• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.297-307
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• 1995

Anatomical and physiological studies of sink tissues are required for better understanding the biological plant growth system and energy metabolism Anatomy of root growth zones of two genotypes of tall fescue (Festuca arundinacea Schreb.) receiving 50 or 200 ppm N were determined, Cross-sectional anatomy and cells responses of root growth zones were observed and examined. Rapid radial root expansion occurred within the first 1.0 mm from root apex, and then increased gradually for both genotypes and N levels. Another increase in diameter occurred at high N after cell elongation slowed near 3.0 mm. Area of the central cylinder cell increased rapidly near the root apex. However, it then decreased again about 1.0 to 1.5 mm from the apex, perhaps because of pressure from the rapid increase of root diameter due largely to an increasing proportion of cortex and epidermis or hypodermis in the distal portion of the root growth zone. Root area from the apical initial to 6.0 mm distal consisted of 10 to 18% epidermis or exodermis, 67 to 79% cortex, and 10 to 22% vascular cylinder cells containing cambium cells (6 to 20%) and xylem cells (0.8 to 2.5%). These data indicate that N application affects root growth radially by increasing mainly cortex cell area, with less effect on epidermis and central cylinder cells.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.65 no.3
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• pp.192-198
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• 2020

Root extension is the most important growth change that occurs during cultivation. We analyzed growth changes according to young seedling age, temperature, and the degree of root cutting in order to identify factors affecting rooting after transplanting. Root cutting did not affect plant height growth rate, root growth rate was increased in plants that experienced root cutting, and 14-day-old seedlings exhibited a higher growth rate than 7-day-old seedlings. Growth temperature experiments revealed that elongation was high at 25℃ and 28℃, but tended to be low at 18℃, and root elongation was high at 22-28℃ for 7-day-old seedlings and 22-25℃ for 14-day-old seedlings. Nitrogen absorption decreased in the following growth temperature order: 25, 28, 22, 18℃, and differences in nitrogen absorption under different growth temperatures tended to be lower in 7-day-old seedlings. The amount of nitrogen taken up by roots did not differ significantly between the short root treatment and the control, and 7-day-old seedlings tended to start nitrogen absorption faster than 14-day-old seedlings. Root vitality was highest in short-rooted 7-day-old seedlings with 3 cm of root remaining, and vitality also tended to be high in short-rooted 14-day-old seedlings.

• Korean Journal of Environmental Agriculture
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• v.18 no.2
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• pp.185-190
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• 1999

Autotoxic substance(s) from alfalfa(Medicago sativa L.) plants reduces germination and growth of adjacent new alfalfa after alfalfa. The autotoxic chemical(s) in alfalfa are clearly unknown. Our objective was to improve the sensitivity of an alfalfa seedling bioassay for evaluating autotoxic leaf extracts. We determined critical extract concentrations that inhibit seed germination and seedling growth, compared two different culture media, and evaluated the effects of extract sterilization on the sensitivity of the assay, by using streptomycin and autoclaving method. An agar medium in petri plate gave better responses of germination and seedling growth to the extracts than using filter paper in the plate. On agar medium, the concentration of extract required to reach 50% inhibition of root length was 2.7 g $kg^{-1}$, and of germination and hypocotyl length were 3.8 and 9.9 g $kg^{-1}$, respectively. Leaf extracts with 100 ppm streptomycin stimulated germination significantly compared to Leaf extract alone but reduced root length of control by 43%. Root length was more sensitive to the autotoxin(s) than was germination or hypocotyl length. These results suggest that agar medium mixed with extract and sterilization by autoclaving could be improved the consistency and precision of bioassay, and that root length was the best parameter of autotoxic effect of alfalfa leaf extract.

• Journal of Life Science
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• v.22 no.10
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• pp.1371-1377
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• 2012

Fibrosis in kidney by internal and external factors causes progressive loss of renal function. Renal fibrosis is the inevitable consequence of an excessive accumulation of the extracellular matrix. TGF-${\beta}$ plays an important role in the process of renal fibrosis and stimulates the synthesis of profibrotic factors, including collagens, fibronectin, and plasminogen activator inhibitor (PAI-1). We examined the effect of Moringa oleifera Lam (moringa) extracts in a rat kidney fibrosis model. We found that moringa root extract suppresses protein expression/mRNA levels of Type I collagen, fibronectin, and PAI-1 induced by TGF-${\beta}$ in renal fibroblasts. Moringa root extract selectively inhibited phosphorylation of TGF-${\beta}$-induced $T{\beta}RII$ and the downstream signaling pathway (e.g., Smad4), and phospho-ERK, but not JNK, p38, or PI3K/AKT. These results suggest that moringa root extract can act against TGF-${\beta}$-induced renal fibrosis in rat kidney fibroblast cells by a mechanism related to its antifibrotic activity, which regulates expression of fibronectin, Type I collagen, and PAI-1 through $T{\beta}RII$-Smad2/3-Smad4 and ERK. Therefore, moringa root extract is an effective substance for fibrosis therapy and provides a new therapeutic strategy for diseases associated with elevated profibrotic factor synthesis.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.40 no.3
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• pp.285-296
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• 1995

