• Reproductive and Developmental Biology
• /
• v.30 no.1
• /
• pp.21-26
• /
• 2006

This study was examined the effects of concentrations of polyvinylpyrrolidone(PVP) and supplementation of EGF, cysteine and PVP during in vitro maturation on the development of bovine embryos. In experiment 1, 0.1 to 3.0% PVP was supplemented to IVM medium before IVF. The development rates to the blastocyst stage was significantly higher in 0.5% PVP group than 3.0% PVP group (P<0.05). In experiment 2, EGF, rysteine and PVP were supplemented to IVM medium. The hight cleavage rate was obtained from cysteine group, but blastocyst formation rates did not differ among groups. The highest total cell number and inner cell mass (ICM) cell number were observed in cysteine group. In PVP group, ICM cell number was significantly low than those of cysteine and control groups (P<0.05). After embryo transfer, pregnancy rate was significantly low in PVP group compared to other groups (P<0.05). These results indicate that the supplementation of PVP in IVM medium support the embryo development, but has a deteriorate effect on the blastocyst quality.

• The Journal of Korean Institute of Communications and Information Sciences
• /
• v.22 no.9
• /
• pp.1998-2004
• /
• 1997

In this paper, we have analyzed the bit error performance ofthe chip-spreading OCDM system proposed in the previous papaer and compared the results, through computer simulations, with those of conventional DS/CDMA system which employs the maximal ration combining method. WE have shown, analytically, that the BER upper bound of the OCDM system coincides with that of the conventional DS/CDMA system and the lower bound exhibits much improved results. From the simulation results, we have confirmed that the BER curves of proposed OCDM system actually lie between the two theoretical bounds and its performance is superior to that of the DS/CDMA system.

• Korean Journal of Animal Reproduction
• /
• v.26 no.1
• /
• pp.79-85
• /
• 2002

We investigated the optimal concentration and exposure time of cycloheximide(CHX) on development of activated porcine oocytes following electrical pulse(EP). After 42~44 h maturation, oocytes were treated with 0.1% hyaluronidase, and denuded cumulus cells by pipetting. Oocytes were stimulated by electric pulse (1.2 kV/cm, 30 $\mu$sec, 1 pulse) or incubated for 3, 5 and 7 h in cycloheximide (1, 5 and 10 $\mu\textrm{g}$/$m\ell$, respectively) following electric pulse, and cultured for 8 days. Cleavage rate of oocytes activated with 10 $\mu\textrm{g}$/$m\ell$ CHX following EP was significantly (P＜0.05) higher than those of 1 $\mu\textrm{g}$/$m\ell$ (86.8% vs. 74.4%). The developmental competence of oocytes incubated to 5 $\mu\textrm{g}$/$m\ell$ of CHX was significantly (P＜0.05) higher development to blastocysts (13.3%), compared with 10 $\mu\textrm{g}$/$m\ell$ of CHX (5.6%). When the oocytes were activated with 5$\mu\textrm{g}$/$m\ell$ CHX for 3, 5, and 7 h following EP, the cleavage rate of oocytes in 5 h group(86.6%) was significantly (P＜0.05) higher than that in 3 h group(73.2%). The developmental rate of oocytes to morula in 5 and 7 h groups(26.7% and 16.4%) were significantly (P＜0.05) high than that in 3 h group(14.5%). Matured oocytes were activated with electric pulse (EP) or electric pulse combined with cycloheximide (EP + CHX) and cultured for 8 days. The rate of cleavage and development to blastocyst (80.1% and 11.6%) of activated with EP group were similar to EP combined with CHX group. When activated with EP or EP combined with CHX, the mean cell number of blastocysts were less in the activated with EP (18.67$\pm$5.53) than in the activated EP combined CHX (20.71$\pm$6.16), but not significantly different. This results suggest that, when the porcine oocytes were activated with CHX following EP, the developmental rate of activated oocytes can be improved by treated with a concentration of 5 $\mu\textrm{g}$/$m\ell$ CHX for 5 h exposure time.

• Journal of Embryo Transfer
• /
• v.22 no.2
• /
• pp.121-126
• /
• 2007

The objective of this study was carried out to examine the selection effects of in vitro matured porcine follicular oocytes with polar body extrusion and early cleavage as non-invasive marker to know the developmental competence in advance. The porcine oocytes matured for 48 h were examined the polar body extrusion. The examined oocytes were matured for additional $16{\sim}18h$ and activated with 7% ethanol and cultured in $5{\mu}g/ml$ cytochalasin B for 5 h for diploid formation. The treated oocytes were cultured and examined the cleavage after 48 h and continued culturing for 5 days. The oocytes of 21.9% were discarded in morphological selection and 32.1% oocytes were discarded by failure of first polar body extrusion. The selected oocytes were matured and activated and then after 48 h the cleavage rates were examined. In morphologically selected oocytes, 15.8% oocytes were not cleaved and 52.6% oocytes were normally cleaved and 31.6% oocytes were hyper-cleaved over 8-cell stage. However in the first polar body extruded oocytes, 7.1% oocytes were not cleaved and 73.1% oocytes were normally cleaved and 19.8% oocytes were hyper-cleaved. The morphologically selected embryos that not cleavage-selected were developed in 16.7% up to blastocyst and the morphologically selected and cleavage-selected embryos were developed in 31.7%. The polar body extruded oocytes that were not carried out cleavage selection were developed in 39.0% and the polar body extruded and cleavage-selected embryos were developed 49.0%. The first cleavage timing was examined with 12 h interval after activation. In $0{\sim}12,\;12{\sim}24,\;24{\sim}36,\;and\;36{\sim}48h$ intervals, 4.1%, 68.6%, 19.1%, and 2.3% oocytes were cleaved and 5.9% oocytes were not cleaved until 48 after activation. The cleaved oocytes in each interval were cultured and developed upto blastocyst with 0, 39.1, 9.5, and 0%, respectively. This results suggests that polar body extruded and cleaved at $12{\sim}36h$ embryo has higher developmental potential than the others.

