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Comparison of Callus Formation Ratios from Seed Explants, Callus Sizes and Regeneration Efficiency Among Several Ochardgrass (Dactylis glomerata L.) Varieties (오차드그래스(Dactylis glomerata L.)의 품종에 따른 종자유래의 캘러스 형성률과, 캘러스 크기 및 식물체 재분화 효율의 비교)

  • 배은경;이인애;김기용;이병현;손대영;이효신;정민섭;조진기
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.22 no.2
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    • pp.93-100
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    • 2002
  • Comparison results of callus formation ratio from seed explants, callus sizes, regeneration ratios from callus and regeneration efficiency [calculated by following formular; callus formation ratio(%) ${\times}$ regeneration ratio(%)/100] for 27 ochardgrass (Dactylis glomerata L.)varieties imported and Hapsung 2 developed in Korea are as follows; 1. Among ochardgrass varieties showing more than 50% callus formation ratios, the descending order of callus formation ratio after bedding the seed explants for 4 weeks was 93M>Sparta> Pizza>Condor>Lidaglo>Glorus>Hapsung 2>Frode. 2. The callus sizes after bedding for 4 weeks were in the range of $\Phi$ 0.43cm~4.2cm in which there was 10 times size difference between the largest one and the smallest one but most of them were between ${\Phi}2.5cm~4cm$. 3. The regeneration ratio from callus among varieties were in the range of 0~36% and descending order of the upper 6 varieties was Plano>Akimidori>Justus>Lidacta>Currie>Hall mark. 4. The regeneration efficiency which is calculated by the ratios of regeneration from seed explant numbers was between 0 to 17.4% among which Justus showed the highest value in the 4-week treatment. 5. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5765, r=0.6365 and r=0.6246, respectively in 4-week callus and all the correlations were significant on the 1% level. 6. In 6-week callus, the descending order callus formation ratios from seed explants fur the best 6 varieties was Condor>Sparta>93 M>Justus>Potomac>Lidaglo>Frode. 7. The callus sizes formed were between ${\Phi}1.5~5.7cm$ in which Sparta, the largest one of ${\Phi}5.7cm$ was five times larger than the smallest one. The callus size of the control variety, Hapsung 2 was ${\Phi}3.8cm$, which belonged to a larger size. 8. Regeneration ratio showed a great deviation among varieties from 6-week old calli by showing from 0% to 100% in which all the calli were regenerated in Piano while no callus was regenerated in Juno. 9. The range of regeneration efficiency was between 0~28% among varieties in which the values from 6-week callus treatment were larger than those from 4-week callus treatment. Especially, the value of Potomac in 6-week was 3 times larger than that in 4-week. 10. The correlation between callus formation ratios and the callus sizes, callus formation ratios and regeneration efficiency, and callus sizes and regeneration efficiency were r=0.5369, r=0.6683 and r=0.5937. respectively in 6-week callus, and all the correlations were significant on the 1% level.

The Effect of Long-Term Fertilization on the Yield and Quality of Soybean (동일비료 24년 연용이 콩의 수량(收量) 및 품질(品質)에 미치는 영향(影響))

  • Park, No-Kwaun;Kim, Jae-Kyu;Seo, Young-Jin;Park, Seon-Do;Choi, Boo-Sull
    • Korean Journal of Soil Science and Fertilizer
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    • v.31 no.4
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    • pp.330-333
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    • 1998
  • This experiment was conducted to investigate the effect of long-term fertilization on the yield and quality of soybean. The yield of soybean was highest in the NPK+lime $1.5Mg\;ha^{-1}$ treatment. Carbohydrate content of soybean was high in the non-fertilizer treatment and crude protein content was high in the NPK+lime $1.5Mg\;ha^{-1}$ and NPK+silicate $1.5Mg\;ha^{-1}$ treatment, and crude lipid and ash content was high in the NPK+silicate $1.5Mg\;ha^{-1}$ treatment. The content of inorganic compounds were not different among treatments. Correlation between $P_2O_5$/T-N and carbohydrate, crude lipid was $-0.99^{**}$, $-0.94^{**}$ respectively. Correlation between Mn and carbohydrate was $0.95^*$, but others were not significantly different. Yield of soybean increased with lime application and protein content increased with silicate application.

