• Korean journal of applied entomology
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• v.53 no.1
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• pp.85-91
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• 2014

In this study, we analyzed the phylogenetic characterization of root-knot nematodes (Meloidogyne spp.) in soils from fruits and vegetables greenhouses in Korea. Soil samples were collected from 12 greenhouse fields in which tomato, cucumber, watermelon, and Oriental melon were being cultivated. Meloidogyne spp. were detected in all the soil samples at an average number of $72{\pm}6$ nematodes/300 g of soil to $2,898{\pm}468$ nematodes/300 g of soil. Phylogenetic analysis using polymerase chain reaction-restriction fragment length polymorphism was attempted for the second-stage juveniles (J2) of Meloidogyne spp. collected from the greenhouse soils. Twelve Meloidogyne spp. from the greenhouse soils were classified into two groups by using HinfI digestion of mitochondrial DNA, resulting in 900, 410, 290, and 170 bp fragments (group A) and 900, 700, and 170 bp fragments (group B). Phylogenetic analysis based on mitochondrial DNA sequences (1,483-1,521 bp) showed that nine group A isolates were identified as Meloidogyne incognita (99.73-99.93%) and three group B isolates showed 99.54-99.73% similarity to Meloidogyne arenaria.

• Journal of the Korean Society of Food Science and Nutrition
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• v.41 no.4
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• pp.526-532
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• 2012

This study was conducted to investigate chemical properties of wild $Lactuca$ $indica$ (WL) and cultivated $Lactuca$ $indica$ (CL). The proximate composition, reducing sugar, free amino acids, organic acid, vitamin C, minerals, chlorophyll, and crude saponin were analyzed. WL and CL contained high levels of carbohydrate. The leaves and roots of CL contained higher levels of free amino acid than those of WL. Especially, the proline content of CL leaf was 12 times higher than that of WL leaf, and the arginine content of CL root was 100 times higher than that of WL root. The major organic acid and mineral of $Lactuca$ $indica$ were tartaric acid and potassium, respectively. CL showed significantly higher value of reducing sugar than WL. The vitamin C content of the samples ranged from 0.4 to 24.1 mg%, and CL leaf was the highest amount of vitamin C among the samples. CL leaf had a higher amount of chlorophyll than WL leaf, but WL root contained a higher amount of crude saponin than CL root. As in this study, CL showed better nutritional properties than WL, and these results will provide fundamental data in order to activate the cultivation of wild plants.

• Korean Journal of Environment and Ecology
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• v.25 no.1
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• pp.31-36
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• 2011

To provide the basic information about slope stability analysis, tensile force and strength of tree roots like Pinus koraiensis, Larix leptolepis, Pinus densiflora, Quercus mongolica, and Alnus japonica were measured and analyzed. As a result, tensile force increases in forms of involution of root diameter. The mean tensile strength of roots like P. koraiensis, L. leptolepis, P. densiflora, A. japonica and Q. mongolica were calculated as $165.38kgf/cm^2$, $172.78kgf/cm^2$, $176.25kgf/cm^2$, $214.29kgf/cm^2$ and $224.19kgf/cm^2$ respectively. It was shown that tensile strength decreasing tendency as root diameter increases. Also, recalculated soil shear strength by tensile strength of the roots like P. koraiensis, L. leptolepis, P. densiflora, A. japonica and Q. mongolica were $0.099kgf/cm^2$, $0.104kgf/cm^2$, $0.106kgf/cm^2$, $0.129kgf/cm^2$ and $0.135kgf/cm^2$ respectively.

• Journal of Applied Biological Chemistry
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• v.59 no.1
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• pp.5-7
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• 2016

One of oriental medicinal plants, Potentilla chinensis, has been used for anti-inflammation, hemostatic, decryption, and antipyretic. Especially, a root of Potentilla chinensis was used as important material for oriental medication. Although several kinds of bioactive component of Potentilla chinensis extract from stems and leaves were identified, the major component of Potentilla chinensis from roots is not well established. In this study, the root of Potentilla chinensis was extracted in different solvent system and analyzed by high performance liquid chromatography (HPLC). According to HPLC analysis, a major component was isolated and its physicochemical properties were evaluated by mass spectrometry and nuclear magnetic resonance. Based on these results, isolated compound was identified as 2,3,8-Tri-O-methylellagic acid. And quantification of 2,3,8-Tri-O-methylellagic acid with different extraction solvent system was performed for industrial application.

• The Korean Journal of Mycology
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• v.47 no.3
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• pp.199-208
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• 2019

In this study, the community structures of the ectomycorrhizal (ECM) fungi in the roots of Abies koreana and Taxus cuspidata were investigated at different altitudes of Mt. Halla. We identified the collected ECM root tips based on morphological characteristics and phylogenetic analysis through sequencing of the rDNA ITS regions. From the roots of A. koreana and T. cuspidata, 11 species and 12 species were identified, respectively. The Shannon's index and species evenness and abundance of the ECM fungi were higher in the higher than lower regions, regardless of host plant species, however, the number of ECM root tips showed the opposite pattern. The community similarity among the ECM fungi in A. koreana was significantly higher than that among the ECM fungi in T. cuspidata or than that between A. koreana and T. cuspidata (p < 0.05). These results could be useful for the conservation and management of the habitat of A. koreana, which is threatened with extinction due to increasing ambient air temperature.

