• The Transactions of The Korean Institute of Electrical Engineers
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• v.58 no.1
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• pp.147-154
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• 2009

This paper presents a grating~integrated SPR (Surface Plasmon Resonance) sensor chip for simple and inexpensive biomolecule detection. The grating-integrated SPR sensor chip has two sensing channels having a nano grating for SPR coupling. An external mirror is used for multi channel SPR sensing. The present sensor chip replaces bulky and expensive optical components, such as fiber-optic switches or special shaped prisms, resulting in a simple and inexpensive wavelength modulated multi-channel SPR sensing system. We fabricate a SPR sensor chip integrated with 835 nm-pitch gratings by a micromolding technique to reduce the fabrication cost. In the experimental characterization, the refractive index sensitivity of each sensing channel is measured as $321.8{\pm}8.1nm$/RI and $514.3{\pm}8.lnm$/RI, respectively. 0.5uM of the target biomolecule (streptavidin) was detected by a $1.13{\pm}0.16nm$ shift of the SPR dip in the 10%-biotinylated sample channel, while the SPR dip in the reference channel for environmental perturbation monitoring remained at the same position. From the experimental results, multi-channel biomolecule detection capability of the present grating-integrated SPR sensor chip has been verified. On the basis of the preliminary experiments, we successfully measured the binding reaction rate for the $2\;nM{\sim}200\;nM$ monoclonal-antibiotin, thus verifying biomolecule concentration detectability of the present SPR sensor chip. The binding reaction rates measured from the present SPR sensor chip agredd well with those from a commercialized SPR sensor.

• Journal of the Korean Applied Science and Technology
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• v.29 no.1
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• pp.122-128
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• 2012

The influences of fluorescence, scattering, and flocculation in turbid material by light scattering of L-aspartic acid, preservative and emulsifier were interpreted for the scattered fluorescence intensity and wavelength. They have been studied the molecular properties by the spectroscopy of laser induced fluorescence (LIF) and flocculation. The effects of optical properties in scattering media have been found by the optical parameters(${\mu}_s$, ${\mu}_a$, ${\mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used. When two particles approach each other, interactions of several colloid particles can come into play which may have major effect on the flocculation and LIF process. The values of scattering coefficient ${\mu}_s$ are large by means of the increasing scattering of scatterer, The values have been found that the slope decays exponentially as a function of concentration from laser source to detector by our experimental result. It may also aid in designing the best model for oil chemistry, bio-pharmaceutical products, laser medicine and application of medical engineering on LIF and coagulation in particle transport mode.

• Journal of the Korean Applied Science and Technology
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• v.31 no.2
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• pp.167-175
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• 2014

The influences of fluorescence, scattering, and flocculation in turbid material were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by the spectroscopy of laser induced fluorescence(LIF). The effects of optical properties in scattering media have been found by the optical parameters(${\mu}_s$, ${\mu}_a$, ${\mu}_t$). Flocculation is an important step in many solid-liquid separation processes and is widely used in Photodynamic therapy. The interactions of several colloid particles can come into play which have major effect on the flocculation and LIF process. We measured scattering and fluorescence spectra of the sample for in vitro as function of concentration from lase source to detector. The value of scattering coefficient ${\mu}_s$ is large by means of the increasing particles of scatterer. Therefore, Phorphyrin A is larger than Phorphyrin C in scattering intensity ${\mu}_s$, but Phorphyrin A is smaller than Phorphyrin C in penetration depth ${\delta}$.

• Journal of the Korean Applied Science and Technology
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• v.30 no.2
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• pp.251-257
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• 2013

The influences of Methotrexate as fluorophor, scatterer, absorber in turbid material by light scattering were interpreted for the scattered fluorescence intensity and wavelength, it has been studied the molecular properties by laser induced fluorescence spectroscopy. It has been found that the effects of optical properties in scattering media by the optical parameters((${\mu}_s$, ${\mu}_a$, ${\mu}_t$). The value of scattering coefficient ${\mu}_s$ is large by means of the increasing particles of L-${\alpha}$-Phosphatidylcholine, it has been found that the slope decays exponentially as a function of depth from laser source to detector. It may also aid in designing the best model for oil chemistry, laser medicine and application of medical engineering.

