• Food Science of Animal Resources
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• v.30 no.3
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• pp.410-418
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• 2010

Staphylococcus aureus is one of the major pathogens that can cause staphylococcal infection and food poisoning. In this study, we compared conventional culture methods and real-time PCR for detection of S. aureus in artificially inoculated milk, sausage, raw pork, and vegetable salad. The performance of a coagulase test for confirming S. aureus was also compared with a colony PCR test. Bulk food samples (500 g each) were artificially inoculated with S. aureus and divided into 20 samples (25 g or mL each). All samples were added to tryptic soy broth (225 mL/sample) with 10% NaCl and incubated at $37^{\circ}C$ for 24 h. After the enrichment, broth cultures were streaked onto Baird-Parker (BP) agar with egg yolk tellulite, and incubated at $37^{\circ}C$ for 24 h. In addition, 1 mL of broth cultures was collected to perform real-time PCR. Two suspicious colonies from the BP agar were picked up and plated on nutrient agar and incubated at $37^{\circ}C$ for 24 h followed, by a coagulase confirmation test and a colony PCR analysis. There were no statistical differences between culture methods and realtime PCR in food samples with low background microflora, such as milk and sausage. However, a significant statistical difference was found between the culture methods and real-time PCR for raw pork and vegetable salad. Furthermore, the colony PCR test of the presumptive colonies on BP agar for confirming S. aureus is more accurate and efficient than the coagulase test for unprocessed foods.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.41 no.4
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• pp.361-373
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• 2015

This study was carried out to evaluate the antioxidant and antibacterial activities of Glycyrriza uralensis Fisher (Jecheon, Korea) extracts obtained by various extraction conditions (85% ethanol, heating temperatures and times), and to establish the optimal extraction condition of G. uralensis for the application as cosmetic ingredients. The extracts obtained under different conditions were concentrated and made in the powdered (sample-1) and were the crude extract solutions without concentration (sample-2). The antioxidant effects were determined by free radical scavenging activity ($FSC_{50}$), ROS scavenging activity ($OSC_{50}$), and cellular protective effects. Antibacterial activity was determined by minimum inhibitory concentration (MIC) on human skin flora. DPPH free radical scavenging activity of sample-1 ($100{\mu}g/mL$) was 10% higher in group extracted for 6 h than 12 h, but sample-2 didn't show any significant differences. The extraction yield extracted with same temperature for 12 h was 2.6 times higher than 6 h, but total flavonoid content was 1.1 times higher. These results indicated that total flavonoid content hardly increased with increasing extraction time. Free radical scavenging activity, ROS scavenging activity and cellular protective effects were not dependent on the yield of extraction, but total flavonoid content of extraction. Antibacterial activity on three skin flora (S. aureus, B. subtilis, P. acnes)of sample-1 in different extraction conditions were evaluated on same concentration, and the group extracted at 25 and $40^{\circ}C$ showed 16 times higher than methyl paraben ($2,500{\mu}g/mL$). In conclusion, 85% ethanol extracts of G. uralensis extracted at $40^{\circ}C$ for 6 h showed the highest antioxidant and antibacterial activity. These results indicate that the extraction condition is important to be optimized by comprehensive evaluation of extraction yield with various conditions, yield of active component, and activity test with concentrations, and activity of 100% extract, for manufacturing process of products.

• Journal of the Korean GEO-environmental Society
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• v.13 no.11
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• pp.81-91
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• 2012

This paper mainly deals with the analysis of probabilistic characteristics of the disturbed function proposed to predict dynamic behavior of Geosynthetic-soil interface as the lining and cover systems used in waste landfills. Calibration and statistical property estimation of the parameters in the disturbed function model were first performed using many experimental data obtained from a new multi-purpose interface apparatus (M-PIA). In order to analyze the effect due to changes in chemical degradation and normal loads condition, probabilistic properties such as mean, coefficient of variation and distribution type of the disturbed function were evaluated using both the LHS method known to be a very efficient sampling scheme and the estimated statistical property of A and Z. As a result, variation of the disturbed function is found to range approximately from 10~28% according to the level of ${\xi}_D$ and Weibull appears to be the most adequate distribution type at almost all levels of ${\xi}_D$. It is concluded that a probabilistic safety assessment method for Geosynthetic-soil interface considering uncertainty in shear strength can be developed by utilizing probabilistic properties of the disturbed function obtained in this study.

