• Journal of the Korean Society of Food Science and Nutrition
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• v.42 no.4
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• pp.534-541
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• 2013

In the present study, the effects of heat treatments on the phenolic components and antioxidative activities of various rice cultivars (from Hwaseongbyeo, Keunnunbyeo, Hongjinjubyeo, and Heugkwangbyeo) were investigated. Each brown rice cultivar was heated at six temperatures (40, 60, 90, 120, 150, and $180^{\circ}C$) for 15 min. The total polyphenolic content (TPC) and total flavonoid content (TFC) of 70% ethanol extracts from heated brown rice were quantified using spectrophotometrical methods, and antioxidant activities determined using DPPH, ABTS radical scavenging activities and reducing power. Hongjinjubyeo had the highest TPC (6.50 mg GAE/g, DB) and ABTS radical scavenging activity (5.85 AAE/g, DB) at $60^{\circ}C$. Also, Heugkwangbyeo showed considerable values for TPC (6.57 and 6.89 mg GAE/g, DB) and ABTS radical scavenging activity (6.29 and 6.11 AAE/g, DB) at $40^{\circ}C$ and $180^{\circ}C$, respectively. Overall, the antioxidant activities of both Hongjinjubyeo and Heugkwangbyeo extracts had a strong positive correlation ($R^2{\geq}0.916$, ${\alpha}$=0.01) with TPC and TFC. These results indicate that heat treatment effectively enhances the antioxidant activity of Hongjinjubyeo and Heugkwangbyeo.

• Korean Chemical Engineering Research
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• v.44 no.1
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• pp.65-72
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• 2006

Recent technical advances in the biorecognition engineering and the microparticle fabrication may enable us to develop the single step purification using magnetic particle, because of its simplicity, efficacy, ease of automation, and process economics. In this study, we used commercial magnetic particles from Seradyn, Inc. (Indianapolis, USA). It was ca. 2.8 micron in diameter, consisted of polystyrene core and magnetite coating, and its surface had carboxyl groups. The model, capture protein was IgG and anti-IgG was used as the ligand molecule. We studied the different surfaces ('nude', ester-activated, and anti-IgG coated) for their biorecognition of IgG. At a high pH condition, we could reduce non-specific binding. Also anti-IgG immobilized magnetic particle could capture IgG more selectively. We attempted 'oriented immobilization' of anti-IgG, in which the polysaccharides moiety near the C-terminus was selectively oxidized and linked to the hydrazine-coated MP, to improve the efficacy of biorecognitive binding. Using this method, the IgG capturing ability was improved by ca. 2 fold. From the binary mixture of the IgG-insulin, IgG could be more selectively captured. In summary, the oriented immobilization of oxidized anti-IgG proved to be as effective as the streptavidin-biotin system and yet simpler and cost-effective. This immobilization method can find its applications in protein biochips and biotargeting.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.4
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• pp.247-256
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• 2013

In this study, alginate beads containing birnessite (Bir-AB), a highly reactive oxidative catalyst for the transformation of phenolic compounds, was prepared and its 1-naphthol (1-NP) removal efficiency was investigated in a batch test. Based on scanning electron microscopy image, it can be inferred that the alginate gel cluster acts as a bridge which bind the birnessite particles together. Kinetic experiment with Bir-AB of different mixing ratios of birnessite to alginate (Bir : AG=0.25 : 1~1 : 1 w/w) indicate that pseudo-first order kinetic constants, $k(hr^{-1})$ for the 1-NP removals increased about 1.5 times when the birnessite mixing ratio was doubled. The removals of 1-NP was found to be dependent on solution pH and the pesudo-first order rate constants were increased from 0.331 $hr^{-1}$ at pH 10 to 0.661 $hr^{-1}$ at pH 4. The analysis of total organic carbon for the reaction solutions showed that a higher removal of dissolved organic carbon was achieved with Bir-AB as compared to birnessite. HPLC chromatographic analysis of the methanol extract after reaction of 1-NP with Bir-AB suggest that the reaction products could be removed through incorporation into the aliginate beads as a bound residue. Mn ions produced from the oxidative transformation of 1-NP by birnessite were also removed by sorption to Bir-AB. The Bir-AB was recovered quantitatively by simple filtration and was reused twice without significant loss of the initial reactivity.