• The Journal of the Acoustical Society of Korea
• /
• v.13 no.4
• /
• pp.5-11
• /
• 1994

In this paper, the ciassification of Korean character type using multi neural network and fuzzy inference based on block partition is studied. For the effective classification of a consonant and a vowel, block partition method which devide the region of a consonant and a vowel for each type in the character is proposed. And the partitioned block can be changed according to the each type adaptively. For the improvement of classification rate, the multi neural network with a whole and a part neural network is consisted, and the character type by using fuzzy inference is decided. To verify the validity of the proposed method, computer simulation is accomplished, and from the classification rate $92.6\%$, the effectivity of the method is confirmed.

• Journal of KIISE:Computer Systems and Theory
• /
• v.32 no.5
• /
• pp.187-195
• /
• 2005

A new segmentation-based image coding method which no needs transmission of contour data is proposed. The shape information acts as bottleneck in the segmentation-based video coding because it has much portion of transmission data. The proposed method segments a previous decoded frame, instead of a current frame. As a result, there is no need for transmission of contour information to a decoder. Therefore, the saved bits can be assigned to encode other information such as error signals. As shown in experiment results, if data rate is very highly increased due to abrupt motion under very low bit rate coding having limited transmission bits, PSNR of conventional block-based method go down about 20dB, while the proposed method shows a good reconstruction quality without rapid PSNR drop.

• Journal of Broadcast Engineering
• /
• v.27 no.3
• /
• pp.361-368
• /
• 2022

Quaternary tree plus multi-type tree (QT+MTT) structure was adopted in the Versatile Video Coding (VVC) standard as a block partitioning tool. QT+MTT provides excellent coding gain; however, it has huge encoding complexity due to the flexibility of the binary tree (BT) and ternary tree (TT) splits. This paper proposes a fast inter coding unit (CU) partitioning algorithm for BT and TT split types based on prediction accuracy functions using the mean of the absolute error (MAE). The MAE-based decision model was established to achieve a consistent time-saving encoding with stable coding loss for a practical low complexity VVC encoder. Experimental results under random access test configuration showed that the proposed algorithm achieved the encoding time saving from 24.0% to 31.7% with increasing luminance Bjontegaard delta (BD) rate from 1.0% to 2.1%.

• Current Research on Agriculture and Life Sciences
• /
• v.6
• /
• pp.145-156
• /
• 1988

This study was conducted to investigate the effect of embryo stage on bisection rates of embryos, on development of separated demi-embryos and on subsequent development to full term following transfer of demi-embryos to recipients. The results obtained in this study are summarized as follows. The 2-, 4-, 8-cell and morula embryos were mostly obtained at 36-42, 48-54, 62-66 and 72-78 hours after injection of hCG, respectively, and the average number of embryos recovered per head was 17.0. The bisection rate of 8-cell embryos was 83.6%, which was significantly higher than that of morula embryos, 65.5%. But the development of morula demi-embryos to blastocyst after in-vitro culture was better than that of 8-cell demi-embryos and the rate was 76.5% and 60.9%, respectively. When the 2, 10 and 15 cultured demi-embryos or intact embryos were transferred to each recipient mouse, respectively. the highest pregnancy rate was obtained when 15 embryos were transferred. And the overall offspring production rate of intact embryos was higher than that of demi-embryos.

• Journal of Embryo Transfer
• /
• v.18 no.1
• /
• pp.27-33
• /
• 2003

The study was carried out to investigate the effects of morphology, reproductive cycle, incubation time and activation of oocytes in vitro maturation of canine oocytes and development of canine IVM/IVF embryos. The results were summarized as follows: 1. The developmental rates to 16 cells of fresh, salts and 4$^{\circ}C$-stored oocytes with and without cumulus cells were 14.3%, 5.0% and 7.5%, 2.8% and 5.7%, 0.0%, respectively. The rate of oocytes with cumulus cells(5.7%~14.3%) was higher than that of denuded oocytes(0.0%~5.0%). 2. The developmental rate to If cells of in vitro cultured oocytes recovered from ovaries collected at different stages of the reproductive cycle were 0.0%, 10.7%, 1.5%, respectively. 3. The developmental rate to 16 cells of fresh oocytes with cumulus cell cultured for 24, 32 and 48 hrs in $CO_2$ incubator were 0.0%, 5.3%, 11.8%, respectively. The rate of oocytes cultured for 48 hrs was higher than that oocytes cultured for 24 and 32 hrs. 4. The development to If cells treated activation and non-activation oocytes were 15.0%, 6.7%, respectively. The rate of oocytes treated activation was higher than that oocyte treat non-activation.

• Journal of the Institute of Electronics Engineers of Korea SP
• /
• v.41 no.3
• /
• pp.101-109
• /
• 2004

In this paper, we propose an efficient algerian for wavelet-based sti image coding method that utilizes the rate-distortion (R-D) theory. Since conventional tree-structured image coding schemes do not consider the rate-distortion theory properly, they show reduced coding performance. In this paper, we apply an rate-distortion optimized embedding (RDE) operation into the set partitioning in hierarchical trees (SPIHT) algorithm. In this algorithm, we use the rate-distortion slope as a criterion for the coding order of wavelet coefficients in SPIHT lists. We also describe modified set partitioning and rate-distortion optimized list scan methods. Experimental results demonstrate that the proposed method outperforms the SPIHT algorithm and the rate-distortion optimized embedding algerian with respect to the PSNR (peak signal-to-noise ratio) performance.