• Analytical Science and Technology
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• v.8 no.2
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• pp.139-159
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• 1995

Positive ion mass spectra of some corticosteroids were obtained by using liquid chromatography-mass spectrometry(LC-MS). The base peak of each compound showed the protonated molecular ion [$MH^+$], ammonium adduct ion [${MNH_4}^+$] or [$MH^+-60$] ion according to its chemical structure and other characteristic mass ions were [$MH^+-18$], [${MNH_4}^+-18$] and so on. Several rat urinary metabolites of methylprednisolone acetate after the oral administration were detected by the thermospray LC-MS. The identified major metabolites were 20-hydroxymethylprednisolone(20-HMP), methylprednisolone(MP) and methylprednisone(11-KMP), which were supposed to be formed by deacetylation at the position of C-21, reduction at C-20, oxidation at C-11, or due to the bond cleavage between C-17 and C-20.

• Analytical Science and Technology
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• v.23 no.5
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• pp.446-456
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• 2010

In this study, the performance characteristics of DNPH sampling were investigated in the collection and analysis of 5 carbonyl compounds (CC) in air using the cartridge products produced by three different makers. For these experiments, gaseous standards of 5 CCs were prepared to cover 9 concentration levels for each compound (33~2600 nmol). Some cartridge products exhibited relatively high blank values of acetaldehyde (AA) and propionaldehyde (PA). The recovery rates of all three cartridges showed moderate reduction as the molecular weight of CC increased. In addition, when the recovery rate was compared by percent error (%), the most stable patterns were achieved in the intermediate concentration range of 263~1312 nmol (in case of AA). The overall results of our study suggest that the optimal range of recovery for a given concentration range should be considered to obtain the most reliable data for the DNPH cartridge method.

• Journal of Mushroom
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• v.17 no.4
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• pp.185-190
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• 2019

Pleurotus ostreatus No.42, known as the ligninolytic basidiomycetes, showed production of MnP and Lac, but did not show any LiP acitivity in static culture, grown in GPYW liquid medium. Maximum production of MnP (80U/flask) was observed on day 11 of culturing in this medium. Chromatographic purification of MnP included the use of Sepharose CL-6B and Mono-Q. The major MnP isozyme purified by column chromatography was observed to be a 36.4 KDa (single band on SDS PAGE). The 19-amino acid sequence from the N-terminal was determined by protein sequencing to be ATCADGRTTANAACCVLFP. The N-terminal sequence of the major MnP isozyme of P. ostreatus No.42 was found to be the same as a previously reported sequence of an MnP3 isozyme from this fungus.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.211-212
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• 2011