• Journal of the Korean Geotechnical Society
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• v.30 no.2
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• pp.53-64
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• 2014

This study sets it's face to define effects of the various loading periods in normal consolidation area on clay's compression and long-term consolidation characteristics through a laboratory test using homogeneous remolded clay. Moreover, by carrying out a long-term consolidation test which diversifies initial consolidation applicable to effective overburden loading in the various loading period. This study intended to suggest the method predicting the final settlement on the basis of loading periods by comparing and analyzing compression curve's characteristics according to loading weight of each stage and increase in loading period when carrying out the standard consolidation test. From the test results, the study shows that as of the soft clay's compression characteristics on the basis of various loading periods, preconsolidation load has a tendency to be decreased slightly as the loading period is getting more and more longer at each step after initial consolidation load puts on the remolded clay which is caused by secondary consolidation's increase in the latter part of each phase. And those effects have an weaker influence on compression index in normal consolidation area at the same time as secondary consolidation brought out quasi-overconsolidation and stabilization of clay's structure, have an influence re-compression index is increased in overconsolidation area on the other hand.

• Journal of the Korean GEO-environmental Society
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• v.5 no.2
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• pp.51-62
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• 2004

Granular compaction piles have the load bearing capacity of the soft ground increase and have the settlement of foundation built on the reinforced soil reduce. The granular compaction group piles also have the consolidation of the soft ground accelerate and have the liquefaction caused by earthquake prevent using the granular materials such as sand, gravel, stone etc. However, this method is one of unuseful methods in Korea. The Granular compaction piles are constructed by grouping it with a raft system. The confining pressure at the center of bulging failure depth is a major variable in relation to estimate for the ultimate bearing capacity of the granular compaction piles. Therefore, a share of loading is determined considering the effect of load concentration ratio between the granular compaction piles and surrounding soils, and varies the magnitude of the confining pressure. In this study, method for the determination of the ultimate bearing capacity is proposed to apply a change of the horizontal pressure considering bulging failure depth, surcharge and loaded area. Also, the ultimate bearing capacity of the granular compaction piles is evaluated on the basis of previous study on the estimation of the ultimate bearing capacity and compared with the results obtained from laboratory scale model tests. And using the result from laboratory model tests, it is studied increase effect of the bearing capacity on the granular compaction piles and variance of coefficient of consolidation for the ground.

• Journal of Food Hygiene and Safety
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• v.33 no.4
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• pp.306-309
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• 2018

The present study was conducted to investigate the prevalence of Mycoplasma spp. in cows and pigs with pneumonic lung lesions in Gyeonggi province in 2017. One hundred ninety two and 257 lung tissues were collected from slaughtered cows and pigs with pneumonic lesions, respectively, and examined for the presence of Mycoplasma spp. by a genus specific PCR. Among the examined animals, 147 cows (76.5%) and 203 pigs (80.9%) were found to be infected with Mycoplasma spp.. The infected tissues were further examined to identify the specific species of Mycoplasma using species specific PCRs. The only identified species in cows was M. agalactiae which was detected from 16 cows (8.3%), whereas M. dispar, M. bovis, and M. bovirhinis were not detected. In pigs, M. hyopneumoniae was detected from 74 pigs (28.8%) and M. hyorhinis from 13 pigs (5.1%). M. hyosynoviae was not detected. Taken together, the current study indicates Mycoplasma spp. are commonly associated with lung infection in cows and pigs in Korea. Further studies are needed to evaluate the impact of mycoplasma infection on the development of lung diseases in farm animals.

