Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Production of Calves Following Transfer of Sexed Hanwoo Embryos and Hanwoo Embryos Cultured In Vitro

한우 체외 수정란 및 성 감별 수정란 이식에 의한 송아지 생산

  • Min, Chan-Sik (Gyengsangnamdo Agriculture Research & Extension Services) ;
  • Song, Sang-Hyun (Institute of Agriculture & Life Sciences, Gyeongsang National University) ;
  • Song, Gwi-Dong (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Chung, Woo-Jae (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Rho, Chi-Won (Gyengsangnamdo Agriculture Research & Extension Services) ;
  • Kang, Yang-Soo (Gyengsangnamdo Agriculture Research & Extension Services) ;
  • Park, Choong-Saeng (Division of Applied Life Science (BK21), Graduate School of Gyeongsang National University) ;
  • Kong, Il-Keun (Institute of Agriculture & Life Sciences, Gyeongsang National University)
  • 민찬식 (경상남도 농업기술원) ;
  • 송상현 (경상대학교 농업생명과학연구원) ;
  • 손귀동 (경상대학교 응용생명과학부) ;
  • 정우재 (경상대학교 응용생명과학부) ;
  • 노치원 (경상남도 농업기술원) ;
  • 강양수 (경상남도 농업기술원) ;
  • 박충생 (경상대학교 응용생명과학부) ;
  • 공일근 (경상대학교 농업생명과학연구원)
  • Published : 2008.03.31
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Abstract

This study was carried out to examine the efficiency of biopsy methods, and the pregnancy rate, calving and abortion rates, gestation length and birth weight of Hanwoo calves following transfer of fresh, frozen and sexed Hanwoo embryos produced in vitro. The survivability of biopsied embryos was 80.0 and 90.0% using aspiration and punching methods at 24 h after culturing, respectively. The ratios of male and female embryos were 42.1 and 52.6%, respectively, and the percentage of sex unidentified was 5.3%. Pregnancy rates was not significantly different between hCG and control group (46.4 vs. 38.5%), fresh and frozen embryos (41.3 vs. 35.0%), and sexed and IVP embryos (27.5 vs. 41.2%) (p>0.05). Calving and abortion rates of IVP and sexed embryos were not significantly different in calving (85.0 vs. 87.0%) and in abortion (15.2 vs. 13.3%) (p<0.05). Gestation length of IVP and sexed calves were 281.3 and 288.2 days in female and 283.0 and 282.3 days in male, and the birth weight of IVP and sexed calves were 23.6 and 25.0 kg in female and 24.6 and 23.8 kg in male, respectively. There were no difference in gestation length and birth weight between IVP embryos and sexed embryos (p>0.05). Administration of hCG to recipients did not improve the pregnancy rate following transfer of Hanwoo embryos produced in vitro and sexed embryos. Although the production of calves derived from sexed Hanweoo embryos cultured in vitro can be obtained, the efficiency of sexed calves production need to be improved in biopsy methods and pregnancy rate. Further study should be focused on the improvement of pregnancy rates for commercial application of embryo transfer.

본 연구는 한우 체외 수정란의 성 감별과 신선란, 동결란 및 성 감별 수정란을 이식한 후 수태율, 분만율과 유산율, 생시 체중, 임신 기간을 조사하기 위하여 수행하였다 Aspiration과 punching법으로 biopsied한 수정을 24시간 배양 후 생존율은 각각 80.0%와 90.0%로 유의적인(p>0.05) 차이는 없었다. 수정란을 성 감별한 결과, 웅성 수정란과 자성 수정란의 비율은 각각 42.1%와 52.6%였으며, 5.3%는 수정란의 성을 판정하지 못하였다. hCG를 처리한 수란우의 수태율은 46.4%로서 무처리구(38.5%)에 비하여 높은 경향이었으나, 처리구간의 유의적인 차이는 없었다. 신선란과 동결란을 수정란 이식 후 수태율은 각각 41.3와 35.0%로서 유의적인 차이는 없었다. 성 감별된 수정란과 성 감별하지 않은 체외 수정란 이식 후 수태율은 각각 27.5와 42.1%로 유의적인 차이는 나타나지 않았다. 성 감별 된 수정란과 성 감별하지 않은 체외 수정란 유래 송아지의 분만율은 각각 85.0와 87.0%이었으며, 유산율은 각각 15.2와 13.3%로서 분만율과 유산율은 유의적인 차이는 없었다. 성 감별된 수정란과 성 감별하지 않은 체외 수정란 유래 송아지의 임신 기간은 각각 281.3일(female), 283.0일(male)과 288.2일(female), 282.3일(male)이었으며, 생시 체중은 각각 23.6(female), 24.6(남)과 25.0(female), 23.8 kg(male)로 유의적인 차이는 없었다. hCG 처리한 수란우는 한우 수정란 이식 후 수태율 개선 효과는 있는 것으로 판단된다. 성 감별 수정란 이식에 의해 송아지는 생산되었으나, 수정란의 biopsy 방법, 수정란 이식 후 수태율 개선과 같은 송아지 생산 효율을 개선해야 할 것으로 사료된다. 수정란 이식의 산업화가 정착하기 위해서는 동결란과 신선란 이식 후 수태율의 개선에 대한 노력이 필요할 것으로 사료된다.

