• Proceedings of the Korean Society of Fisheries Technology Conference
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• 2001.05a
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• pp.140-141
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• 2001

젓갈의 제조 공정 중 미생물의 작용은 주로 숙성과정과 완제품의 보관과정에 일어나는 것으로 알려져 있다. 우선 숙성과정은 원료와 함께 부착되어온 미생물이 일차적으로 식염농도에 따라 선택을 받아 적응해 가면서 2차적인 대사산물 생성 및 적자 생존에 의하여 우점종을 형성하여 발효에 관여하게 되며, 젓갈은 그 특성상 멸균과정을 거칠 수 없어 숙성에 관여했던 미생물들이 완제품의 보관 중에도 계속 남아있게 되므로 저장성에 있어 문제가 되고 있다. (중략)

• Journal of Korean Society of Environmental Engineers
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• v.31 no.10
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• pp.855-864
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• 2009

The effect of toxicant on the inhibition of nitrification was investigated, using concentrated nitrifying bacteria of both attached and suspended growth. This nitrifying organism was originally obtained from the activated sludge of sewage treatment plant and cultivated for more than three months. The object of this experiment is to determine the effect of the specific surface area and the growth condition of nitrifying bacteria on toxicity of heavy metal. The results of this study were as follows. The specific surface area of both attached and suspended growth of nitrifying organism was proven to be a major factors in determining the inhibition of nitrification of heavy metal such as $Cu^{++}$ion. When the condition of attachment and detachment was compared in an experiment using attached growth nitrifier, the effect on toxicant was 1.12 times less in attached condition than in detached condition for Nitrosomonas, and 1.09 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.46 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.35 times less for Nitrobacter. Also, similar results were obtained in a set of experiments, without adding nitrite to the substrate. In an experiment that compared attached condition using attached growth nitrifier with detached condition using attached growth nitrifier, the effect on toxicant was 1.83 times less in attached condition than in detached one for Nitrosomonas, and 1.78 times less for Nitrobacter. In case of suspended growth nitrifier, the effect on toxicant was 1.27 times less in non-ground condition than in ground condition for Nitrosomonas, and 1.32 times less for Nitrobacter.

• Korean Journal of Microbiology
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• v.41 no.1
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• pp.47-52
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• 2005

This study was carried out to identify bacterial strains adhered to human scalp hair and to investigate the antibiotic susceptibility of them. A total of 39 isolates were obtained from patients in intensive care units and healthy persons. The most common species isolated was Staphylococcus epidermidis (19 isolates), followed by S. aureus (14 isolates), S. waneri (5 isolates), and S. pasteuri (1 isolate). The susceptibility of isolates to amikacin, ampicillin, bacitracin, carbenicillin, cefazolin, cefoperazone, chloramphenicol, erythromycin, gentamicin, methicillin, nalidixic acid, neomycin, oxacillin, penicillin, streptomycin, tetracycline and vancomycin was determined by the disk diffusion method. All of the antibiotic resistant isolates were obtained from patient scalp hair. To examine the effect of conventional shampoo and detergent SDS on removing of bacteria from hair, we treated hair with culture solution of S. aureus. The bacteria attached to hair were not removed even by repeated washing with detergents. These results suggested that hair could be a source of bacterial contamination in hospital.

• Journal of Korean Society of Environmental Engineers
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• v.29 no.4
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• pp.452-459
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• 2007

Nutrient removal hybrid process to use suspended and attached growth microorganisms and apply the contact and stabilization process was process obtaining good results to HRT within 6 hours to dominate nitrifier and to promote separation and growth of autotrophs and heterotrophs to pack with EPS(Expanded Poly-Styrene) media in nitrification reactor. An average effluent quality of this process was below 5.2 mg/L, 7.3 mg/L, 4.9 mg/L as $BOD_5,\;COD_{Mn}$, SS concentration and 6.8 mg/L, 0.6 mg/L as T-N, T-P concentration. Also, An average removal efficiency of this process was 94.6%, 79.8%, 94.9% as $BOD_5,\;COD_{Mn}$, SS and 70.8%, 76.9% as T-N, T-P. 16S-rRNA analysis result of microorganisms attached to EPS media was researched Nitrosomonas and Nitrosococcus blown to ammonia-oxidizing bacteria cluster to include Gallionella and these microorganisms were researched to involve about 6% of biofilm attached EPS media. Consequently, this process was treated below 10 mg/L and 1.0mg/L as T-N, T-P concentration at short hydraulic retention time(about 6 hr) to dominate nitrifier.