플라즈마를 제어하기 위해서는 플라즈마의 온도, 밀도, 에너지 분포등과 같은 플라즈마의 특성을 정확히 측정할 수 있어야한다. 핵융합발전에서는 플라즈마를 발생하기 위하여 플라즈마의 온도, 밀도 등 각종 변수들을 시공간적으로 계측, 분석할 수 있는 진달설비를 사용하고 있으며, 정확한 플라즈마 제어와 측정을 위한 새로운 진단기술을 개발하고 있다. 그리고 중요한 변수중에 하나인 플라즈마 이온온도를 측정하기 위해 중성입자 검출법이 잘 알려져 있다. 이 실험은 수소 중성입자가 토카막 내부의 플라즈마 이온과 충돌하면서 생성된 고속 중성입자의 에너지를 분석하는 실험이다. 본 연구의 실험방법은 수소 중성입자를 이온빔 장치에서 이온화 시킨 후 자체 제작한 가속기를 통하여 가속시켜 에너지 특성을 분석을 하는 것이다. 본 연구의 실험장치로 에너지 교정용 100 keV 이온빔 소스를 제작 하였고 이온빔 장치 내부에 수소기체를 주입하고 기체방전을 일으켜 플라즈마를 발생시켰다. 이온빔 외부에는 팬을 설치하고 전도성이 강한 물 대신 전도성이 약한 오일을 사용하여 냉각 하였다. 이온빔 장치와 결합될 이온 가속장치는 지름 300 mm, 두께 2 mm의 원형 구리판을 여러층으로 쌓아 전극으로 제작하였고 전극과 전극 사이에서 코로나 방전과 스파크를 방지하기 위해 전극 둘레에 코로나링을 설치 하였다. 또한 전극 사이마다 1G${\Omega}$의 저항을 설치한 후 고전압을 생성하여 이온 가속 효율을 증대시켰다. 진공시스템으로는 Alcatel사의 CFF100 터보분자 펌프와 우성진공사의 MVP24 진공로타리펌프를 결합하여 사용하였으며, 진공도측정은 Alcatel사의 ACS1000 장치를 사용하였다. 고진공후 고속 중성입자의 이온화와 에너지 측정을 위한 전하교환기를 설치하였다. 전하교환기로는 진공시스템을 별도로 설치하고 비용이 비교적 많이 드는 기체형 전하교환기 대신 소형화가 가능하고 유지보수가 좋은 고체형 전하교환기 제작하여 실험 하였다. 전하교환기에서 이온화된 고속 중성입자가 전기장이나 자장에 영향을 받았을때 에너지분포를 디텍터를 통해 측정하였다. 즉, 이온화된 중성입자의 에너지가 실리콘 다이오드를 통해 전압 펄스 신호로 변환되고 이차 증폭기를 통해 전압 펄스 신호들이 증폭한다. 에너지 측정을 위한 디텍터는 소형화가 가능하고 비용이 비교적 적게 드는 실리콘 다이오드를 설치하였다. 본 연구결과 중성입자 에너지 분석 장치가 실제 핵융합 장치의 플라즈마 이온온도와 특성 측정에 적용할 수 있으며, 앞으로 개발될 여러 형태의 응용 플라즈마 발생장치의 플라즈마 진단에 이용될 것으로 기대한다.

• The Korean Journal of Food And Nutrition
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• v.14 no.1
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• pp.20-27
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• 2001

The consumption of radish ( Rhaphanus sativus L.) sprout, which is Cruciferae family, is increasing because of its pungent flavor and taste. Its volatile components were analyzed by SDE (simultaneous steam distillation & extraction) method and P&T(purge & cryogenic trapping) method. As a solvent, diethyl ether and diethyl ether : pentane mixture(2:1, v/v) were used in SDE method, and diethyl ether in P&T method. Analyzing by GC and GC-MS, the major component was sulfur compounds (19 species, peak area 76.6%) with diethyl ether, sulfur compounds(15. 44.0%) and hydrocarbons(23, 23.8%) with diethyl ether-pentane mixture in SDE method. Also, hydrocarbons(25, 84.1% ) was major component in P& T method. The major volatile component of fresh radish sprout were n-heptane, methyl pentane and that of boiled radish sprout were 4-methylthio-3-butenyl isothiocyanate, methyl mercaptane, 2,3-dimethyl disulfide. Low molecular volatile components were detected more by P& T method, but types and relative quantities of volatile components were measured less comparing to SDE method.

• Journal of the Korean Chemical Society
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• v.13 no.4
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• pp.355-364
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• 1969

Cross-hybridizations between DNA of two pseudomonads and a xanthomonad suggested that the three DNA types had a considerable section in common. The existence of this common part was proved by hybridization of preselected DNA, i.e. DNA resulting from a previous hybridization between any one set of two DNA types, with the third type. It was thus shown that about 50% of the DNA of the three organisms was similar. This common part was isolated in pure state and its % (G+C) was found to be indentical to the overall base composition of the native DNA. The evolutionary drift in % (G+C) could thus not be detected. The total molecular weight of the chromosornal DNA/bacterial nucleoid was determined to be 2.4 ${\times} 10^9$daltons. It can therefore be estimated that the common putida-fluorescenspelargonii DNA part consists of some 2,000 cistrons. P. putida and P. fluorescens share an additional 1,300 cistrons, and all xanthomonads share at least an additional 1,000 cistrons.