Keywords

References

  1. Agca Y, Monson RL, Northry DL, Preschel DE, Schaefer DM and Rutledge JJ. 1998. Normal calves from transfer of biopsied sexed and vitrified IVP bovine embryos. Theriogenology 50:129-145 https://doi.org/10.1016/S0093-691X(98)00120-4
  2. Avery B, Madison V and Greve T. 1991. Sex and development in bovine in vitro fertilized embryos. Theriogenology 35: 953-963 https://doi.org/10.1016/0093-691X(91)90306-X
  3. Breadback K and Bredback P. 1996. Glucose controls sex related growth rate differences of bovine embryos produced in vitro. J. Reprod. Fertil. 106:169-172 https://doi.org/10.1530/jrf.0.1060169
  4. Broadbent PJ, Stewart M and Dolman DF. 1991. Recipient management and embryo transfer. Theriogenology 35:125- 139 https://doi.org/10.1016/0093-691X(91)90152-4
  5. Callesen H, Bak A and Greve T. 1994. Embryo recipients: dairy cow or heifer? Proc. of the 10th Scientific Meeting of the AETE, Lyon, France, pp. 125-135
  6. Chagas SJ and Lopes LC. 2005. Luteotrophic influence of early bovine embryos and the relationship between plasma progesterone concentrations and embryo survival. Theeriogenology 64:49-60 https://doi.org/10.1016/j.theriogenology.2004.10.019
  7. Chatzimeletiou K, Morrison EE, Panagiotidis Y, Prapas N, Parapas Y, Rutherford AJ, Grudinskas G and Handyside AH. 2005. Comparison effects of zona drilling by non-contact infrared laser or acis Tyrode's on the development of human biopsied embryos as reavealed by blastomere viability, cytoskeletal analysis and molecular cytogenetics. Reprod. Biomed. 11:697-710 https://doi.org/10.1016/S1472-6483(10)61688-4
  8. Chrenek P, Boulanger L, Heyman Y, Uhrin P, Laurinicki J, Bulla J and Renard JP. 2001. Sexing and multiple genotype analysis from a single cell of bovine embryo. Theriogenology 55:1071-1081 https://doi.org/10.1016/S0093-691X(01)00467-8
  9. Dochi O, Yamamoto Y, Saga H, Yoshida N, Kano N, Maeda J, Miyata K, Yamaguchi A, Tominaga K, Oda Y, Nakashima T and Inohae S. 1998. Direct transfer of bovine embryos frozen-thawed in the presence of polyethylene glycol or ethylene glycol under on-farm conditions in an integrated embryo transfer program. Theriogenology 49:1051-1058 https://doi.org/10.1016/S0093-691X(98)00053-3
  10. Gordon JC, Aguera S and Castejon F. 2004. Sexing in vitro produced bovine embryos, at different stages of development, using rat H-Y antiserum. Theriogenology 62:35-43 https://doi.org/10.1016/j.theriogenology.2003.05.007
  11. Hasler JF, Henderson WB, Hurtgen PJ, Jin ZQ, McCauley AD, Mower SA, Neely B, Dhuey LS, Stokes JE and Trimmer SA. 1995. Production, freezing and transfer of bovine IVF embryos and subsequent calving results. Theriogenology 43:141-152 https://doi.org/10.1016/0093-691X(94)00020-U
  12. Hasler JF. 2001. Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle. Theriogenology 56:1401-1415 https://doi.org/10.1016/S0093-691X(01)00643-4
  13. Kruip TAM and den Dass JHG. 1997. In vitro produced and cloned embryos: effects on pregnancy, parturition and offspring. Theriogenology 47:43-52 https://doi.org/10.1016/S0093-691X(96)00338-X
  14. Lee JH, Park JH, Lee SH, Park CS and Jin DI. 2004. Sexing single blastomere derived from IVF bovine embryos by fluorescence in situ hybridization (FISH). Theriogenology 62: 1452-1458 https://doi.org/10.1016/j.theriogenology.2004.02.012
  15. Lopes RF, Forell F, Oliveira AT and Rodrigues JL. 2001. Splitting and biopsy for bovine embryo sexing under field conditions. Theriogenology 56:1383-1392 https://doi.org/10.1016/S0093-691X(01)00641-0