• Journal of Embryo Transfer
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• v.23 no.1
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• pp.43-49
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• 2008

This study was carried out to examine the efficiency of biopsy methods, and the pregnancy rate, calving and abortion rates, gestation length and birth weight of Hanwoo calves following transfer of fresh, frozen and sexed Hanwoo embryos produced in vitro. The survivability of biopsied embryos was 80.0 and 90.0% using aspiration and punching methods at 24 h after culturing, respectively. The ratios of male and female embryos were 42.1 and 52.6%, respectively, and the percentage of sex unidentified was 5.3%. Pregnancy rates was not significantly different between hCG and control group (46.4 vs. 38.5%), fresh and frozen embryos (41.3 vs. 35.0%), and sexed and IVP embryos (27.5 vs. 41.2%) (p>0.05). Calving and abortion rates of IVP and sexed embryos were not significantly different in calving (85.0 vs. 87.0%) and in abortion (15.2 vs. 13.3%) (p<0.05). Gestation length of IVP and sexed calves were 281.3 and 288.2 days in female and 283.0 and 282.3 days in male, and the birth weight of IVP and sexed calves were 23.6 and 25.0 kg in female and 24.6 and 23.8 kg in male, respectively. There were no difference in gestation length and birth weight between IVP embryos and sexed embryos (p>0.05). Administration of hCG to recipients did not improve the pregnancy rate following transfer of Hanwoo embryos produced in vitro and sexed embryos. Although the production of calves derived from sexed Hanweoo embryos cultured in vitro can be obtained, the efficiency of sexed calves production need to be improved in biopsy methods and pregnancy rate. Further study should be focused on the improvement of pregnancy rates for commercial application of embryo transfer.

• Korean Journal of Organic Agriculture
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• v.25 no.2
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• pp.357-371
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• 2017

In this study we evaluated the availability of hairy vetch in citrus (Citrus unshiu Marc.) orchards of volcanic ash soils. The responses to increasing seeding rates and various growing conditions such as altitude, accumulated temperature, and soil chemical properties etc, were analyzed by means of the seedling establishment rate, weed occurrence ratio, and shoot biomass yield of hairy vetch. Field experiments were conducted at five citrus orchards by altitude from Sep. 2015 to Apr. 2016 in Jeju Island, Republic of Korea. Hairy vetch used in the study was 'Cheongpyungbora', developed by National Institute of Crop Science. Seeding rates of hairy vetch consisted of 30, 60 and $90kg{\cdot}ha^{-1}$. Results showed that the seedling establishment rates of hairy vetch were quite similar regardless of seeding rates in all fields and weed occurrence ratio at 30, 60 and $90kg{\cdot}ha^{-1}$ of seeding rates were 11.8, 3.8, and 5.1% (dry wt.), respectively. Both 60 and $90kg{\cdot}ha^{-1}$ of seeding rates, the weed occurrences were decreased by 96.2% and 94.9%. The nitrogen production of hairy vetch at 30, 60 and $90kg{\cdot}ha^{-1}$ of seeding rates were 254, 316, and $315kg{\cdot}ha^{-1}$, respectively. Both 60 and $90kg{\cdot}ha^{-1}$ of seeding rates, The nitrogen production were increased by 24%, compared to $30kg{\cdot}ha^{-1}$ of seeding rate. In these results we were considered that the cost-efficient seeding rate of vetch was $60kg{\cdot}ha^{-1}$ in citrus orchards. Also, this study showed that the shoot biomass of hairy vetch and various cultivative factors were related and The nitrogen production of hairy vetch had a little bit of positive correlation (R=0.2714) with accumulated temperature and considerable correlations with some items (EC ($R=0.4520^{**}$) and exchangeable K ($R=0.4078^{**}$)) of soil chemical properties. Therefore, we were considered that hairy vetch can be used as a leguminous cover crop in citrus orchards, the calculation formula (Y=4.4097X + 33.594 (R=0.9547)) can be suggested for nitrogen yield of hairy vetch by using the shoot fresh weight (X).

