴부 특성과 미국주댐안전협회(ASDSO: American Society of Dam Safety Official) 학술지인 Dam Safety(2006) 등에서 제안한 기준을 기존의 댐?저수지 붕괴사례에 적용하여 댐 붕괴폭과 댐 높이의 상관관계 분석 결과를 바탕으로 댐 높이 8m 이상을 적정 범위로 고려하였다. 준공 경과연수의 경우에는 국민안전처 "재해위험저수지 댐 관리 지침" 타당성 평가기준인 '시설노후도' 기준과 한국시설안전공단 사회간접자본 노후화 기준에 해당하는 경과연수 기준을 토대로 30년 이상을 기준으로 선정하였으며, 우심피해 및 지진피해 잠재지역은 국민안전처 재해연보(2014)를 기준으로 과거 10년 동안의 우심피해 발생 지역과 기상청 국내 지진 규모 10위권 내에 포함되는 지역을 고려 대상으로 하였다. 본 연구에서 도출한 시설물 붕괴 가능성에 대한 판단기준을 국민안전처에서 고시한 EAP 수립 대상에서 제외되는 지자체 재해위험저수지 354개소에 적용해 본 결과, 저위험군 16개소, 중위험군 5개소, 고위험군 2개소로 각각 조사되었다. 저위험군은 전문 시설물 관리자에 의한 주기적인 시설물 점검 및 관찰이 필요할 것으로 사료되며, 중 고위험군에 속하는 시설물의 경우 본 연구의 결과를 바탕으로 시설물 하류지역의 실질적인 인명 및 경제적 피해 가능성에 대한 보다 면밀한 조사를 통해 EAP 수립 대상 포함여부를 결정하여야 할 것으로 판단된다.

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Predicting Program Code Changes Using a CNN Model (CNN 모델을 이용한 프로그램 코드 변경 예측)

  • Kim, Dong Kwan
    • Journal of the Korea Convergence Society
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    • v.12 no.9
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    • pp.11-19
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    • 2021
  • A software system is required to change during its life cycle due to various requirements such as adding functionalities, fixing bugs, and adjusting to new computing environments. Such program code modification should be considered as carefully as a new system development becase unexpected software errors could be introduced. In addition, when reusing open source programs, we can expect higher quality software if code changes of the open source program are predicted in advance. This paper proposes a Convolutional Neural Network (CNN)-based deep learning model to predict source code changes. In this paper, the prediction of code changes is considered as a kind of a binary classification problem in deep learning and labeled datasets are used for supervised learning. Java projects and code change logs are collected from GitHub for training and testing datasets. Software metrics are computed from the collected Java source code and they are used as input data for the proposed model to detect code changes. The performance of the proposed model has been measured by using evaluation metrics such as precision, recall, F1-score, and accuracy. The experimental results show the proposed CNN model has achieved 95% in terms of F1-Score and outperformed the multilayer percept-based DNN model whose F1-Score is 92%.

Spatial Pattern Analysis for Distribution of Migratory Insect Pests at Paddy Field in Jeolla-province (전라도 지역 논벼에서 비래해충 개체군 분포의 공간패턴분석)

  • Park, Taechul;Choe, Hojeong;Jeong, Hyoujin;Jang, Hojung;Kim, Kwang Ho;Park, Jung-Joon
    • Korean journal of applied entomology
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    • v.57 no.4
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    • pp.361-372
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    • 2018
  • Migratory insect pest populations migrate from the southern China to Korea through jet streams. In Korea, 5 major migratory insect species are important, i.e. Nilaparvata lugens, Sogatella furcifera, Laodelphax striatellus, Cnaphalocrocis medinalis and Mythimma separate, which are damages to the major crops, rice. This study was conducted from late July 2016 to early September 2016 and from July 2017 to August 2017 in rice paddy of Jeolla-province. C. medinalis and M. separata collected using pheromone traps, while N. lugens, S. furcifera and L. striatellus collected using 3 methods (visual surveys, sweeping surveys, sticky traps). SADIE (Spatial Analysis by Distance IndicEs) among geostatistics was used to analyze migratory insect pests. SADIE was used to analyze spatial distribution and index of aggregation $I_a$, index of clustering $V_i$, $V_j$ were used to investigate the spatial distribution. Also, the clustering indices were mapped as red-blue plot. C. medinalis and M. separata showed different distribution based on SADIE spatial aggregation analysis and red-blue plot analysis. Initial spatial distributions of L. striatellus and other planthoppers were differed for sampling location and time.