  16. Massip A, Mermillod P and Dinneys A. 1995. Morphology and biochemistry of in vitro produced bovine embryos: implications for their cryopreservation. Hum. Reprod. 10:3004- 3011 https://doi.org/10.1093/oxfordjournals.humrep.a135837
  17. McEvoy JD, Mayne CS and McCaughty WJ. 1995. Production of twin calves with in vitro fertilised embryos: Effects on the reproductive performance of dairy cows. Vet. Rec. 136: 627-632 https://doi.org/10.1136/vr.136.25.627
  18. Nishigai M, Kamomae H, Tanaka T and Kaneda Y. 1998. Pregnancy rate and blood progesterone concentrations on the previous day and the day of frozen embryo transfer in parous recipient cows of Japanese Black. J. Reprod. Dev. 44:413-419 https://doi.org/10.1262/jrd.44.413
  19. Nishigai M, Kamomae H, Tanaka T and Kaneda Y. 2000. The relationship of blood progesterone and estrogen concentrations on the day before and the day of frozen-thawed embryo transfer to pregnancy rate in Japanese Black cattle. J. Reprod. Dev. 46:235-243 https://doi.org/10.1262/jrd.46.235
  20. Nishigai M, Kamomae H, Tanaka T and Kaneda Y. 2001. The effect of administration of human chronic gonadotropin in enhancing bovine corpus lutea luteinization and luteal function. J. Reprod. Dev. 47:283-294 https://doi.org/10.1262/jrd.47.283
  21. Nishigai M, Kamomae H, Tanaka T and Kaneda Y. 2002. Improvement of pregnancy rate in Japanese Black cows by administration of hCG to recipients of transferred frozenthawed embryos. Theriogenology 58:1597-1606 https://doi.org/10.1016/S0093-691X(02)01062-2
  22. O'Kearney-Flynn M, Wade M, Duffy P, Gath V, Boland MP and Dorbinsky JR. 1998. Effect of cryopreservation on IVP cattle embryo development in vitro and in vivo. Theriogenology 49:178 (Abstract) https://doi.org/10.1016/S0093-691X(98)90531-3
  23. Park HJ, Lee JH, Choi KM, Joung SY, Kim JY, Chung GM, Jin DI and Im KS. 2001. Rapid sexing of preimplantation bovine embryo using consecutive and multiplex polymerase chain reaction (PCR) with biopsied single blastomere. Theriogenology 55:1843-1853 https://doi.org/10.1016/S0093-691X(01)00526-X
  24. Sakaguchi M, Geshi M, Hamano S, Yona M and Nagai T. 2002. Emrbyonic calving losses in bovine mixed-breed twins induced by transfer of in vitro produced embryos to bred recipients. Anim. Reprod. Sci. 72:209-221 https://doi.org/10.1016/S0378-4320(02)00090-8
  25. Santos JE, Thatcher WW, Pool L and Overton MW. 2001. Effect of human chronic gonadotropin on luteal fuction and reproductive performance of high-producing lactating Holstein dairy cows. J. Anim. Sci. 79:2881-2894
  26. Shea BF. 1999. Determining the sex of bovine embryos using polymerase chain reaction results: a six year retrospective study. Theriogenology 51:841-854 https://doi.org/10.1016/S0093-691X(99)00030-8
  27. Smith AK and Grimmer SP. 2002. Pregnancy rates for grade 2 embryos following administration of synthetic GnRH at the time of transfer in embryo-recipient cattle. Theriogenology 57:2083-91 https://doi.org/10.1016/S0093-691X(02)00704-5
  28. van Vleck LD, Pollak JE and Brandford-Oltenacu EA. 1987. Genetics for Animal Science. New York: Freeman WH. 287- 313
  29. 김용준, 이창민, 정구남, 이해리, 조성우, 김용수, 신동수, 홍유미, 유일정. 2003. 성 감별된 한우 체외 수정란 이식. 한국수정란이식학회지 18:97-108
  30. 김용준, 정구남, 이해이, 조성우, 김용수, 유일정. 2000. 한우 체외 수정란 Biopsy 후 PCR 기법을 이용한 성 판정과 성 감별 수정란의 이식. 한국수정란이식학회지 15:219-230
  31. 손동수, 조상래, 최창용, 최선호, 한만희, 김현종, 조창연, 진현주, 김영근, 정연길, N. Saito, S. Kageyama, 최상용. 2005. 한우 체내수정란의 성판별 후 이식으로 우수 송아지 생산. 한국수정란이식학회지 20:163-168
  32. 조상래, 최선호, 김현종, 한만희, 최창용, 정연길, 손동수. 2005. LAMP방법에 의한 소 수정란의 성 판별과 biopsy에 따른 수정란의 발달. 한국수정란이식학회지 20:169-176