• The Korean Journal of Ecology
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• v.28 no.4
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• pp.223-230
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• 2005

The vegetation of Bijin and Yongcho Islets was investigated from February 1, 2000 to March 30, 2002, and forest structure was constructed. Vascular plants of this area consisted of 12 forms, 59 varieties, 476 species, 352 genera, 112 families and 37 orders. Among them, 37 species (6.8% out of the total 547 taxa) of evergreen broad leaved trees, 36 species (6.6% out of the total 547 taxa and 19.7% out of the total naturalized plants in Korea) of naturalized plants, 6 species of endemic plants and 41 species of cultivated plants were observed. Rare and endangered plants include 1 taxa: Crypsinus hastatus ('98-4). The vegetation in this study area was classified into 9 communities (Pinus thunbergii, Castanopsis cuspidata var thunbergii Camellia japonica, Neolitsea sericea, Machilus thunbergii, Quercus variabilis, Carpinus coreana, Platycarya strobilacea, Miscanthus sinensis var. purpurascens) and 1 afforestation (Alnus firma).

• The Journal of Engineering Geology
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• v.11 no.1
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• pp.1-11
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• 2001

The geological characteristics of Korea are that we can encounter the rock layer only after 10m of excavation, methods to presume the rock pressure distribution of the rock layer is urgently needed. When using the existing empiric science of Terzaghi-Peck, Tschebotarioff to measure the rock pressure of the rock layer, underestimate the real strength because of the cohesion is ignored. Therefore calculating the horizontal sliding force of wedge block, which includes the dips and shear strength of discontinuities and surcharge load etc., think to be to getting a closer rock stress of the real rock pressure acting upon the earth structure in rock mass. This research use Coulomb soil pressure theory assuming that the backfill soil will yield wedge failure when it has cohesion, applying Prakash-Saran(l963), and then it uses equilibrium of force and shear strength $\tau$=c+$\sigma$tan $\Phi$ of the cliscontinuities. Analyzing shear strength and dips of cliscontinuities using calculated theory according to the status of discontinuities aperture, we were able to find out that because the cohesion and friction angle of the rock layer itself is large enough, how the dip directions and dips facing the excavation face is the only factor deciding whether or not the rock stress is applied. The evaluated theory of this research should be strictly estimated, so that the many parameters such as c, $\Phi$value, types and structures of rock class, excessive lateral pressure, dynamic load, earthquake, needed later when calculating shear strength of discontinuities and especially the ground water effect acting on rock layer should be coumpted with many measuring data achieve at the insite to study the application.

• Korean Journal of Agricultural and Forest Meteorology
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• v.7 no.3
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• pp.211-219
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• 2005

Vegetation composition and structure of Sorbus commixta - native forests of South Korea were studied using phytosociological procedures and its ecological characteristics analyzed with special respect to species correlations, importance values, and constancy classes. Vegetation types were divided (great division) into Tripterygium regelii - Quercus mongolica (Inland high mountain type) and Acer takesimense - Fague crenata var. multinervis (Ulleung Island type) and ten accompanying vegetation units. In between S. commixta and life-farms, 120 correlations were positive with 23 of these having a p-value < 0.01 for trees, 21 for shrubs, 10 for woody vines, and 25 for herbs. In trees, there was a positive correlation between S. commixta and A. takesimense and T. insularis on the $1\%$ level. In shrubs, there was a positive correlation between S. commixta and Sasa kurilensis, Callicarpa japonica, Ligustrum foliosum on the $5\%$ level. In woody vines, there was a negative correlation between S. commixta and Tripterygium regelii and Actinidia rufa on the $1\%$ level. In herbs, there was a positive correlation between S. commixta and Majanthemim dilatatum and Solidago virga-aurea var. gigantea on the $1\%$ level.

• Korean Journal of Food Science and Technology
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• v.36 no.3
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• pp.484-489
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• 2004

Bifidobacterium species isolated from infant feces were fractionated into cell wall, cytosol, and extracellular preparations of culture broth, and each fraction was examined for Peyer's patch-mediated bone marrow cell proliferation activity in vitro. Cell wall preparation of B. bifidum SL-21 (CWP) showed the highest bone marrow cell proliferating activity dose dependently, and enhanced production of cytokines, such as hematopoietic growth factor (GM-CSF), IL-2, and IL-6, in culture supernatant of Peyer's patch cells, After treatment with lysozyme, CWP was fractionated, among which intermediate molecular-weight fraction (30-50 kDa) showed significantly high bone marrow cell proliferating activity. These results suggest CWP of B. bifidum SL-21 effectively activates lymphocytes in Peyer's patch, and several cytokines, possibly playing important role in enhancement of systemic immune system, were produced by activated lymphocytes.