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Surface Ultrastructure of Heterophyes nocens (Trematoda: Heterophyidae) (Heterophues nocens (Trematoda: Heterophyidae)의 표피 미세구조)

  • 채종일;정혜림
    • Parasites, Hosts and Diseases
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    • v.30 no.2
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    • pp.75-82
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    • 1992
  • The surface ultrastructure of Heterephyes nocens (Trematoda: Heterophyidae) was studied by scanning electron microscopy(SEM), The adult worms were recovered from experimentally infected cats and from a naturally infected patient. They were leaf-like, ventrally concave, and ovoid or lyriform in shape. Ciliated knob-like sensory papillae (type I) were observed in single or grouped forms on and around the oral sucker, whereas non-ciliated round swellings (type II papillae) were seen on the lip of the ventral sucker, The tegumental spines around the oral sucker were 5∼9 pointed, whereas those between the two suckers were 12∼17 pointed. yentrolaterally, three groups of 5∼6 type I papillae were located between the oral and ventral suckers, with single ones alternating between them. The genital sucker was protruded or depressed, depending on the contraction state of the nukes, and the gonotyl spine number ranged 50∼60. The number of tip points of tegumental spines was decreased posteriorly; finally they became 1∼3 pointed. On the dorsal surface, 4 groups of 4∼5 type I papillae were symmetrically located on both lateral sides, and the shape and distribution of tegumental spines were similar to those of the ventral surface. Although the tegumental ultrastructure of H. nocens was generally similar to those of other heterophyids, the genital sucker morphology including the number of gonotyl spines and/or the distribution pattern of tegumental spines and sensory papillae were suggested to be the characteristic features of H. necens.

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Effect of Sodium Butyrate on Blood Glucose, Serum Lipid Profile and Inflammation in Streptozotocin-induced Diabetic Mice (스트렙토조토신으로 유도한 당뇨마우스에서 Sodium Butyrate의 혈당, 혈청 지질 성상 및 염증 억제에 미치는 영향)

  • Yun, Jung-Mi
    • The Korean Journal of Food And Nutrition
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    • v.28 no.2
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    • pp.171-177
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    • 2015
  • Sodium butyrate is a short-chain fatty acid derivative found in foods, such as Parmesan cheese and butter and is produced by anaerobic bacteria fermentation of dietary fibers in the large intestine. There have been reports that butyrate prevented obesity, protected insulin sensitivity, and ameliorated dyslipidemia in dietary obese mice. This study investigated the effects of sodium butyrate on fasting blood glucose level and serum lipid profile in streptozotocin(STZ)-induced diabetic mice. Male C57BL/6 mice were fed AIN-93G for four weeks prior to intraperitoneal injections with STZ (100 mg/kg body weight). Diabetic mice had supplements of 5% sodium butyrate for four weeks. The 5% sodium butyrate diet significantly improved fasting blood glucose level and lipid profile in STZ-induced diabetic mice. Inflammation has been recognized to decrease beta cell insulin secretion and increase insulin resistance. Circulating cytokines can directly affect beta cell function, leading to secretory dysfunction and increased apoptosis. Thus, anti-inflammatory therapies represented a potential approach for the therapy of diabetes and its complications. In this animal study, the 5% sodium butyrate supplementation also inhibited inflammatory cytokine production in STZ-induced diabetic mice. These results suggested that sodium butyrate can be a potential candidate for the prevention of diabetes and its complications.

