• Title/Summary/Keyword: 분자 유전학

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Complete genome sequence of Pantoea intestinalis SRCM103226, a microbial C40 carotenoid zeaxanthin producer (식용곤충 갈색거저리에서 분리한 카로테노이드 생성균주인 Pantoea intestinalis SRCM103226 균주의 유전체 해독)

  • Kim, Jin Won;Ha, Gwangsu;Jeong, Seong-Yeop;Jeong, Do-Youn
    • Korean Journal of Microbiology
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    • v.55 no.2
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    • pp.167-170
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    • 2019
  • Pantoea intestinalis SRCM103226, isolated from edible insect mealworm overproduces zeaxanthin as a main carotenoid. The complete genome of P. intestinalis SRCM103226 was sequenced using the Pacific Biosciences (PacBio) RS II platform. The genome of P. intestinalis SRCM103226 comprises a 4,784,919 bp circular chromosome (53.41% G+C content), and is devoid of any extrachromosomal plasmids. Annotation using the RAST server reveals 4,332 coding sequences and 107 RNAs (22 rRNA genes, 85 tRNA genes). Genome annotation analysis revealed that it has five genes involved in the carotenoid pathway. The genome information provides fundamental knowledge for comparative genomics studies of the zeaxanthin pathway.

한국산 초파리 집단의 유전 생화학적 연구: 노랑 초파리의 $\alpha$-Glycerophosphate dehydrogenase allele에 대하여

  • 정용재;한영수;정영란
    • The Korean Journal of Zoology
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    • v.25 no.3
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    • pp.123-129
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    • 1982
  • In order to establish a biochemical genetic system in Drosophila populations in Korea, the $\\alpha$-glycerophosphate dehydrogenase alleles of eleven natural populations of D. melanogaster in Korea were examined by means of agarose gel electrophoresis. The results obtained are presented below: 1. $\\alpha$-Glycerophosphate dehydrogenase ($\\alpha$-GPDH) allele is scored for eleven natural populations of D. melanogaster in Korea, resulting that $\\alpha$-GPDH is found to be widely polymorphic for two electrophoretic variants. 2. The heterozygosity of $\\alpha$-GPDH is calculated as $40\\sim50%$. 3. The frequency of the FF genotype of $\\alpha$-GPDH is found to be roughly same as the SS genotype, but less than the FS genotype. 4. The F gene of $\\alpha$-GPDH is distributed almost frequently as the S gene.

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Variation of Dielectric Constant of Sand due to Water Content and Measuring Frequency (수분함량과 측정주파수에 따른 사질토 지반의 유전상수 변화)

  • 이주형;오명학;박준범;김형석
    • Journal of the Korean Geotechnical Society
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    • v.18 no.6
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    • pp.129-139
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    • 2002
  • Dielectric constant measurement has drawn much attention in the investigation of the properties and contaminations of subsurface. In this study, by varying the frequency from 75 kHz to 12 MHz, dielectric constant was measured for the weathered granite soil and Jumunjin sand having different water contents and dry density. The dielectric constant of sand showed the dispersive behavior indicating that dielectric constant decreased with frequency of an electric field. And the dielectric constant of soil increased as water content and/or dry density increased due to the decrease of air portion and/or the increase of amount of water molecules which could contribute to the development of orientation polarization. The dielectric constant of sand showed a linear relationship with the moisture density, considering both water content and dry density. At low frequency, the dielectric constants calculated by Maxwell's, Topp's and CRIM equation deviated from measured values. It could be explained by the fact that those equations did not consider dispersive behavior of dielectric constant with the frequency.

Geographic variation of Grey-capped Greenfinch (Chloris sinica) in Korea (한국에서 방울새(Grey-capped Greenfinch, Chloris sinica)의 지리적 변이에 대한 연구)

  • Park, Jong-Gil;Kim, Joo-Eun;Jin, Kyoung-Soon;Park, Chungoo;Nam, Dong-Ha
    • Korean Journal of Ornithology
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    • v.25 no.2
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    • pp.117-125
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    • 2018
  • The Grey-capped greenfinch (Chloris sinica) is a polytypic species that exhibits complicated geographical variation in morphology. This study provides an intraspecific phylogeographic variability of C. sinica populations in Korea with their morphometric data. The observed morphometric variations were that Ulleung island population was morphologically distinct in bill length and depths as compared to the mainland populations. Phylogenetic relationships among mitochondrial COX1 regions provided evidence for genetic differentiation between Ulleung and mainland populations. However, their genetic distances and nucleotide diversities were very low, highlighting their recent divergence. The needs for additional research is heightened to substantiate if the genetic clines in different localities may arise in C. sinica subspecies, each of which could have different breeding and wintering habitats, distribution patterns, and migration pathways.