• Journal of Korean Society of Water and Wastewater
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• v.20 no.3
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• pp.461-467
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• 2006

The seasonal change in attached algae and microbial community structure at sedimentation basin of water treatment plant was investigated by using quinone profiles and denaturing gel gradient electrophoresis (DGGE). The photosynthetic bacteria and algae contains PQ-9 and VK-1 as major quinone are major component of the total quinone fraction in attached algae and microorganisms on sedimentation basin trough. The microorganisms containing menaquinones appear to be sensitivity to the change in temperature than those containing ubiquinones. The plot of the mole fraction of dominant quinone species ($f_d$) to the DQ values showed higher sensitivity to the seasonal change in the microbial community structure. The results indicated that quinone and DGGE are useful tool for the evaluation of the changes in the microbial community structure.

• KSBB Journal
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• v.16 no.3
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• pp.225-232
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• 2001

The hydrophobicity of the microbial cell surface is responsible for the various interactions between microorganisms and different surface, and results in the flocculation of microbial cells, their adhesion to liquid or solid materials, and the floatation of microorganisms at the air-water interface. Accordingly, cell surface hydrophobicity is important not only in medicine but in other areas of biotechnology. This article reviews the role of cell surface hydrophobicity and its applications.

• Korean Journal of Microbiology
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• v.38 no.3
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• pp.192-197
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• 2002

Fluorescent in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes was used to compare the community structures of free-living and aggregated bacteria at thawing period in Lake Baikal. Targeted groups were Eubacteria, $\alpha$-, $\beta$-, $\gamma$- proteobacteria groups, Cytophaga-Flavobacterium group and Planctomycetales. Total bacterial numbers of free-living bacteria were ranged from $0.2{\times}10^6\cells{\cdot}ml^-1$ to $3.2{\times}10^6\cells{\cdot}ml^-1$, which were decreasing with depth, while the aggregated bacterial numbers were dramatically increasing from $0.4{\times}10^4 to 3.3{\times}10^4 \cells{\cdot}ml^-1$ with depth. The ratios of EUB probe binding cells to DAPI counts were ranged from 52.3 to 74.1% in free-living bacteria, and from 39.6 to 66.7% in the aggregated bacteria, respectively. Community structures of the aggregated bacteria were very different from each free-living bacteria at every depth. At 25 m depth, where the chlorophyll a concentration was highest, both structures were quite different from those of surface layers, rendering the fact that the community structures might be affected by phytoplankton. The vertical profile of community structure of aggregated bacteria is particular. The proportion of $\beta$-proteobacteria group was increasing with depth and it was 51.8% at 100 m, but the dominant group was $\gamma$-pro-teobacteria group at 250 m. Taken together, the biodiversity and succession of aggregated bacteria are quite different from free-living bacteria.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2006.11a
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• pp.357-361
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• 2006

• KSBB Journal
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• v.18 no.3
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• pp.243-247
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• 2003

The microbial immobilization media with curdlan and activated carbon which has great immobilization capacity has been developed. Characteristics of porosity and mechanical strength of this support media are dependent on manufacturing method. The support media showed the best cell immobilization performance when the ratio of curdlan and activated carbon was 30 g/L to 6 g/L in this study. The immobilization of iron-oxidizing bacteria on the supporting particles was photographed with a scanning electron microscope. Since cell concentration on the surface of supporting particle increased with the reaction time, the iron oxidation rate also increased.

• Korean Journal of Microbiology
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• v.40 no.2
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• pp.115-120
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• 2004

A Gram (-), rod-shaped bacterium in size of 1.3∼$1.8{\times}0.35{\mu}m$ inhibiting the growth of cyanobacterium (Ana-baena cylindrica) was isolated and designated NG-2 in this manuscript. This isolate showed positive reactions for catalase and oxidase, and optimal growth conditions of 35∼TEX>$40<^{\circ}C$ and pH 9.0. In a mixed-culture of A. cylindrica and the isolate, each microorganism grew inverse-proportionally, and the cyanobacterial vegetative cells almost completely disappeared within 24 hours. NG-2 lysed A. cylindrica only under light, which means that lytic activity of NG-2 was dependent on the photosynthetic activity of host. When observed under phase contrast microscope, the isolate lysed vegetative cells of A. cylindrica in scattered state in a liquid medium, whereas het-erocysts have not been lysed. When cyanobacterial cell walls have been lysed partly, NG-2 attatched around A. cylindrica filament and formed colony, then encouraged complete lysis of cyanobacterial cells. The isolate showed similar lytic activity in natural water as in an artificial medium. And lytic activity of NG-2 was enhanced when attached on expandable polystyrene bead.