Intermetallic Formation between Sn-Ag based Solder Bump and Ni Pad in BGA Package (BGA 패키지에서 Sn-Ag계 솔더범프와 Ni pad 사이에 형성된 금속간화합물의 분석)

  • Yang, Seung-Taek;Chung, Yoon;Kim, Young-Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.9 no.2
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    • pp.1-9
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    • 2002
  • The intermetallic formation between Sn-Ag-(Cu) solders and metal pads in a real BGA package was characterized using SEM, EDS, and XRD. The intermetallic phase formed in the interface between Sn-Ag-Cu and Au/Ni/Cu pad is likely to be ternary compound of $(Cu,Ni)_6Sn_5$ from EDS analysis High concentration of Cu was observed in the solder/Ni interface. XRD analysis confirmed that $\eta -Cu_6 Sn_5$ type was intermetallic phase formed in the interface between Cu containing solders and Ni substrates and $Ni_3$Sn_4$ intermetallic was formed in the Sn-Ag solder/Ni interface. The thickness of intermetallic phase increased with the reflow times and Cu concentration in solder.

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A Study on Compatibility between DTV and CDMA System (DTV와 CDMA 시스템간의 양립성 분석에 관한 연구)

  • Cheng, Yan-Ming;Lee, Il-Kyoo;Shim, Yong-Sup;Kim, Jong-Tae;Lee, Kyoung-Kun
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.10 no.3
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    • pp.75-82
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    • 2010
  • Korea has made a plan to allocate CH 14~CH 51 (470 MHz~698 MHz) for DTV. This paper assumes that DTV operates on CH 51 (692 MHz~698 MHz) and CDMA system operates on CH 52 (698 MHz~704 MHz) in spare band. Minimum Coupling Loss (MCL) method to get protection distance and Spectrum Engineering Advanced Monte Carlo Analysis Tool (SEAMCAT) to get guard band through 5 % interference probability are used. The protection distance is required to be 665.67 km at close frequency offset of 698.625 MHz between DTV transmitter and CDMA Base Station (BS) receiver. The required guard band between DTV transmitter and CDMA Mobile Station (MS) receiver is 5 MHz for the worst case of rural environment. There is no serious impact between CDMA MS transmitter and DTV receiver. The required guard band between CDMA BS transmitter and DTV receiver is 6.25 MHz for the worst case of urban environment. The analysis results may offer a reference and be helpful for considering interference between DTV and other communication systems.

Miniscrew Installation Area and Condition on Maxillary Palatal Side (상악구개측 미니스크류 식립위치 및 조건)

  • Lee, Ki-Yeon;Lee, Jin-Woo
    • Journal of Dental Rehabilitation and Applied Science
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    • v.25 no.1
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    • pp.61-71
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    • 2009
  • Anchorage control is important in orthodontic treatment. Recently miniscrew is widly used as maximum anchorage in orthodontic treatment, and then it is important to install miniscrew safely without damaging adjacent anatomic structure. In a view of Miniscrew's stability, maxilla is unfavorable than mandible, and moreover maxillary soft buccal bone has disadvantage on stability. so palatal area comes into notice for installation area. We measured distance between palatal roots and bone thickness at midpalatal area using 3D computed tomography, and have found following results. 1. On the comparison of distance between palatal roots, the distance between 2nd premolar and 1st molar was significantly longest and the distance between premolars was significantly shortest. 2. Going toward lateral area from midpalatal suture and posterior area from zero point, bone thickness significantly became shorter and shorter. And 5.0mm palatal sagittal plane has more significance decrease of bone thickness than 2.5mm palatal sagittal plane. According to these results, we can conclude that the palatal installation of miniscrew between 2nd premolar and 1st molar is safest. And it is more safe that comes closer to midpalatal suture and to anterior area in regard to incisive canal.

Demanufacturing Technology for Recycling (리사이크링을 위한 역제조기술)

  • Lee, Hwa-Jo
    • Journal of the Korean Society for Precision Engineering
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    • v.17 no.8
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    • pp.5-14
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    • 2000
  • The treatment of technical consumer products after using is important for the conservation of our environment. In this paper the demanufacturing technology that is required to maximize the recycling rate of end-of-life products is introduced, The definitions including types of the recycling are described. The factors that have to regard in the design stage of products in order to maximize the recycling rate of the products are introduced. The objective assessment methods for the design for environment are described. Finally disassembly process and future prospect of the automations are discussed.

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