Utilization of Porcine Clotted and Dried Blood for Estrogen Receptor Gene PCR-RFLP (에스트로겐 수용체 유전자의 다형 현상 추정을 위한 응고 및 건조된 돼지 혈액의 이용)

  • Seo, D.S.;Yang, S.H.;Park, H.B.;Park, S.S.;Hong, K.C.;Ko, Y.
    • Korean Journal of Animal Reproduction
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    • v.23 no.2
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    • pp.159-163
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    • 1999
  • Recent development of the molecular biology techniques has made it possible to characterize and analyze early diagnoses of genetic disorders and economic trait loci. In this study, porcine genomic DNA was extracted from both clotted and dried blood to analyze the porcine estrogen receptor (ER) gene by polymerase chain reaction (PCR). By the methods reported here, genomic DNA extracted from clotted or dried blood was efficient enough to detect ER gene by PCR. Moreover, the PCR-RFLP (restriction fragment length polymorphism) of ER gene was identified by PvuII restriction enzyme. Thus the results obtained from this study show that the clotted and dried blood was useful for identification of the certain genotype in a rapid manner with low cost. Importantly, this study implies that the whole blood can be economically utilized in studies of endocrinology, molecular biology, and genetics by obtaining both serum and DNA simultaneously in an efficient manner.

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Decrease in Genetic Variation of Overwintering Populations of the Diamondback Moth during Seasonal Occurrence (배추좀나방의 계절적 발생과 월동집단의 유전적 분화 감소)

  • Kim, Eunseong;Park, Areum;Park, Youngjin;Kim, Jooil;Kim, Yonggyun
    • Korean journal of applied entomology
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    • v.54 no.4
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    • pp.303-310
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    • 2015
  • The diamondback moth, Plutella xylostella, overwinters in some protected areas in Korea. Using a sex pheromone trap, the adults were monitored since the occurrence of the overwintering populations. In Andong, P. xylostella exhibited four adult peaks in a year. Biological characters, such as cold tolerance, insecticide susceptibility, and developmental rate, were analyzed and showed a significant variation among different local overwintering populations. Population genetic variation was assessed with molecular markers, in which the initial high genetic variation among the overwintering populations decreased with the progress of seasons. These results suggests that there may be a significant migration of P. xylostella to decrease the genetic variation among the different local populations that are different in biological characters.

Clinical and Pathological Characteristics of Frontotemporal Lobar Degeneration(FTLD) and Molecular Genetics of Tau Protein (Frontotemporal Lobar Degeneration(FTLD)의 임상적, 병리적 특징과 타우 단백질의 분자 유전학)

  • Woo, Sung-Il
    • Korean Journal of Biological Psychiatry
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    • v.10 no.2
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    • pp.97-106
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    • 2003
  • Criticisms about amyloid cascade hypothesis of Alzheimer's disease(AD) are based on the findings, first, that the degree of dementia does not correlate with the number of plaques, and second, that the neurofibrillary tangle formation seems to predate plaque formation. In addition, neurofibrillary tangle counts correlate well with the degree of cognitive impairment. These findings suggest the independent importance of tau abnormality in AD research which is involved in the neurofibrillary tangle formation. Recently, tau pathology without amyloid deposits and mutations in tau protein gene were reported to be the major pathogenic mechanism in Pick's disease, progressive supranuclear palsy, corticobasal degeneration and FTDP-17(frontotemporal dementia and parkinsonism linked with chromosome 17). These data suggest that understanding the causes and consequences of tau dysfunction might give new clinical and therapeutic solutions to many known tauopathies.

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Therapeutic Appilication of Molecular Genetics in Psychiatry (정신과에서 분자유전학의 치료적 적용)

  • Lee, Min Soo
    • Korean Journal of Biological Psychiatry
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    • v.5 no.1
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    • pp.17-33
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    • 1998
  • Advances in molecular biology contribute to the understanding genetic mechanism of psychiatric disorders. They have renewed hope for the discovery of disease relevant gene. However, the results somewhat confused. And we will wait for a long time for the application of gene therapy in schizophreniar. Fortunately we could classified the schizophrenia with genotypes of dopamine and serotonin receptors. It is expected that this genetic classification could provide key strategy for the therapeutic application in biological treatment for schizophrenia. The purpose of this article is to call attention of the institute participants to linkage, association, mRNA expression, genotypic classification and to the need for more systemic research. The author summarized the modified methods which were done in his laboratory in appendix.

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Taxonomic Study of the Genus Candida by Cellular Fatty Acid Analysis (세포내 지방산 조성 분석에 따른 Candida속의 분류)

  • 신기선;홍순덕;배경숙
    • Korean Journal of Microbiology
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    • v.33 no.2
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    • pp.81-85
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    • 1997
  • Candida속 효모 27종이 표준 균주를 대상으로 세포내 지방산 조성을 기체 크로마토그래피로 분석하여, 함유 지방산의 종류와 함량비를 근거로 27종이 균주를 3개의 분류군으로 나누었다. 이들 지방산 분석에 의한 분류군을 Candida속의 다른 분류학적 결과들인 coenzyme Q 분자종의 종류, DNA 염기함량 분석, ITS 부위의 제한효소 절단 다형질의 양상, 리보좀 소단위 RNA 유전자의 염기배열 보고와 비교 분석하였다. 그 결과 같은 분류군에 위치하는 균주들 사이의 연관 관계가 거의 일치하였다. 다라서 세포내 지방산 분석은 Candida속 규준들을 유전적 동질성을 갖는 균주들로 분류하는 수단으로 사용될 수 있으며, 유전적으로 매우 다양한 균주들의 집단으로 알려진 Candida속에 관한 분류적 고찰에 일차적으로 사용될 수 있는 유용한 수단임을 알 수 있었다.

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