• The Korean Journal of Mycology
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• v.45 no.4
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• pp.355-361
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• 2017

In this study, endophytic fungi were isolated from the roots of three native orchid species from Korea: Bletilla striata, Oreorchis patens, and Cephalanthera longibracteata. The isolated fungal endophytes were identified based on the morphological and molecular characteristics including sequence analysis of internal transcribed spacer (ITS) and 28S ribosomal DNA regions. As a result, we discovered 5 species of fungal endophytes that have not been previously reported in Korea: Phialocephala bamuru, Coniochaeta mutabilis, Phialophora foetens, Calonectria canadensis, and Neonectria ramulariae.

• Journal of Wetlands Research
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• v.14 no.3
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• pp.429-438
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• 2012

Han River is very important as a source of drinking water in metropolitan area. This study was conducted to figure out diversity and dynamics of plankton community in the seven lakes (lakes Paro, Chunchon, Soyang, Uiam, Chungpyung, Paldang, Chungju) of Han Riversystem. A total of 76 genera and 121 species were investigated by taxonomic identification in November 2008. Cyclotella sp. and Microcystis sp. was a dominant species in lake Paro and Chungju respectively. Aulacoseira granulata was dominant in lakes Chunchon, Paldang and Chungpyung. And Fragilalia crotonesis was a dominant species in lake Soyang and Uiam. Our results can be useful when compared to the results using molecular biological method to supplement taxonomic identification.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.8
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• pp.3517-3522
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• 2011

Contamination of public bath water by waterborne pathogens can cause disease outbreaks and contribute to background rates of disease. The aim of this study is to determine the prevalence of waterborne pathogens in public baths. A total of 30 water samples were collected from 30 different public baths in seoul, Korea. Pathogens in water samples were concentrated by 0.45 ${\mu}m$ nitrocellulose membrane filter, analyzed by both cultivation and polymerase chain reaction-reverse blot hybridization (PCR-REBA) of partial 16S rRNA gene. Various microorganisms including Escherichia coli and Shigella spp. were identified by microbiological cultivation. E. coli, Shigella spp., Salmonella spp., Pseudomonas spp. and Mycobacterium spp. were identified by PCR-REBA. Our results suggest that appropriate hygiene practice and continuous monitoring is needed for reducing health risk associated with public bath houses.

• Proceedings of the Korean Aquaculture Society Conference
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• 2006.05a
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• pp.518-519
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• 2006

• Korean Journal of Environmental Biology
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• v.17 no.3
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• pp.321-330
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• 1999

Nematodes were isolated using silkwom trap through the investigation of 100 soil samples from various biotopes in Korea. The 30 nematode strains from soil and dead insects by the pathogenicity aganinst silkworms (Bombyx mori mori) and insect pests of Calliphora vomitoria, Pseufazetia separata, Palomena angulosa, and Melolontha incana. Mortailty of the silkworm larvae and pupae were as high as 100% by nematode infection, those of insect of pests were varied from 20 to 100%. The 30 strains of entemopathogenic nematodes were classified into five groups of Rhabditidae, Diplogatroidae, Heterorhabitidae, Steinernematidae, and Tylenchida by morphological criteria. The genetic relationships among the 30 nematode strains were analyzed by various RAPD bands with twenty primers. The 30 nematode strains were classified into six major subgroups on the basis of the genetic similarity coefficient of 0.853. The grouping by RAPD was agree with those of morphological taxa in discrimination of the higher group, however, was not completely agree in the subgroup. The family Steinernematidae belong to Rhabditida was clarified as closer to the Tylenchida, rather than the other Rhabditida of Heterorhabitidae, Rhabditidae, and Diplogatroidae in genetic distance valule. From the result of the morphological classification and RAPD of the genomic DNA showed that genetic relationship analysis furnish infurmation on phylogenetic classification and relationships of entomopathogenic nematodes. The application of genetic similarity will overcome the limitation of taxonomy and classification of morphologically simple nematode. Several primers were confirmed those utility of identification for individual nematode strains, the methods of molecular genetics secured the simplicity, rapidity and accuracy on the selection of entomopathogenic nematodes.