• Journal of Animal Science and Technology
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• v.48 no.2
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• pp.211-218
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• 2006

This experiment was conducted to study the effects of the natural deep sea water, which contained approximately 2.3% salt, and various minerals of K, Mg, Ca, Na, Fe, Mn, Zn, Cu etc, on the immune response and antioxidant activity in rats. 24 Sprague Dawley rats were randomly allotted to a control group and 3 treatment groups. Control rats were supplied with filtered tap water, and each treatment group rats were supplied with 0.5% deep sea water, 1% deep sea water and Jijangsoo, respectively, which is upper clear water separated from sediment by the clay. Feed and water were provided ad libitum throughout the experiment that lasted for 4 weeks. The results showed that 1% deep sea water group showed the highest values in weight gain, feed intake, and feed efficiency than those of other groups. The levels of water intake of 1%- and 0.5%-deep sea water, and Jijangsoo group were 49.1%, 22.8%, and 40.5% higher than that of control group, respectively. The Jijangsoo group rats showed that perirenal and epididymal adipose tissue weights were decreased by 32% and 25%(p<0.05), respectively, when compared to control group rats. There were no remarkable differences of serum glucose concentration among all experimental groups. However, insulin concentration of experimental groups were remarkably increased in order of Jijangsoo (4.54), 1% deep sea water (3.70), 0.5% deep sea water (3.25)(p<0.05). B cell and T cell stimulation were increased about 44.7% and 207%, respectively, by 0.5% deep sea water in comparison with control (p<0.05). TBARS values of 0.5 % deep sea water group were significantly lower than that of control(p<0.05). Catalase and SOD activities of 0.5 % deep sea water group were 200% and 47% higher than that of control, respectively. From the results, it can be concluded that the supply of natural deep sea water can slightly improve the physiological activity which modulates immune response and antioxidant activity in rats.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.54 no.1
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• pp.88-103
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• 2009

The volatile and semi-volatile compounds of 5 accessions of domestic scented and 25 accessions of introduced scented were extracted by solid phase microextraction (SPME) and analyzed by gas chromatographymass spectrum (GC-MS). A total of 156 volatile and semivolatile compounds were identified from 30 accessions of aromatic rice, including 32 alcohols, 25 acids, 25 ketones, 21 hydrocarbon, 18 esters, 16 aldehydes, 4 ethers, 5 amines, 2 phenols, 2 bases, and 8 miscellaneous compounds. By UPGMA/Neighbor-join tree analysis, the thirty accessions of aromatic rice could be classified into seven groups according to the major odor or aroma compounds. Group I included indica type of Basmati varieties. Group II and Group IV included japonica type introduced scented. Group III consisted only Hyangmibyeo1ho in domestic scented. Group V and Group VII included indica type of Basmati and non-Basmati varities. Group VI included four of domestic scented of seven accessions excepted Basmati6129, Basmati 6311, and Seratus Malam.

• Economic and Environmental Geology
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• v.35 no.5
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• pp.395-406
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• 2002

The sixty natural springs and community wells used as a drinking water in the Daejeon area are mainly located at the parks and the natural green districts. The purpose of this study is to investigate the characteristics of water quality and the contamination of the springs and the wells, and to suggest the management strategy for the springs and wells. For this study, we undertook water quality data from Daejeon City. According to the statistic analysis of water quality data, unacceptable rate as a drinking water was about 28 percent in 1999 and 24.5 percent in 2000, respectively. Major unacceptable factor is coliform, and others are bacteria, yersinia, color, turbidity, Fe and F. The unacceptable rate shows a roughly positive relationship with precipitation, that is, it shows highest rate during a rainy season between June and September. The major contamination source is likely to be the excrement of wild animals around natural springs and wells. Most of springs are vulnerable to the contamination of coliform and bacteria because of short residence time and shallow circulation in subsurface environment. The water samples collected from 31 springs or wells show weak acidic pHs, the electrical conductivity ranging from 63 to 357 $\mu\textrm{S}$/cm, and the hydrochemical types of Na(Ca)-HC0$_3$ and Ca-HC0$_3$. The groundwater samples of low total dissolved solid(TDS) belong to Na(Ca)-HC0$_3$. type, and the groundwater of high total dissolved solid is shifted towards Ca-HC0$_3$ type in the chemical composition. These hydrochemical characteristics indicate that most natural springs is in the early stage of geochemical evolution. The natural springs should be closed during a rainy season, which shows a high contamination rate. We suggest that a protection barrier around the springs should be built to keep wild animals away from the springs.

