• KSBB Journal
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• v.4 no.3
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• pp.266-270
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• 1989

Insect cell cultures were performed in laboratory-scale vessels. The batch growth of insect cells was affected by such parameters as serum content, other nutrients, seeding density, and mechanical agitation. Lactate and ammonium were not likely to be environmental factors that inhibited cell growth at the concentrations observed at the end of batch cultures. In addition, redox potential was found to be a useful index in monitoring low-level dissolved oxygen during the cultivation of insect cells. Recombinant protein production by cells infected with a genetically-modified baculovirus was also demons treated. The maximum beta-galactosidase synthesis of 2800 units per reactor volume was achieved at the dilution rate of $0.006hr^{-1}$.

• Korean journal of applied entomology
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• v.38 no.2
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• pp.123-128
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• 1999

Recently, entomopathogenic nematodes have received a considerable attention as biologicalcontrol agents. For in vitro cultivation, storage and transportation of nematodes, oxygen supply isextremely impotant due to its limited solubility and mass transfer problem. The oxygen uptake rates(OURs) of four different Steinernema species were measured in a 5L bioreactor at varying temperatures.The OURs of the Steinernema spp. were below 0.5 x mmolO'||'&'||' . min in the range of 13-17$^{\circ}$C. TheOURs (mmo102/L - min) of S. glaseri Dongrae and S. carpocapsae Pocheon strains were 0.4 x lo-', 0.75x lo-\ulcorner at 21$^{\circ}$C, 1.5 x lo-\ulcorner, 3.2 x 10-2 at 25"C, and 2.8 x lo-', 5.8 x lo-\ulcorner at 29"C, respectively. However,the OURs were not significantly altered by the agitation speed of 50-150 rpm. The specific oxygenuptake rates (qol) of S. glaseri NC, S. glaseri Dongrae, S. glaseri Mungyeong and S. carpocapsaePocheon strains were 0.3 x 0.5 x 0.3 x and 0.2 x mmolO~/cell min at 25"C,respectively. As the nematode size and temperature were increased, the qo, was also increased.the qo, was also increased.

• Korean Chemical Engineering Research
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• v.51 no.5
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• pp.609-614
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• 2013

This study investigated the spontaneous combustion behavior of solvent-treated low rank coals. Indonesian lignite (a KBB and SM coal) and sub-bituminous (a Roto coal) were mixed with non-polar 1-methyl naphthalene (1MN) either by mechanical agitation or ultrasonication. The property change associated with 1MN treatment was then analyzed using proximate analysis, calorific value analysis, Fourier transform infrared (FT-IR), X-ray photoelectron spectroscopy and moisture re-adsorption test. Susceptibility to spontaneous combustion was evaluated using crossingpoint temperature (CPT) measurement along with gas analysis by GC. A FT-IR profile showed that oxygen functional groups and C-H bonding became weaker when treated by 1 MN. XPS results also indicated a decrease of the oxygen groups (C-O-, C=O and COO-). Increased hydrophobicity was found in the 1MN treated coals during moisture readsorption test. A CPT of the treated coals was ${\sim}20^{\circ}C$ higher than that of the corresponding raw coals and the ultrasonication was more effective way to enhance the stability against spontaneous combustion than the agitation. In the gas analysis less CO and $CO_2$ were emitted from 1MN treated coals, also indicating inhibition of pyrophoric behavior. The surface functional groups participating in the oxidation reaction seemed to be removed by the ultrasonication more effectively than by the simple mechanical agitation.

• The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.10 no.6
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• pp.62-72
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• 1996

In this paper, ozonizer using U-type lamp(Olamp) has been designed and manufactured, which can perform a role of lighting source and ozonizer by using photo and chemical methods. The discharge, spectrum, illuminance, ozone concentration, ozone generation, ozone yield and sterilization characteristics of Olamp have been studied. The important conclusions obtained from this paper can be summa'||'&'||'not;rized as follows. As a result of spectrum characteristics for Olamp, ultraviolet ray of a short wave'||'&'||'not;lengths and a visible ray are radiated. The illuminance of Olamp was found to be useful for "color distinctive and intermittent works in the dark working spaces" in accordance with KS A 3011. The ozone concentration of gaseous phase is inversely proportional to quality of supplied gas. Also, ozone conce tration and generation of gaseous phase are rised more commercial oxygen gas than those trial air gas for constant quality of supplied gas. Ozone generation and ozone yield of gaseous phase are proportion'||'&'||'not;al to ozone concentration of gaseous phase. The characteristics of liquid ozone concentration at distilled water are proportional to circulating velocity of fermentation chamber and ozone concentration of gas'||'&'||'not;eous phase. As a result, the sterilization characteristics of Escherichia coli have been obtained more than 97[ % J.