• Korean journal of applied entomology
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• v.46 no.1 s.145
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• pp.169-173
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• 2007

Root-knot nematode damage was found on yam, Dioscorea bulbifera in Andong Korea. From the root-knots, female nematodes were isolated and subjected to DNA sequence analysis. Sequence of cytochrome oxidase subunit II (COII) was analyzed from the genomic DNA of the isolate. COII locus size and sequence of the nematode isolate were similar to those of Meloidogyne javanica or M. incognita. However, an analysis of HinfI restriction site, a species-specific character between these two species, showed that the isolate did not match to either M. javanica or M. incognita.

• Journal of Life Science
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• v.23 no.11
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• pp.1381-1387
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• 2013

The leaves and roots of Ligularia stenocephala, which are widely used as a food in Korea, were investigated for their antioxidant activities and cytotoxicity in vitro, and their hepatoprotective effect, alcohol detoxicant efficacy, and memory-enhancing property were investigated in vivo. The unique odor of the leaves was analyzed by GC-MS. Lipid peroxidation, superoxide anion formation, and DPPH radicals were inhibited remarkably by the extracts of the leaves and roots. The leaves of this edible plant significantly protected the hepatotoxicity induced by carbon tetrachloride and further diminished the blood alcohol content in mice. While the roots of this plant exhibited adequate cytotoxicity against four human tumor cell lines, especially against melanoma, the leaves revealed relatively weak activity. Both the leaves and the roots exerted an excellent ameliorating property on scopolamine-induced memory impairment in the passive avoidance task using an animal model. The hexane fraction of the leaves was analyzed by GC-MS, suggesting that a series of terpenoids may be odorous compounds in this plant.

• Research in Plant Disease
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• v.25 no.1
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• pp.33-37
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• 2019

From 2016 to 2018, approximately 15% of kohlrabi were observed displaying significant clubroot symptoms in farmer's fields in Jeju, Korea. The initial infection appeared as hypertrophy of root hairs, and as the disease progressed, galls formation occurred on the main roots, finally disease progress resulted in yellowing and wilting of leaves. Pathogenicity was proven by artificial inoculation of plants with resting spore suspension, fulfilling Koch's postulates. The resting spore is one-celled, spherical and subspherical, colorless, and $3-5{\mu}m$ in diameter. On the basis of the morphological characteristics and phylogenetic analyses of internal transcribed spacer rDNA, the causal agent was identified as Plasmodiophora brassicae. To our knowledge, this is the first report on the occurrence of P. brassicae on kohlrabi in Korea.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2002.02a
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• pp.573-578
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• 2002

양파박피기 개발을 목적으로 수행된 본 연구에서는 공정상 필요한 뿌리절단장치에 관하여 시험 분석하였다. 뿌리를 절단, 제거하는 데 적합한 칼날의 종류, 회전속도와 이송속도, 및 소요동력 등 제 특성을 구명하여 적절한 회전칼날을 제작 사용함으로써 능률 높은 양파박피기를 개발하고자 수행한 결과를 요약하면 다음과 같다. 1. 양날형, 축날형, 원추형, 및 원통형 등 직경 30 mm의 칼날을 제작하여 실험하였던 바, 양파의 뿌리절단에는 구부린 칼날로 파내기를 하고 수직칼날로 금긋기를 동시에 할 수 있는 양날형이 가장 좋은 것으로 나타났다. 2. 양파의 뿌리부가 100% 절단이 되고 소요동력이 낮으면서 절단시간을 단축할 수 있는 최적의 작동조건은 칼날의 무부하 회전수 630 rpm, 이송속도 0.08 m/s인 것으로 나타났고, 그때의 최대토크는 5.25 kg·cm이었으며 최대소요동력은 33W로 나타났다.

• Food Science and Preservation
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• v.16 no.3
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• pp.412-418
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• 2009

We measured the levels of soluble protein, sugar, free amino acids, minerals, total polyphenols, and flavonoid compounds in Syneilesis palmata as part of a study on nutritional and functional materials for development of valuable foods. The content of soluble protein in the aerial and root portions were 210.36 mg% and 870.42 mg%, respectively. The amount of reducing sugar was 848.12 mg% (aerial parts) and 1,420.91 mg% (roots), and that of free sugar was 14.85 mg% in aerial parts and 355.00 mg% in roots. The free amino acid level in aerial parts was 1,613.10 mg% and that in roots was 3,282.96 mg%. The mineral content of aerial parts was 3,531.53 mg% and that of roots was 1,878.34 mg%. The K, Ca, and Mg levels were greater than those of other minerals, with K comprising more than 75% of the total mineral content. The levels of polyphenol and flavonoid compounds in aerial parts were 1,920.00 mg% and 843.95 mg%, respectively, and those in roots were 487.56 mg% and 91.07 mg%, respectively.