Anatomical studies of sink tissues are required for better understanding the biological plant growth system and energy metabolism. Kinematics of root growth zones of two genotypes of tall fescue (Festuca arundinacea Schreb.) receiving 50 or 200 ppm N were determined. Longitudinal anatomy and cell dynamics of root growth zones were studied and calculated. The root growth zone is organized similarly to the leaf growth zone which has cell division, elongation, and maturation zones, but the root growth zone is only about 3.0 mm long compared to 25 to 30 mm for the leaf growth zone. The root cap extends about 0.4 to 0.5 mm from the apical initial, while the cell elongation zone for both cortical and metaxylem cells extends about 3.3 mm from the apical initial for both genotypes and N levels. Root cap cells elongate from an initial length of about 5$\mu{m}$ long to a final length of about 40$\mu{m}$ before being sloughed. Initial lengths of cortical and metaxylem cells were about 8.5 $\mu{m}$ and 13.0 $\mu{m}$, respectively. Elongation of cortex and metaxylem cell showed sigmoidal curves with final lengths of about 120 $\mu{m}$ for cortex cells and 650 $\mu{m}$ for metaxylem cells. Initial size and final size for both types were not affected by N level, but cell fluxes and cell elongation rates of cortical and metaxylem cells were about double in low N. Cell production rates were about 5 to 6 times higher in cortical cells than in metaxylem cells. Differences in N caused a larger change in cell production rate, duration of cell elongation, and relative cell elongation rate than did the genotypes. These data indicate that N application affects root growth longitudinally by changing cell production rate and elongation rate.

• Korean Journal of Soil Science and Fertilizer
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• v.10 no.1
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• pp.7-12
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• 1977

This experiment was conducted in the laboratory in order to find out the relationships between the root growth and soil physical properties. The soils selected for this study were Sangju sandy loam, Yeongog loam, Hwadong silty clay loam, which have been considered to be a typical upland soils of Korea. Artificial core samples were made with various moisture contents and bulk densities. Elongation rate of pea seedling taproot and soil strength were measured respectively in these core samples. The results obtained are summarized as follows: 1. The soil strength increased with the bulk density and deceased with moisture content. 2. The correlation between root elongation and soil bulk density was significantly recognized at the same moisture content and the root elongation was influenced by the bulk density more significantly at dry condition. 3. The elongation rate of pea seedling taproot was significantly decreased by increasing the strength (Yamanaka tester and Fine probe) of the soils. 4. The soil strength of $21kg/cm^2$ in fine metal probe or 24mm in Yamanaka tester was considered to be the critical point for plant growth, which was restricting root elongation smaller than 1/4 of the maximum growth rate.

• Journal of Life Science
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• v.25 no.11
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• pp.1223-1229
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• 2015

Oryzalin is a dinitroaniline herbicide, which disrupts the arrangement of microtubules. Microtubules and microfilaments are cytoskeletal components that are thought to play a role in the sedimentation of statoliths and the formation of cell walls. Statoliths regulate the perception of gravity by columella cells in the root tip. To determine the effect of oryzalin on the gravitropic response, ethylene production in primary roots of maize was investigated. Treatment with 10^-4 M oryzalin to the root tip inhibited the growth and gravitropic response of the roots. However, the treatment had no effect on the elongation zone of the roots. An application of 10^-4 M oryzalin for 15 hr to the root tip caused root tip swelling. The application of 1-aminocycopropane-1-carboxylic acid (ACC), a precursor of ethylene, to the root tip also inhibited the gravitropic response. To understand the role of oryzalin in the regulation of the growth and gravitropic response of roots, ethylene production in the primary roots of maize was measured following treatment with oryzalin. Oryzalin stimulated ethylene production via the activation of ACC oxidase (ACO) and ACC synthase (ACS), and it increased the expression of ACO and ACS genes. Indole-3-acetic acid (IAA) played a key role in the asymmetric elongation rates observed during gravitropism. The results suggest that oryzalin alters the gravitropic response of maize roots through modification of the arrangement of microtubules. This might reduce the distribution of IAA in the upper and lower sides of the elongation zone and increase ethylene production, thereby inhibiting growth and gravitropic responses.