• Journal of Korean society of Dental Hygiene
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• v.2 no.2
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• pp.175-186
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• 2002

Fluoride has been one of the most widely studied caries-preventive agents. But the effect of prenatal administration had been controversies for many years. The results showed that there were no influence on reproductive rate of rats with administration of fluoride from 0 to 20 ppm during pregnancy(p>0.05). There was a trend towards slightly increased the mean ash weight in the 1, 5 and 20 ppm groups, as compared with the control group. However, there was no significant differences among groups (p>0.05). The contents of calcium, magnesium and phosphorus in the total bone were increased with the administrated fluoride concentration were increased, but there were no statistically significant differences among groups(p>0.05). The mean fluoride level of 1 ppm group was significantly higher than that of control group, but the concentrations of fluoride in total carcass pups of 5 and 20 ppm groups were significantly less than that of 1 ppm group(p>0.05). The results of this study indicate that the amount of fluoride transferred to the offspring, which may produce anticariogenic effects in the primary teeth of their effects in the primary teeth of their offspring.

• Journal of Animal Science and Technology
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• v.45 no.5
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• pp.767-776
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• 2003

An experiment was conducted to investigate the effects of dietary herbal product(Miracle$^{\circledR}$) supplementation on the performance, nutrient digestibility, fecal microflora and blood parameters in pigs. Forty eight cross bred(Y${\times}$L${\times}$D) weanling pigs (average initial body weight 7.84kg${\pm}$0.17, 28 d old) were used in 35 d feeding trial. Pigs were allotted to 16 raised floor cages(W 35.5cm${\times}$L45cm${\times}$H 55cm). Each treatment had four replications of 3 pigs each. Treatments were: control (T1), CTC 100ppm (T2), Miracle$^{\circledR}$ 0.15% (T3) and CTC 100ppm + Miracle$^{\circledR}$ 0.15% (T4). The herbal product supplementation(T3) tended to improve the weight gain, feed intake, feed/gain but there were no significant differences among treatments. Nutrient digestibility was significantly(P〈0.05) affected by dietary treatments. The pigs fed T3 diet showed the lowest digestibility of DM, crude protein, NFE, P, and amino acids among treatments. Serum cholesterol level of pigs fed T3 diet was lowest and significantly(P〈0.05) lower than those fed antibiotic supplemented diet(T2). Triglyceride and HDL level of the pigs fed T3 diet were also lower than those of others but the differences were not significant. The level of serum IgG was significantly(P〈0.05) different among treatments. The level of serum IgG was highest in T4, followed by T1, T2 and T3. Serum AST(Aspartate aminotransferase) level of the control(T1) was lower than other treatments. Levels of serum total protein, albumin and ALT(Alanine aminotransferase) were not significantly different among treatments. The colony forming units (CFU) of Lactobacillus, Cl. perfringens and E.coli in feces were not significantly different among treatments but those of Cl. perfringens of T2 (CTC 100ppm) showed the lowest count at both 3rd and 5th wk. It is concluded that herbal product Miracle$^{\circledR}$ does not significantly affect growth performance of weanling pigs but it influenced the digestibility, serum IgG, cholesterol and AST level. The combination of the herbal product Miracle$^{\circledR}$ with antibiotic(CTC) showed no synergistic effects.