• Proceedings of the Korean Society of Soil and Groundwater Environment Conference
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• 2006.04a
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• pp.29-32
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• 2006

화학적 산화처리와 bioremedation 기법을 개별적 또는 복합적으로 동시에 적용함으로써 한 개별 기법의 단점을 보완하고 현장적용성을 증대시킬 수 있는 통합기법을 개발하고자 하였다. 펜톤유사 반응을 통해 고농도의 유류를 산화분해 시킨 후 미생물 처리를 통해 잔류 유류 오염물질을 제거하고자 하였다. 유류 오염토양의 화학 생물학적 통합처리 공정의 현장 적용성 및 토양 미생물에 미치는 영향을 검증하기 위해 처리과정 전 후의 미생물 군집구조를 분석하였다. 또한 토양 내 유류 분해균을 분리하기 위해 탄소원으로 경유와 벙커C를 이용하여 농화배양을 수행하였다. 경유 분해균 10여종, 벙커 C 분해균 6종을 분리하여 분해능 및 동정을 시도하였다. 또한 유류 분해미생물의 consortia를 분자생물학적 기법으로 분석을 시도하였다.

• Korean Journal of Clinical Laboratory Science
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• v.52 no.3
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• pp.229-236
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• 2020

Sixty-five molds isolated from clinical specimens were included in this study. All the isolates were molds that could be identified morphologically, strains that are difficult to identify because of morphological similarities, and strains that require species-level identification. PCR and direct sequencing were performed to target the internal transcribed spacer (ITS) region, the D1/D2 region, and the β-tubulin gene. Comparative sequence analysis using the GenBank database was performed using the basic local alignment search tool (BLAST) algorithm. The fungi identified morphologically to the genus level were 67%. Sequencing analysis was performed on 62 genera and species level of the 65 strains. Discrepancies were 14 (21.5%) of the 65 strains between the results of phenotypic and molecular identification. B. dermatitidis, T. marneffei, and G. argillacea were identified for the first time in Korea using the DNA sequencing method. Morphological identification is a very useful method in terms of the reporting time and costs in cases of frequently isolated and rapid growth, such as Aspergillus. When molecular methods are employed, the cost and clinical significance should be considered. On the other hand, the molecular identification of molds can provide fast and accurate results.

• Korean Journal of Ichthyology
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• v.31 no.4
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• pp.249-254
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• 2019

One specimen of Albula argentea (296.3 mm in standard length), belonging to the family Albulidae, was collected in a set net in the coastal waters of Jejudo Island, Korea. As the adult A. argentea has not been caught from Korean waters, we described its morphological characters based on the collected specimen and compared them with those of the previous reports on A. argentea and A. koreaus. As a result, the present specimen was characterized by having 68~73 pored lateral line scales (vs. 77~78 for A. koreana), 72~73 vertebrae pored lateral line scales (vs. 77~80) and none streak on the check (vs. yellowish streak). Molecular analysis using the mitochondrial cytochrome b DNA sequences was also conducted to confirm the correctness of species identification and taxonomic status of the sample.

• The Korean Journal of Mycology
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• v.43 no.1
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• pp.53-57
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• 2015

Ulleungdo is a volcanic island inhabited by a variety of animals and plants in an oceanic climate in the East Sea. The presence of filamentous fungi has been rarely reported in Ulleungdo. Thus, in this study some plants in the Nari basin and soils in the Dodong region were sampled. Fungi were isolated from the soil and identified by microscopical and molecular biological methods. Coniothyrium aleuritis, Hypocrea valdunensis, Pestalotiopsis uvicola and P. caudate were identified and found to be undescribed species in Korea.

• Journal of the Korea Organic Resources Recycling Association
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• v.29 no.3
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• pp.27-33
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• 2021

CO I gene sequence analysis was applied to earthworms that had been used as test animals in toxicity test in Institute of Kyeongbook Agrochemicals and earthworms used as vermicomposting agents in the farm of Youngdong province to identify their species names. In terms of molecular species, the former was identified as Eisenia fetida and the latter was Eisenia andrei. Cocoons produced from Eisenia fetida was more than those from Eisenia andrei. And No. of adults developed from eggs of Eisenia fetida was more or less higher than those developed from eggs of Eisenia andrei. These results were contradictory to previous reports on two Eisenia spp.. When Eisenia fetida was crossed with Eisenia andrei, hybridized eggs were produced and adults were developed from those eggs, but cocoons and adults were much less than those from non-crossed Eisenia fetida or Eisenia andrei. This indicated that two Eisenia spp. were not distinctly different biological species because there was no complete 'reproductive isolation' between Eisenia fetida and Eisenia andrei. However, this also meant that Eisenia fetida and Eisenia andrei had already been on the tract of speciation.