• Korean Journal of Soil Science and Fertilizer
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• v.45 no.3
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• pp.332-338
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• 2012

Soil compaction is one of the major problems facing modern agriculture. Overuse of machinery, intensive cropping, short crop rotations, intensive grazing and inappropriate soil management leads to compaction. This study was carried out evaluate of the effects soil texture and different compaction levels within the soil profile on the soybean root growth and productivity. The soybean plants were grown in $21cm{\o}{\times}30cm$ cylinder pots using three different soil textures (clay, fine loamy and coarse loamy) compacted at different compaction levels (1.25, 1.50, 1.75, and 2.00 MPa). Results revealed that soybean development is more sensitive on penetration resistance, irrespective of soil type. Soybean yield and root weight density significantly decreases with increasing levels of soil compaction in both clayey and fine loamy soils, but not in coarse loamy soil. The highest root weight density was recorded in coarse loamy soils, followed by fine loamy and clay soils, in descending order. The root growth by soil compaction levels started to decline from 1.16, 1.28 and 1.60 MPa for clay, fine loamy and coarse loamy soils. Soybean production in the field experiment decreased about 30% at compacted sub-soils compared to undisturbed soils.

• Journal of Environmental Science International
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• v.5 no.6
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• pp.813-826
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• 1996

The toxic effects of aluminium (Al) on growth, chlorophyll content, $\delta-aminolevulinic$ acid dehydratase (ALAD) activity and anatomy of root and shoot were investigated in 7-day-old azuki bean (Vigna angularis) seedlings. Significant depressions in root elongation was observed in the low concentrations of Al (50, 100 $\muM)$ and increasing Al concentrations caused a sharp decline of root and shoot growth. The degree of inhibition was dependent upon Al supply. Exposure to 50 $\muM$ Al or more inhibited root elongation within 1 day. In the 50 $\muM$ Al treatments, a recovery of root growth was seen after 7 days exposure. In contrast, lateral root initials was little affected by Al exposure. Al toxicity symptoms and growth responses were more well developed in the roots than in the shoots. Analysis of Al localization in root cells by hematoxylin stAlning showed that Al entered root apices and accumulated in the epidermal and cortical cells immeadiately below the epidermis. There was a good positive correlation between the level of chlorophyll and ALAD activity. Increasing Al concentrations caused a decrease in total chlorophyll contents, accompanied by proportional changes in ALAD activity, suggesting a cootr-dinated reduction of a photosynthetic machinery. Al exerted specific influence on the morphology of root ann shoot. At higher concentrations of Al the roots induced drastic anatomical changes. The epidermal cells were disorganized or destructed while the cortical cells exhibited distortion of cell shape and/or disintegration. The diameter of root and transectional area of cortical cells decreased considerably with Al treatment. In the shoot Al also enhanced reduction of diameter of shoot and cell size. Gross anatomy of leaves treated with Al did not differ significantly from the controls, except for fewer and smaller chloroplast. Our results indicate that toxic effect of Al appear to be manifested primarily in roots and secondarily on shoots, and changes in root morphology are related to changes in the root growth patterns. Results are further discussed in re181ion to the findings in other plant species, and it is concluded that Al causes morphological, structural and, presumably, functional damage to the roots of the species investigated.

• Journal of Life Science
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• v.14 no.5
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• pp.809-814
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• 2004

To investigate the effect of the electrolyzed acidic water for soybean sprouts growth, the responses of characteristics of soybean sprouts were evaluated. Soybean sprouts grown by the electrolyzed acidic water showed shorter length in total body, root, and hypocotyl, etc. but they were evaluated to be increased in hypocotyl diameter and weight per sprout. Total length of soybean sprouts grown for 5 days by electrolyzed acidic water were much shorter than those by tap water. Soybean sprouts grown by tap water showed rapid growth in length even after 5 days but no more growth in length for those grown by electrolyzed acidic water. The growth of hypocotyl showed the same tendency as total length. No difference in root length among the soybean sprouts grown for 4 ~ 11 days by electrolyzed acidic water while those grown by tap water showed continuous rapid growth in length. The diameter of hypocotyl was thicker in those grown by electrolyzed acidic water than those grown by tap water and increased up 5 days. The weight of cotyledon grown by electrolyzed acidic water showed the proportional increase to the growing days but those grown by tap water showed no increase in hypocotyl weight up to 7 days, but a little bit increase after 11 days with the growth of new buds. The fresh weight per sprout was higher in those grown by electrolyzed acidic water until 7 days than tap water but it was the same weight in 11 days cultivation. The electrolyzed acidic water effected on shortening of hypocotyl and root length, thickening of hypocotyl diameter, and enlarging of cotyledon during soybean sprout cultivation.