Changes in the Reproductive Population Size of the Huanren Brown Frog (Rana huanrenensis) and Wonsan Salamander (Hynobius leechii), which Breeding in Mountain Valleys, According to Climate Change (기후변화에 따른 산간계곡에 번식하는 계곡산개구리 (Rana huanrenensis)와 도롱뇽 (Hynobius leechii) 번식개체군 크기의 변동)

  • Choi, Woo-Jin;Park, Daesik;Kim, Ja-Kyeong;Lee, Jung-Hyun;Kim, Dae-In;Kim, Il-Hun
    • Korean Journal of Environment and Ecology
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    • v.32 no.6
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    • pp.582-590
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    • 2018
  • Although there are many studies of the effect of climate change on the breeding phenology and community diversity of amphibians, the studies of variations in reproductive population size of individual species according to climate change are still lacking. We examined the effect of climate change on the reproductive population size of Rana huanrenensis and Hynobius leechii, which bred in mountain valleys, by surveying the reproductive population of the two species between 2005 and 2012 and analyzing the correlation between the variation of the outdoor population and the surrounding climate change factors, obtained from a meteorological observatory located at 5.6 km from the study site. The size of the reproductive population of the two species commonly fluctuated with aan pproximately 3.5-year cycle. That of H. leechii, in particular, decreased significantly over eight years. The air temperature tended to more closely relate with the reproductive population size of R. huanrenensis as was the case of the precipitation with that of H. leechii. The yearly mean highest temperature and spring mean temperature variation consistently decreased over the eight years, and the latter was related with the significantly decreased size of H. leechii reproductive population. These results showed that recent climate change directly could affect the reproductive population size of amphibians, particularly H. leechii, which breeds in mountain valleys.

A Study on the Environmental Effects of Improvement of Activated Carbon Adsorption Tower for the Application of Activated Carbon Co-Regenerated System in Sihwa/Banwal Industrial Complex (시화반월산업단지 활성탄 공동재생시스템 적용을 위한 활성탄 흡착탑 개선에 따른 환경적 효과분석)

  • Choi, Ye Jin;Rhee, Young Woo;Chung, Gu Hoi;Kim, Duk Hyun;Park, Seung Joon
    • Clean Technology
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    • v.27 no.2
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    • pp.160-167
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    • 2021
  • This study investigated the environmental effects of improving the general-type activated carbon adsorption tower used at the Sihwa/Banwol Industrial Complex with use of a cartridge-type activated carbon adsorption tower for the application of an activated carbon co-regenerated system. Four general-type activated carbon adsorption towers and two cartridge-type activated carbon adsorption towers were selected to analyze the properties of activated carbon and to compare the efficiency of reducing environmental pollutants. The results showed that the activated carbon used in the cartridge-type activated carbon adsorption towers was high quality activated carbon with an iodine adsorption force of more than 800 mg/g and that a good adsorption performance was maintained within the replacement cycle. From an analysis of the environmental pollutant reduction efficiency, it was confirmed that the cartridge-type activated carbon adsorption tower functioned properly as a prevention facility for handling emissions pollutants with a treatment efficiency of total hydrocarbons (THC), toluene, and methylethylketone (MEK) components of 71%, 77%, and 80%, respectively. The general activated carbon adsorption tower, which was confirmed to use low-performance activated carbon, had a very low treatment efficiency and did not function properly as a prevention facility for dealing with emission pollutants. It is believed that it is possible to reduce pollutants during operations by changing from the general-type activated carbon adsorption tower to a cartridge-type activated carbon adsorption tower.

Increased Antioxidants of Agastache rugosa by the Night Interruption Time (야파(night interruption)처리에 의한 배초향의 항산화 물질 증가)

  • Kim, Sungjin;Noh, Seungwon;Park, Jongseok
    • Journal of Bio-Environment Control
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    • v.31 no.4
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    • pp.319-324
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    • 2022
  • The objective of this study was to determine the proper night interruption of photoperiods and dark periods for accumulating bioactive compounds of Agastache rugosa without decreasing plant growth. Five-week-old seedlings were transplanted in a DFT system with white LEDs. A. rugosa was treated with night interruption time treatments of 18:1:2:3, 18:2:2:2, 18:3:2:1 (light:dark:light:dark), and 20:4 (control) for 4 weeks. There were no significant differences except for leaf length, leaf width, and the number of flowers. The content of antioxidants in the shoot of A. rugosa was high in tilianin and acacetin, and the content of rosmarinic acid (RA) was significantly higher in the underground part. The RA content per dry weight of A. rugosa was 47.92 and 51.46% higher than that of the control in 18:1:2:3 and 18:2:2:2, and tilianin and acactin per dry weight were significantly higher in 18:3:2:1. There was no significant difference in growth due to the same day light integral, but 18:2:2:2 showed high total polyphenol contents. Therefore, it is thought that the effect of increasing secondary metabolites of A. rugosa without degradation of growth can be expected through night interruption treatment in plant factory cultivation systems using artificial light.