Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Factors Affecting on Pregnancy Rate of Recipients Following Transfer of Hanwoo Embryos Produced In Vivo

한우 체내 수정란 이식 후 수태율에 미치는 요인

  • Son, Gwi-Dong (Division of Applied Life Science(BK21), College of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Song, Sang-Hyun (Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Jeong, Woo-Jae (Division of Applied Life Science(BK21), College of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Park, Choong-Saeng (Division of Applied Life Science(BK21), College of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Lee, Jung-Gyu (Division of Applied Life Science(BK21), College of Agriculture & Life Science, Graduate School of Gyeongsang National University) ;
  • Kong, Il-Keun (Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • 손귀동 (경상대학교 농업생명과학대학 응용생명공학부) ;
  • 송상현 (경상대학교 농생명과학원) ;
  • 정우재 (경상대학교 농업생명과학대학 응용생명공학부) ;
  • 박충생 (경상대학교 농업생명과학대학 응용생명공학부) ;
  • 이정규 (경상대학교 농업생명과학대학 응용생명공학부) ;
  • 공일근 (경상대학교 농생명과학원)
  • Published : 2008.03.31
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Abstract

This study was performed to improve the pregnancy rates of recipients following transfer of bovine embryos produced in vivo. Superovulation response didn't showe significant differences between each season (4.18 in spring; 4.36 in summer; 5.50 in fall; 4.38 in winter). Pregnancy rate was significantly different (p<0.05) between fresh (43.4%) and frozen embryos (17.2%). In administration of hCG to recipients, the pregnancy rate of fresh embryos (45.7%) was slightly higher than that of control (35.3%), but the pregnancy rates of frozen embryos in control group (25.0%) was higher than that of hCG group (16.0%). When synchrony of recipient and embryo was -2, -1, 0 and 1, the pregnancy rates were 20.0, 45.0, 30.3 and 26.3%, respectively. The pregnancy rates of recipients synchronized by naturally or $PGF_{2{\alpha}}$, CIDR/$PGF_{2{\alpha}}$ and E/P/CIDR/$PGF_{2{\alpha}}$/E treatments were 35.3, 48.0, 29.0 and 40.0%, respectively. Gestation lengths and birth weights of female and male calf were 288 and 290.5 days, 28.3 and 30.0 kg, respectively. The results were showed that the superovulation response was not affected by seasons, and also pregnancy rate didn't increase by administration of hCG, synchrony of embryo and recipients, synchrony methods. Further study and concern should be focused on improving the embryo freezing and pregnancy rate for commercial embryo transfer.

발정 주기와 상관없이 CIDR를 삽입하는 날에 2.5 mg estradiol benzoate, 50 mg progesterone을 주사하였다. CIDR 삽입 후 4일 동안 FSH를 감량법으로 12시간 간격으로 주사하였으며, FSH 주사 5, 6회째에 $PGF_{2{\alpha}}$를 투여했다. 1회째 $PGF_{2{\alpha}}$ 주사 24시 간 후 CIDR를 제거하고 GnRH를 주사하였다. 공란 우들을 1번째 $PGF_{2{\alpha}}$ 주사 후 60시간과 72시간에 수정을 시켰다. 인공수정 7일 후 회수된 수정란을 수정란 이식 때 prosesterone 농도를 증가시켜 수태율을 향상시키기 위해 수란우에 hCG 1,500 IU를 주사하였다. 수란우의 발정 동기화는 (1) 자연 발정우(natural), (2) 직장 검사로 황체가 존재하는 수란우에 25 mg $PGF_{2{\alpha}}$ 처리구($PGF_{2{\alpha}}$), (3) CIDR를 질내에 7일간 삽입하고 제거하는 당일 $PGF_{2{\alpha}}$ 25 mg을 투여하는 방법(CIDR + PG법) 및 (4) CIDR를 삽입하고 2.5 mg estradiol benzoate, 50 mg progesterone 주사 후 7일 후 제거하는 당일 $PGF_{2{\alpha}}$ 25 mg 투여 후 뒷날 estradiol benzoate 투여(E/P/CIDR/$PGF_{2{\alpha}}$/E) 방법으로 발정을 유도하였다. 계절에 따른 과배란 반응은 회수된 수정란들은 계절간에 유의적 차이가 보여주지 못하였다(봄; 4.18, 여름; 4.36, 가을; 5.50, 겨울 4.38). 신선란(43.4%) 이식 후 수태율은 동결란(17.2%)보다 높게 나타났다. 신선란을 이식하여 hCG 처리한 한우 수란우의 수태율(45.7%)은 대조구(35.3%)보다 약간 높게 나왔다. 그러나 동결란을 수란우에 이식하였을 때, 대조구의 수태율(25.0%)이 hCG 처리구(16.0%)보다 높게 나타났다. 수정란 이식 후 -2, -1, 0 및 1일째의 수란우와 수정란의 동기화 일에 따른 수태율은 20.0, 54.0, 30.3% 그리고 26.3%였다. 자연 발정 수란우, $PGF_{2{\alpha}}$, CIDR/ $PGF_{2{\alpha}}$, E/P/CIDR/ $PGF_{2{\alpha}}$/E로 발정된 수란우들의 수태율은 각각 35.3, 48.0, 29.0 및 40.0%로 나타났다. 하지만 이들 동기화 방법에 따른 유의차는 없었다. 수정란 이식 후 태어난 수송아지와 암 숫송아지의 임신 기간은 각각 288일과 290.5일이었으며, 생시 체중은 각각 28.3과 30.0 kg이었다. 결론적으로 계절에 따른 과배란 반응의 변동은 없었고, hCG 처리, 수란우와 수정란의 동기화, 동기화 방법에 따른 수태율의 향상을 보여주지 못하였다. 향후에는 수정란 이식의 산업화를 위해서는 수정란의 동결과 수태율 향상에 관심을 가져야 할 것이다.

Keywords

References

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